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For research use only and NOT intended for in vitro diagnostics.
For the rapid purification of genomic DNA from various bacterial species found in milk
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Milk Bacterial DNA Isolation Kit
This kit provides a rapid spin column method for the isolation and purification of genomic DNA from both Gram-negative and Gram-positive bacteria in milk samples.  It can also be used to process challenging milk samples such as Mastitic milk for example. The kit is highly sensitive and can isolate DNA from a cell density of as little as 10 bacteria contained in 1 mL of milk.  Genomic DNA is isolated in 45 minutes, free of inhibitors and ready for any number of downstream applications including PCR, qPCR and Southern Blot analysis, sequencing and more.​
Kit Specifications
|
|
Maximum Milk Input
|
1 mL
|
Minimum Detection Limit
|
10 bacteria in 1 mL of milk
|
Bacteria Species Processed
|
Gram positive and Gram negative
|
Time to Complete 10 Purifications |
1 hour
|
Â
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The Lysozyme should be stored at -20°C upon arrival, and the Resuspension Solution A should be stored at -20°C after addition of the lysozyme. The lyophilized Proteinase K should be stored at -20°C upon arrival and after reconstitution. These reagents should remain stable for at least 1 year in their unopened containers.Â
Title | Bacterial ecology of artisanal Minas cheeses assessed by culture-dependent and -independent methods |
Journal | Food Microbiology. 2017 |
Authors | Luana Martins Perin, Maria Luisa Savo Sardaro, LuÃs Augusto Nero, Erasmo Neviani, Monica Gatti |
Title | Microbiota network and mathematic microbe mutualism in colostrum and mature milk collected in two different geographic areas: Italy versus Burundi |
Journal | ISME Journal. 2016 |
Authors | Drago L, Toscano M, De Grandi R, Grossi E, Padovani EM, Peroni DG |
Title | Treating Generational Stress: Effect of Paternal Stress on Development of Memory and Extinction in Offspring Is Reversed by Probiotic Treatment |
Journal | Psychological Science. 2016 |
Authors | Callaghan BL, Cowan CSM, Richardson R |
Title | Molecular diagnosis of bovine tuberculosis in bovine and human samples: implications for zoonosis |
Journal | Future Microbiology. 2015 |
Authors | Md Masudur Rahman, Monira Noor, Kazi Mehetazul Islam, Md Bashir Uddin, Ferdaus Mohd Altaf Hossain, Mohammad Ali Zinnah, Mohammad Al Mamun, Mohammad Rafiqul Islam, Seong Kug Eo & Hossam M Ashour |
Title | Visualizing the Indefinable: Three-Dimensional Complexity of ‘Infectious Diseases’ |
Journal | PLOS ONE. 2015 |
Authors | Gabriel Leitner, Shlomo E. Blum, Ariel L. Rivas |
Title | Analysis of raw goat milk microbiota: Impact of stage of lactation and lysozyme on microbial diversity |
Journal | Food Microbiology. 2014 |
Authors | Elizabeth A. McInnis, Karen M. Kalanetra, David A. Mills, Elizabeth A. Maga |
Title | Molecular analysis of spoilage-related bacteria in pasteurized milk during refrigeration by PCR and denaturing gradient gel electrophoresis |
Journal | Journal of Food Protection. 2009 |
Authors | He H, Dong J, Lee CN, Li Y |
Title | Repeat-based subtyping and grouping of Staphylococcus aureus |
Journal | Diagnostic Microbiology and Infectious Disease. 2009 |
Authors | Said KB, Ramotar K, Zhu G, Zhao X. |
Want a discount?
Create your account to receive 15% off on your first purchase!
Milk Bacterial DNA Isolation Kit
This kit provides a rapid spin column method for the isolation and purification of genomic DNA from both Gram-negative and Gram-positive bacteria in milk samples.  It can also be used to process challenging milk samples such as Mastitic milk for example. The kit is highly sensitive and can isolate DNA from a cell density of as little as 10 bacteria contained in 1 mL of milk.  Genomic DNA is isolated in 45 minutes, free of inhibitors and ready for any number of downstream applications including PCR, qPCR and Southern Blot analysis, sequencing and more.​
Kit Specifications
|
|
Maximum Milk Input
|
1 mL
|
Minimum Detection Limit
|
10 bacteria in 1 mL of milk
|
Bacteria Species Processed
|
Gram positive and Gram negative
|
Time to Complete 10 Purifications |
1 hour
|
Â
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The Lysozyme should be stored at -20°C upon arrival, and the Resuspension Solution A should be stored at -20°C after addition of the lysozyme. The lyophilized Proteinase K should be stored at -20°C upon arrival and after reconstitution. These reagents should remain stable for at least 1 year in their unopened containers.Â
Title | Bacterial ecology of artisanal Minas cheeses assessed by culture-dependent and -independent methods |
Journal | Food Microbiology. 2017 |
Authors | Luana Martins Perin, Maria Luisa Savo Sardaro, LuÃs Augusto Nero, Erasmo Neviani, Monica Gatti |
Title | Microbiota network and mathematic microbe mutualism in colostrum and mature milk collected in two different geographic areas: Italy versus Burundi |
Journal | ISME Journal. 2016 |
Authors | Drago L, Toscano M, De Grandi R, Grossi E, Padovani EM, Peroni DG |
Title | Treating Generational Stress: Effect of Paternal Stress on Development of Memory and Extinction in Offspring Is Reversed by Probiotic Treatment |
Journal | Psychological Science. 2016 |
Authors | Callaghan BL, Cowan CSM, Richardson R |
Title | Molecular diagnosis of bovine tuberculosis in bovine and human samples: implications for zoonosis |
Journal | Future Microbiology. 2015 |
Authors | Md Masudur Rahman, Monira Noor, Kazi Mehetazul Islam, Md Bashir Uddin, Ferdaus Mohd Altaf Hossain, Mohammad Ali Zinnah, Mohammad Al Mamun, Mohammad Rafiqul Islam, Seong Kug Eo & Hossam M Ashour |
Title | Visualizing the Indefinable: Three-Dimensional Complexity of ‘Infectious Diseases’ |
Journal | PLOS ONE. 2015 |
Authors | Gabriel Leitner, Shlomo E. Blum, Ariel L. Rivas |
Title | Analysis of raw goat milk microbiota: Impact of stage of lactation and lysozyme on microbial diversity |
Journal | Food Microbiology. 2014 |
Authors | Elizabeth A. McInnis, Karen M. Kalanetra, David A. Mills, Elizabeth A. Maga |
Title | Molecular analysis of spoilage-related bacteria in pasteurized milk during refrigeration by PCR and denaturing gradient gel electrophoresis |
Journal | Journal of Food Protection. 2009 |
Authors | He H, Dong J, Lee CN, Li Y |
Title | Repeat-based subtyping and grouping of Staphylococcus aureus |
Journal | Diagnostic Microbiology and Infectious Disease. 2009 |
Authors | Said KB, Ramotar K, Zhu G, Zhao X. |