For rapid and simple purification of cell-free circulating DNA from plasma/serum samples

  • Isolate all sizes of circulating DNA from plasma and serum samples
  • Isolate viral and bacterial DNA
  • Versatile plasma and serum input volumes (200 µL - 500 µL)
  • Concentrate circulating DNA into a flexible elution volume ranging from (25 µL - 50 µL)
  • Isolate inhibitor-free cell-free circulating DNA
  • Purify high-quality DNA in 15-20 minutes
  • Compatible with Streck Cell-Free DNA BCT® Tubes

ItemsCat. #Size
Plasma/Serum Cell-Free Circulating DNA Purification Mini Kit 55100 50 preps

Plasma/Serum Cell-Free Circulating DNA Purification Mini Kit

This kit provides a fast, reliable and convenient spin column method for the isolation of high quality, high purity and inhibitor-free cell-free circulating DNA (cfc-DNA) from plasma/serum sample volumes ranging from 200 μL up to 500 μL.

The kit is designed to isolate all sizes of cfc-DNA from either fresh or frozen plasma/serum samples and the purified DNA is eluted into a flexible elution volume ranging from 25 µL to 50 µL. The purified plasma/serum cfc-DNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays and NGS.

Background

Plasma/Serum cell-free circulating DNA (cfc-DNA) has the potential to provide biomarkers for certain cancers and disease states as well as fetal DNA in maternal blood. Currently, significant advancements are being made in utilizing cfc-DNA as biomarkers for the early diagnosis, prognosis and monitoring of therapy for several cancer types and autoimmune diseases. Cell-free mitocondrial DNA (cfmtDNA) is also under investigation for its clinical significance. This cfc-DNA is usually present as short fragments of less than 1000 bp. In addition, cell-free fetal DNA has been widely used as a non-invasive method for prenatal diagnosis including early identification of fetal sex, genetic studies for families at high risk for inherited genetic disorders, screening for Rhesus factor, screening for aneuploidy and identification of preeclampsia.

Kit Specifications
Minimum Plasma/Serum Input
200 μL
Maximum Plasma/Serum Input
500 μL
Size of DNA Purified
≥ 50 bp
Elution Volume
25-50 μL
Time to Complete 10 Purifications
15-20 minutes
Average Yields
Variable depending on specimen

*Please check page 7 in the product manual for the Plasma/Serum Average Yields and the Common DNA Quantification Methods.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 years without showing any reduction in performance.

Norgen's Plasma/Serum Cell-Free Circulating DNA Purification Kits contain ready-to-use Proteinase K solution, which is dissolved in a specially prepared storage buffer. The Proteinase K is stable for up to 2.5 years after delivery when stored at room temperature. To prolong the lifetime of Proteinase K, storage at 2–8°C is recommended.


Kit Components
Component Cat. 55100 (50 preps)
Binding Buffer B 2 x 40 mL
Proteinase K 2 x 1 mL
Solution WN 18 mL
Wash Solution A 38 mL
Elution Buffer B 8 mL
Mini Spin Columns 50
Collection Tubes 100
Elution Tubes (1.7 mL) 100
Product Insert 1

Title Advances in Circulating Tumor DNA Analysis
Journal Advances in Clinical Chemistry. 2017.
Authors S. Perakis, M. Auer, J. Belic, E. Heitzer
Title Comprehensive evaluation of methods to isolate, quantify, and characterize circulating cell-free DNA from small volumes of plasma
Journal Analytical and Bioanalytical Chemistry. 2015.
Authors Mauger F, Dulary C, Daviaud C, Deleuze JF, Tost J.