PCR Purification Kit For the rapid purification of amplified DNA products from PCR mixes For the rapid purification of amplified DNA products from PCR mixes Powered by Bioz PCR Purification Kit For the rapid purification of amplified DNA products from PCR mixes Price USD$119.00 SKU 14400 Format Spin Column High Throughput Size 50 Preps 250 Preps Quantity Overview Details Documentation Citations Overview Purify amplified DNA ranging from 100 bp -15,000 bp in size Fast and efficient spin column format Available in a 50 prep size and a 250 prep size Also available in 96 well format This kit enables the rapid purification of amplified DNA products from PCR mixes. It is able to effectively remove PCR by-products including primers, dimers, enzymes, unincorporated nucleotides and mineral oil from the desired PCR product. The purified PCR products are fully compatible with restriction enzyme digestion, ligation into vectors, labeling, sequencing and more. This kit can also be used as an alternative to organic extraction and ethanol precipitation to clean up various enzymatic reactions. The kit is also available in a 96-well format for high-throughput PCR purification. Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation. Details Supporting Data Previous Figure 1. High Yield Purification. The high yield of Norgen's PCR Purification Kit is illustrated by purifying 3.5 µg of a 500 bp PCR-amplified product from a reaction mixture and comparing the recovery with a competitor. The purifications were performed in triplicate. DNA recovery was quantified by running 350 ng (1/10th) of the input and 5 µL (1/10th) of 50 µL DNA elution on a 1X TAE, 1% agarose gel followed by densitometry. The average yield for each kit is shown in Figure 1. Norgen's kit shows a significantly higher recovery than the competitor's kit. Figure 2. Removal of Primer-Dimers. Primer-dimers present in PCR reactions are effectively removed during the purification process with Norgen's PCR Purification Kit. One microgram of a 700 bp PCR fragment, spiked with 250 pmole of primer (Lane 1) was purified using Norgen's kit, with high recovery and no traces of spiked primer present in the elutions (Lanes 2 and 3). Lane 1 contains 400 ng (40%) of the input, and Lanes 2 and 3 contain 20 µL (40%) of the 50 µL elution (duplicate). Lane M is Norgen's MidRanger 1kb DNA Ladder. Eluted DNA was resolved on a 1X TAE, 1% agarose gel. Next Click for expanded view Kit Specifications - Spin Column Column Binding Capacity 10 μg Size of DNA Purified 100 - 15,000 bp Average Recovery > 90% Average Primer Removal > 90% Minimum Elution Volume 30 μL Time to Complete 10 Purifications 15 minutes Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. Component Cat. 14400 (50 preps) Cat. 45700 (250 preps) Cat. 24800 (192 preps) Binding Buffer C 30 mL 5 x 30 mL 3 x 30 mL Wash Solution A 12 mL 2 x 20 mL 2 x 38 mL Elution Buffer B 8 mL 2 x 30 mL 2 x 15 mL Spin Columns 50 250 - Collection Tubes 50 250 - 96-Well Plate - - 2 Adhesive Tape - - 4 96-Well Collection Plate - - 2 Elution Tubes (1.7 mL) 50 250 - 96-Well Elution Plate - - 2 Product Insert 1 1 2 Documentation PROTOCOLS (14400) PCR Purification Kit (Spin Column) - Protocol (50 prep) (45700) PCR Purification Kit (Spin Column) - Protocol (250 prep) (24800) PCR Purification 96-Well Kit (High Throughput) - Protocol (96-well) SAFETY DATA SHEETS (14400, 45700, 24800) PCR Purification Kit - Safety Data Sheet POSTERS The Inhibitors of Polymerase Chain Reaction in a Biological Culture Citations Powered by Bioz See more details on Bioz Related Products DNA Gel Extraction Kit Sequencing Reaction Clean-Up Kit
PCR Purification Kit For the rapid purification of amplified DNA products from PCR mixes For the rapid purification of amplified DNA products from PCR mixes Powered by Bioz PCR Purification Kit For the rapid purification of amplified DNA products from PCR mixes Price USD$119.00 SKU 14400 Format Spin Column High Throughput Size 50 Preps 250 Preps Quantity