Oligo Clean-Up and Concentration Kit (Cat. 34100)
Oligo Clean-Up and Concentration Kit
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Oligo Clean-Up and Concentration Kit
Fast and simple clean-up and concentration of oligonucleotides without the use of phenol
- Cleans and concentrates single-stranded or double-stranded DNA or RNA oligonucleotides larger than 10 bases
- Rapid and efficient spin column procedure
- No phenol, chloroform or alcohol precipitations are involved
- High recovery of up to 90%
- Efficient removal of enzymatic reaction buffers and proteins
- Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
For research use only and NOT intended for in vitro diagnostics.
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(Cat. 34100) 50 preps
Product Format Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.
Product Size
Kit Specifications
|
Kit Specifications
|
|
| Column Binding Capacity |
10 μg for DNA or RNA
|
| Maximum Column Loading Volume |
600 μL
|
| Size of DNA/RNA Purified |
> 10 nt, single stranded
or double stranded |
| Maximum Amount of Starting Material |
10 μg of DNA or RNA
|
| Minimum Elution Volume |
20 μL
|
| Time to Complete 10 Purifications |
15 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment.
Supporting Data
| Component | Cat. 34100 (50 preps) |
|---|---|
| Buffer RL | 30 mL |
| Wash Solution A | 20 mL |
| Elution Solution A | 6 mL |
| Micro Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
| Title | N1-methyl-pseudouridine is incorporated with higher fidelity than pseudouridine in synthetic RNAs |
| Journal | Scientific Reports. 2022 |
| Authors | Tien-Hao Chen, Vladimir Potapov, Nan Dai, Jennifer L. Ong & Bijoyita Roy |
| Title | A Novel Rolling Circle Amplification-Based Detection of SARS-CoV-2 with Multi-Region Padlock Hybridization |
| Journal | Diagnostics. 2022 |
| Authors | Rajesh Kumari, Ji Won Lim, Matthew Ryan Sullivan, Rachel Malampy, Connor Baush, Irina Smolina, Howard Robin, Vadim V. Demidov, Giovanni Stefano Ugolini, Jared R. Auclair and Tania Konry |
| Title | RNA binding to human METTL3-METTL14 restricts N6-deoxyadenosine methylation of DNA in vitro |
| Journal | Biochemistry and Chemical Biology. 2022 |
| Authors | Shan Qi, Javier Mota, Siu-Hong Chan, Johanna Villarreal, Nan Dai, Shailee Arya, Robert A Hromas, Manjeet K Rao, Ivan R Corrêa Jr, et al. |
| Title | Carbamoyltransferase Enzyme Assay: In vitro Modification of 5-hydroxymethylcytosine (5hmC) to 5-carbamoyloxymethylcytosine (5cmC) |
| Journal | bio-protocol. 2022 |
| Authors | Weiwei Yang, Nan Dai, Yu-Cheng Lin, William Johnson, Romualdas Vaisvila, Peter Weigele, Yan-Jiun Lee, Ira Schildkraut, Ivan R. Correa Jr, Laurence Ettwiller |
| Title | Mapping the λ Integrase bridges in the nucleoprotein Holliday junction intermediates of viral integrative and excisive recombination. |
| Journal | Proceedings of the National Academy of Sciences of the USA .. 2014. |
| Authors | Wenjun Tong, David Warren, Nicole E. Seah, Gurunathan Laxmikanthan, Gregory D. Van Duyne, and Arthur Landy. |
| Title | Nucleoprotein architectures regulating the directionality of viral integration and excision. |
| Journal | Proceedings of the National Academy of Sciences of the USA 2014. |
| Authors | Seah NE, Warren D, Tong W, Laxmikanthan G, Van Duyne GD, Landy A. |
Oligo Clean-Up and Concentration Kit
This kit provides a rapid spin column procedure for the purification and concentration of up to 10 µg of oligonucleotides of various sizes. The kit is used for the purification of synthesized oligos, as well as the clean-up of oligos from various upstream enzymatic reactions such as ligation, Poly(A) tailing and end-labeling.
Single or double stranded DNA or RNA oligos larger than 10 bp can be purified with this kit (not recommended for the removal of PCR primers). Oligos of up to 1000 bp have been tested with this kit.
This kit purifies oligos from other reaction components including proteins, buffers and nucleotides without the use of phenol, chloroform, alcohol precipitation or urea PAGE extraction.
The kit provides a high quality product with up to 90% recovery. The purified oligos can be used in a number of downstream applications including end-point or quantitative reverse transcription PCR, Northern blotting, in situ hybridization, and RNAi studies.
Supporting Data
Kit Specifications
|
Kit Specifications
|
|
| Column Binding Capacity |
10 μg for DNA or RNA
|
| Maximum Column Loading Volume |
600 μL
|
| Size of DNA/RNA Purified |
> 10 nt, single stranded
or double stranded |
| Maximum Amount of Starting Material |
10 μg of DNA or RNA
|
| Minimum Elution Volume |
20 μL
|
| Time to Complete 10 Purifications |
15 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment.
| Component | Cat. 34100 (50 preps) |
|---|---|
| Buffer RL | 30 mL |
| Wash Solution A | 20 mL |
| Elution Solution A | 6 mL |
| Micro Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
| Title | N1-methyl-pseudouridine is incorporated with higher fidelity than pseudouridine in synthetic RNAs |
| Journal | Scientific Reports. 2022 |
| Authors | Tien-Hao Chen, Vladimir Potapov, Nan Dai, Jennifer L. Ong & Bijoyita Roy |
| Title | A Novel Rolling Circle Amplification-Based Detection of SARS-CoV-2 with Multi-Region Padlock Hybridization |
| Journal | Diagnostics. 2022 |
| Authors | Rajesh Kumari, Ji Won Lim, Matthew Ryan Sullivan, Rachel Malampy, Connor Baush, Irina Smolina, Howard Robin, Vadim V. Demidov, Giovanni Stefano Ugolini, Jared R. Auclair and Tania Konry |
| Title | RNA binding to human METTL3-METTL14 restricts N6-deoxyadenosine methylation of DNA in vitro |
| Journal | Biochemistry and Chemical Biology. 2022 |
| Authors | Shan Qi, Javier Mota, Siu-Hong Chan, Johanna Villarreal, Nan Dai, Shailee Arya, Robert A Hromas, Manjeet K Rao, Ivan R Corrêa Jr, et al. |
| Title | Carbamoyltransferase Enzyme Assay: In vitro Modification of 5-hydroxymethylcytosine (5hmC) to 5-carbamoyloxymethylcytosine (5cmC) |
| Journal | bio-protocol. 2022 |
| Authors | Weiwei Yang, Nan Dai, Yu-Cheng Lin, William Johnson, Romualdas Vaisvila, Peter Weigele, Yan-Jiun Lee, Ira Schildkraut, Ivan R. Correa Jr, Laurence Ettwiller |
| Title | Mapping the λ Integrase bridges in the nucleoprotein Holliday junction intermediates of viral integrative and excisive recombination. |
| Journal | Proceedings of the National Academy of Sciences of the USA .. 2014. |
| Authors | Wenjun Tong, David Warren, Nicole E. Seah, Gurunathan Laxmikanthan, Gregory D. Van Duyne, and Arthur Landy. |
| Title | Nucleoprotein architectures regulating the directionality of viral integration and excision. |
| Journal | Proceedings of the National Academy of Sciences of the USA 2014. |
| Authors | Seah NE, Warren D, Tong W, Laxmikanthan G, Van Duyne GD, Landy A. |
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