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Norovirus is a single-stranded, non-enveloped RNA virus belonging to the Caliciviridae family. Norovirus is considered the major causative agent of non-bacterial gastroenteritis in the world. The main channel of transmittance is via contaminated food or water, as well as human-to-human contact. Most Noroviruses infecting humans belong to genogroup I and II (particularly genotype 4). Norovirus is very stable in the environment and is resistant to some surface disinfectants such as alcohols and detergents. Moreover, it is very difficult to culture Norovirus in vitro, making its identification by standard microbiological assays challenging.

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Supporting Data

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TaqMan PCR Kit - Figure 1. Example of Norovirus Positive. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR.

TaqMan PCR Kit - Figure 2. Example of Norovirus negative. No target RNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR.

TaqMan PCR Kit - Figure 3. Example of PCR inhibition. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for RNA purification.

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