Figure 2. A representative Bioanalyzer High Sensitivity DNA Chip trace showing the quality/quantity of the cf-DNA purified from 4 mL K2 EDTA plasma A) Norgen’s Plasma/Serum Total cfc-NA Advanced Purification Kit (Automated Isolation), B) Norgen’s Plasma/Serum Total cfc-NA Advanced Purification Kit (Manual Isolation), C) Competitor Q's ccfDNA/RNA Kit and D) Competitor Q's MinElute ccfDNA Midi Kit. All purification methods showed the correct cf-DNA size (~180bp) on the bioanalyzer trace however the amount of the cf-DNA recovered using Norgen’s technology (as can be noticed by the height of the cf-DNA peak) was significantly higher than recovered using the silica-based technology.

Figure 3. Real-Time PCR amplification of miR-21, miR-16 (cfc-RNA targets) and ALU 247, ALU 115 (cfc-DNA targets) from Total cfc-Nucleic acid purified from 4mL K2EDTA plasma using different purification methods.. Norgen’s SiC technology, manual and automated procedure, was superior as compared to the amplification of the same targets amplified from Total Nucleic aicd purified from K2EDTA plasma using the Silica-based technology represented by Competitor Q.