Fast and simple clean-up and concentration of oligonucleotides without the use of phenol
|Oligo Clean-Up and Concentration Kit||34100||50 preps|
Oligo Clean-Up and Concentration Kit
This kit provides a rapid spin column procedure for the purification and concentration of up to 10 µg of oligonucleotides of various sizes. The kit is used for the purification of synthesized oligos, as well as the clean-up of oligos from various upstream enzymatic reactions such as ligation, Poly(A) tailing and end-labeling.
Single or double stranded DNA or RNA oligos larger than 10 bp can be purified with this kit (not recommended for the removal of PCR primers). Oligos of up to 1000 bp have been tested with this kit.
This kit purifies oligos from other reaction components including proteins, buffers and nucleotides without the use of phenol, chloroform, alcohol precipitation or urea PAGE extraction.
The kit provides a high quality product with up to 90% recovery. The purified oligos can be used in a number of downstream applications including end-point or quantitative reverse transcription PCR, Northern blotting, in situ hybridization, and RNAi studies.
|Column Binding Capacity||
10 μg for DNA or RNA
|Maximum Column Loading Volume||
|Size of DNA/RNA Purified||
> 10 nt, single stranded
or double stranded
|Maximum Amount of Starting Material||
10 μg of DNA or RNA
|Minimum Elution Volume||
|Time to Complete 10 Purifications||
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
|Title||Mapping the λ Integrase bridges in the nucleoprotein Holliday junction intermediates of viral integrative and excisive recombination.|
|Journal||Proceedings of the National Academy of Sciences of the USA .. 2014.|
|Authors||Wenjun Tong, David Warren, Nicole E. Seah, Gurunathan Laxmikanthan, Gregory D. Van Duyne, and Arthur Landy.|
|Title||Nucleoprotein architectures regulating the directionality of viral integration and excision.|
|Journal||Proceedings of the National Academy of Sciences of the USA 2014.|
|Authors||Seah NE, Warren D, Tong W, Laxmikanthan G, Van Duyne GD, Landy A.|
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