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For the rapid purification of microRNA from plants
Plant microRNA Purification Kit
Norgen’s Plant microRNA Purification Kit provides a rapid method for the isolation and purification of small RNA molecules (< 200 nt) from cultured plant cells or plant tissues. These small RNAs include regulatory RNA molecules such as microRNA (miRNA) and short interfering RNA (siRNA), as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. miRNAs and siRNAs are typically 20-25 nucleotides long, and regulate gene expression by binding to mRNA molecules and affecting their stability or translation. The small RNA molecules isolated using Norgen’s Plant microRNA Purification Kit can be used in various downstream applications relating to gene regulation and functional analysis, including RT-PCR, miRNA sequencing, northern blotting and microarray analysis.
Kit Specifications
|
|
Maximum Column Binding Capacity
|
50 μg
|
Maximum Column Loading Volume
|
650 μL
|
Minimum Elution Volume |
20 μL
|
Size of RNA Purified |
< 200 nt
|
Amount of Starting Material: Plant Tissues |
100 mg
5 x 106 cells |
Time to Complete 10 Purifications |
25 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
Plant microRNA Purification Kit
Norgen’s Plant microRNA Purification Kit provides a rapid method for the isolation and purification of small RNA molecules (< 200 nt) from cultured plant cells or plant tissues. These small RNAs include regulatory RNA molecules such as microRNA (miRNA) and short interfering RNA (siRNA), as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. miRNAs and siRNAs are typically 20-25 nucleotides long, and regulate gene expression by binding to mRNA molecules and affecting their stability or translation. The small RNA molecules isolated using Norgen’s Plant microRNA Purification Kit can be used in various downstream applications relating to gene regulation and functional analysis, including RT-PCR, miRNA sequencing, northern blotting and microarray analysis.
Kit Specifications
|
|
Maximum Column Binding Capacity
|
50 μg
|
Maximum Column Loading Volume
|
650 μL
|
Minimum Elution Volume |
20 μL
|
Size of RNA Purified |
< 200 nt
|
Amount of Starting Material: Plant Tissues |
100 mg
5 x 106 cells |
Time to Complete 10 Purifications |
25 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
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