Ideal for quick assessment of PCR products

  • Ready-to-use
  • Quantitative
  • Highly Stable
  • Precise
  • Fourteen discrete fragments ranging from 25 bp to 650 bp in 50 bp increments
  • Double intensity reference bands at 200 bp and 400 bp

ItemsCat. #Size
Mini Sizer 50 bp DNA Ladder 11200 100 loads

MiniSizer 50 bp DNA Ladder

The Norgen MiniSizer 50 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. This Ladder contains fourteen discrete fragments ranging from 25 bp to 650 bp in 50 bp increments with double intensity reference bands at 200 bp and 400 bp. The MiniSizer is ideal for PCR product size confirmation.

Contents:

1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

MiniSizer 50 bp DNA Ladder (Cat# 11200) - 100 loads

Ladder Properties:
• Fourteen discrete bands, ranging from 50 bp to 650 bp
• Higher intensity bands at 200 bp and 400 bp for easy reference

 

Fragment
Size (bp)
Mass (ng)
1
650
61
2
600
46
3
550
36
4
500
30
5
450
35
6
400
85
7
350
27
8
300
25
9
250
16
10
200
54
11
150
33 
12 100 21
13 50 17
14 25 14
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
 
Storage: 
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.

 

Title Association of NOD1, CXCL16, STAT6 and TLR4 gene polymorphisms with Malaysian patients with Crohn’s disease
Journal PeerJ. 2016.
Authors Chua KH, Ng JG, Ng CC, Hilmi I, Goh KL, Kee BP
Title Gamma radiation synthesis optimization of a novel polymer and its possible applications in antimicrobial and antitumor fields.
Journal Medicinal Chemistry Research. 2012.
Authors Mostafa T, Awadallah-F A and Shehata M.