Parvovirus B19 TaqMan PCR Detection Kit Dx Intended for in vitro diagnostics use.CE marked version is not available for sale in the United States and Canada Powered by Bioz Parvovirus B19 TaqMan PCR Detection Kit Dx Price USD$325.00 SKU DxTM39600 Format RT-PCR Size 24 Reactions Quantity Overview Details Documentation Citations Overview Detection kits for the Parvo B19 CE-IVDD marked in accordance with the European Commission Directive 98/79/EC. Research version available Available in TaqMan format for analysis Parvovirus B19 was the first known human virus in the family of Parvoviruses. B19 is a non-enveloped, icosahedral virus that contains a single-stranded linear DNA genome. It is classified as an erythrovirus because of its capability to invade red blood cell precursors in the bone marrow. B19 virus causes a childhood rash called fifth disease or erythema infectiosum which is commonly called “slapped cheek” syndrome due to the appearance of erythema across the cheeks. The virus is primarily spread by infected respiratory droplets, but some cases of blood-borne transmission have been reported. Parvovirus infection in pregnant women is associated with hydrops fetalis due to severe fetal anemia, sometimes leading to miscarriage or stillbirth. The risk of fetal loss is about 10% if infection occurs before week 20 of pregnancy. There is also some evidence that intrauterine Parvovirus B19 infection leads to developmental abnormalities in childhood. Due to the serious nature of the virus, methods for its rapid diagnosis are desirable. TaqMan PCR Kit Click to expand options Parvo B19 TaqMan PCR Kit, 24 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents Details Supporting Data Previous TaqMan PCR Kit - Figure 1. Example of TaqMan PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 2. Example of TaqMan PCR Negative result. No target DNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR. TaqMan PCR Kit - Figure 3. Example of TaqMan PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification. Next Click for expanded view Storage Conditions The Parvo B19 TaqMan PCR Kit Dx is shipped on dry ice. The components of the kit should be frozen upon arrival. If one or more of the components is not frozen when the kit is received, or if any of the components have been compromised during shipment, please contact Norgen Biotek for assistance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 3 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. Component Cat. DxTM39600 (24 rxns) MDx TaqMan 2X PCR Master Mix Dx 550 µL Parvo B19 Primer & Probe Mix Dx 2 x 70 µL Parvo B19 Positive Control Dx - 200,000 copies/μL 50 µL Nuclease-Free Water 2 x 1.25 mL Product Insert 1 Documentation PROTOCOLS (DxTM39600) Parvo B19 TaqMan PCR Kit Dx - Protocol (24 rxns) SAFETY DATA SHEETS (DxTM39600) Parvo B19 TaqMan PCR Kit Dx - Safety Data Sheet Citations Powered by Bioz See more details on Bioz Related Products Parvo B19 TaqMan Probe/Primer and Control Set Plasma/Serum Cell-Free Circulating DNA Purification Micro Kit
Parvovirus B19 TaqMan PCR Detection Kit Dx Intended for in vitro diagnostics use.CE marked version is not available for sale in the United States and Canada Powered by Bioz Parvovirus B19 TaqMan PCR Detection Kit Dx Price USD$325.00 SKU DxTM39600 Format RT-PCR Size 24 Reactions Quantity