For simultaneous isolation of total RNA and DNA from the same plant sample

  • Robust Lysis Solution processes even the most challenging plant species such as pine needle and grape
  • No phenol extractions
  • DNA and all sizes of RNA are recovered, including microRNA
  • High quality DNA and RNA are purified simultaneously using the same spin column
  • No need to split the lysate​

ItemsCat. #Size
Plant RNA/DNA Purification Kit 24400 50 preps

Plant RNA/DNA Purification Kit

This kit provides for rapid spin column isolation and purification of total RNA and genomic DNA simultaneously from a single plant sample without splitting the lyate. Norgen's plant lysis solution is highly robust and effective over a wide range of plants including challenging samples. The total RNA and genomic DNA are both column purified in under 30 minutes. Since RNA and DNA are isolated without splitting the lysate, variability and inconsistent results are reduced. All sizes of RNA including microRNA are recovered without the need for phenol. Optional on-column DNase and RNase treatments provide flexibility to isolate DNA-free RNA or RNA-free DNA respectively. Isolated nucleic acids are of a high quality and yield, and are ready for downstream use including PCR, qPCR, RT-PCR, qRT-PCR, sequencing and more.

Background

It is often necessary to isolate total RNA and genomic DNA from a single plant sample, such as for studies of gene expression, mutant or transgenic plant characterization, and host plant-pathogen characterization. This is of great benefit when isolating RNA and DNA from precious, difficult to obtain or very small samples. Furthermore, gene expression analysis will be more reliable since the RNA and DNA are derived from the same sample, therefore eliminating inconsistent results.

Kit Specifications
Column Binding Capacity
50 μg for RNA
15 μg for genomic DNA
Maximum Column Loading Volume
650 μL
Size of RNA Purified
All sizes, including small RNA
(< 200 nt)
Maximum Amount of Starting Material:
Plant Tissues
Plant Cells
100 mg
5 x 106
Time to Complete 10 Purifications
30 minutes
Average Yields*
Peach Leaves (100 mg)
40 μg RNA, 5 μg gDNA

* Yield will vary depending on the type of sample processed

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.


Kit Components
Component Cat. 24400 (50 preps)
Lysis Buffer M 40 mL
Binding Buffer I 7 mL
Wash Solution A 38 mL
Elution Buffer E 20 mL
Enzyme Incubation Buffer B 6 mL
Filter Columns 50
Spin Columns 50
Collection Tubes 100
Elution Tubes (1.7 mL) 50
Product Insert 1

Title Characterization of Citrus-Associated Alternaria Species in Mediterranean Areas
Journal PLOS ONE. 2016.
Authors Garganese F, Schena L, Siciliano I, Prigigallo MI, Spadaro D, De Grassi A, Ippolito A, Sanzani SM
Title A highly effective and versatile technology for the isolation of RNAs from grapevines and other woody perennials for use in virus diagnostics
Journal Journal of Plant Biotechnology. 2015.
Authors Huogen Xiao, Won-Sik Kim and Baozhong Meng.
Title Enhanced biosynthesis of bioactive abietane diterpenes by overexpressing AtDXS or AtDXR genes in Salvia sclarea hairy roots.
Journal Journal of Plant Biotechnology. 2014.
Authors Mariacarmela Vaccaro, Nicola Malafronte, Mariaevelina Alfieri, Nunziatina De Tommasi, Antonietta Leone.
Title Non-transgenic genome modification in plant cells
Journal Plant Physiology. 2010.
Authors Ira M, Zuker A, Shklarman E, Zeevi V, Tovkach A, Roffe S, Ovadis M, Tzfira T, Vainstein A.