For the rapid purification of amplified DNA products from PCR mixes

  • Purify amplified DNA ranging from 100 bp -15,000 bp in size
  • Fast and efficient spin column format
  • Available in a 50 prep size and a 250 prep size
  • Also available in 96 well format

ItemsCat. #Size
PCR Purification Kit 14400 50 preps
PCR Purification 96-Well Kit 24800 192 preps
PCR Purification Kit 45700 250 preps

PCR Purification Kit

This kit enables the rapid purification of amplified DNA products from PCR mixes. It is able to effectively remove PCR by-products including primers, dimers, enzymes, unincorporated nucleotides and mineral oil from the desired PCR product. The purified PCR products are fully compatible with restriction enzyme digestion, ligation into vectors, labeling, sequencing and more. This kit can also be used as an alternative to organic extraction and ethanol precipitation to clean up various enzymatic reactions.

The kit is also available in a 96-well format for high-throughput PCR purification. Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation.

Kit Specifications - Spin Column
Column Binding Capacity
10 μg
Size of DNA Purified
100 - 15,000 bp
Average Recovery
> 90%
Average Primer Removal
> 90%
Minimum Elution Volume
30 μL
Time to Complete 10 Purifications
15 minutes
Kit Specifications - 96-well
Binding Capacity Per Well
15 μg
Maximum Loading Volume Per Well
500 μL
Size of DNA Purified
100 - 15,000 bp
Average DNA Recovery
> 90%
Average Primer Reoval
> 90%
Minimum Elution Volume
50 μL
Time to Complete 96 Purifications
20 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.

Kit Components
Component Cat. 14400 (50 preps) Cat. 45700 (250 preps)
Binding Buffer C 30 mL 5 x 30 mL
Wash Solution A 12 mL 2 x 20 mL
Elution Buffer B 8 mL 2 x 30 mL
Spin Columns 50 250
Collection Tubes 50 250
Elution Tubes (1.7 mL) 50 250
Product Insert 1 1
Component Cat. 24800 (2 x 96-well)
Binding Buffer C 3 x 30 mL
Wash Solution A 2 x 38 mL
Elution Buffer B 2 x 15 mL
96-Well Plate 2
Adhesive Tape 4
96-Well Collection Plate 2
96-Well Elution Plate 2
Product Insert 1

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