• Isolate all sizes of circulating DNA from plasma and serum samples
  • Isolate viral and bacterial DNA
  • Versatile plasma and serum input volumes (1 mL – 4 mL)
  • Concentrate circulating DNA into a flexible elution volume ranging from (25 µL - 50 µL)
  • Isolate inhibitor-free cell-free circulating DNA
  • Purify high-quality DNA in 90 minutes
  • Compatible with Streck Cell-Free DNA BCT® Tubes

ItemsCat. #Size
Plasma/Serum Cell-Free Circulating DNA Purification Midi Kit 55600 20 preps

Plasma/Serum Cell-Free Circulating DNA Purification Midi Kit

This kit provides a fast, reliable and convenient method to purify and concentrate using a two column method for the isolation of high quality, high purity and inhibitor-free cell-free circulating DNA (cfc-DNA) from fresh or frozen plasma/serum sample volumes ranging from 1 mL - 4 mL.

The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 25 µL to 50 µL. All components for the purification & concentration are provided in one convenient & fast kit for the easy processing of large input volumes of bodily fluids. For a schematic workflow of the protocol click here.

The purified plasma/serum cfc-DNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays and NGS, for various analyses: Fetal DNA screening, genotyping, and other oncology and autoimmune diseases research.

Background

Plasma/Serum cell-free circulating DNA (cfc-DNA) has the potential to provide biomarkers for certain cancers and disease states as well as fetal DNA in maternal blood. Currently, significant advancements are being made in utilizing cfc-DNA as biomarkers for the early diagnosis, prognosis and monitoring of therapy for several cancer types and autoimmune diseases. Cell-free mitocondrial DNA (cfmtDNA) is also under investigation for its clinical significance. This cfc-DNA is usually present as short fragments of less than 1000 bp. In addition, cell-free fetal DNA has been widely used as a non-invasive method for prenatal diagnosis including early identification of fetal sex, genetic studies for families at high risk for inherited genetic disorders, screening for Rhesus factor, screening for aneuploidy and identification of preeclampsia.

Protocol

Kit Components
Minimum Plasma/Serum Input 1 mL
Maximum Plasma/Serum Input 4 mL
Size of DNA Purified ≥ 50 bp
Elution Volume 25-50 μL
Time to Complete 10 Purifications 90 minutes
Average Yields* Variable depending on specimen

*Please check page 7 in the product manual for the Plasma/Serum Average Yields and the Common DNA Quantification Methods.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 years without showing any reduction in performance.

Norgen's Plasma/Serum Cell-Free Circulating DNA Purification Kits contain ready-to-use Proteinase K solution, which is dissolved in a specially prepared storage buffer.

The Proteinase K is stable for up to 2.5 years after delivery when stored at room temperature. To prolong the lifetime of Proteinase K, storage at 2–8°C is recommended.

Title Evaluation of commercial kits for purification of circulating free DNA
Journal Cancer Genetics. 2019.
Authors Russell J. Diefenbach, Jenny H. Lee, Richard F. Kefford, Helen Rizos,