Plasma/Serum Cell-Free Circulating DNA Purification Kits

Cat. 55500, 55100, 55600, 55800
  • Professional Tech Support with Senior R&D Scientist
  • Fast and Flexible Ordering Options (online, email, or phone)
  • Volume Discounts Available on Large Quantity Orders*

*Inquire by phone or email for more information.


Plasma/Serum Cell-Free Circulating DNA Purification Kits

Cat. 55500, 55100, 55600, 55800

For rapid and simple purification of cell-free circulating DNA from plasma/serum samples

  • Isolate all sizes of circulating DNA from plasma and serum samples
  • Isolate viral and bacterial DNA
  • Versatile plasma and serum input volumes
  • Concentrate circulating DNA into a flexible elution volume
  • Isolate inhibitor-free cell-free circulating DNA
  • Compatible with Streck Cell-Free DNA BCT® Tubes
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

Want a discount?

Create your account to receive 15% off on your first purchase!

Plasma/Serum Cell-Free Circulating DNA Purification Micro Kit
(Cat. 55500)
50 preps
Plasma/Serum Cell-Free Circulating DNA Purification Mini Kit
(Cat. 55100)
50 preps
Plasma/Serum Cell-Free Circulating DNA Purification Midi Kit
(Cat. 55600)
20 preps
Plasma/Serum Cell-Free Circulating DNA Purification Maxi Kit
(Cat. 55800)
10 preps

Kit Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Kit Size

  • Isolate all sizes of circulating DNA from plasma and serum samples
  • Isolate viral and bacterial DNA
  • Versatile plasma and serum input volumes
  • Concentrate circulating DNA into a flexible elution volume
  • Isolate inhibitor-free cell-free circulating DNA
  • Compatible with Streck Cell-Free DNA BCT® Tubes
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Plasma/Serum Cell-Free Circulating DNA Purification Kits

These kits provide a fast, reliable and convenient spin column method for the isolation of high quality, high purity and inhibitor-free cell-free circulating DNA (cfc-DNA) from plasma/serum sample volumes. The purified plasma/serum cfc-DNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays and NGS.

Background

Plasma/Serum cell-free circulating DNA (cfc-DNA) has the potential to provide biomarkers for certain cancers and disease states as well as fetal DNA in maternal blood. Currently, significant advancements are being made in utilizing cfc-DNA as biomarkers for the early diagnosis, prognosis and monitoring of therapy for several cancer types and autoimmune diseases. Cell-free mitochondrial DNA (cfmtDNA) is also under investigation for its clinical significance. This cfc-DNA is usually present as short fragments of less than 1000 bp. In addition, cell-free fetal DNA has been widely used as a non-invasive method for prenatal diagnosis including early identification of fetal sex, genetic studies for families at high risk for inherited genetic disorders, screening for Rhesus factor, screening for aneuploidy and identification of preeclampsia.

Plasma/Serum Cell-Free Circulating DNA Purification Micro Kit

Ideal for handling initial plasma/serum volumes ranging from 10 μL up to 200 μL. The kit is designed to isolate all sizes of cfc-DNA from either fresh or frozen plasma/serum samples and the purified DNA is eluted into a flexible elution volume ranging from 25 µL to 50 µL.

Plasma/Serum Cell-Free Circulating DNA Purification Mini Kit

Ideal for handling initial plasma/serum volumes ranging from 200 μL up to 500 μL. The kit is designed to isolate all sizes of cfc-DNA from either fresh or frozen plasma/serum samples and the purified DNA is eluted into a flexible elution volume ranging from 25 µL to 50 µL.

Plasma/Serum Cell-Free Circulating DNA Purification Midi Kit

Ideal for handling initial plasma/serum volumes ranging from 1 mL - 4 mL. The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 25 µL to 50 µL

Plasma/Serum Cell-Free Circulating DNA Purification Maxi Kit

Ideal for handling initial plasma/serum volumes ranging from 5 mL- 10 mL. The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 25 µL to 50 µL

Kit Specifications

You have selected: Cat. 55500
Kit Specifications
Minimum Plasma/Serum Input 10 μL
Maximum Plasma/Serum Input 200 μL
Size of DNA Purified ≥ 50 bp
Elution Volume 25-50 μL
Time to Complete 10 Purifications 15-20 minutes
Average Yields* Variable depending on specimen

*Please check page 7 in the product manual for the Plasma/Serum Average Yields and the Common DNA Quantification Methods.

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.

You have selected: Cat. 55100
Kit Specifications
Minimum Plasma/Serum Input
200 μL
Maximum Plasma/Serum Input
500 μL
Size of DNA Purified
≥ 50 bp
Elution Volume
25-50 μL
Time to Complete 10 Purifications
15-20 minutes
Average Yields
Variable depending on specimen

*Please check page 7 in the product manual for the Plasma/Serum Average Yields and the Common DNA Quantification Methods.

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.

You have selected: Cat. 55600
Kit Specifications
Minimum Plasma/Serum Input 1 mL
Maximum Plasma/Serum Input 4 mL
Size of DNA Purified ≥ 50 bp
Elution Volume 25-50 μL
Time to Complete 10 Purifications 90 minutes
Average Yields* Variable depending on specimen

*Please check page 7 in the product manual for the Plasma/Serum Average Yields and the Common DNA Quantification Methods.

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer.

All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.

You have selected: Cat. 55800
Kit Specifications
Minimum Plasma/Serum Input 5 mL
Maximum Plasma/Serum Input 10 mL
Size of DNA Purified All sizes
Elution Volume 25-50 μL
Time to Complete 10 Purifications 90 minutes
Average Yields* Variable depending on specimen

*Please check page 7 in the product manual for the Plasma/Serum Average Yields and the Common DNA Quantification Methods.

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.

Supporting Data

Click for expanded view

Component Cat. 55500 (50 preps) Cat. 55100 (50 preps) Cat. 55600 (20 preps) Cat. 55800 (10 preps)
Binding Buffer B 40 mL 2 x 40 mL 2 x 85 mL
1 x 12 mL
2 x 85 mL
1 x 40 mL
Proteinase K 0.6 mL 2 x 1 mL 6.5 mL 8 mL
Solution WN 18 mL 18 mL 18 mL 18 mL
Wash Solution A 18 mL 38 mL 18 mL 18 mL
Elution Buffer B 8 mL 8 mL 30 mL 30 mL
Micro Spin Columns 50 50 20 10
Mini Spin Columns - 50 - -
Midi Spin Columns - - 20 -
Maxi Spin Columns - - - 10
Collection Tubes 50 100 20 10
Elution Tubes (1.7 mL) 50 100 20 10
Product Insert 1 1 1 1

Midi (55500)

Title Aqueous humor as a surrogate biomarker for retinoblastoma tumor tissue
Journal Journal of American Association for Pediatric Ophthalmology and Strabismus. 2022.
Authors Vishal Raval MD, Hilary Racher PhD, Jacquelyn Wrenn MSN, APRN-CNP, Arun D.Singh MD
Title Evaluation of commercial kits for purification of circulating free DNA
Journal Cancer Genetics. 2019.
Authors Russell J. Diefenbach, Jenny H. Lee, Richard F. Kefford, Helen Rizos,
Title Enzyme-Powered Three-Dimensional DNA Nanomachine for DNA Walking, Payload Release, and Biosensing
Journal ACS Nano. 2016.
Authors Yang, X., Tang, Y., Mason, S. D., Chen, J., & Li, F

Mini (55100)

Title Early detection of colorectal cancer somatic mutations using cfDNA liquid biopsies in a murine carcinogenesis model
Journal Journal of Cancer. 2022.
Authors Héctor Martínez-Gregorio, Clara Estela Díaz-Velásquez, Mario Esaú Romero-Piña, Miguel Ruiz De La Cruz, Norma Laura Delgado-Buenrostro, Aldo De La Cruz-Montoya, Yolanda Irasema Chirino, Luis Ignacio Terrazas, Luis Alberto Medina, Felipe Vaca-Paniagua
Title A multiparametric extraction method for Vn96-isolated plasma extracellular vesicles and cell-free DNA that enables multi-omic profiling
Journal Scientific Reports. 2021.
Authors Jeremy W. Roy, Catherine A. Taylor, Annie P. Beauregard, Surendar R. Dhadi, D. Craig Ayre, Sheena Fry, Simi Chacko, Gabriel Wajnberg, Andrew P. Joy, Ngoc-Nu Mai-Thi, Nicolas Crapoulet, David A. Barnett, Anirban Ghosh, Stephen M. Lewis & Rodney J. Ouellette
Title Evaluation of commercial kits for purification of circulating free DNA
Journal Cancer Genetics. 2019.
Authors Russell J. Diefenbach, Jenny H. Lee, Richard F. Kefford, Helen Rizos,
Title 554P KRAS-dependent and independent mechanisms of progressive disease (PD) in colorectal cancer (CRC) patients (pts) with liver metastases (LM) while monitoring on circulating cell free DNA (cfDNA)
Journal Annals of Oncology. October 23, 2018.
Authors A Kirov Z Mihaylova, V Petrova, T Todorov, D Petkova, A Garev, A Todorova-Georgieva
Title Advances in Circulating Tumor DNA Analysis
Journal Advances in Clinical Chemistry. 2017.
Authors S. Perakis, M. Auer, J. Belic, E. Heitzer
Title Comprehensive evaluation of methods to isolate, quantify, and characterize circulating cell-free DNA from small volumes of plasma
Journal Analytical and Bioanalytical Chemistry. 2015.
Authors Mauger F, Dulary C, Daviaud C, Deleuze JF, Tost J.

Maxi (55600)

Title Evaluation of commercial kits for purification of circulating free DNA
Journal Cancer Genetics. 2019.
Authors Russell J. Diefenbach, Jenny H. Lee, Richard F. Kefford, Helen Rizos,

Maxi (55800)

Title Evaluation of commercial kits for purification of circulating free DNA
Journal Cancer Genetics. 2019.
Authors Russell J. Diefenbach, Jenny H. Lee, Richard F. Kefford, Helen Rizos,

Plasma/Serum Cell-Free Circulating DNA Purification Kits

These kits provide a fast, reliable and convenient spin column method for the isolation of high quality, high purity and inhibitor-free cell-free circulating DNA (cfc-DNA) from plasma/serum sample volumes. The purified plasma/serum cfc-DNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays and NGS.

Background

Plasma/Serum cell-free circulating DNA (cfc-DNA) has the potential to provide biomarkers for certain cancers and disease states as well as fetal DNA in maternal blood. Currently, significant advancements are being made in utilizing cfc-DNA as biomarkers for the early diagnosis, prognosis and monitoring of therapy for several cancer types and autoimmune diseases. Cell-free mitochondrial DNA (cfmtDNA) is also under investigation for its clinical significance. This cfc-DNA is usually present as short fragments of less than 1000 bp. In addition, cell-free fetal DNA has been widely used as a non-invasive method for prenatal diagnosis including early identification of fetal sex, genetic studies for families at high risk for inherited genetic disorders, screening for Rhesus factor, screening for aneuploidy and identification of preeclampsia.

Plasma/Serum Cell-Free Circulating DNA Purification Micro Kit

Ideal for handling initial plasma/serum volumes ranging from 10 μL up to 200 μL. The kit is designed to isolate all sizes of cfc-DNA from either fresh or frozen plasma/serum samples and the purified DNA is eluted into a flexible elution volume ranging from 25 µL to 50 µL.

Plasma/Serum Cell-Free Circulating DNA Purification Mini Kit

Ideal for handling initial plasma/serum volumes ranging from 200 μL up to 500 μL. The kit is designed to isolate all sizes of cfc-DNA from either fresh or frozen plasma/serum samples and the purified DNA is eluted into a flexible elution volume ranging from 25 µL to 50 µL.

Plasma/Serum Cell-Free Circulating DNA Purification Midi Kit

Ideal for handling initial plasma/serum volumes ranging from 1 mL - 4 mL. The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 25 µL to 50 µL

Plasma/Serum Cell-Free Circulating DNA Purification Maxi Kit

Ideal for handling initial plasma/serum volumes ranging from 5 mL- 10 mL. The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 25 µL to 50 µL

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 55500
Kit Specifications
Minimum Plasma/Serum Input 10 μL
Maximum Plasma/Serum Input 200 μL
Size of DNA Purified ≥ 50 bp
Elution Volume 25-50 μL
Time to Complete 10 Purifications 15-20 minutes
Average Yields* Variable depending on specimen

*Please check page 7 in the product manual for the Plasma/Serum Average Yields and the Common DNA Quantification Methods.

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.

You have selected: Cat. 55100
Kit Specifications
Minimum Plasma/Serum Input
200 μL
Maximum Plasma/Serum Input
500 μL
Size of DNA Purified
≥ 50 bp
Elution Volume
25-50 μL
Time to Complete 10 Purifications
15-20 minutes
Average Yields
Variable depending on specimen

*Please check page 7 in the product manual for the Plasma/Serum Average Yields and the Common DNA Quantification Methods.

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.

You have selected: Cat. 55600
Kit Specifications
Minimum Plasma/Serum Input 1 mL
Maximum Plasma/Serum Input 4 mL
Size of DNA Purified ≥ 50 bp
Elution Volume 25-50 μL
Time to Complete 10 Purifications 90 minutes
Average Yields* Variable depending on specimen

*Please check page 7 in the product manual for the Plasma/Serum Average Yields and the Common DNA Quantification Methods.

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer.

All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.

You have selected: Cat. 55800
Kit Specifications
Minimum Plasma/Serum Input 5 mL
Maximum Plasma/Serum Input 10 mL
Size of DNA Purified All sizes
Elution Volume 25-50 μL
Time to Complete 10 Purifications 90 minutes
Average Yields* Variable depending on specimen

*Please check page 7 in the product manual for the Plasma/Serum Average Yields and the Common DNA Quantification Methods.

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.

Component Cat. 55500 (50 preps) Cat. 55100 (50 preps) Cat. 55600 (20 preps) Cat. 55800 (10 preps)
Binding Buffer B 40 mL 2 x 40 mL 2 x 85 mL
1 x 12 mL
2 x 85 mL
1 x 40 mL
Proteinase K 0.6 mL 2 x 1 mL 6.5 mL 8 mL
Solution WN 18 mL 18 mL 18 mL 18 mL
Wash Solution A 18 mL 38 mL 18 mL 18 mL
Elution Buffer B 8 mL 8 mL 30 mL 30 mL
Micro Spin Columns 50 50 20 10
Mini Spin Columns - 50 - -
Midi Spin Columns - - 20 -
Maxi Spin Columns - - - 10
Collection Tubes 50 100 20 10
Elution Tubes (1.7 mL) 50 100 20 10
Product Insert 1 1 1 1

Midi (55500)

Title Aqueous humor as a surrogate biomarker for retinoblastoma tumor tissue
Journal Journal of American Association for Pediatric Ophthalmology and Strabismus. 2022.
Authors Vishal Raval MD, Hilary Racher PhD, Jacquelyn Wrenn MSN, APRN-CNP, Arun D.Singh MD
Title Evaluation of commercial kits for purification of circulating free DNA
Journal Cancer Genetics. 2019.
Authors Russell J. Diefenbach, Jenny H. Lee, Richard F. Kefford, Helen Rizos,
Title Enzyme-Powered Three-Dimensional DNA Nanomachine for DNA Walking, Payload Release, and Biosensing
Journal ACS Nano. 2016.
Authors Yang, X., Tang, Y., Mason, S. D., Chen, J., & Li, F

Mini (55100)

Title Early detection of colorectal cancer somatic mutations using cfDNA liquid biopsies in a murine carcinogenesis model
Journal Journal of Cancer. 2022.
Authors Héctor Martínez-Gregorio, Clara Estela Díaz-Velásquez, Mario Esaú Romero-Piña, Miguel Ruiz De La Cruz, Norma Laura Delgado-Buenrostro, Aldo De La Cruz-Montoya, Yolanda Irasema Chirino, Luis Ignacio Terrazas, Luis Alberto Medina, Felipe Vaca-Paniagua
Title A multiparametric extraction method for Vn96-isolated plasma extracellular vesicles and cell-free DNA that enables multi-omic profiling
Journal Scientific Reports. 2021.
Authors Jeremy W. Roy, Catherine A. Taylor, Annie P. Beauregard, Surendar R. Dhadi, D. Craig Ayre, Sheena Fry, Simi Chacko, Gabriel Wajnberg, Andrew P. Joy, Ngoc-Nu Mai-Thi, Nicolas Crapoulet, David A. Barnett, Anirban Ghosh, Stephen M. Lewis & Rodney J. Ouellette
Title Evaluation of commercial kits for purification of circulating free DNA
Journal Cancer Genetics. 2019.
Authors Russell J. Diefenbach, Jenny H. Lee, Richard F. Kefford, Helen Rizos,
Title 554P KRAS-dependent and independent mechanisms of progressive disease (PD) in colorectal cancer (CRC) patients (pts) with liver metastases (LM) while monitoring on circulating cell free DNA (cfDNA)
Journal Annals of Oncology. October 23, 2018.
Authors A Kirov Z Mihaylova, V Petrova, T Todorov, D Petkova, A Garev, A Todorova-Georgieva
Title Advances in Circulating Tumor DNA Analysis
Journal Advances in Clinical Chemistry. 2017.
Authors S. Perakis, M. Auer, J. Belic, E. Heitzer
Title Comprehensive evaluation of methods to isolate, quantify, and characterize circulating cell-free DNA from small volumes of plasma
Journal Analytical and Bioanalytical Chemistry. 2015.
Authors Mauger F, Dulary C, Daviaud C, Deleuze JF, Tost J.

Maxi (55600)

Title Evaluation of commercial kits for purification of circulating free DNA
Journal Cancer Genetics. 2019.
Authors Russell J. Diefenbach, Jenny H. Lee, Richard F. Kefford, Helen Rizos,

Maxi (55800)

Title Evaluation of commercial kits for purification of circulating free DNA
Journal Cancer Genetics. 2019.
Authors Russell J. Diefenbach, Jenny H. Lee, Richard F. Kefford, Helen Rizos,

 


Related Products