Milk Bacterial DNA Isolation Kit (Cat. 21550)

Milk Bacterial DNA Isolation Kit

Cat. 21550

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Milk Bacterial DNA Isolation Kit

Cat. 21550

For the rapid purification of genomic DNA from various bacterial species found in milk

Genomic DNA can be isolated from as few as 10 bacterial cells in 1 mL of milk
Isolate genomic DNA from both Gram-negative and Gram-positive bacteria in milk
Can process challenging samples such as mastitic milk
Inhibitor-free DNA is ready for PCR, qPCR, Southern Blot, sequencing & more
Fast and efficient spin-column format

For research use only and NOT intended for in vitro diagnostics.

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Milk Bacterial DNA Isolation Kit
(Cat. 21550) 50 preps

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Kit Size

Milk Bacterial DNA Isolation Kit

This kit provides a rapid spin column method for the isolation and purification of genomic DNA from both Gram-negative and Gram-positive bacteria in milk samples. It can also be used to process challenging milk samples such as Mastitic milk for example. The kit is highly sensitive and can isolate DNA from a cell density of as little as 10 bacteria contained in 1 mL of milk. Genomic DNA is isolated in 45 minutes, free of inhibitors and ready for any number of downstream applications including PCR, qPCR and Southern Blot analysis, sequencing and more.

Kit Specifications

You have selected: Cat. 21550

Kit Specifications
Maximum Milk Input	1 mL
Time to Complete 10 Purifications	1 hour
DNA Yield*	500 ng to 8 μg
Bacteria Species Processed	Gram positive and Gram negative
Minimum Detection Limit	10 bacteria in 1 mL of milk

*The range of the DNA yield will vary depending upon a number of factors including bacterial species and type of milk (fat content and %)

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The Lysozyme should be stored at -20°C upon arrival, and the Resuspension Solution A should be stored at -20°C after addition of the lysozyme. The lyophilized Proteinase K should be stored at -20°C upon arrival and after reconstitution. This kit is stable for 1 year from the date of shipment.

Supporting Data

Figure 1. Isolation and Detection of DNA from as Little as 10 Bacterial Cells in 1 mL of Milk. Increasing amounts of E coli (10¹cells, 10³cells, 10⁵cells, 10⁷cells) were added to 1 mL pasteurized milk samples, and the bacterial DNA was subsequently isolated using Norgen's Milk Bacterial DNA Isolation Kit with a 200 µL elution volume. One microliter of the isolated genomic DNA was then detected in a 20 µL real-time PCR reactionusing primers specific to E coli. Bacterial genomic DNA could be isolated and detected from all the 1 mL milk samples, including the sample that contained only 10 bacterial cells (blue line).

Figure 2. End-Point PCR of Milk Bacterial DNA samples.One milliliter of milk was spiked with various amount of E coli. Bacterial DNA was isolated in duplicate using Norgen's Milk Bacterial DNA Isolation Kit, and 1 µL of each 200 µLelution was used for conventional PCR. For analysis 10 µL of each PCR was loaded on an agarose gel. The amount of bacteria in each milk sample is as follows: A = 10⁷cells, B = 10⁵cells, C = 10³cells, D = 10¹cells. Lane X is a negative control (no template) and Lane M is Norgen's PCRSizer 100bp DNA Ladder.

Figure 3. Hierarchial Clustering Dendrogram of Milk Related Microorganisms Found in Milk Samples.DNA was isolated using Norgens Milk Bacterial DNA Isolation Kit and the 16S Metagenomic Sequencing Library was prepared according to the Illumina MiSeq System. This dendogram shows a hierarchial clustering of samples based on genus-level classifications. The barchart beneath each sample shows the relative abundance of its genus-level classifications.

Click for expanded view

Component	Cat. 21550 (50 preps)
Resuspension Solution A	6 mL
Buffer SK	60 mL
Wash Solution A	18 mL
Elution Buffer B	15 mL
Proteinase K	12 mg
Lysozyme (powder)	120 mg
Spin Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1

Protocols:

(21550) Milk Bacterial DNA Isolation Kit - Protocol (50 prep)

Safety Data Sheets:

(21550) Milk Bacterial DNA Isolation Kit - Safety Data Sheet

Posters:

The Inhibitors of Polymerase Chain Reaction In A Biological Culture - ASCB 2007

Rapid Purification of DNA With High PCR Efficiency From Mastitis Bacteria In Milk Using Silicon Carbide - ASM 2007

Title	The microbial composition of kefir fermented from raw milk
Journal	The microbial composition of kefir fermented from raw milk. 2022
Authors	Luuk van Ooijen, Ton Baars and Remco Kort

Title	Bacterial ecology of artisanal Minas cheeses assessed by culture-dependent and -independent methods
Journal	Food Microbiology. 2017
Authors	Luana Martins Perin, Maria Luisa Savo Sardaro, Luís Augusto Nero, Erasmo Neviani, Monica Gatti

Title	Microbiota network and mathematic microbe mutualism in colostrum and mature milk collected in two different geographic areas: Italy versus Burundi
Journal	ISME Journal. 2016
Authors	Drago L, Toscano M, De Grandi R, Grossi E, Padovani EM, Peroni DG

Title	Treating Generational Stress: Effect of Paternal Stress on Development of Memory and Extinction in Offspring Is Reversed by Probiotic Treatment
Journal	Psychological Science. 2016
Authors	Callaghan BL, Cowan CSM, Richardson R

Title	Molecular diagnosis of bovine tuberculosis in bovine and human samples: implications for zoonosis
Journal	Future Microbiology. 2015
Authors	Md Masudur Rahman, Monira Noor, Kazi Mehetazul Islam, Md Bashir Uddin, Ferdaus Mohd Altaf Hossain, Mohammad Ali Zinnah, Mohammad Al Mamun, Mohammad Rafiqul Islam, Seong Kug Eo & Hossam M Ashour

Title	Visualizing the Indefinable: Three-Dimensional Complexity of ‘Infectious Diseases’
Journal	PLOS ONE. 2015
Authors	Gabriel Leitner, Shlomo E. Blum, Ariel L. Rivas

Title	Analysis of raw goat milk microbiota: Impact of stage of lactation and lysozyme on microbial diversity
Journal	Food Microbiology. 2014
Authors	Elizabeth A. McInnis, Karen M. Kalanetra, David A. Mills, Elizabeth A. Maga

Title	Molecular analysis of spoilage-related bacteria in pasteurized milk during refrigeration by PCR and denaturing gradient gel electrophoresis
Journal	Journal of Food Protection. 2009
Authors	He H, Dong J, Lee CN, Li Y

Title	Repeat-based subtyping and grouping of Staphylococcus aureus
Journal	Diagnostic Microbiology and Infectious Disease. 2009
Authors	Said KB, Ramotar K, Zhu G, Zhao X.

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Milk Bacterial DNA Isolation Kit

This kit provides a rapid spin column method for the isolation and purification of genomic DNA from both Gram-negative and Gram-positive bacteria in milk samples. It can also be used to process challenging milk samples such as Mastitic milk for example. The kit is highly sensitive and can isolate DNA from a cell density of as little as 10 bacteria contained in 1 mL of milk. Genomic DNA is isolated in 45 minutes, free of inhibitors and ready for any number of downstream applications including PCR, qPCR and Southern Blot analysis, sequencing and more.

Supporting Data

Figure 1. Isolation and Detection of DNA from as Little as 10 Bacterial Cells in 1 mL of Milk. Increasing amounts of E coli (10¹cells, 10³cells, 10⁵cells, 10⁷cells) were added to 1 mL pasteurized milk samples, and the bacterial DNA was subsequently isolated using Norgen's Milk Bacterial DNA Isolation Kit with a 200 µL elution volume. One microliter of the isolated genomic DNA was then detected in a 20 µL real-time PCR reactionusing primers specific to E coli. Bacterial genomic DNA could be isolated and detected from all the 1 mL milk samples, including the sample that contained only 10 bacterial cells (blue line).

Figure 2. End-Point PCR of Milk Bacterial DNA samples.One milliliter of milk was spiked with various amount of E coli. Bacterial DNA was isolated in duplicate using Norgen's Milk Bacterial DNA Isolation Kit, and 1 µL of each 200 µLelution was used for conventional PCR. For analysis 10 µL of each PCR was loaded on an agarose gel. The amount of bacteria in each milk sample is as follows: A = 10⁷cells, B = 10⁵cells, C = 10³cells, D = 10¹cells. Lane X is a negative control (no template) and Lane M is Norgen's PCRSizer 100bp DNA Ladder.

Figure 3. Hierarchial Clustering Dendrogram of Milk Related Microorganisms Found in Milk Samples.DNA was isolated using Norgens Milk Bacterial DNA Isolation Kit and the 16S Metagenomic Sequencing Library was prepared according to the Illumina MiSeq System. This dendogram shows a hierarchial clustering of samples based on genus-level classifications. The barchart beneath each sample shows the relative abundance of its genus-level classifications.

Click for expanded view

Kit Specifications

You have selected: Cat. 21550

Kit Specifications
Maximum Milk Input	1 mL
Time to Complete 10 Purifications	1 hour
DNA Yield*	500 ng to 8 μg
Bacteria Species Processed	Gram positive and Gram negative
Minimum Detection Limit	10 bacteria in 1 mL of milk

*The range of the DNA yield will vary depending upon a number of factors including bacterial species and type of milk (fat content and %)

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The Lysozyme should be stored at -20°C upon arrival, and the Resuspension Solution A should be stored at -20°C after addition of the lysozyme. The lyophilized Proteinase K should be stored at -20°C upon arrival and after reconstitution. This kit is stable for 1 year from the date of shipment.

Component	Cat. 21550 (50 preps)
Resuspension Solution A	6 mL
Buffer SK	60 mL
Wash Solution A	18 mL
Elution Buffer B	15 mL
Proteinase K	12 mg
Lysozyme (powder)	120 mg
Spin Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1

Protocols:

(21550) Milk Bacterial DNA Isolation Kit - Protocol (50 prep)

Safety Data Sheets:

(21550) Milk Bacterial DNA Isolation Kit - Safety Data Sheet

Posters:

The Inhibitors of Polymerase Chain Reaction In A Biological Culture - ASCB 2007

Rapid Purification of DNA With High PCR Efficiency From Mastitis Bacteria In Milk Using Silicon Carbide - ASM 2007

Title	The microbial composition of kefir fermented from raw milk
Journal	The microbial composition of kefir fermented from raw milk. 2022
Authors	Luuk van Ooijen, Ton Baars and Remco Kort

Title	Bacterial ecology of artisanal Minas cheeses assessed by culture-dependent and -independent methods
Journal	Food Microbiology. 2017
Authors	Luana Martins Perin, Maria Luisa Savo Sardaro, Luís Augusto Nero, Erasmo Neviani, Monica Gatti

Title	Microbiota network and mathematic microbe mutualism in colostrum and mature milk collected in two different geographic areas: Italy versus Burundi
Journal	ISME Journal. 2016
Authors	Drago L, Toscano M, De Grandi R, Grossi E, Padovani EM, Peroni DG

Title	Treating Generational Stress: Effect of Paternal Stress on Development of Memory and Extinction in Offspring Is Reversed by Probiotic Treatment
Journal	Psychological Science. 2016
Authors	Callaghan BL, Cowan CSM, Richardson R

Title	Molecular diagnosis of bovine tuberculosis in bovine and human samples: implications for zoonosis
Journal	Future Microbiology. 2015
Authors	Md Masudur Rahman, Monira Noor, Kazi Mehetazul Islam, Md Bashir Uddin, Ferdaus Mohd Altaf Hossain, Mohammad Ali Zinnah, Mohammad Al Mamun, Mohammad Rafiqul Islam, Seong Kug Eo & Hossam M Ashour

Title	Visualizing the Indefinable: Three-Dimensional Complexity of ‘Infectious Diseases’
Journal	PLOS ONE. 2015
Authors	Gabriel Leitner, Shlomo E. Blum, Ariel L. Rivas

Title	Analysis of raw goat milk microbiota: Impact of stage of lactation and lysozyme on microbial diversity
Journal	Food Microbiology. 2014
Authors	Elizabeth A. McInnis, Karen M. Kalanetra, David A. Mills, Elizabeth A. Maga

Title	Molecular analysis of spoilage-related bacteria in pasteurized milk during refrigeration by PCR and denaturing gradient gel electrophoresis
Journal	Journal of Food Protection. 2009
Authors	He H, Dong J, Lee CN, Li Y

Title	Repeat-based subtyping and grouping of Staphylococcus aureus
Journal	Diagnostic Microbiology and Infectious Disease. 2009
Authors	Said KB, Ramotar K, Zhu G, Zhao X.

At Norgen Biotek Corp. we are doing our part in response to the global coronavirus SARS-CoV-2 outbreak. | Find out how

Milk Bacterial DNA Isolation Kit (Cat. 21550)

Milk Bacterial DNA Isolation Kit

Milk Bacterial DNA Isolation Kit

Kit Size

Related Products

Milk DNA Preservation and Isolation Kit

Bacterial Genomic DNA Isolation Kit

Fungi/Yeast Genomic DNA Isolation Kits

At Norgen Biotek Corp. we are doing our part in response to the global coronavirus SARS-CoV-2 outbreak. | Find out how

Milk Bacterial DNA Isolation Kit (Cat. 21550)

Milk Bacterial DNA Isolation Kit

Milk Bacterial DNA Isolation Kit

Kit Size

Product Overview

Documentation

Citations

Related Products

Milk DNA Preservation and Isolation Kit

Bacterial Genomic DNA Isolation Kit

Fungi/Yeast Genomic DNA Isolation Kits