PCR Sizer 100 bp DNA Ladder (Cat. 11400)
PCR Sizer 100 bp DNA Ladder
Cat. 11400
![Norgen Biotek DNA Ladders](https://norgenbiotek.com/sites/default/files/styles/original/public/ladders_1.jpg?itok=UFMvbcKX" "PCR Sizer 100 bp DNA Ladder")
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PCR Sizer 100 bp DNA Ladder
Cat. 11400
Ideal for PCR product size confirmation
- Ready-to-use
- Quantitative
- Highly Stable
- Precise
- Ten discrete fragments from 100 bp to 1000 bp
- Higher intensity reference band at 500 bp
For research use only and NOT intended for in vitro diagnostics.
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PCR Sizer 100 bp DNA Ladder
(Cat. 11400) 100 loads
(Cat. 11400) 100 loads
Product Size
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Kit Specifications
You have selected: Cat. 11400
PCR Sizer 100 bp DNA Ladder (Cat# 11400) - 100 loads
Ladder Properties:
• Ten discrete bands, ranging from 100 bp to 1,000 bp
• Higher intensity band at 500 bp for easy reference
• Ten discrete bands, ranging from 100 bp to 1,000 bp
• Higher intensity band at 500 bp for easy reference
|
Fragment
|
Size (bp)
|
Mass (ng)
|
|
1
|
1000
|
78
|
|
2
|
900
|
70
|
|
3
|
800
|
62
|
|
4
|
700
|
55
|
|
5
|
600
|
47
|
|
6
|
500
|
78
|
|
7
|
400
|
31
|
|
8
|
300
|
24
|
|
9
|
200
|
31
|
|
10
|
100
|
24
|
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
Supporting Data
Click for expanded view
Protocols:
Application Notes:
Safety Data Sheets:
| Title | Virulence Genes of Pathogenic Escherichia coli in Wild Red Foxes (Vulpes vulpes) |
| Journal | Animals . 2017. |
| Authors | Fabrizio Bertelloni, Giulia Cagnoli, Fabrizio Biagini, Alessandro Poli, Carlo Bibbiani and Valentina Virginia Ebani |
| Title | Distribution Profile of Extended Spectrum Beta Lactamase (ESBL) Producing Escherichia coli Isolates from Asa River (Nigeria) |
| Journal | Journal of Applied Sciences and Environmental Management. 2017. |
| Authors | Matthew Kolawole Olatunji, Albert Olayemi, Fausat Jimoh |
| Title | Standardization of a molecular method for epidemiologic identification of Leishmania strains |
| Journal | Genet. Mol. Res. 2016. |
| Authors | D.A. Oliveira, E.V. Menezes, V.A. Royo, A.R.E.O. Xavier, R.F. Rocha, A.F.M. Júnior, E.S. Dias, A.C.V.M.R. Lima, E.M. Michalsky |
| Title | Occurrence and molecular analysis of quarantine virus in lily cultivation areas in Brazil |
| Journal | Pesq. agropec. bras., Brasília. 2016. |
| Authors | Rivas, E. B., de Almeida Bôdi, E. C., Harakava, R., Gregori, F., & Gonçalves, M. C |
| Title | Food control and a citizen science approach for improving teaching of Genetics in universities |
| Journal | IUBMB Journals. 2016. |
| Authors | Borrell YJ, Muñoz-Colmenero AM, Dopico E, Miralles L, Garcia-Vazquez E |
| Title | Establishment of a bank of positive controls for diagnosis of quarantine viruses and viroids in Brazil through PCR and RT-PCR |
| Journal | Crop Protection. 2016. |
| Authors | Priscilla De Sousa Geraldino Duarte, Antonia Dos Reis Figueira, Suellen Barbara Ferreira Galvino-Costa, Anderson De Jesus Sotero, Daniele Costa Pompeu, João Ronaldo Clemente Fernandes, André Luiz De Andrade Carvalho |
| Title | Is Malassezia nana the main species in horses’ ear canal microbiome? |
| Journal | Brazilian Journal of Microbiology. 2016. |
| Authors | Aldrovandi, A. L., Osugui, L., & Coutinho, S. D. A |
| Title | Evidence of helminth infection in guanche mummies: Integrating paleoparasitological and paleogenetic investigations |
| Journal | Journal of Parasitology. 2015. |
| Authors | Iniguez AM, Gijà N-Botella H, Jaeger L, Del Arco-Aguilar MA, Del Arco-Aguilar MA, Araujo A, Rodrà Guez-Maffiotte C, Martà N-Oval M. |
| Title | Analysis of Immunological, Viral, Genetic, and Environmental Factors That Might Be Associated with Decreased Susceptibility to HIV Infection in Serodiscordant Couples in Florianópolis, Southern Brazil |
| Journal | AIDS Research and Human Retroviruses. 2015. |
| Authors | Santos ÍM, da Rosa EA, Gräf T, Ferreira LG, Petry A, Cavalheiro F, Reiche EM, Zanetti CR, Pinto AR |
| Title | Escherichia coli from Crohn’s disease patient displays virulence features of enteroinvasive (EIEC), enterohemorragic (EHEC), and enteroaggregative (EAEC) pathotypes. |
| Journal | Gut Pathogens. 2015. |
| Authors | Ana Carolina da Silva Santos, Fernando Gomes Romeiro, Ligia Yukie Sassaki and Josias Rodrigues |
| Title | Pathogenesis of acute and chronic diseases caused by cyprinid herpesvirus-3. |
| Journal | Journal of Fish Diseases. 2014. |
| Authors | S Miwa, I Kiryu, K Yuasa, T Ito and T Kaneko. |
| Title | Geography has more influence than language on maternal genetic structure of various northeastern Thai ethnicities. |
| Journal | Journal of Human Genetics. 2014. |
| Authors | Wibhu Kutanan1, Silvia Ghirotto, Giorgio Bertorelle, Suparat Srithawong, Kanokpohn Srithongdaeng,Nattapon Pontham and Daoroong Kangwanpong |
| Title | Genetic diversity in the germplasm of tropical maize landraces determined using molecular markers. |
| Journal | Genetic and Molecular Research. 2013. |
| Authors | Molin D, Coelho CJ, Mˆximo DS, Ferreira FS, Gardingo JR, Matiello RR. |
| Title | Characterization of denitrifying granular sludge with and without the addition of external carbon source. |
| Journal | Bioresource Technology. 2012. |
| Authors | Lew B, Stief P, Beliavski M, Ashkenazi A, Svitlica O, Khan A, Tarre S, de Beer D, Green M. |
| Title | Sequencing and expression analysis of hepcidin mRNA in donkey (Equus asinus) liver. |
| Journal | Pesq. Vet. Bras. 2012. |
| Authors | Oliveira-Filho J, Marques J, Cunha P, Medeiros G, Riet-Correa F, Machado V, Borges A. |
| Title | Unraveling a mechanism of honey antibacterial action: Polyphenol/H2O2-induced oxidative effect on bacterial cell growth and on DNA degradation. |
| Journal | Food Chemistry. 2012. |
| Authors | Brudzynski K, Abubaker K, Miotto D. |
| Title | Re-examining the role of hydrogen peroxide in bacteriostatic and bactericidal activities of honey. |
| Journal | Frontiers in Microbiology. 2011. |
| Authors | Brudzynski K, Abubaker K, Laurent M, Castle A. |
| Title | The influence of initial feeding on muscle development and growth in pacu Piaractus mesopotamicus larvae. |
| Journal | Aquaculture. 2011. |
| Authors | Natalia de Jesus Leitão, Maeli Dal Pai-Silva, Fernanda Losi Alves de Almeida, Maria Célia Portella. |
| Title | The beta4-beta8 groove is an ATP-interactive site in the alpha crystallin core domain of the small heat shock protein, human alphaB crystallin. |
| Journal | Journal of Molecular Biology. 2006. |
| Authors | Ghosh JG, Houck SA, Doneanu CE, Clark JI. |
PCR Sizer 100 bp DNA Ladder
The Norgen PCRSizer 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our PCRSizer contains ten discrete fragments ranging from 100 bp to 1000 bp in 100 bp increments with a higher intensity reference band at 500 bp. This Ladder is ideal for PCR product size confirmation.
Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
Supporting Data
Click for expanded view
Kit Specifications
You have selected: Cat. 11400
PCR Sizer 100 bp DNA Ladder (Cat# 11400) - 100 loads
Ladder Properties:
• Ten discrete bands, ranging from 100 bp to 1,000 bp
• Higher intensity band at 500 bp for easy reference
• Ten discrete bands, ranging from 100 bp to 1,000 bp
• Higher intensity band at 500 bp for easy reference
|
Fragment
|
Size (bp)
|
Mass (ng)
|
|
1
|
1000
|
78
|
|
2
|
900
|
70
|
|
3
|
800
|
62
|
|
4
|
700
|
55
|
|
5
|
600
|
47
|
|
6
|
500
|
78
|
|
7
|
400
|
31
|
|
8
|
300
|
24
|
|
9
|
200
|
31
|
|
10
|
100
|
24
|
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
Protocols:
Application Notes:
Safety Data Sheets:
| Title | Virulence Genes of Pathogenic Escherichia coli in Wild Red Foxes (Vulpes vulpes) |
| Journal | Animals . 2017. |
| Authors | Fabrizio Bertelloni, Giulia Cagnoli, Fabrizio Biagini, Alessandro Poli, Carlo Bibbiani and Valentina Virginia Ebani |
| Title | Distribution Profile of Extended Spectrum Beta Lactamase (ESBL) Producing Escherichia coli Isolates from Asa River (Nigeria) |
| Journal | Journal of Applied Sciences and Environmental Management. 2017. |
| Authors | Matthew Kolawole Olatunji, Albert Olayemi, Fausat Jimoh |
| Title | Standardization of a molecular method for epidemiologic identification of Leishmania strains |
| Journal | Genet. Mol. Res. 2016. |
| Authors | D.A. Oliveira, E.V. Menezes, V.A. Royo, A.R.E.O. Xavier, R.F. Rocha, A.F.M. Júnior, E.S. Dias, A.C.V.M.R. Lima, E.M. Michalsky |
| Title | Occurrence and molecular analysis of quarantine virus in lily cultivation areas in Brazil |
| Journal | Pesq. agropec. bras., Brasília. 2016. |
| Authors | Rivas, E. B., de Almeida Bôdi, E. C., Harakava, R., Gregori, F., & Gonçalves, M. C |
| Title | Food control and a citizen science approach for improving teaching of Genetics in universities |
| Journal | IUBMB Journals. 2016. |
| Authors | Borrell YJ, Muñoz-Colmenero AM, Dopico E, Miralles L, Garcia-Vazquez E |
| Title | Establishment of a bank of positive controls for diagnosis of quarantine viruses and viroids in Brazil through PCR and RT-PCR |
| Journal | Crop Protection. 2016. |
| Authors | Priscilla De Sousa Geraldino Duarte, Antonia Dos Reis Figueira, Suellen Barbara Ferreira Galvino-Costa, Anderson De Jesus Sotero, Daniele Costa Pompeu, João Ronaldo Clemente Fernandes, André Luiz De Andrade Carvalho |
| Title | Is Malassezia nana the main species in horses’ ear canal microbiome? |
| Journal | Brazilian Journal of Microbiology. 2016. |
| Authors | Aldrovandi, A. L., Osugui, L., & Coutinho, S. D. A |
| Title | Evidence of helminth infection in guanche mummies: Integrating paleoparasitological and paleogenetic investigations |
| Journal | Journal of Parasitology. 2015. |
| Authors | Iniguez AM, Gijà N-Botella H, Jaeger L, Del Arco-Aguilar MA, Del Arco-Aguilar MA, Araujo A, Rodrà Guez-Maffiotte C, Martà N-Oval M. |
| Title | Analysis of Immunological, Viral, Genetic, and Environmental Factors That Might Be Associated with Decreased Susceptibility to HIV Infection in Serodiscordant Couples in Florianópolis, Southern Brazil |
| Journal | AIDS Research and Human Retroviruses. 2015. |
| Authors | Santos ÍM, da Rosa EA, Gräf T, Ferreira LG, Petry A, Cavalheiro F, Reiche EM, Zanetti CR, Pinto AR |
| Title | Escherichia coli from Crohn’s disease patient displays virulence features of enteroinvasive (EIEC), enterohemorragic (EHEC), and enteroaggregative (EAEC) pathotypes. |
| Journal | Gut Pathogens. 2015. |
| Authors | Ana Carolina da Silva Santos, Fernando Gomes Romeiro, Ligia Yukie Sassaki and Josias Rodrigues |
| Title | Pathogenesis of acute and chronic diseases caused by cyprinid herpesvirus-3. |
| Journal | Journal of Fish Diseases. 2014. |
| Authors | S Miwa, I Kiryu, K Yuasa, T Ito and T Kaneko. |
| Title | Geography has more influence than language on maternal genetic structure of various northeastern Thai ethnicities. |
| Journal | Journal of Human Genetics. 2014. |
| Authors | Wibhu Kutanan1, Silvia Ghirotto, Giorgio Bertorelle, Suparat Srithawong, Kanokpohn Srithongdaeng,Nattapon Pontham and Daoroong Kangwanpong |
| Title | Genetic diversity in the germplasm of tropical maize landraces determined using molecular markers. |
| Journal | Genetic and Molecular Research. 2013. |
| Authors | Molin D, Coelho CJ, Mˆximo DS, Ferreira FS, Gardingo JR, Matiello RR. |
| Title | Characterization of denitrifying granular sludge with and without the addition of external carbon source. |
| Journal | Bioresource Technology. 2012. |
| Authors | Lew B, Stief P, Beliavski M, Ashkenazi A, Svitlica O, Khan A, Tarre S, de Beer D, Green M. |
| Title | Sequencing and expression analysis of hepcidin mRNA in donkey (Equus asinus) liver. |
| Journal | Pesq. Vet. Bras. 2012. |
| Authors | Oliveira-Filho J, Marques J, Cunha P, Medeiros G, Riet-Correa F, Machado V, Borges A. |
| Title | Unraveling a mechanism of honey antibacterial action: Polyphenol/H2O2-induced oxidative effect on bacterial cell growth and on DNA degradation. |
| Journal | Food Chemistry. 2012. |
| Authors | Brudzynski K, Abubaker K, Miotto D. |
| Title | Re-examining the role of hydrogen peroxide in bacteriostatic and bactericidal activities of honey. |
| Journal | Frontiers in Microbiology. 2011. |
| Authors | Brudzynski K, Abubaker K, Laurent M, Castle A. |
| Title | The influence of initial feeding on muscle development and growth in pacu Piaractus mesopotamicus larvae. |
| Journal | Aquaculture. 2011. |
| Authors | Natalia de Jesus Leitão, Maeli Dal Pai-Silva, Fernanda Losi Alves de Almeida, Maria Célia Portella. |
| Title | The beta4-beta8 groove is an ATP-interactive site in the alpha crystallin core domain of the small heat shock protein, human alphaB crystallin. |
| Journal | Journal of Molecular Biology. 2006. |
| Authors | Ghosh JG, Houck SA, Doneanu CE, Clark JI. |