PCR Purification Kit (Cat. 14400, 24800, 45700)
PCR Purification Kit
![PCR Purification Kit](https://norgenbiotek.com/sites/default/files/styles/original/public/images/product_images/14400-PCR-Purification-Kit-WEB.jpg?itok=tNhc78J8" "PCR Purification Kit")
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PCR Purification Kit
For the rapid purification of amplified DNA products from PCR mixes
- Purify amplified DNA ranging from 100 bp -15,000 bp in size
- Fast and efficient spin column format
- Available in a 50 prep size and a 250 prep size
- Also available in 96 well format
For the rapid purification of amplified DNA products from PCR mixes
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(Cat. 14400) 50 preps
(Cat. 24800) 2 x 96-well plate
(Cat. 45700) 250 preps
Product Format Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.
Product Size
Kit Specifications
|
Kit Specifications - Spin Column
|
|
| Column Binding Capacity |
10 μg
|
| Size of DNA Purified |
100 - 15,000 bp
|
| Average Recovery |
> 90%
|
| Average Primer Removal |
> 90%
|
| Minimum Elution Volume |
30 μL
|
| Time to Complete 10 Purifications |
15 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
|
Kit Specifications - 96-well
|
|
| Binding Capacity Per Well |
15 μg
|
| Maximum Loading Volume Per Well |
500 μL
|
| Size of DNA Purified |
100 - 15,000 bp
|
| Average DNA Recovery |
> 90%
|
| Average Primer Reoval |
> 90%
|
| Minimum Elution Volume |
50 μL
|
| Time to Complete 96 Purifications |
20 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
|
Kit Specifications - Spin Column
|
|
| Column Binding Capacity |
10 μg
|
| Size of DNA Purified |
100 - 15,000 bp
|
| Average Recovery |
> 90%
|
| Average Primer Removal |
> 90%
|
| Minimum Elution Volume |
30 μL
|
| Time to Complete 10 Purifications |
15 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
Supporting Data
| Title | Molecular Confirmation, Identification and Influence of Carbon Source for the Production of Xylanase from Penicillium Citrinum |
| Journal | Journal of Drug Delivery and Therapeutics. 2015. |
| Authors | Naveen M and Siddalingeshwara K G |
| Title | Screening and Identification of Ligninolytic Bacteria for the Treatment of Pulp and Paper Mill Effluent |
| Journal | Water, Air, & Soil Pollution. 2015. |
| Authors | Rajni Hooda, Nishi K. Bhardwaj, Pamela Singh |
| Title | AtROS1 overexpression provides evidence for epigenetic regulation of genes encoding enzymes of flavonoid biosynthesis and antioxidant pathways during salt stress in transgenic tobacco |
| Journal | Journal of Experimental Biotechnology. 2015. |
| Authors | Poonam Bharti, Monika Mahajan, Ajay K. Vishwakarma, Jyoti Bhardwaj and Sudesh Kumar Yadav |
| Title | Recent characterization of cowpea aphid-borne mosaic virus (CABMV) in Bahia State, Brazil, suggests potential regional isolation |
| Journal | African Journal of Biotechnology. 2015. |
| Authors | Jose Romario Fernandes de Melo, Antonia dos Reis Figueira, Charles Neris Moreira, Antonio Carlos de Oliveira. |
| Title | Isolation of Bacteria from Soil sample for ExoPolysaccharide production |
| Journal | International Journal of ChemTech Research. 2014. |
| Authors | A. Gowsalya, V. Ponnusami and K.R. Sugumaran. |
| Title | Isolation and Molecular Identification of Extreme an Halotolerant Staphylococcus Sp. 13cc from Brackish Water of Chilika Lake. |
| Journal | Biosciences Biotechnology Research Asia. 2014. |
| Authors | P.D. Mahapatra and B.B. Mishra. |
| Title | Simultaneous cellulose hydrolysis and bio-electricity generation in a mediatorless Microbial Fuel Cell using a Bacillus flexus strain isolated from wastewater. |
| Journal | Research In Biotechnology. 2014. |
| Authors | Shankar N, Panchapakesan A, Bhandari S, Ravishankar H. |
| Title | TAS2R38 single nucleotide polymorphisms are associated with PROP- but not thermal tasting: a pilot study. |
| Journal | Chemosensory Perception. 2013. |
| Authors | Bering A, Pickering G, Liang P. |
| Title | ”First Generation” Automated DNA Sequencing Technology. |
| Journal | Automated DNA Sequencing Technology. Current Protocols in Molecular Biology. 2011. |
| Authors | Slatko B, Kieleczawa J, Ju J, Gardner A, Hendrickson C, and Ausubel F. |
PCR Purification Kit
This kit enables the rapid purification of amplified DNA products from PCR mixes. It is able to effectively remove PCR by-products including primers, dimers, enzymes, unincorporated nucleotides and mineral oil from the desired PCR product. The purified PCR products are fully compatible with restriction enzyme digestion, ligation into vectors, labeling, sequencing and more. This kit can also be used as an alternative to organic extraction and ethanol precipitation to clean up various enzymatic reactions.
The kit is also available in a 96-well format for high-throughput PCR purification. Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation.
Supporting Data
Kit Specifications
|
Kit Specifications - Spin Column
|
|
| Column Binding Capacity |
10 μg
|
| Size of DNA Purified |
100 - 15,000 bp
|
| Average Recovery |
> 90%
|
| Average Primer Removal |
> 90%
|
| Minimum Elution Volume |
30 μL
|
| Time to Complete 10 Purifications |
15 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
|
Kit Specifications - 96-well
|
|
| Binding Capacity Per Well |
15 μg
|
| Maximum Loading Volume Per Well |
500 μL
|
| Size of DNA Purified |
100 - 15,000 bp
|
| Average DNA Recovery |
> 90%
|
| Average Primer Reoval |
> 90%
|
| Minimum Elution Volume |
50 μL
|
| Time to Complete 96 Purifications |
20 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
|
Kit Specifications - Spin Column
|
|
| Column Binding Capacity |
10 μg
|
| Size of DNA Purified |
100 - 15,000 bp
|
| Average Recovery |
> 90%
|
| Average Primer Removal |
> 90%
|
| Minimum Elution Volume |
30 μL
|
| Time to Complete 10 Purifications |
15 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
| Title | Molecular Confirmation, Identification and Influence of Carbon Source for the Production of Xylanase from Penicillium Citrinum |
| Journal | Journal of Drug Delivery and Therapeutics. 2015. |
| Authors | Naveen M and Siddalingeshwara K G |
| Title | Screening and Identification of Ligninolytic Bacteria for the Treatment of Pulp and Paper Mill Effluent |
| Journal | Water, Air, & Soil Pollution. 2015. |
| Authors | Rajni Hooda, Nishi K. Bhardwaj, Pamela Singh |
| Title | AtROS1 overexpression provides evidence for epigenetic regulation of genes encoding enzymes of flavonoid biosynthesis and antioxidant pathways during salt stress in transgenic tobacco |
| Journal | Journal of Experimental Biotechnology. 2015. |
| Authors | Poonam Bharti, Monika Mahajan, Ajay K. Vishwakarma, Jyoti Bhardwaj and Sudesh Kumar Yadav |
| Title | Recent characterization of cowpea aphid-borne mosaic virus (CABMV) in Bahia State, Brazil, suggests potential regional isolation |
| Journal | African Journal of Biotechnology. 2015. |
| Authors | Jose Romario Fernandes de Melo, Antonia dos Reis Figueira, Charles Neris Moreira, Antonio Carlos de Oliveira. |
| Title | Isolation of Bacteria from Soil sample for ExoPolysaccharide production |
| Journal | International Journal of ChemTech Research. 2014. |
| Authors | A. Gowsalya, V. Ponnusami and K.R. Sugumaran. |
| Title | Isolation and Molecular Identification of Extreme an Halotolerant Staphylococcus Sp. 13cc from Brackish Water of Chilika Lake. |
| Journal | Biosciences Biotechnology Research Asia. 2014. |
| Authors | P.D. Mahapatra and B.B. Mishra. |
| Title | Simultaneous cellulose hydrolysis and bio-electricity generation in a mediatorless Microbial Fuel Cell using a Bacillus flexus strain isolated from wastewater. |
| Journal | Research In Biotechnology. 2014. |
| Authors | Shankar N, Panchapakesan A, Bhandari S, Ravishankar H. |
| Title | TAS2R38 single nucleotide polymorphisms are associated with PROP- but not thermal tasting: a pilot study. |
| Journal | Chemosensory Perception. 2013. |
| Authors | Bering A, Pickering G, Liang P. |
| Title | ”First Generation” Automated DNA Sequencing Technology. |
| Journal | Automated DNA Sequencing Technology. Current Protocols in Molecular Biology. 2011. |
| Authors | Slatko B, Kieleczawa J, Ju J, Gardner A, Hendrickson C, and Ausubel F. |
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