PCR Purification Kit

Cat. 14400, 24800, 45700
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PCR Purification Kit

Cat. 14400, 24800, 45700

For the rapid purification of amplified DNA products from PCR mixes

  • Purify amplified DNA ranging from 100 bp -15,000 bp in size
  • Fast and efficient spin column format 
  • Available in a 50 prep size and a 250 prep size
  • Also available in 96 well format
     

For the rapid purification of amplified DNA products from PCR mixes

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PCR Purification Kit
(Cat. 14400)
50 preps
PCR Purification 96-Well Kit
(Cat. 24800)
2 x 96-well plate
PCR Purification Kit
(Cat. 45700)
250 preps

Kit Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Kit Size

  • Purify amplified DNA ranging from 100 bp -15,000 bp in size
  • Fast and efficient spin column format 
  • Available in a 50 prep size and a 250 prep size
  • Also available in 96 well format
     

PCR Purification Kit

This kit enables the rapid purification of amplified DNA products from PCR mixes. It is able to effectively remove PCR by-products including primers, dimers, enzymes, unincorporated nucleotides and mineral oil from the desired PCR product. The purified PCR products are fully compatible with restriction enzyme digestion, ligation into vectors, labeling, sequencing and more. This kit can also be used as an alternative to organic extraction and ethanol precipitation to clean up various enzymatic reactions.

The kit is also available in a 96-well format for high-throughput PCR purification. Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation.

Kit Specifications

You have selected: Cat. 14400
Kit Specifications - Spin Column
Column Binding Capacity
10 μg
Size of DNA Purified
100 - 15,000 bp
Average Recovery
> 90%
Average Primer Removal
> 90%
Minimum Elution Volume
30 μL
Time to Complete 10 Purifications
15 minutes

 
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

You have selected: Cat. 24800
Kit Specifications - 96-well
Binding Capacity Per Well
15 μg
Maximum Loading Volume Per Well
500 μL
Size of DNA Purified
100 - 15,000 bp
Average DNA Recovery
> 90%
Average Primer Reoval
> 90%
Minimum Elution Volume
50 μL
Time to Complete 96 Purifications
20 minutes

 
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

You have selected: Cat. 45700
Kit Specifications - Spin Column
Column Binding Capacity
10 μg
Size of DNA Purified
100 - 15,000 bp
Average Recovery
> 90%
Average Primer Removal
> 90%
Minimum Elution Volume
30 μL
Time to Complete 10 Purifications
15 minutes

 
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Supporting Data

Click for expanded view

Component Cat. 14400 (50 preps) Cat. 45700 (250 preps) Cat. 24800 (192 preps)
Binding Buffer C 30 mL 5 x 30 mL 3 x 30 mL
Wash Solution A 12 mL 2 x 20 mL 2 x 38 mL
Elution Buffer B 8 mL 2 x 30 mL 2 x 15 mL
Spin Columns 50 250 -
Collection Tubes 50 250 -
96-Well Plate - - 2
Adhesive Tape - - 4
96-Well Collection Plate - - 2
Elution Tubes (1.7 mL) 50 250 -
96-Well Elution Plate - - 2
Product Insert 1 1 2

Title Molecular Confirmation, Identification and Influence of Carbon Source for the Production of Xylanase from Penicillium Citrinum
Journal Journal of Drug Delivery and Therapeutics. 2015.
Authors Naveen M and Siddalingeshwara K G
Title Screening and Identification of Ligninolytic Bacteria for the Treatment of Pulp and Paper Mill Effluent
Journal Water, Air, & Soil Pollution. 2015.
Authors Rajni Hooda, Nishi K. Bhardwaj, Pamela Singh
Title AtROS1 overexpression provides evidence for epigenetic regulation of genes encoding enzymes of flavonoid biosynthesis and antioxidant pathways during salt stress in transgenic tobacco
Journal Journal of Experimental Biotechnology. 2015.
Authors Poonam Bharti, Monika Mahajan, Ajay K. Vishwakarma, Jyoti Bhardwaj and Sudesh Kumar Yadav
Title Recent characterization of cowpea aphid-borne mosaic virus (CABMV) in Bahia State, Brazil, suggests potential regional isolation
Journal African Journal of Biotechnology. 2015.
Authors Jose Romario Fernandes de Melo, Antonia dos Reis Figueira, Charles Neris Moreira, Antonio Carlos de Oliveira.
Title Isolation of Bacteria from Soil sample for ExoPolysaccharide production
Journal International Journal of ChemTech Research. 2014.
Authors A. Gowsalya, V. Ponnusami and K.R. Sugumaran.
Title Isolation and Molecular Identification of Extreme an Halotolerant Staphylococcus Sp. 13cc from Brackish Water of Chilika Lake.
Journal Biosciences Biotechnology Research Asia. 2014.
Authors P.D. Mahapatra and B.B. Mishra.
Title Simultaneous cellulose hydrolysis and bio-electricity generation in a mediatorless Microbial Fuel Cell using a Bacillus flexus strain isolated from wastewater.
Journal Research In Biotechnology. 2014.
Authors Shankar N, Panchapakesan A, Bhandari S, Ravishankar H.
Title TAS2R38 single nucleotide polymorphisms are associated with PROP- but not thermal tasting: a pilot study.
Journal Chemosensory Perception. 2013.
Authors Bering A, Pickering G, Liang P.
Title ”First Generation” Automated DNA Sequencing Technology.
Journal Automated DNA Sequencing Technology. Current Protocols in Molecular Biology. 2011.
Authors Slatko B, Kieleczawa J, Ju J, Gardner A, Hendrickson C, and Ausubel F.

PCR Purification Kit

This kit enables the rapid purification of amplified DNA products from PCR mixes. It is able to effectively remove PCR by-products including primers, dimers, enzymes, unincorporated nucleotides and mineral oil from the desired PCR product. The purified PCR products are fully compatible with restriction enzyme digestion, ligation into vectors, labeling, sequencing and more. This kit can also be used as an alternative to organic extraction and ethanol precipitation to clean up various enzymatic reactions.

The kit is also available in a 96-well format for high-throughput PCR purification. Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation.

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 14400
Kit Specifications - Spin Column
Column Binding Capacity
10 μg
Size of DNA Purified
100 - 15,000 bp
Average Recovery
> 90%
Average Primer Removal
> 90%
Minimum Elution Volume
30 μL
Time to Complete 10 Purifications
15 minutes

 
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

You have selected: Cat. 24800
Kit Specifications - 96-well
Binding Capacity Per Well
15 μg
Maximum Loading Volume Per Well
500 μL
Size of DNA Purified
100 - 15,000 bp
Average DNA Recovery
> 90%
Average Primer Reoval
> 90%
Minimum Elution Volume
50 μL
Time to Complete 96 Purifications
20 minutes

 
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

You have selected: Cat. 45700
Kit Specifications - Spin Column
Column Binding Capacity
10 μg
Size of DNA Purified
100 - 15,000 bp
Average Recovery
> 90%
Average Primer Removal
> 90%
Minimum Elution Volume
30 μL
Time to Complete 10 Purifications
15 minutes

 
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Component Cat. 14400 (50 preps) Cat. 45700 (250 preps) Cat. 24800 (192 preps)
Binding Buffer C 30 mL 5 x 30 mL 3 x 30 mL
Wash Solution A 12 mL 2 x 20 mL 2 x 38 mL
Elution Buffer B 8 mL 2 x 30 mL 2 x 15 mL
Spin Columns 50 250 -
Collection Tubes 50 250 -
96-Well Plate - - 2
Adhesive Tape - - 4
96-Well Collection Plate - - 2
Elution Tubes (1.7 mL) 50 250 -
96-Well Elution Plate - - 2
Product Insert 1 1 2

Title Molecular Confirmation, Identification and Influence of Carbon Source for the Production of Xylanase from Penicillium Citrinum
Journal Journal of Drug Delivery and Therapeutics. 2015.
Authors Naveen M and Siddalingeshwara K G
Title Screening and Identification of Ligninolytic Bacteria for the Treatment of Pulp and Paper Mill Effluent
Journal Water, Air, & Soil Pollution. 2015.
Authors Rajni Hooda, Nishi K. Bhardwaj, Pamela Singh
Title AtROS1 overexpression provides evidence for epigenetic regulation of genes encoding enzymes of flavonoid biosynthesis and antioxidant pathways during salt stress in transgenic tobacco
Journal Journal of Experimental Biotechnology. 2015.
Authors Poonam Bharti, Monika Mahajan, Ajay K. Vishwakarma, Jyoti Bhardwaj and Sudesh Kumar Yadav
Title Recent characterization of cowpea aphid-borne mosaic virus (CABMV) in Bahia State, Brazil, suggests potential regional isolation
Journal African Journal of Biotechnology. 2015.
Authors Jose Romario Fernandes de Melo, Antonia dos Reis Figueira, Charles Neris Moreira, Antonio Carlos de Oliveira.
Title Isolation of Bacteria from Soil sample for ExoPolysaccharide production
Journal International Journal of ChemTech Research. 2014.
Authors A. Gowsalya, V. Ponnusami and K.R. Sugumaran.
Title Isolation and Molecular Identification of Extreme an Halotolerant Staphylococcus Sp. 13cc from Brackish Water of Chilika Lake.
Journal Biosciences Biotechnology Research Asia. 2014.
Authors P.D. Mahapatra and B.B. Mishra.
Title Simultaneous cellulose hydrolysis and bio-electricity generation in a mediatorless Microbial Fuel Cell using a Bacillus flexus strain isolated from wastewater.
Journal Research In Biotechnology. 2014.
Authors Shankar N, Panchapakesan A, Bhandari S, Ravishankar H.
Title TAS2R38 single nucleotide polymorphisms are associated with PROP- but not thermal tasting: a pilot study.
Journal Chemosensory Perception. 2013.
Authors Bering A, Pickering G, Liang P.
Title ”First Generation” Automated DNA Sequencing Technology.
Journal Automated DNA Sequencing Technology. Current Protocols in Molecular Biology. 2011.
Authors Slatko B, Kieleczawa J, Ju J, Gardner A, Hendrickson C, and Ausubel F.

 


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