PCR Ranger 100 bp DNA Ladder

Cat. 11300

PCR Ranger 100 bp DNA Ladder

Cat.

Ideal for assessment of a range of PCR product sizes

For research use only and NOT intended for in vitro diagnostics.

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PCR Ranger 100 bp DNA Ladder
(Cat. 11300) 100 loads

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Size:100 loads

Overview

PCR Ranger 100 bp DNA Ladder

Ready-to-use
Quantitative
Highly Stable
Precise
Eleven discrete fragments ranging from 50 bp to 1000 bp
Higher intensity reference band at 500 bp

The Norgen PCR Ranger 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our PCR Ranger DNA Ladder contains eleven discrete fragments ranging from 50 bp to 1000 bp with a higher intensity reference band at 500 bp. This Ladder is ideal for assessment of a range of PCR product sizes.

Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

Details

Supporting Data

Figure 1. PCRRanger 100 bp DNALadder.

Kit Specifications

Cat.

PCR Ranger 100 bp DNA Ladder (Cat# 11300) - 100 loads

Ladder Properties:
• Eleven discrete bands, ranging from 50 bp to 1,000 bp
• Higher intensity band at 500 bp for easy reference

Fragment	Size (bp)	Mass (ng)
1	1000	91
2	900	66
3	800	61
4	700	51
5	600	43
6	500	70
7	400	28
8	300	23
9	200	30
10	100	22
11	50	15

Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.

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PCR Ranger 100 bp DNA Ladder (Cat. 11300)

PCR Ranger 100 bp DNA Ladder

PCR Ranger 100 bp DNA Ladder

Size:100 loads

Overview

PCR Ranger 100 bp DNA Ladder

Details

Supporting Data

Kit Specifications

Documentation

Citations