Plant microRNA Purification Kit (Cat. 54700)

For the rapid purification of microRNA from plants

  • Fast and easy processing using a rapid spin-column format
  • No phenol or chloroform extractions
  • Isolate all small RNA molecules (<200 nt)
  • Minimal contamination from large RNA molecules and genomic DNA
  • High quality small RNA can be used in various downstream applications

ItemsCat. #Size
Plant microRNA Purification Kit 54700 25 preps

Plant microRNA Purification Kit

Norgen’s Plant microRNA Purification Kit provides a rapid method for the isolation and purification of small RNA molecules (< 200 nt) from cultured plant cells or plant tissues. These small RNAs include regulatory RNA molecules such as microRNA (miRNA) and short interfering RNA (siRNA), as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. miRNAs and siRNAs are typically 20-25 nucleotides long, and regulate gene expression by binding to mRNA molecules and affecting their stability or translation. The small RNA molecules isolated using Norgen’s Plant microRNA Purification Kit can be used in various downstream applications relating to gene regulation and functional analysis, including RT-PCR, miRNA sequencing, northern blotting and microarray analysis.

Kit Specifications
Maximum Column Binding Capacity
50 μg
Maximum Column Loading Volume
650 μL
Minimum Elution Volume
20 μL
Size of RNA Purified
< 200 nt
Amount of Starting Material:
Plant Tissues
100 mg
5 x 106 cells
Time to Complete 10 Purifications
25 minutes

 

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.