For rapid and simple sequential purification of circulating RNA, exosomal RNA and cell-free circulating DNA from plasma/serum samples

  • Isolate all sizes of circulating and exosomal RNA, including microRNA
  • Isolate all sizes of circulating DNA from plasma and serum samples
  • Isolate viral and bacterial DNA and RNA
  • Versatile plasma and serum input volumes (10 µL - 200 µL)
  • No phenol extractions
  • Bind and elute all RNA irrespective of size or GC content, without bias
  • Concentrate circulating RNA, exosomal RNA and cell-free circulating DNA into a flexible elution volume ranging from 10 µL to 25 µL
  • Isolate inhibitor-free nucleic acids
  • Purify high-quality RNA and DNA in 30 minutes

ItemsCat. #Size
Plasma/Serum RNA/DNA Purification Mini Kit 55200 50 preps

Plasma/Serum RNA/DNA Purification Mini Kit

Norgen's Plasma/Serum RNA/DNA Purification Mini Kit provides a fast, reliable, reproducible and simple procedure for the sequential isolation of circulating RNA, exosomal RNA and Cell-Free Circulating DNA (cfc) from the same Plasma/Serum Sample. It can sequentially isolated RNA and DNA from small plasma/serum input ranging from 10 µL to 200 µL. The kit is designed to isolate all sizes of circulating RNA, including microRNA, all sizes of exosomal RNA as well as all sizes of cfc-DNA from fresh or frozen plasma or serum samples. Norgen's Plasma/Serum RNA Purification Kit provides a clear advantage over other available kits in that it does not require Phenol/Chloroform or any Protease treatments for the isolation of plasma/serum RNA or DNA . RNA and DNA can be eluted into a flexible elution volume ranging from 10 µL to 25 µL. Purified RNA can be used in a number of sensitive downstream applications including reverse transcription qPCR, reverse transcription PCR, NGS, Northern blotting, RNase protection and primer extension, and expression array assays. Purified DNA can be used in a number of sensitive downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis.

Background

Typical yields of free-circulating, exosomal RNA and cfc-DNA vary depending on the input sample, as the amount of RNA and/or DNA present in plasma and serum will depend upon the health status of the individual. Normally, the RNA/DNA yield from plasma or serum is highly variable (range from 1 to 100 ng/mL). Variability is also observed between samples collected from the same donor at different times during the day. This kit is suitable for the isolation of RNA/DNA from serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.

Kit Specifications
Minimum Plasma/Serum Input
10 μL
Maximum Plasma/Serum Input
200 μL
Size of RNA Purified
All sizes including miRNA
and small RNA (<200 nt)
Size of DNA Purified
≥ 50 bp
Elution Volume
10-25 μL
Time to Complete 10 Purifications
15-20 minutes
Average Yields
Variable depending on specimen

Note: Do not exceed the recommended sample input volume of 200 µL.

Storage Conditions and Product Stability

All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 years without showing any reduction in performance. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.


Kit Components
Component Cat. 55200 (50 preps)
Lysis Buffer A 30 mL
Wash Solution A 38 mL
Elution Solution A 6 mL
Elution Buffer B 8 mL
Micro Spin Columns 50
Collection Tube 100
Elution Tubes (1.7 mL) 100
Product Insert 1

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