Plant/Fungi DNA Isolation Kits
For rapid isolation of total DNA from plants and fungi
For research use only and NOT intended for in vitro diagnostics.
Powered by Bioz Plant/Fungi DNA Isolation Kits
For rapid isolation of total DNA from plants and fungi
Overview
- Rapid and simple procedure
- Excellent quality and yield of DNA
- Process a broad spectrum of plant species and filamentous fungi
- Isolate total DNA including pathogen DNA without phenol
- Available in spin column format and 96-well format for high throughput applications
These kits provide a rapid method for the isolation and purification of total DNA from a wide range of plant and fungal species. Total DNA, including genomic DNA, mitochondrial DNA and chloroplast DNA can be purified from fresh or frozen plant tissues, plant cells or fungi samples using this kit. Purified DNA samples can be used for the detection of viral pathogens, as viral DNA is isolated with the plant/fungi DNA. The purified DNA is of the highest integrity, and can be used in a number of downstream applications including PCR, qPCR, SNP, Southern blotting and sequencing.
Plant/Fungi DNA Isolation Kit (Spin Column)
Complete 10 purifications in 45 minutes. This kit offers a maximum loading volume of 650 μL per column, and a maximum binding capacity of 50 μg per column.
Plant/Fungi DNA Isolation 96-Well Kit (High Throughput)
For high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system. Complete 96 purifications in 50 minutes. This kit offers a maximum loading volume of 500 μL per well, and a maximum binding capacity of 50 μg per well.
Plant/Fungi DNA Isolation Kit (Magnetic Bead System)
The DNA is bound to the surface of the magnetic beads under optimized buffer conditions and released using a low salt buffer system. The Plant DNA Isolation Kit (Magnetic Bead System) can be easily adapted to automated magnetic bead separation instruments and work stations.
Plant/Fungi DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)
For high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.
Details
Supporting Data
Figure 1. Isolate DNA from a Wide Range of Plants. DNA was isolated from 50 mg samples of tobacco leaves (Lanes 1 and 2), tomato leaves (lanes 3 and 4), peach leaves (Lanes 5 and 6) and grape leaves (lanes 7 and 8) using Norgene’s Plant/Fungi DNA Isolation Kit, and 5 µL aliquots of the 100 µL elutions were run on a 1x TAE 1% agarose gel. As it can be seen, high quality DNA was isolated in all cases. The M lanes contain Norgen’s HighRanger 1Kb DNA Ladder.
Figure 2. Purified DNA can be Amplified in a Real-time PCR Reaction (SYBR Green). DNA was isolated from 50 mg samples of plum and tobacco frozen leaves using Norgen’s Plant/Fungi DNA isolation Kit, and 2 µL of the eluted DNA was used in a real-time PCR reaction (total reaction volume 20 µL) with 18sr DNA primers (95°C for 3 minutes and 40 cycles at 95°C for 15 seconds and 60°C for 30 seconds). The real-time PCR was successful in amplifying both the plum and tobacco DNA, indicating that the DNA is of a high quality and can be used in sensitive downstream applications.
Figure 3. Resolution of DNA isolated from four different plant species. DNA was isolated from four different plant species using Norgen’s Plant DNA Isolation Kit (Magnetic Bead System) and Norgen's Plant/Fungi DNA Isolation Kit (column format, Cat. 26200). For evaluation, 10 µL from 75 µL of elution were run on 1X TAE 1.2% agarose gel. Excellent DNA integrity and yield were observed from the Plant DNA Isolation Kit (Magnetic Bead System) (Lanes 1 to 3), indicating the robust performance comparable to the column based method (Red C). Marker = Norgen’s HighRanger DNA Ladder./p>
Figure 4. High DNA Quality. DNA was isolated from a number of plant species using Norgen's Plant DNA Isolation Kit (Magnetic Bead System) and Norgen's Plant/Fungi DNA Isolation Kit (column format, Cat. 26200). The purified DNA was then compared for DNA quality (A260/280). All DNA elutions isolated using Norgen’s Plant DNA Isolation Kit (Magnetic Bead System) showed an excellent comparable DNA quality to Norgen’s Plant/Fungi DNA Isolation Kit (column based, Cat. 26200), indicating the consistent and robust performance of the Plant DNA Isolation Kit (Magnetic Bead System).
Figure 5. High DNA quality was confirmed by Real-time PCR. DNA was isolated from six different plant species using Norgen's Plan DNA Isolation Kit (Magnetic Bead System) and Norgen's Plant/Fungi DNA Isolation Kit (column format, Cat. 26200). To assess the quality of the purified DNA, 8 µL of the elution (total PCR reaction volume was 20 µL) was used in a real-time PCR reaction. As it can be seen, 18s rDNA amplification was successful in all cases without PCR inhibition, indicating the excellent quality of DNA purified using Norgen’s Plant DNA Isolation Kit (Magnetic Bead System).
Figure 6. DNA was Isolated From Plant Tissue Using Norgen’s Plant DNA Isolation 96-Well Kit (Magnetic Bead System). For evaluation, 10 µL from 75 µL of elution were run on 1X TAE 1.2% agarose gel. Excellent DNA integrity, consistency, and yield were observed from the Plant DNA Isolation 96-Well Kit (Magnetic Bead System). Marker = Norgen’s HighRanger DNA Ladder (Cat. 11900).
Figure 7. High DNA Quality Confirmed By Real-time PCR. DNA was isolated from plant tissue species using Norgen's Plant DNA Isolation 96-Well Kit (Magnetic Bead System). To assess the quality of the purified DNA, 2 µL of the elution (total PCR reaction volume was 20 µL) was used in a real-time PCR reaction. As it can be seen, 18s rDNA amplification was successful in all cases without PCR inhibition, indicating the excellent quality of DNA purified using Norgen’s Plant DNA Isolation 96-Well Kit (Magnetic Bead System).
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Kit Specifications
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Maximum Plant Input
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50 mg
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Type of Plants Processed
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All types of plant species and tissue
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Average Yield from 50 mg of Plant*
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3-10 μg
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| Average Purity (OD260/280) |
1.8 - 2.0
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| Time to Complete 12 Purifications |
50 minutes
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* Average DNA yield will vary depending on the donor
Storage Conditions and Product Stability
Store RNAse A at -20°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
| Component | Cat. 26200 (50 preps) | Cat. 26250 (250 preps) | Cat. 26900 (192 preps) | Cat. 58200 (50 preps) | Cat. 62400 (192 preps) |
|---|---|---|---|---|---|
| Lysis Buffer L | 30 mL | 1 x 30 mL 2 x 60 mL |
2 x 60 mL | 60 mL | 2 x 60 mL |
| Binding Buffer I | 7 mL | 1 x 7 mL 1 x 25 mL |
25 mL | 7 mL | 25 mL |
| Solution WN | 18 mL | 1 x 18 mL 1 x 55 mL |
55 mL | 18 mL | 55 mL |
| Wash Solution A | 38 mL | 2 x 38 mL | 2 x 38 mL | - | - |
| Elution Buffer B | 15 mL | 30 mL | 30 mL | 8 mL | 30 mL |
| RNAse A | 1 vial (80 μL) | 5 vials (5 x 80 μL) | 1 vial | 1 vial | 1 vial |
| Magnetic Bead Suspension | - | - | - | 4 x 1.1 mL | 2 x 8.5 mL |
| Filter Columns | 50 | 250 | - | - | - |
| Spin Columns | 50 | 250 | - | - | - |
| Collection Tubes | 100 | 500 | - | - | - |
| 96-Well Plate | - | - | 2 | - | 2 |
| 96-Well Collection Plate | - | - | 2 | - | - |
| Adhesive Tape | - | - | 4 | - | 2 |
| Elution Tubes (1.7 mL) | 50 | 250 | - | 50 | - |
| 96-Well Elution Plate | - | - | 2 | - | 2 |
| Product Insert | 1 | 1 | 1 | 1 | 1 |
Documentation
(26250) Plant/Fungi DNA Isolation Kit (Spin Column) - Protocol (250 Preps)
(26900) Plant/Fungi DNA Isolation 96 Well Kit (High Throughput) - Protocol (96-well)
(58200) Plant DNA Isolation Kit (Magnetic Bead System) - Protocol (50 preps)
(62400) Plant DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System) - Protocol (2 x 96-well)
A Novel Technology to Extract Nucleic Acids from Different Environmental Samples including Plants, Water and Soil
Compatibility of DNA and RNA Extraction Methods for Challenging Plant Species
FAQs
Spin Column, High Throughput, Magnetic Bead System, High Throughput Magnetic Bead System
Poor DNA recovery could be due to one or more of the following:
- Columns/wells have become clogged (Spin column or 96-well plate format).
Do not exceed the recommended amounts of starting materials. The amount of starting material may need to be decreased if the column shows clogging below the recommended levels. See also “Clogged Column” below.
- An alternative elution buffer was used.
It is recommended that the Elution Buffer B supplied with this kit be used for maximum DNA recovery.
- Ethanol was not added to the lysate.
Ensure that the indicated amount of ethanol (70% or 96-100% as mentioned in the protocol) is added to the lysate before binding to the columns/wells or introduction of magnetic bead suspension.
- Ethanol was not added to the Wash Solution.
Ensure the indicated 96-100% ethanol amount is added to the supplied Solution WN and Wash Solution A prior to use.
- This kit has 2 columns (clear - filter columns; grey - most likely silica).
Please make sure the columns were not interchanged. If that happens, then all DNA would be lost in the first step.
Column clogging may be due to one or more of the following reasons:
- Maximum amount of tissue exceeds kit specifications.
The optimal input of plant tissue or fungi (wet weight) for each kit has been described under Kit specifications and also in the product insert.
- Too much cell debris in the lysate supernatant.
The lysate should be centrifuged at 20,000 x g (~14,000 RPM) for 5 minutes and the clean supernatant should be used to proceed.
- Centrifuge temperature is too low.
Ensure that the centrifuge remains at room temperature throughout the procedure. Temperatures below 20°C may cause precipitates to form that can cause the columns to clog.
If the DNA does not perform well in downstream applications, it may be due to one or more of the following:
- PCR reaction conditions need to be optimized.
Take steps to optimize the PCR conditions being used, including varying the amount of DNA template, changing the source of Taq polymerase, looking into the primer design, and adjusting the annealing condition.
- Binding Buffer I was not added to the lysate.
Ensure that the Binding Buffer I is added to the lysate and that it is incubated on ice for 5 minutes prior to spinning down the lysate.
- DNA was not washed as directed in the kit manual.
Traces of salt from the binding step may remain in the sample if the column is not washed as directed in the kit manual. Salt may interfere with downstream applications, and thus must be washed from the column/wells/beads.
- Ethanol carryover.
Ensure that the drying step is performed after the wash steps in order to remove traces of ethanol prior to elution. Ethanol is known to interfere with many downstream applications.
RNA can get co-eluted with DNA. Carry out a digestion with RNase A on the elution if the RNA present will interfere with downstream applications. Refer to manufacturer’s instructions regarding amount of enzyme to use, optimal incubation time and temperature.
Yes, the DNA from this kit is good for long-read-sequencing.
Yes, it is possible to isolate DNA from bone tissue using this kit after decalcification of the tissue. Please feel free to contact our Tech support team at support@norgenbiotek.com for help with decalcification protocol.
Citations
Spin Column, High Throughput (26200, 26250, 26900)
| Title | Leaf Bacteriome in Sugar Beet Shows Differential Response against Beet curly top virus during Resistant and Susceptible Interactions |
| Journal | Int. J. Mol. Sci . 2022 |
| Authors | Rajtilak Majumdar, Carl A. Strausbaugh, Eric D. Vincill, Imad Eujayl and Paul J. Galewski |
| Title | Chitosan Sensitivity of Fungi Isolated from Mango (Mangifera indica L.) with Anthracnose |
| Journal | Molecules . 2022 |
| Authors | Griselda Valenzuela-Ortiz, Soila Maribel Gaxiola-Camacho, Cesar San-Martín-Hernández, Miguel Ángel Martínez-Téllez, Emmanuel Aispuro-Hernández, Jaime Lizardi-Mendoza and Eber Addí Quintana-Obregón |
| Title | L-Asparaginase from Penicillium sizovae Produced by a Recombinant Komagataella phaffii Strain |
| Journal | Pharmaceuticals . 2022 |
| Authors | Marcela Freitas, Paula Souza, Mauricio Homem-de-Mello, Yris M. Fonseca-Bazzo, Damaris Silveira, Edivaldo X. Ferreira Filho, Adalberto Pessoa Junior, Dipak Sarker, David Timson, João Inácio and Pérola O. Magalhães |
| Title | Agaricus macrochlamys, a New Species from the (Sub)tropical Cloud Forests of North America and the Caribbean, and Agaricus fiardii, a New Synonym of Agaricus subrufescens |
| Journal | J. Fungi . 2022 |
| Authors | Rosario Medel-Ortiz, Roberto Garibay-Orijel, Andrés Argüelles-Moyao, Gerardo Mata, Richard W. Kerrigan, Alan E. Bessette, József Geml, Claudio Angelini, Luis A. Parra and Jie Chen |
| Title | Marker assisted selection (MAS) for downy mildew resistance in grapevines using Rpv3.1 associated markers |
| Journal | Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2022 |
| Authors | Murat AKKURT, Irem ŞENSES, Burak AKTÜRK, Ilhami TOZLU, Ilhami TOZLU and Halil Ibrahim UZUN |
| Title | New Antifungal Compound, 6-Pentyl-α-Pyrone, against the Maize Late Wilt Pathogen, Magnaporthiopsis maydis |
| Journal | Agronomy. 2022 |
| Authors | Ofir Degani, and Asaf Gordani |
| Title | Genomic analyses point to a low evolutionary potential of prospective source populations for assisted migration in a forest herb |
| Journal | Evolutionary Applications. 2022 |
| Authors | Frederik Van Daele,Olivier Honnay,Hanne De Kort |
| Title | Common facultative endosymbionts do not influence sensitivity of cereal aphids to pyrethroids |
| Journal | RES JOUNRALS. 2022 |
| Authors | Daniel J. Leybourne, Petra Melloh, Emily A. Martin |
| Title | Emergence and Genomic Characterization of Multidrug Resistant Candida auris in Nigeria, West Africa |
| Journal | J. Fungi. 2022 |
| Authors | Rita Oladele, Jessica N. Uwanibe, Idowu B. Olawoye, Abdul-Wahab O. Ettu, Jacques F. Meis and Christian T. Happi |
| Title | Alternaria species causing pomegranate and citrus fruit rots in Albania |
| Journal | Journal of Plant Diseases and Protection. 2022 |
| Authors | Magdalena Cara, Mirela Toska, Dajana Frasheri, Riccardo Baroncelli & Simona Marianna Sanzani |
| Title | Evaluation of Three Forms of Rhizoctonia solani Mediated Pathogenicity to Sugar Beet Cultivars in Greenhouse Studies |
| Journal | bioRxiv. 2022 |
| Authors | Md Ehsanul Haque, Most Shanaj Parvin |
| Title | Phylogeographic patterns in cryptic Bostrychia tenella species (Rhodomelaceae, Rhodophyta) across the Thai-Malay Peninsula |
| Journal | Algae. 2022 |
| Authors | Jakaphan Bulan, Sinchai Maneekat, Giuseppe C. Zuccarello, Narongrit Muangmai |
| Title | Metabarcoding assessment of fungal diversity in brown algae and sponges of Mauritius |
| Journal | Frontiers in Microbiology. 2022 |
| Authors | Jessica Mélanie Wong Chin, Daneshwar Puchooa, Theeshan Bahorun, Vidushi S. Neergheen, Aadil Ahmad Aullybux, Girish Beedessee, Nadeem Nazurally, Abdulwahed Fahad Alrefaei and Rajesh Jeewon |
| Title | Phosphate solubilization potential of indigenous rhizosphere fungi and their biofertilizer formulations |
| Journal | Agronomy Research. 2021 |
| Authors | D.A. Balogun, M.A. Oke, L. Rocha-Meneses, OB. Fawole and P.F. Omojasola |
| Title | First Report of Sclerotinia sclerotiorum (Sclerotinia Blight) on Symphyotrichum dumosum in Poland |
| Journal | APS Publications. 2018 |
| Authors | K. Pieczul |
| Title | Cultivar-specific gene modulation in Vitis vinifera: analysis of the promoters regulating the expression of WOX transcription factors |
| Journal | Scientific Reports. 2017 |
| Authors | Boccacci P, Mela A, Pavez Mina C, Chitarra W, Perrone I, Gribaudo I, Gambino G. |
| Title | Lotus japonicus NF-YA1 plays an essential role during nodule differentiation and targets members of the SHI/STY gene family |
| Journal | Molecular plant-microbe interactions: MPMI. 2016. |
| Authors | Hossain MS, Shrestha A, Zhong S, Miri M, Austin RS, Sato S, Ross L, Huebert T, Tromas A, Torres-Jerez I, Tang Y, Udvardi M, Murray JD, Szczyglowski K. |
| Title | Numerical and RAPD Analysis of Eight Cowpea Genotypes from Nigeria |
| Journal | Nigerian Journal of Biotechnology. 2016. |
| Authors | E.O. Idehen, S.A. Oyelakin, A.O. Onikola, V.O. Adedapo |
| Title | Chemical Control of Black Scorch Disease on Date Palm Caused by the Fungal Pathogen, Thielaviopsis punctulata in United Arab Emirates |
| Journal | Plant Disease. 2016. |
| Authors | Esam Eldin Saeed, Arjun Sham, Khaled Abbas El-Tarabily, Firas Abu Elsamen, Rabah Iratni, Synan F. AbuQamar |
| Title | Identification Of Cuscuta Campestrisyuncker In Uae: Study Of Bar Code Loci-Rbcl, Matk And Trnh-Psbain The Uae And Egyptian Cultivars And In The Respective Host Plants Basil And Jute |
| Journal | International Journal of Research in Science. 2016. |
| Authors | Alsaadi, A., Saif, M., Yasser, M., Gaur, S., El-wakil, H., Maloukh, L., & Lakshmi, T. V. R |
| Title | Potential biosurfactant producing endophytic and epiphytic fungi, isolated from macrophytes in the Negro River in Manaus, Amazonas, Brazil |
| Journal | African Journal of Biotechnology. 2016. |
| Authors | Lima, J. M. S., Pereira, J. E. O., Batista, I. H., Neto, P. D. Q. C., dos Santos, J. C., de Ara&ujo, S. P., ... & de Azevedo, J. L. U |
| Title | Fate of transgenic deoxyribonucleic acid fragments in digesta and tissues of rabbits fed genetically modified soybean meal |
| Journal | Journal of Animal Science. 2015. |
| Authors | P. Morera, L. Basiricò, B. Ronchi and U. Bernabucci |
| Title | MiRNA-Mediated Regulation of the SWI/SNF Chromatin Remodeling Complex Controls Pluripotency and Endodermal Differentiation in Human ESCs |
| Journal | Stem Cells. 2015. |
| Authors | Wade SL, Langer LF, Ward JM, Archer TK |
| Title | Mangrove Rhizosphere Soils: A Unique Natural Source of Pravastatin-Producing Penicillium Microfungi |
| Journal | International Journal of Extensive Research. 2015. |
| Authors | Seydametova Emine, Norazwina Zainol, Jailani Salihon and Peter Convey |
| Title | A stable JAZ protein from peach mediates the transition from outcrossing to self-pollination |
| Journal | BMC Biology. 2015. |
| Authors | Sherif Sherif, Islam El-Sharkawy, Jaideep Mathur, Pratibha Ravindran, Prakash Kumar, Gopinadhan Paliyath and Subramanian Jayasankar |
| Title | Genome and Metagenome Sequencing: Using the Human Methyl-Binding Domain to Partition Genomic DNA Derived from Plant Tissues. |
| Journal | Applications in Plant Sciences. 2014. |
| Authors | Erbay Yigit, David I. Hernandez, Joshua T. Trujillo, Eileen Dimalanta, and C. Donovan Bailey. |
| Title | Establishment of invasive and non-invasive reporter systems to investigate American elm–Ophiostoma novo-ulmi interactions. |
| Journal | Fungal Genetics and Biology. 2014. |
| Authors | S. Sherif, A.M.P. Jones, M.R. Shukla, P.K. Saxena. |
| Title | Aplification of TRP1 for Specific Detection of Phytophthora Ramorum. |
| Journal | United States Patent Application 022057. 2014. |
| Authors | Bohannon, Robert C., Russell Jr., Paul F. |
| Title | Development of a Real-Time Microchip PCR System for Portable Plant Disease Diagnosis. |
| Journal | PLoS One. 2013. |
| Authors | Koo C, Malapi-Wight M, Kim H, Cifci O, Vaughn-Diaz V, Ma B, Kim S, Abdel-Raziq H, Ong K, Jo Y, Gross D, Shim W, Han A |
| Title | Optimization of decolorization of palm oil mill effluent (POME) by growing cultures of Aspergillus fumigatus using response surface methodology. |
| Journal | Environmental Science and Pollution Research. 2012. |
| Authors | Neoh C, Yahya A, Adnan R, Majid Z and Ibrahim Z. |
| Title | Three sympatrically occurring species of Metarhizium show plant rhizosphere specificity. |
| Journal | Journal of Microbiology. 2011. |
| Authors | Wyrebek M, Huber C, Sasan RK, Bidochka MJ. |
| Title | Molecular cloning and expression analysis of a delta 6-fatty acid desaturase gene from Rhizopus stolonifer strain YF6 which can accumulate high levels of gamma-linolenic acid. |
| Journal | Journal of Microbiology. 2011. |
| Authors | Wan X, Zhang Y, Wang P, Jiang M. |
| Title | Flexible metabolism in Metarhizium anisopliae and Beauveria bassiana: utility of the glyoxylate cycle during insect pathogenesis |
| Journal | Microbiology. 2011. |
| Authors | Padilla-Guerrero IE, Barelli L, Gonzˆlez-Hernˆndez GA, Torres-Guzmˆn JC, Bidochka M. |
