PCR Purification Kit

Cat. 14400, 24800, 45700

PCR Purification Kit

Cat.

For the rapid purification of amplified DNA products from PCR mixes

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PCR Purification Kit
(Cat. 14400) 50 preps

PCR Purification 96-Well Kit
(Cat. 24800) 2 x 96-well plate

PCR Purification Kit
(Cat. 45700) 250 preps

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Format: Spin Column
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Size:50 preps

Overview

PCR Purification Kit

Purify amplified DNA ranging from 100 bp -15,000 bp in size
Fast and efficient spin column format
Available in a 50 prep size and a 250 prep size
Also available in 96 well format

This kit enables the rapid purification of amplified DNA products from PCR mixes. It is able to effectively remove PCR by-products including primers, dimers, enzymes, unincorporated nucleotides and mineral oil from the desired PCR product. The purified PCR products are fully compatible with restriction enzyme digestion, ligation into vectors, labeling, sequencing and more. This kit can also be used as an alternative to organic extraction and ethanol precipitation to clean up various enzymatic reactions.

The kit is also available in a 96-well format for high-throughput PCR purification. Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation.

Details

Supporting Data

Figure 1. High Yield Purification. The high yield of Norgen's PCR Purification Kit is illustrated by purifying 3.5 µg of a 500 bp PCR-amplified product from a reaction mixture and comparing the recovery with a competitor. The purifications were performed in triplicate. DNA recovery was quantified by running 350 ng (1/10th) of the input and 5 µL (1/10th) of 50 µL DNA elution on a 1X TAE, 1% agarose gel followed by densitometry. The average yield for each kit is shown in Figure 1. Norgen's kit shows a significantly higher recovery than the competitor's kit.

Figure 2. Removal of Primer-Dimers. Primer-dimers present in PCR reactions are effectively removed during the purification process with Norgen's PCR Purification Kit. One microgram of a 700 bp PCR fragment, spiked with 250 pmole of primer (Lane 1) was purified using Norgen's kit, with high recovery and no traces of spiked primer present in the elutions (Lanes 2 and 3). Lane 1 contains 400 ng (40%) of the input, and Lanes 2 and 3 contain 20 µL (40%) of the 50 µL elution (duplicate). Lane M is Norgen's MidRanger 1kb DNA Ladder. Eluted DNA was resolved on a 1X TAE, 1% agarose gel.

Kit Specifications

Cat.

Kit Specifications - Spin Column
Column Binding Capacity	10 μg
Size of DNA Purified	100 - 15,000 bp
Average Recovery	> 90%
Average Primer Removal	> 90%
Minimum Elution Volume	30 μL
Time to Complete 10 Purifications	15 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Kit Specifications - 96-well
Binding Capacity Per Well	15 μg
Maximum Loading Volume Per Well	500 μL
Size of DNA Purified	100 - 15,000 bp
Average DNA Recovery	> 90%
Average Primer Reoval	> 90%
Minimum Elution Volume	50 μL
Time to Complete 96 Purifications	20 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Kit Specifications - Spin Column
Column Binding Capacity	10 μg
Size of DNA Purified	100 - 15,000 bp
Average Recovery	> 90%
Average Primer Removal	> 90%
Minimum Elution Volume	30 μL
Time to Complete 10 Purifications	15 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

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PCR Purification Kit (Cat. 14400, 24800, 45700)

PCR Purification Kit

PCR Purification Kit

Format: Spin Column
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Size:50 preps