For simultaneous isolation of total RNA and DNA from the same plant sample
|Plant RNA/DNA Purification Kit||24400||50 preps|
Plant RNA/DNA Purification Kit
This kit provides for rapid spin column isolation and purification of total RNA and genomic DNA simultaneously from a single plant sample without splitting the lyate. Norgen's plant lysis solution is highly robust and effective over a wide range of plants including challenging samples. The total RNA and genomic DNA are both column purified in under 30 minutes. Since RNA and DNA are isolated without splitting the lysate, variability and inconsistent results are reduced. All sizes of RNA including microRNA are recovered without the need for phenol. Optional on-column DNase and RNase treatments provide flexibility to isolate DNA-free RNA or RNA-free DNA respectively. Isolated nucleic acids are of a high quality and yield, and are ready for downstream use including PCR, qPCR, RT-PCR, qRT-PCR, sequencing and more.
It is often necessary to isolate total RNA and genomic DNA from a single plant sample, such as for studies of gene expression, mutant or transgenic plant characterization, and host plant-pathogen characterization. This is of great benefit when isolating RNA and DNA from precious, difficult to obtain or very small samples. Furthermore, gene expression analysis will be more reliable since the RNA and DNA are derived from the same sample, therefore eliminating inconsistent results.
|Column Binding Capacity||
50 μg for RNA
15 μg for genomic DNA
|Maximum Column Loading Volume||
|Size of RNA Purified||
All sizes, including small RNA
(< 200 nt)
|Maximum Amount of Starting Material:
5 x 106
|Time to Complete 10 Purifications||
Peach Leaves (100 mg)
40 μg RNA, 5 μg gDNA
* Yield will vary depending on the type of sample processed
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
|Title||Characterization of Citrus-Associated Alternaria Species in Mediterranean Areas|
|Journal||PLOS ONE. 2016.|
|Authors||Garganese F, Schena L, Siciliano I, Prigigallo MI, Spadaro D, De Grassi A, Ippolito A, Sanzani SM|
|Title||A highly effective and versatile technology for the isolation of RNAs from grapevines and other woody perennials for use in virus diagnostics|
|Journal||Journal of Plant Biotechnology. 2015.|
|Authors||Huogen Xiao, Won-Sik Kim and Baozhong Meng.|
|Title||Enhanced biosynthesis of bioactive abietane diterpenes by overexpressing AtDXS or AtDXR genes in Salvia sclarea hairy roots.|
|Journal||Journal of Plant Biotechnology. 2014.|
|Authors||Mariacarmela Vaccaro, Nicola Malafronte, Mariaevelina Alfieri, Nunziatina De Tommasi, Antonietta Leone.|
|Title||Non-transgenic genome modification in plant cells|
|Journal||Plant Physiology. 2010.|
|Authors||Ira M, Zuker A, Shklarman E, Zeevi V, Tovkach A, Roffe S, Ovadis M, Tzfira T, Vainstein A.|
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