Milk Bacterial DNA Isolation Kit (Cat. 21550)
Milk Bacterial DNA Isolation Kit
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Milk Bacterial DNA Isolation Kit
For the rapid purification of genomic DNA from various bacterial species found in milk
- Genomic DNA can be isolated from as few as 10 bacterial cells in 1 mL of milk
- Isolate genomic DNA from both Gram-negative and Gram-positive bacteria in milk
- Can process challenging samples such as mastitic milk
- Inhibitor-free DNA is ready for PCR, qPCR, Southern Blot, sequencing & more
- Fast and efficient spin-column format
For research use only and NOT intended for in vitro diagnostics.
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(Cat. 21550) 50 preps
Product Format Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.
Product Size
Kit Specifications
|
Kit Specifications
|
|
| Maximum Milk Input | 1 mL |
| Time to Complete 10 Purifications | 1 hour |
| DNA Yield* | 500 ng to 8 μg |
| Bacteria Species Processed | Gram positive and Gram negative |
| Minimum Detection Limit | 10 bacteria in 1 mL of milk |
*The range of the DNA yield will vary depending upon a number of factors including bacterial species and type of milk (fat content and %)
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The Lysozyme should be stored at -20°C upon arrival, and the Resuspension Solution A should be stored at -20°C after addition of the lysozyme. The lyophilized Proteinase K should be stored at -20°C upon arrival and after reconstitution. This kit is stable for 1 year from the date of shipment.
Supporting Data
| Title | The microbial composition of kefir fermented from raw milk |
| Journal | The microbial composition of kefir fermented from raw milk. 2022 |
| Authors | Luuk van Ooijen, Ton Baars and Remco Kort |
| Title | Bacterial ecology of artisanal Minas cheeses assessed by culture-dependent and -independent methods |
| Journal | Food Microbiology. 2017 |
| Authors | Luana Martins Perin, Maria Luisa Savo Sardaro, LuÃs Augusto Nero, Erasmo Neviani, Monica Gatti |
| Title | Microbiota network and mathematic microbe mutualism in colostrum and mature milk collected in two different geographic areas: Italy versus Burundi |
| Journal | ISME Journal. 2016 |
| Authors | Drago L, Toscano M, De Grandi R, Grossi E, Padovani EM, Peroni DG |
| Title | Treating Generational Stress: Effect of Paternal Stress on Development of Memory and Extinction in Offspring Is Reversed by Probiotic Treatment |
| Journal | Psychological Science. 2016 |
| Authors | Callaghan BL, Cowan CSM, Richardson R |
| Title | Molecular diagnosis of bovine tuberculosis in bovine and human samples: implications for zoonosis |
| Journal | Future Microbiology. 2015 |
| Authors | Md Masudur Rahman, Monira Noor, Kazi Mehetazul Islam, Md Bashir Uddin, Ferdaus Mohd Altaf Hossain, Mohammad Ali Zinnah, Mohammad Al Mamun, Mohammad Rafiqul Islam, Seong Kug Eo & Hossam M Ashour |
| Title | Visualizing the Indefinable: Three-Dimensional Complexity of ‘Infectious Diseases’ |
| Journal | PLOS ONE. 2015 |
| Authors | Gabriel Leitner, Shlomo E. Blum, Ariel L. Rivas |
| Title | Analysis of raw goat milk microbiota: Impact of stage of lactation and lysozyme on microbial diversity |
| Journal | Food Microbiology. 2014 |
| Authors | Elizabeth A. McInnis, Karen M. Kalanetra, David A. Mills, Elizabeth A. Maga |
| Title | Molecular analysis of spoilage-related bacteria in pasteurized milk during refrigeration by PCR and denaturing gradient gel electrophoresis |
| Journal | Journal of Food Protection. 2009 |
| Authors | He H, Dong J, Lee CN, Li Y |
| Title | Repeat-based subtyping and grouping of Staphylococcus aureus |
| Journal | Diagnostic Microbiology and Infectious Disease. 2009 |
| Authors | Said KB, Ramotar K, Zhu G, Zhao X. |
Milk Bacterial DNA Isolation Kit
This kit provides a rapid spin column method for the isolation and purification of genomic DNA from both Gram-negative and Gram-positive bacteria in milk samples.  It can also be used to process challenging milk samples such as Mastitic milk for example. The kit is highly sensitive and can isolate DNA from a cell density of as little as 10 bacteria contained in 1 mL of milk.  Genomic DNA is isolated in 45 minutes, free of inhibitors and ready for any number of downstream applications including PCR, qPCR and Southern Blot analysis, sequencing and more.​
Supporting Data
Kit Specifications
|
Kit Specifications
|
|
| Maximum Milk Input | 1 mL |
| Time to Complete 10 Purifications | 1 hour |
| DNA Yield* | 500 ng to 8 μg |
| Bacteria Species Processed | Gram positive and Gram negative |
| Minimum Detection Limit | 10 bacteria in 1 mL of milk |
*The range of the DNA yield will vary depending upon a number of factors including bacterial species and type of milk (fat content and %)
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The Lysozyme should be stored at -20°C upon arrival, and the Resuspension Solution A should be stored at -20°C after addition of the lysozyme. The lyophilized Proteinase K should be stored at -20°C upon arrival and after reconstitution. This kit is stable for 1 year from the date of shipment.
| Title | The microbial composition of kefir fermented from raw milk |
| Journal | The microbial composition of kefir fermented from raw milk. 2022 |
| Authors | Luuk van Ooijen, Ton Baars and Remco Kort |
| Title | Bacterial ecology of artisanal Minas cheeses assessed by culture-dependent and -independent methods |
| Journal | Food Microbiology. 2017 |
| Authors | Luana Martins Perin, Maria Luisa Savo Sardaro, LuÃs Augusto Nero, Erasmo Neviani, Monica Gatti |
| Title | Microbiota network and mathematic microbe mutualism in colostrum and mature milk collected in two different geographic areas: Italy versus Burundi |
| Journal | ISME Journal. 2016 |
| Authors | Drago L, Toscano M, De Grandi R, Grossi E, Padovani EM, Peroni DG |
| Title | Treating Generational Stress: Effect of Paternal Stress on Development of Memory and Extinction in Offspring Is Reversed by Probiotic Treatment |
| Journal | Psychological Science. 2016 |
| Authors | Callaghan BL, Cowan CSM, Richardson R |
| Title | Molecular diagnosis of bovine tuberculosis in bovine and human samples: implications for zoonosis |
| Journal | Future Microbiology. 2015 |
| Authors | Md Masudur Rahman, Monira Noor, Kazi Mehetazul Islam, Md Bashir Uddin, Ferdaus Mohd Altaf Hossain, Mohammad Ali Zinnah, Mohammad Al Mamun, Mohammad Rafiqul Islam, Seong Kug Eo & Hossam M Ashour |
| Title | Visualizing the Indefinable: Three-Dimensional Complexity of ‘Infectious Diseases’ |
| Journal | PLOS ONE. 2015 |
| Authors | Gabriel Leitner, Shlomo E. Blum, Ariel L. Rivas |
| Title | Analysis of raw goat milk microbiota: Impact of stage of lactation and lysozyme on microbial diversity |
| Journal | Food Microbiology. 2014 |
| Authors | Elizabeth A. McInnis, Karen M. Kalanetra, David A. Mills, Elizabeth A. Maga |
| Title | Molecular analysis of spoilage-related bacteria in pasteurized milk during refrigeration by PCR and denaturing gradient gel electrophoresis |
| Journal | Journal of Food Protection. 2009 |
| Authors | He H, Dong J, Lee CN, Li Y |
| Title | Repeat-based subtyping and grouping of Staphylococcus aureus |
| Journal | Diagnostic Microbiology and Infectious Disease. 2009 |
| Authors | Said KB, Ramotar K, Zhu G, Zhao X. |
