• Ready-to-use
  • Quantitative
  • Highly Stable
  • Precise
  • Ten discrete fragments from 100 bp to 1000 bp
  • Higher intensity reference band at 500 bp

ItemsCat. #Size
PCR Sizer 100 bp DNA Ladder 11400 100 loads

PCR Sizer 100 bp DNA Ladder

The Norgen PCRSizer 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our PCRSizer contains ten discrete fragments ranging from 100 bp to 1000 bp in 100 bp increments with a higher intensity reference band at 500 bp. This Ladder is ideal for PCR product size confirmation.

Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

PCR Sizer 100 bp DNA Ladder (Cat# 11400) - 100 loads

Ladder Properties:
• Ten discrete bands, ranging from 100 bp to 1,000 bp
• Higher intensity band at 500 bp for easy reference

 

Fragment
Size (bp)
Mass (ng)
1
 1000
78
2
 900
70
3
 800
62
4
 700
55
5
 600
47
6
 500
78
7
 400
31
8
 300
24
9
 200
31
10
 100
24
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
 
Storage: 
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.

Title Distribution Profile of Extended Spectrum Beta Lactamase (ESBL) Producing Escherichia coli Isolates from Asa River (Nigeria)
Journal Journal of Applied Sciences and Environmental Management. 2017.
Authors Matthew Kolawole Olatunji, Albert Olayemi, Fausat Jimoh
Title Standardization of a molecular method for epidemiologic identification of Leishmania strains
Journal Genet. Mol. Res. 2016.
Authors D.A. Oliveira, E.V. Menezes, V.A. Royo, A.R.E.O. Xavier, R.F. Rocha, A.F.M. Júnior, E.S. Dias, A.C.V.M.R. Lima, E.M. Michalsky
Title Occurrence and molecular analysis of quarantine virus in lily cultivation areas in Brazil
Journal Pesq. agropec. bras., Brasília. 2016.
Authors Rivas, E. B., de Almeida Bôdi, E. C., Harakava, R., Gregori, F., & Gonçalves, M. C
Title Food control and a citizen science approach for improving teaching of Genetics in universities
Journal IUBMB Journals. 2016.
Authors Borrell YJ, Muñoz-Colmenero AM, Dopico E, Miralles L, Garcia-Vazquez E
Title Establishment of a bank of positive controls for diagnosis of quarantine viruses and viroids in Brazil through PCR and RT-PCR
Journal Crop Protection. 2016.
Authors Priscilla De Sousa Geraldino Duarte, Antonia Dos Reis Figueira, Suellen Barbara Ferreira Galvino-Costa, Anderson De Jesus Sotero, Daniele Costa Pompeu, João Ronaldo Clemente Fernandes, André Luiz De Andrade Carvalho
Title Is Malassezia nana the main species in horses’ ear canal microbiome?
Journal Brazilian Journal of Microbiology. 2016.
Authors Aldrovandi, A. L., Osugui, L., & Coutinho, S. D. A
Title Evidence of helminth infection in guanche mummies: Integrating paleoparasitological and paleogenetic investigations
Journal Journal of Parasitology. 2015.
Authors Iniguez AM, Gijà N-Botella H, Jaeger L, Del Arco-Aguilar MA, Del Arco-Aguilar MA, Araujo A, Rodrà Guez-Maffiotte C, Martà N-Oval M.
Title Analysis of Immunological, Viral, Genetic, and Environmental Factors That Might Be Associated with Decreased Susceptibility to HIV Infection in Serodiscordant Couples in Florianópolis, Southern Brazil
Journal AIDS Research and Human Retroviruses. 2015.
Authors Santos ÍM, da Rosa EA, Gräf T, Ferreira LG, Petry A, Cavalheiro F, Reiche EM, Zanetti CR, Pinto AR
Title Escherichia coli from Crohn’s disease patient displays virulence features of enteroinvasive (EIEC), enterohemorragic (EHEC), and enteroaggregative (EAEC) pathotypes.
Journal Gut Pathogens. 2015.
Authors Ana Carolina da Silva Santos, Fernando Gomes Romeiro, Ligia Yukie Sassaki and Josias Rodrigues
Title Pathogenesis of acute and chronic diseases caused by cyprinid herpesvirus-3.
Journal Journal of Fish Diseases. 2014.
Authors S Miwa, I Kiryu, K Yuasa, T Ito and T Kaneko.
Title Geography has more influence than language on maternal genetic structure of various northeastern Thai ethnicities.
Journal Journal of Human Genetics. 2014.
Authors Wibhu Kutanan1, Silvia Ghirotto, Giorgio Bertorelle, Suparat Srithawong, Kanokpohn Srithongdaeng,Nattapon Pontham and Daoroong Kangwanpong
Title Genetic diversity in the germplasm of tropical maize landraces determined using molecular markers.
Journal Genetic and Molecular Research. 2013.
Authors Molin D, Coelho CJ, Mˆximo DS, Ferreira FS, Gardingo JR, Matiello RR.
Title Characterization of denitrifying granular sludge with and without the addition of external carbon source.
Journal Bioresource Technology. 2012.
Authors Lew B, Stief P, Beliavski M, Ashkenazi A, Svitlica O, Khan A, Tarre S, de Beer D, Green M.
Title Sequencing and expression analysis of hepcidin mRNA in donkey (Equus asinus) liver.
Journal Pesq. Vet. Bras. 2012.
Authors Oliveira-Filho J, Marques J, Cunha P, Medeiros G, Riet-Correa F, Machado V, Borges A.
Title Re-examining the role of hydrogen peroxide in bacteriostatic and bactericidal activities of honey.
Journal Frontiers in Microbiology. 2011.
Authors Brudzynski K, Abubaker K, Laurent M, Castle A.
Title The influence of initial feeding on muscle development and growth in pacu Piaractus mesopotamicus larvae.
Journal Aquaculture. 2011.
Authors Natalia de Jesus Leitão, Maeli Dal Pai-Silva, Fernanda Losi Alves de Almeida, Maria Célia Portella.
Title The beta4-beta8 groove is an ATP-interactive site in the alpha crystallin core domain of the small heat shock protein, human alphaB crystallin.
Journal Journal of Molecular Biology. 2006.
Authors Ghosh JG, Houck SA, Doneanu CE, Clark JI.