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Plasma/Serum Cell-Free Circulating DNA Purification Kits Dx

Cat. Dx55500, Dx55100, Dx55600, Dx55800
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Plasma/Serum Cell-Free Circulating DNA Purification Kits Dx

Cat. Dx55500, Dx55100, Dx55600, Dx55800

For isolating cell-free circulating DNA (cfc-DNA) from plasma/serum for subsequent in vitro diagnostic use

  • Concentrate circulating DNA into a flexible elution volume ranging from (25 µL - 50 µL)
  • Isolate inhibitor-free cell-free circulating DNA
  • Compatible with Streck Cell-Free DNA BCT® Tubes
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
  • Research use format is also available

For in vitro diagnostic use. Not available for sale in the United States.

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Plasma/Serum Cell-Free Circulating DNA Purification Micro Kit Dx
(Cat. Dx55500)
50 Preps
Plasma/Serum Cell-Free Circulating DNA Purification Mini Kit Dx
(Cat. Dx55100)
50 Preps
Plasma/Serum Cell-Free Circulating DNA Purification Midi Kit Dx
(Cat. Dx55600)
20 Preps
Plasma/Serum Cell-Free Circulating DNA Purification Maxi Kit Dx
(Cat. Dx55800)
10 Preps

Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

Kit Specifications

You have selected: Cat. Dx55500
Kit Specifications
Minimum Plasma/Serum Input 10 μL
Maximum Plasma/Serum Input 200 μL
Size of DNA Purified ≥ 50 bp
Elution Volume 25-50 μL
Time to Complete 10 Purifications 15-20 minutes
Average Yields* Variable depending on specimen

*Please check page 7 in the product manual for the Plasma/Serum Average Yields and the Common DNA Quantification Methods.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 years without showing any reduction in performance. Norgen's Plasma/Serum Cell-Free Circulating DNA Purification Kits contain ready-to-use Proteinase K solution, which is dissolved in a specially prepared storage buffer. The Proteinase K is stable for up to 2.5 years after delivery when stored at room temperature. To prolong the lifetime of Proteinase K, storage at 2–8°C is recommended.

You have selected: Cat. Dx55100
Kit Specifications
Minimum Plasma/Serum Input
200 μL
Maximum Plasma/Serum Input
500 μL
Size of DNA Purified
≥ 50 bp
Elution Volume
25-50 μL
Time to Complete 10 Purifications
15-20 minutes
Average Yields
Variable depending on specimen

*Please check page 7 in the product manual for the Plasma/Serum Average Yields and the Common DNA Quantification Methods.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 years without showing any reduction in performance.

Norgen's Plasma/Serum Cell-Free Circulating DNA Purification Kits contain ready-to-use Proteinase K solution, which is dissolved in a specially prepared storage buffer. The Proteinase K is stable for up to 2.5 years after delivery when stored at room temperature. To prolong the lifetime of Proteinase K, storage at 2–8°C is recommended.

You have selected: Cat. Dx55600
Kit Specifications
Minimum Plasma/Serum Input 1 mL
Maximum Plasma/Serum Input 4 mL
Size of DNA Purified ≥ 50 bp
Elution Volume 25-50 μL
Time to Complete 10 Purifications 90 minutes
Average Yields* Variable depending on specimen

*Please check page 7 in the product manual for the Plasma/Serum Average Yields and the Common DNA Quantification Methods.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 years without showing any reduction in performance.

Norgen's Plasma/Serum Cell-Free Circulating DNA Purification Kits contain ready-to-use Proteinase K solution, which is dissolved in a specially prepared storage buffer.

The Proteinase K is stable for up to 2.5 years after delivery when stored at room temperature. To prolong the lifetime of Proteinase K, storage at 2–8°C is recommended.

You have selected: Cat. Dx55800
Kit Specifications
Minimum Plasma/Serum Input 5 mL
Maximum Plasma/Serum Input 10 mL
Size of DNA Purified All sizes
Elution Volume 25-50 μL
Time to Complete 10 Purifications 90 minutes
Average Yields* Variable depending on specimen

*Please check page 7 in the product manual for the Plasma/Serum Average Yields and the Common DNA Quantification Methods.

Storage Conditions and Product Stability

All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 years without showing any reduction in performance.

Norgen's Plasma/Serum Cell-Free Circulating DNA Purification Kits contain ready-to-use Proteinase K solution, which is dissolved in a specially prepared storage buffer. The Proteinase K is stable for up to 2.5 years after delivery when stored at room temperature. To prolong the lifetime of Proteinase K, storage at 2–8°C is recommended.

Supporting Data

Click for expanded view

Micro (Dx55500)

Title Evaluation of commercial kits for purification of circulating free DNA
Journal Cancer Genetics. 2019.
Authors Russell J. Diefenbach, Jenny H. Lee, Richard F. Kefford, Helen Rizos,
Title Enzyme-Powered Three-Dimensional DNA Nanomachine for DNA Walking, Payload Release, and Biosensing
Journal ACS Nano. 2016.
Authors Yang, X., Tang, Y., Mason, S. D., Chen, J., & Li, F

Mini (Dx55100)

Title Evaluation of commercial kits for purification of circulating free DNA
Journal Cancer Genetics. 2019.
Authors Russell J. Diefenbach, Jenny H. Lee, Richard F. Kefford, Helen Rizos,
Title 554P KRAS-dependent and independent mechanisms of progressive disease (PD) in colorectal cancer (CRC) patients (pts) with liver metastases (LM) while monitoring on circulating cell free DNA (cfDNA)
Journal Annals of Oncology. October 23, 2018.
Authors A Kirov Z Mihaylova, V Petrova, T Todorov, D Petkova, A Garev, A Todorova-Georgieva
Title Advances in Circulating Tumor DNA Analysis
Journal Advances in Clinical Chemistry. 2017.
Authors S. Perakis, M. Auer, J. Belic, E. Heitzer
Title Comprehensive evaluation of methods to isolate, quantify, and characterize circulating cell-free DNA from small volumes of plasma
Journal Analytical and Bioanalytical Chemistry. 2015.
Authors Mauger F, Dulary C, Daviaud C, Deleuze JF, Tost J.

Midi (Cat. Dx55600)

Title Evaluation of commercial kits for purification of circulating free DNA
Journal Cancer Genetics. 2019.
Authors Russell J. Diefenbach, Jenny H. Lee, Richard F. Kefford, Helen Rizos,

Maxi kit (Dx55800)

Title Evaluation of commercial kits for purification of circulating free DNA
Journal Cancer Genetics. 2019.
Authors Russell J. Diefenbach, Jenny H. Lee, Richard F. Kefford, Helen Rizos,

Plasma/Serum Cell-Free Circulating DNA Purification Kits Dx

These kits provide a fast, reliable and convenient spin column method for the isolation of high quality, high purity and inhibitor-free cell-free circulating DNA (cfc-DNA) from plasma/serum sample. These kits are designed to isolate all sizes of cfc-DNA from either fresh or frozen plasma/serum samples and the purified DNA is eluted into a flexible elution volume ranging from 25 µL to 50 µL. The purified plasma/serum cfc-DNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays and NGS.

Background

Plasma/Serum cell-free circulating DNA (cfc-DNA) has the potential to provide biomarkers for certain cancers and disease states as well as fetal DNA in maternal blood. Currently, significant advancements are being made in utilizing cfc-DNA as biomarkers for the early diagnosis, prognosis and monitoring of therapy for several cancer types and autoimmune diseases. Cell-free mitocondrial DNA (cfmtDNA) is also under investigation for its clinical significance. This cfc-DNA is usually present as short fragments of less than 1000 bp. In addition, cell-free fetal DNA has been widely used as a non-invasive method for prenatal diagnosis including early identification of fetal sex, genetic studies for families at high risk for inherited genetic disorders, screening for Rhesus factor, screening for aneuploidy and identification of preeclampsia.

Plasma/Serum Cell-Free Circulating DNA Purification Micro Kit Dx

For serum input volumes 10 µL - 200 µL. Purify high-quality DNA in 15-20 minutes

Plasma/Serum Cell-Free Circulating DNA Purification Mini Kit Dx

For serum input volumes 200 µL - 500 µL. Purify high-quality DNA in 15-20 minutes.

Plasma/Serum Cell-Free Circulating DNA Purification Midi Kit Dx

The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 25 µL to 50 µL. All components for the purification & concentration are provided in one convenient & fast kit for the easy processing of large input volumes of bodily fluids. For a schematic workflow of the protocol click here to view. For serum input volumes 1 mL - 4 mL. Purify high-quality DNA in 90 minutes.

Plasma/Serum Cell-Free Circulating DNA Purification Maxi Kit Dx

The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 25 µL to 50 µL. All components for the purification & concentration are provided in one convenient & fast kit for the easy processing of large input volumes of bodily fluids. For a schematic workflow of the protocol click here to view. For serum input volumes 5 mL - 10 mL. Two kits in one - purify and concentrate DNA from bodily fluids using a convenient two column system.

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. Dx55500
Kit Specifications
Minimum Plasma/Serum Input 10 μL
Maximum Plasma/Serum Input 200 μL
Size of DNA Purified ≥ 50 bp
Elution Volume 25-50 μL
Time to Complete 10 Purifications 15-20 minutes
Average Yields* Variable depending on specimen

*Please check page 7 in the product manual for the Plasma/Serum Average Yields and the Common DNA Quantification Methods.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 years without showing any reduction in performance. Norgen's Plasma/Serum Cell-Free Circulating DNA Purification Kits contain ready-to-use Proteinase K solution, which is dissolved in a specially prepared storage buffer. The Proteinase K is stable for up to 2.5 years after delivery when stored at room temperature. To prolong the lifetime of Proteinase K, storage at 2–8°C is recommended.

You have selected: Cat. Dx55100
Kit Specifications
Minimum Plasma/Serum Input
200 μL
Maximum Plasma/Serum Input
500 μL
Size of DNA Purified
≥ 50 bp
Elution Volume
25-50 μL
Time to Complete 10 Purifications
15-20 minutes
Average Yields
Variable depending on specimen

*Please check page 7 in the product manual for the Plasma/Serum Average Yields and the Common DNA Quantification Methods.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 years without showing any reduction in performance.

Norgen's Plasma/Serum Cell-Free Circulating DNA Purification Kits contain ready-to-use Proteinase K solution, which is dissolved in a specially prepared storage buffer. The Proteinase K is stable for up to 2.5 years after delivery when stored at room temperature. To prolong the lifetime of Proteinase K, storage at 2–8°C is recommended.

You have selected: Cat. Dx55600
Kit Specifications
Minimum Plasma/Serum Input 1 mL
Maximum Plasma/Serum Input 4 mL
Size of DNA Purified ≥ 50 bp
Elution Volume 25-50 μL
Time to Complete 10 Purifications 90 minutes
Average Yields* Variable depending on specimen

*Please check page 7 in the product manual for the Plasma/Serum Average Yields and the Common DNA Quantification Methods.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 years without showing any reduction in performance.

Norgen's Plasma/Serum Cell-Free Circulating DNA Purification Kits contain ready-to-use Proteinase K solution, which is dissolved in a specially prepared storage buffer.

The Proteinase K is stable for up to 2.5 years after delivery when stored at room temperature. To prolong the lifetime of Proteinase K, storage at 2–8°C is recommended.

You have selected: Cat. Dx55800
Kit Specifications
Minimum Plasma/Serum Input 5 mL
Maximum Plasma/Serum Input 10 mL
Size of DNA Purified All sizes
Elution Volume 25-50 μL
Time to Complete 10 Purifications 90 minutes
Average Yields* Variable depending on specimen

*Please check page 7 in the product manual for the Plasma/Serum Average Yields and the Common DNA Quantification Methods.

Storage Conditions and Product Stability

All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 years without showing any reduction in performance.

Norgen's Plasma/Serum Cell-Free Circulating DNA Purification Kits contain ready-to-use Proteinase K solution, which is dissolved in a specially prepared storage buffer. The Proteinase K is stable for up to 2.5 years after delivery when stored at room temperature. To prolong the lifetime of Proteinase K, storage at 2–8°C is recommended.

Micro (Dx55500)

Title Evaluation of commercial kits for purification of circulating free DNA
Journal Cancer Genetics. 2019.
Authors Russell J. Diefenbach, Jenny H. Lee, Richard F. Kefford, Helen Rizos,
Title Enzyme-Powered Three-Dimensional DNA Nanomachine for DNA Walking, Payload Release, and Biosensing
Journal ACS Nano. 2016.
Authors Yang, X., Tang, Y., Mason, S. D., Chen, J., & Li, F

Mini (Dx55100)

Title Evaluation of commercial kits for purification of circulating free DNA
Journal Cancer Genetics. 2019.
Authors Russell J. Diefenbach, Jenny H. Lee, Richard F. Kefford, Helen Rizos,
Title 554P KRAS-dependent and independent mechanisms of progressive disease (PD) in colorectal cancer (CRC) patients (pts) with liver metastases (LM) while monitoring on circulating cell free DNA (cfDNA)
Journal Annals of Oncology. October 23, 2018.
Authors A Kirov Z Mihaylova, V Petrova, T Todorov, D Petkova, A Garev, A Todorova-Georgieva
Title Advances in Circulating Tumor DNA Analysis
Journal Advances in Clinical Chemistry. 2017.
Authors S. Perakis, M. Auer, J. Belic, E. Heitzer
Title Comprehensive evaluation of methods to isolate, quantify, and characterize circulating cell-free DNA from small volumes of plasma
Journal Analytical and Bioanalytical Chemistry. 2015.
Authors Mauger F, Dulary C, Daviaud C, Deleuze JF, Tost J.

Midi (Cat. Dx55600)

Title Evaluation of commercial kits for purification of circulating free DNA
Journal Cancer Genetics. 2019.
Authors Russell J. Diefenbach, Jenny H. Lee, Richard F. Kefford, Helen Rizos,

Maxi kit (Dx55800)

Title Evaluation of commercial kits for purification of circulating free DNA
Journal Cancer Genetics. 2019.
Authors Russell J. Diefenbach, Jenny H. Lee, Richard F. Kefford, Helen Rizos,

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