Milk Bacterial DNA Isolation Kit For the rapid purification of genomic DNA from various bacterial species found in milk For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Milk Bacterial DNA Isolation Kit For the rapid purification of genomic DNA from various bacterial species found in milk Price USD$195.00 SKU 21550 Format Spin Column Size 50 Preps Quantity Overview Details Documentation Citations Overview Genomic DNA can be isolated from as few as 10 bacterial cells in 1 mL of milk Isolate genomic DNA from both Gram-negative and Gram-positive bacteria in milk Can process challenging samples such as mastitic milk Inhibitor-free DNA is ready for PCR, qPCR, Southern Blot, sequencing & more Fast and efficient spin-column format This kit provides a rapid spin column method for the isolation and purification of genomic DNA from both Gram-negative and Gram-positive bacteria in milk samples. It can also be used to process challenging milk samples such as Mastitic milk for example. The kit is highly sensitive and can isolate DNA from a cell density of as little as 10 bacteria contained in 1 mL of milk. Genomic DNA is isolated in 45 minutes, free of inhibitors and ready for any number of downstream applications including PCR, qPCR and Southern Blot analysis, sequencing and more. Details Supporting Data Previous Figure 1. Isolation and Detection of DNA from as Little as 10 Bacterial Cells in 1 mL of Milk. Increasing amounts of E coli (101 cells, 103 cells, 105 cells, 107 cells) were added to 1 mL pasteurized milk samples, and the bacterial DNA was subsequently isolated using Norgen's Milk Bacterial DNA Isolation Kit with a 200 µL elution volume. One microliter of the isolated genomic DNA was then detected in a 20 µL real-time PCR reaction using primers specific to E coli. Bacterial genomic DNA could be isolated and detected from all the 1 mL milk samples, including the sample that contained only 10 bacterial cells (blue line). Figure 2. End-Point PCR of Milk Bacterial DNA samples. One milliliter of milk was spiked with various amount of E coli. Bacterial DNA was isolated in duplicate using Norgen's Milk Bacterial DNA Isolation Kit, and 1 µL of each 200 µL elution was used for conventional PCR. For analysis 10 µL of each PCR was loaded on an agarose gel. The amount of bacteria in each milk sample is as follows: A = 107 cells, B = 105 cells, C = 103 cells, D = 101 cells. Lane X is a negative control (no template) and Lane M is Norgen's PCRSizer 100bp DNA Ladder. Figure 3. Hierarchial Clustering Dendrogram of Milk Related Microorganisms Found in Milk Samples. DNA was isolated using Norgen’s Milk Bacterial DNA Isolation Kit and the 16S Metagenomic Sequencing Library was prepared according to the Illumina MiSeq System. This dendogram shows a hierarchial clustering of samples based on genus-level classifications. The barchart beneath each sample shows the relative abundance of its genus-level classifications. Next Click for expanded view Kit Specifications Maximum Milk Input 1 mL Time to Complete 10 Purifications 1 hour DNA Yield* 500 ng to 8 μg Bacteria Species Processed Gram positive and Gram negative Minimum Detection Limit 10 bacteria in 1 mL of milk *The range of the DNA yield will vary depending upon a number of factors including bacterial species and type of milk (fat content and %) Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. The Lysozyme should be stored at -20°C upon arrival, and the Resuspension Solution A should be stored at -20°C after addition of the lysozyme. The lyophilized Proteinase K should be stored at -20°C upon arrival and after reconstitution. This kit is stable for 1 year from the date of shipment. Component Cat. 21550 (50 preps) Resuspension Solution A 6 mL Buffer SK 60 mL Wash Solution A 18 mL Elution Buffer B 15 mL Proteinase K 12 mg Lysozyme (powder) 120 mg Spin Columns 50 Collection Tubes 50 Elution Tubes (1.7 mL) 50 Product Insert 1 Documentation PROTOCOLS (21550) Milk Bacterial DNA Isolation Kit - Protocol (50 prep) SAFETY DATA SHEETS (21550) Milk Bacterial DNA Isolation Kit - Safety Data Sheet POSTERS The Inhibitors of Polymerase Chain Reaction In A Biological Culture - ASCB 2007 Rapid Purification of DNA With High PCR Efficiency From Mastitis Bacteria In Milk Using Silicon Carbide - ASM 2007 Citations Title The microbial composition of kefir fermented from raw milk Journal The microbial composition of kefir fermented from raw milk. 2022 Authors Luuk van Ooijen, Ton Baars and Remco Kort Title Bacterial ecology of artisanal Minas cheeses assessed by culture-dependent and -independent methods Journal Food Microbiology. 2017 Authors Luana Martins Perin, Maria Luisa Savo Sardaro, Luís Augusto Nero, Erasmo Neviani, Monica Gatti Title Microbiota network and mathematic microbe mutualism in colostrum and mature milk collected in two different geographic areas: Italy versus Burundi Journal ISME Journal. 2016 Authors Drago L, Toscano M, De Grandi R, Grossi E, Padovani EM, Peroni DG Title Treating Generational Stress: Effect of Paternal Stress on Development of Memory and Extinction in Offspring Is Reversed by Probiotic Treatment Journal Psychological Science. 2016 Authors Callaghan BL, Cowan CSM, Richardson R Title Molecular diagnosis of bovine tuberculosis in bovine and human samples: implications for zoonosis Journal Future Microbiology. 2015 Authors Md Masudur Rahman, Monira Noor, Kazi Mehetazul Islam, Md Bashir Uddin, Ferdaus Mohd Altaf Hossain, Mohammad Ali Zinnah, Mohammad Al Mamun, Mohammad Rafiqul Islam, Seong Kug Eo & Hossam M Ashour Title Visualizing the Indefinable: Three-Dimensional Complexity of ‘Infectious Diseases’ Journal PLOS ONE. 2015 Authors Gabriel Leitner, Shlomo E. Blum, Ariel L. Rivas Title Analysis of raw goat milk microbiota: Impact of stage of lactation and lysozyme on microbial diversity Journal Food Microbiology. 2014 Authors Elizabeth A. McInnis, Karen M. Kalanetra, David A. Mills, Elizabeth A. Maga Title Molecular analysis of spoilage-related bacteria in pasteurized milk during refrigeration by PCR and denaturing gradient gel electrophoresis Journal Journal of Food Protection. 2009 Authors He H, Dong J, Lee CN, Li Y Title Repeat-based subtyping and grouping of Staphylococcus aureus Journal Diagnostic Microbiology and Infectious Disease. 2009 Authors Said KB, Ramotar K, Zhu G, Zhao X. Related Products Milk DNA Preservation and Isolation Kit Bacterial Genomic DNA Isolation Kit Fungi/Yeast Genomic DNA Isolation Kit
Milk Bacterial DNA Isolation Kit For the rapid purification of genomic DNA from various bacterial species found in milk For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Milk Bacterial DNA Isolation Kit For the rapid purification of genomic DNA from various bacterial species found in milk Price USD$195.00 SKU 21550 Format Spin Column Size 50 Preps Quantity
