Milk Bacterial DNA Isolation Kit

Cat. 21550
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Milk Bacterial DNA Isolation Kit

Cat. 21550

For the rapid purification of genomic DNA from various bacterial species found in milk

  • Genomic DNA can be isolated from as few as 10 bacterial cells in 1 mL of milk
  • Isolate genomic DNA from both Gram-negative and Gram-positive bacteria in milk
  • Can process challenging samples such as mastitic milk
  • Inhibitor-free DNA is ready for PCR, qPCR, Southern Blot, sequencing & more
  • Fast and efficient spin-column format

For research use only and NOT intended for in vitro diagnostics.

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Milk Bacterial DNA Isolation Kit
(Cat. 21550)
50 preps

Kit Size

  • Genomic DNA can be isolated from as few as 10 bacterial cells in 1 mL of milk
  • Isolate genomic DNA from both Gram-negative and Gram-positive bacteria in milk
  • Can process challenging samples such as mastitic milk
  • Inhibitor-free DNA is ready for PCR, qPCR, Southern Blot, sequencing & more
  • Fast and efficient spin-column format

Milk Bacterial DNA Isolation Kit

This kit provides a rapid spin column method for the isolation and purification of genomic DNA from both Gram-negative and Gram-positive bacteria in milk samples.  It can also be used to process challenging milk samples such as Mastitic milk for example.  The kit is highly sensitive and can isolate DNA from a cell density of as little as 10 bacteria contained in 1 mL of milk.  Genomic DNA is isolated in 45 minutes, free of inhibitors and ready for any number of downstream applications including PCR, qPCR and Southern Blot analysis, sequencing and more.

Kit Specifications

You have selected: Cat. 21550
Kit Specifications
Maximum Milk Input 1 mL
Time to Complete 10 Purifications 1 hour
DNA Yield* 500 ng to 8 μg
Bacteria Species Processed Gram positive and Gram negative
Minimum Detection Limit 10 bacteria in 1 mL of milk

*The range of the DNA yield will vary depending upon a number of factors including bacterial species and type of milk (fat content and %)

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The Lysozyme should be stored at -20°C upon arrival, and the Resuspension Solution A should be stored at -20°C after addition of the lysozyme. The lyophilized Proteinase K should be stored at -20°C upon arrival and after reconstitution. This kit is stable for 1 year from the date of shipment.

Supporting Data

Click for expanded view

Component Cat. 21550 (50 preps)
Resuspension Solution A 6 mL
Buffer SK 60 mL
Wash Solution A 18 mL
Elution Buffer B 15 mL
Proteinase K 12 mg
Lysozyme (powder) 120 mg
Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1

Title The microbial composition of kefir fermented from raw milk
Journal The microbial composition of kefir fermented from raw milk. 2022
Authors Luuk van Ooijen, Ton Baars and Remco Kort
Title Bacterial ecology of artisanal Minas cheeses assessed by culture-dependent and -independent methods
Journal Food Microbiology. 2017
Authors Luana Martins Perin, Maria Luisa Savo Sardaro, Luís Augusto Nero, Erasmo Neviani, Monica Gatti
Title Microbiota network and mathematic microbe mutualism in colostrum and mature milk collected in two different geographic areas: Italy versus Burundi
Journal ISME Journal. 2016
Authors Drago L, Toscano M, De Grandi R, Grossi E, Padovani EM, Peroni DG
Title Molecular diagnosis of bovine tuberculosis in bovine and human samples: implications for zoonosis
Journal Future Microbiology. 2015
Authors Md Masudur Rahman, Monira Noor, Kazi Mehetazul Islam, Md Bashir Uddin, Ferdaus Mohd Altaf Hossain, Mohammad Ali Zinnah, Mohammad Al Mamun, Mohammad Rafiqul Islam, Seong Kug Eo & Hossam M Ashour
Title Visualizing the Indefinable: Three-Dimensional Complexity of ‘Infectious Diseases’
Journal PLOS ONE. 2015
Authors Gabriel Leitner, Shlomo E. Blum, Ariel L. Rivas
Title Analysis of raw goat milk microbiota: Impact of stage of lactation and lysozyme on microbial diversity
Journal Food Microbiology. 2014
Authors Elizabeth A. McInnis, Karen M. Kalanetra, David A. Mills, Elizabeth A. Maga
Title Repeat-based subtyping and grouping of Staphylococcus aureus
Journal Diagnostic Microbiology and Infectious Disease. 2009
Authors Said KB, Ramotar K, Zhu G, Zhao X.

Milk Bacterial DNA Isolation Kit

This kit provides a rapid spin column method for the isolation and purification of genomic DNA from both Gram-negative and Gram-positive bacteria in milk samples.  It can also be used to process challenging milk samples such as Mastitic milk for example.  The kit is highly sensitive and can isolate DNA from a cell density of as little as 10 bacteria contained in 1 mL of milk.  Genomic DNA is isolated in 45 minutes, free of inhibitors and ready for any number of downstream applications including PCR, qPCR and Southern Blot analysis, sequencing and more.

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 21550
Kit Specifications
Maximum Milk Input 1 mL
Time to Complete 10 Purifications 1 hour
DNA Yield* 500 ng to 8 μg
Bacteria Species Processed Gram positive and Gram negative
Minimum Detection Limit 10 bacteria in 1 mL of milk

*The range of the DNA yield will vary depending upon a number of factors including bacterial species and type of milk (fat content and %)

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The Lysozyme should be stored at -20°C upon arrival, and the Resuspension Solution A should be stored at -20°C after addition of the lysozyme. The lyophilized Proteinase K should be stored at -20°C upon arrival and after reconstitution. This kit is stable for 1 year from the date of shipment.

Component Cat. 21550 (50 preps)
Resuspension Solution A 6 mL
Buffer SK 60 mL
Wash Solution A 18 mL
Elution Buffer B 15 mL
Proteinase K 12 mg
Lysozyme (powder) 120 mg
Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1

Title The microbial composition of kefir fermented from raw milk
Journal The microbial composition of kefir fermented from raw milk. 2022
Authors Luuk van Ooijen, Ton Baars and Remco Kort
Title Bacterial ecology of artisanal Minas cheeses assessed by culture-dependent and -independent methods
Journal Food Microbiology. 2017
Authors Luana Martins Perin, Maria Luisa Savo Sardaro, Luís Augusto Nero, Erasmo Neviani, Monica Gatti
Title Microbiota network and mathematic microbe mutualism in colostrum and mature milk collected in two different geographic areas: Italy versus Burundi
Journal ISME Journal. 2016
Authors Drago L, Toscano M, De Grandi R, Grossi E, Padovani EM, Peroni DG
Title Molecular diagnosis of bovine tuberculosis in bovine and human samples: implications for zoonosis
Journal Future Microbiology. 2015
Authors Md Masudur Rahman, Monira Noor, Kazi Mehetazul Islam, Md Bashir Uddin, Ferdaus Mohd Altaf Hossain, Mohammad Ali Zinnah, Mohammad Al Mamun, Mohammad Rafiqul Islam, Seong Kug Eo & Hossam M Ashour
Title Visualizing the Indefinable: Three-Dimensional Complexity of ‘Infectious Diseases’
Journal PLOS ONE. 2015
Authors Gabriel Leitner, Shlomo E. Blum, Ariel L. Rivas
Title Analysis of raw goat milk microbiota: Impact of stage of lactation and lysozyme on microbial diversity
Journal Food Microbiology. 2014
Authors Elizabeth A. McInnis, Karen M. Kalanetra, David A. Mills, Elizabeth A. Maga
Title Repeat-based subtyping and grouping of Staphylococcus aureus
Journal Diagnostic Microbiology and Infectious Disease. 2009
Authors Said KB, Ramotar K, Zhu G, Zhao X.

 


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