Plant microRNA Purification Kit (Cat. 54700)

For the rapid purification of microRNA from plants

Fast and easy processing using a rapid spin-column format
No phenol or chloroform extractions
Isolate all small RNA molecules (<200 nt)
Minimal contamination from large RNA molecules and genomic DNA
High quality small RNA can be used in various downstream applications

Items	Cat. #	Size
Plant microRNA Purification Kit	54700	25 preps

Plant microRNA Purification Kit

Norgen’s Plant microRNA Purification Kit provides a rapid method for the isolation and purification of small RNA molecules (< 200 nt) from cultured plant cells or plant tissues. These small RNAs include regulatory RNA molecules such as microRNA (miRNA) and short interfering RNA (siRNA), as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. miRNAs and siRNAs are typically 20-25 nucleotides long, and regulate gene expression by binding to mRNA molecules and affecting their stability or translation. The small RNA molecules isolated using Norgen’s Plant microRNA Purification Kit can be used in various downstream applications relating to gene regulation and functional analysis, including RT-PCR, miRNA sequencing, northern blotting and microarray analysis.

Kit Specifications
Maximum Column Binding Capacity	50 μg
Maximum Column Loading Volume	650 μL
Minimum Elution Volume	20 μL
Size of RNA Purified	< 200 nt
Amount of Starting Material: Plant Tissues	100 mg 5 x 10⁶ cells
Time to Complete 10 Purifications	25 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.

Kit Components

Component	Cat. 54700 (25 preps)
Lysis Buffer C	30 mL
Wash Solution A	18 mL
Elution Solution A	6 mL
Filter Columns	25
Large RNA Removal Columns	25
microRNA Enrichment Columns	25
Collection Tubes	75
Elution Tubes (1.7 mL)	50
Product Insert	1