Norovirus (NoV) TaqMan RT-PCR Detection Kit Dx Intended for in vitro diagnostics use.CE marked version is not available for sale in the United States and Canada Powered by Bioz Norovirus (NoV) TaqMan RT-PCR Detection Kit Dx SKU DxTM41400 Format RT-PCR Size 24 Reactions Contact Distributor Overview Details Documentation Citations Overview Detection kits for NoV CE-IVDD marked in accordance with the European Commission Directive 98/79/EC. Research version available Available in TaqMan format for analysis Norovirus is a single-stranded, non-enveloped RNA virus belonging to the Caliciviridae family. Norovirus is considered the major causative agent of non-bacterial gastroenteritis in the world. The main channel of transmittance is via contaminated food or water, as well as human-to-human contact. Most Noroviruses infecting humans belong to genogroup I and II (particularly genotype 4). Norovirus is very stable in the environment and is resistant to some surface disinfectants such as alcohols and detergents. Moreover, it is very difficult to culture Norovirus in vitro, making its identification by standard microbiological assays challenging. TaqMan PCR Kit Click to expand options NoV TaqMan RT-PCR Kit, 24 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents Details Supporting Data Previous TaqMan PCR Kit - Figure 1. Example of Norovirus Positive. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 2. Example of Norovirus negative. No target RNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR. TaqMan PCR Kit - Figure 3. Example of PCR inhibition. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for RNA purification. Next Click for expanded view Storage Conditions The NoV TaqMan RT-PCR Kit Dx is shipped on dry ice. The components of the kit should be frozen upon arrival. If one or more of the components is not frozen when the kit is received, or if any of the components have been compromised during shipment, please contact Norgen Biotek for assistance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 3 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. Component Cat. DxTM41400 (24 rxns) MDx TaqMan 2X RT-PCR Master Mix Dx 550 µL NoV Primer & Probe Mix Dx 2 x 70 µL NoV Positive Control Dx - 200,000 copies/μL 50 µL Nuclease-Free Water 2 x 1.25 mL Product Insert 1 Documentation PROTOCOLS (DxTM41400) NoV TaqMan RT-PCR Kit Dx - Protocol (24 rxns) SAFETY DATA SHEETS (DxTM41400) NoV TaqMan RT-PCR Kit Dx - Safety Data Sheet Citations Title Use of dual priming oligonucleotide system-based multiplex RT-PCR combined with high performance liquid chromatography assay for simultaneous detection of five enteric viruses associated with acute enteritis Journal Journal of Virological Methods. 2017. Authors Fan WL, Wang ZW, Qin Y, Sun C, Liu ZM, Jiang YP, Qiao XY, Tang LJ, Li YJ, Xu YG. Related Products Human Immunodeficiency Virus (HIV) TaqMan Probe/Primer and Control Set Plasma/Serum RNA Purification Kit
Norovirus (NoV) TaqMan RT-PCR Detection Kit Dx Intended for in vitro diagnostics use.CE marked version is not available for sale in the United States and Canada Powered by Bioz Norovirus (NoV) TaqMan RT-PCR Detection Kit Dx SKU DxTM41400 Format RT-PCR Size 24 Reactions Contact Distributor
