Ideal for assessment of a range of PCR product sizes

  • Ready-to-use
  • Quantitative
  • Highly Stable
  • Precise
  • Eleven discrete fragments ranging from 50 bp to 1000 bp
  • Higher intensity reference band at 500 bp

ItemsCat. #Size
PCR Ranger 100 bp DNA Ladder 11300 100 loads

PCR Ranger 100 bp DNA Ladder

The Norgen PCR Ranger 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our PCR Ranger DNA Ladder contains eleven discrete fragments ranging from 50 bp to 1000 bp with a higher intensity reference band at 500 bp. This Ladder is ideal for assessment of a range of PCR product sizes.

Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

PCR Ranger 100 bp DNA Ladder (Cat# 11300) - 100 loads

Ladder Properties:
• Eleven discrete bands, ranging from 50 bp to 1,000 bp
• Higher intensity band at 500 bp for easy reference

Fragment
Size (bp)
Mass (ng)
1
1000
91
2
900
66
3
800
61
4
700
51
5
600
43
6
500
70
7
400
28
8
300
23
9
200
30
10
100
22
11
50
15

Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.

Title Rapid Allele-Specific PCR method for CDA 79A>C (K27Q) genotyping: A useful pharmacogenetic tool and world-wide polymorphism distribution.
Journal Clinica Chimica Acta. 2011.
Authors Carpia F, Xub J, Vincenzettic S, Vitac A, Caib W, Napolionia V.
Title The influence of initial feeding on muscle development and growth in pacu Piaractus mesopotamicus larvae.
Journal Aquaculture. 2011.
Authors Leitão N, Pai-Silva M, Losi Alves de Almeida F, Portella M.