Plasmid DNA Kits

Cat. 13300, 46400, 60300, 63000, 46500, 46600
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Plasmid DNA Kits

Cat. 13300, 46400, 60300, 63000, 46500, 46600

For rapid and convenient plasmid DNA preparations

  • Isolate high quality, high yield plasmid DNA
  • Plasmid DNA is ready for various downstream applications including restriction digestion, bacterial transformation, sequencing and more
  • Available in 4 formats: MiniPrep, MiniPrep (Magnetic Bead System), 96-Well MiniPrep (Magnetic Bead System), and MaxiPrep

For research use only and NOT intended for in vitro diagnostics.

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Plasmid MiniPrep Kit
(Cat. 13300)
50 preps
Plasmid MiniPrep Kit
(Cat. 46400)
250 preps
Plasmid MiniPrep Kit (Magnetic Bead System)
(Cat. 60300)
50 preps
Plasmid MiniPrep 96-Well Kit (Magnetic Bead System)
(Cat. 63000)
2 x 96-well plates
Plasmid DNA MaxiPrep Kit
(Cat. 46500)
4 preps
Plasmid DNA MaxiPrep Kit
(Cat. 46600)
20 preps

Kit Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Kit Size

  • Isolate high quality, high yield plasmid DNA
  • Plasmid DNA is ready for various downstream applications including restriction digestion, bacterial transformation, sequencing and more
  • Available in 4 formats: MiniPrep, MiniPrep (Magnetic Bead System), 96-Well MiniPrep (Magnetic Bead System), and MaxiPrep

Plasmid DNA Kits

These kits are designed for the rapid preparation of plasmid DNA from Escherichia coli.

Plasmid MiniPrep Kit

This kit is designed for the rapid preparation of plasmid DNA from small cultures of Escherichia coli using convenient spin columns. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. This kit is able to purify plasmids up to 13,000 bp in size, and the typical purification yield is up to 20 μg from 1.5 mL of bacterial culture. Purified DNA is of excellent quality for transformation, restriction enzyme digestion, sequencing and more. Also available in a 96-well format.

Plasmid MiniPrep Kit (Magnetic Bead System)

Norgen's Plasmid MiniPrep Kit (Magnetic Bead System) is designed for the rapid preparation of plasmid DNA from small batch cultures of Escherichia coli. Norgen’s magnetic beads bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. The purified plasmids are fully digestible with all restriction enzymes tested, and are completely compatible with real-time PCR and NGS.

Norgen's Plasmid MiniPrep Kit (Magnetic Bead System) is also available in a 96-well format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.

Plasmid MaxiPrep Kit

This kit is designed for the rapid spin column preparation of plasmid DNA from up to 100 mL of Escherichia coli cultures. The kit allows for the isolation of plasmid DNA with final endotoxin levels of 0.1 EU/µg of DNA or less. The kit is able to purify plasmids up to 13,000 bp in size, and typical yields from a 100 mL culture for a high copy number plasmid are between 0.4 and 1.0 mg. The purified DNA is fully digestible with all restriction enzymes tested, and is completely compatible with manual or automated sequencing to achieve 95-100% accuracy.

Kit Specifications

You have selected: Cat. 13300
Kit Specifications
Column Binding Capacity
25 µg
Size of Plasmids Purified
Up to 13,000 bp
Average Yield from 1.5 mL of Culture
Up to 20 µg
Time to Complete 10 Purifications
30 minutes


Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the of shipment. The RNase should be stored at -20°C upon arrival. The Resuspension Solution AZ should be stored at 4oC upon addition of RNase enzyme.

You have selected: Cat. 46400
Kit Specifications
Column Binding Capacity
25 µg
Size of Plasmids Purified
Up to 13,000 bp
Average Yield from 1.5 mL of Culture
Up to 20 µg
Time to Complete 10 Purifications
30 minutes


Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the of shipment. The RNase should be stored at -20°C upon arrival. The Resuspension Solution AZ should be stored at 4°C upon addition of RNase enzyme.

You have selected: Cat. 60300
Kit Specifications
Size of Plasmids Purified
Up to 13,000 bp
Average Yield from 1.5 mL of Culture
Up to 20 μg
Time to Complete 10 Purifications
30 minutes (Cat. 60300)
45 minutes (Cat. 63000)

Storage Conditions and Product Stability
The RNase should be stored at -20°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The Resuspension Solution AZ should be stored at 4°C upon addition of RNase enzyme, and can be stored for up to 6 months after the addition of RNase.

You have selected: Cat. 63000
Kit Specifications
Size of Plasmids Purified
Up to 13,000 bp
Average Yield from 1.5 mL of Culture
Up to 20 μg
Time to Complete 10 Purifications
30 minutes (Cat. 60300)
45 minutes (Cat. 63000)

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year from the date of shipment. The RNase should be stored at -20°C upon arrival. The Resuspension Solution A should be stored at 4°C upon addition of RNase enzyme.

You have selected: Cat. 46500
Kit Specifications
Column Binding Capacity
1.5 mg
Average Yield from 100 mL Culture*
0.4 - 1.0 mg
Final Endotoxin Levels
< 0.1 EU/µg DNA
Time to Complete 4 Purifications
1.5 hours
Size of Plasmids Purified
Up to 13,000 bp

 * Depends on high-copy DNA and low copy DNA


Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The RNase vial should be stored at -20°C upon arrival. Once RNase has been added to the Resuspension Solution AZ the solution should be stored at 4°C. This kit is stable for 1 year after its date of shipment.

You have selected: Cat. 46600
Kit Specifications
Column Binding Capacity
1.5 mg
Average Yield from 100 mL Culture*
0.4 - 1.0 mg
Final Endotoxin Levels
< 0.1 EU/µg DNA
Time to Complete 4 Purifications
1.5 hours
Size of Plasmids Purified
Up to 13,000 bp

 * Depends on high-copy DNA and low copy DNA


Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The RNase vial should be stored at -20°C upon arrival. Once RNase has been added to the Resuspension Solution AZ the solution should be stored at 4°C. This kit is stable for 1 year after its date of shipment.

Supporting Data

Click for expanded view

Component Cat. 13300 (50 preps) Cat. 46400 (250 preps) Cat. 46500 (4 preps) Cat. 46600 (20 preps) Cat. 60300 (50 preps) Cat. 63000 (192 preps)
Resuspension Solution AZ 12 mL 60 mL 20 mL 100 mL 12 mL 2 x 20 mL
Lysis Buffer N 40 mL 80 mL 40 mL 2 x 80 mL 40 mL 2 x 40 mL
Buffer TN 20 mL 130 mL 55 mL 2 x 130 mL 20 mL 1 x 55 mL
1 x 20 mL
Wash Solution E 12 mL 2 x 18 mL - - - -
Elution Buffer K 8 mL 30 mL - - 8 mL 2 x 8 mL
Wash Solution J - - 25 mL 3 x 25 mL - -
Elution Buffer J - - 24 mL 120 mL - -
RNase A 1 vial 1 vial 1 vial 1 vial 1 vial 1 vial
Magnetic Bead Suspension - - - - 1 x 1.1 mL 4 x 1.1 mL
Spin Columns 50 250 - - - -
Collection Tubes 50 250 - - - -
DNA Maxi Spin Columns with Collection Tubes (Clear ring in column) - - 4 20 - -
Maxi Spin Filter Columns with Collection Tubes (Grey ring in column) - - 4 20 - -
96-Well Plate - - - - - 2
Elution Tubes (1.7 mL) 50 250 - - 50 -
Elution Tubes (50 mL) - - 4 20
96-Well Elution Plate - - - - - 2
Product Insert 1 1 1 1

MiniPrep (13300, 46400)

Title Differences in Gene Expression of Pear Selections Showing Leaf Curling or Leaf Reddening Symptoms Due to Pear Decline Phytoplasma
Journal Plants (Basel). 2022.
Authors Mina Kaviani, Paul H Goodwin, David M Hunter
Title Evaluation of aromatic and polycyclic hydrocarbon degrading abilities of selected bacterial isolates
Journal Journal of Microbiology and Biotechnology Research. 2017
Authors Thenmozhi. R, D.Praveenkumar, E.Priya, A. Nagasathy, N.Thajuddin
Title Antimicrobial Resistance Profiles of Listeria monocytogenes and Listeria innocua Isolated from Ready-to-Eat Products of Animal Origin in Spain
Journal Foodborne Pathogens and Disease. 2017
Authors Escolar C, Gómez D, Rota García MD, Conchello P, Herrera A.
Title Plasmid profile of Staphylococcus aureus from orthopaedic patients in Ahmadu Bello University Teaching Hospital Zaria, Nigeria
Journal African Journal of Microbiology Research. 2015.
Authors Obajuluwa, A. F., Olayinka, B. O., Adeshina, G. O., Onaolapo, J. A. and Afolabi-Balogun, N. B.

MaxiPrep (46500, 46600)

Title Role of Endonuclease G in Exogenous DNA Stability in HeLa Cells
Journal International Journal of Biotechnology and Bioengineering. 2016.
Authors Vanja Misic, Mohamed El-Mogy, Yousef Haj-Ahmad

Plasmid DNA Kits

These kits are designed for the rapid preparation of plasmid DNA from Escherichia coli.

Plasmid MiniPrep Kit

This kit is designed for the rapid preparation of plasmid DNA from small cultures of Escherichia coli using convenient spin columns. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. This kit is able to purify plasmids up to 13,000 bp in size, and the typical purification yield is up to 20 μg from 1.5 mL of bacterial culture. Purified DNA is of excellent quality for transformation, restriction enzyme digestion, sequencing and more. Also available in a 96-well format.

Plasmid MiniPrep Kit (Magnetic Bead System)

Norgen's Plasmid MiniPrep Kit (Magnetic Bead System) is designed for the rapid preparation of plasmid DNA from small batch cultures of Escherichia coli. Norgen’s magnetic beads bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. The purified plasmids are fully digestible with all restriction enzymes tested, and are completely compatible with real-time PCR and NGS.

Norgen's Plasmid MiniPrep Kit (Magnetic Bead System) is also available in a 96-well format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.

Plasmid MaxiPrep Kit

This kit is designed for the rapid spin column preparation of plasmid DNA from up to 100 mL of Escherichia coli cultures. The kit allows for the isolation of plasmid DNA with final endotoxin levels of 0.1 EU/µg of DNA or less. The kit is able to purify plasmids up to 13,000 bp in size, and typical yields from a 100 mL culture for a high copy number plasmid are between 0.4 and 1.0 mg. The purified DNA is fully digestible with all restriction enzymes tested, and is completely compatible with manual or automated sequencing to achieve 95-100% accuracy.

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 13300
Kit Specifications
Column Binding Capacity
25 µg
Size of Plasmids Purified
Up to 13,000 bp
Average Yield from 1.5 mL of Culture
Up to 20 µg
Time to Complete 10 Purifications
30 minutes


Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the of shipment. The RNase should be stored at -20°C upon arrival. The Resuspension Solution AZ should be stored at 4oC upon addition of RNase enzyme.

You have selected: Cat. 46400
Kit Specifications
Column Binding Capacity
25 µg
Size of Plasmids Purified
Up to 13,000 bp
Average Yield from 1.5 mL of Culture
Up to 20 µg
Time to Complete 10 Purifications
30 minutes


Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the of shipment. The RNase should be stored at -20°C upon arrival. The Resuspension Solution AZ should be stored at 4°C upon addition of RNase enzyme.

You have selected: Cat. 60300
Kit Specifications
Size of Plasmids Purified
Up to 13,000 bp
Average Yield from 1.5 mL of Culture
Up to 20 μg
Time to Complete 10 Purifications
30 minutes (Cat. 60300)
45 minutes (Cat. 63000)

Storage Conditions and Product Stability
The RNase should be stored at -20°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The Resuspension Solution AZ should be stored at 4°C upon addition of RNase enzyme, and can be stored for up to 6 months after the addition of RNase.

You have selected: Cat. 63000
Kit Specifications
Size of Plasmids Purified
Up to 13,000 bp
Average Yield from 1.5 mL of Culture
Up to 20 μg
Time to Complete 10 Purifications
30 minutes (Cat. 60300)
45 minutes (Cat. 63000)

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year from the date of shipment. The RNase should be stored at -20°C upon arrival. The Resuspension Solution A should be stored at 4°C upon addition of RNase enzyme.

You have selected: Cat. 46500
Kit Specifications
Column Binding Capacity
1.5 mg
Average Yield from 100 mL Culture*
0.4 - 1.0 mg
Final Endotoxin Levels
< 0.1 EU/µg DNA
Time to Complete 4 Purifications
1.5 hours
Size of Plasmids Purified
Up to 13,000 bp

 * Depends on high-copy DNA and low copy DNA


Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The RNase vial should be stored at -20°C upon arrival. Once RNase has been added to the Resuspension Solution AZ the solution should be stored at 4°C. This kit is stable for 1 year after its date of shipment.

You have selected: Cat. 46600
Kit Specifications
Column Binding Capacity
1.5 mg
Average Yield from 100 mL Culture*
0.4 - 1.0 mg
Final Endotoxin Levels
< 0.1 EU/µg DNA
Time to Complete 4 Purifications
1.5 hours
Size of Plasmids Purified
Up to 13,000 bp

 * Depends on high-copy DNA and low copy DNA


Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The RNase vial should be stored at -20°C upon arrival. Once RNase has been added to the Resuspension Solution AZ the solution should be stored at 4°C. This kit is stable for 1 year after its date of shipment.

Component Cat. 13300 (50 preps) Cat. 46400 (250 preps) Cat. 46500 (4 preps) Cat. 46600 (20 preps) Cat. 60300 (50 preps) Cat. 63000 (192 preps)
Resuspension Solution AZ 12 mL 60 mL 20 mL 100 mL 12 mL 2 x 20 mL
Lysis Buffer N 40 mL 80 mL 40 mL 2 x 80 mL 40 mL 2 x 40 mL
Buffer TN 20 mL 130 mL 55 mL 2 x 130 mL 20 mL 1 x 55 mL
1 x 20 mL
Wash Solution E 12 mL 2 x 18 mL - - - -
Elution Buffer K 8 mL 30 mL - - 8 mL 2 x 8 mL
Wash Solution J - - 25 mL 3 x 25 mL - -
Elution Buffer J - - 24 mL 120 mL - -
RNase A 1 vial 1 vial 1 vial 1 vial 1 vial 1 vial
Magnetic Bead Suspension - - - - 1 x 1.1 mL 4 x 1.1 mL
Spin Columns 50 250 - - - -
Collection Tubes 50 250 - - - -
DNA Maxi Spin Columns with Collection Tubes (Clear ring in column) - - 4 20 - -
Maxi Spin Filter Columns with Collection Tubes (Grey ring in column) - - 4 20 - -
96-Well Plate - - - - - 2
Elution Tubes (1.7 mL) 50 250 - - 50 -
Elution Tubes (50 mL) - - 4 20
96-Well Elution Plate - - - - - 2
Product Insert 1 1 1 1

MiniPrep (13300, 46400)

Title Differences in Gene Expression of Pear Selections Showing Leaf Curling or Leaf Reddening Symptoms Due to Pear Decline Phytoplasma
Journal Plants (Basel). 2022.
Authors Mina Kaviani, Paul H Goodwin, David M Hunter
Title Evaluation of aromatic and polycyclic hydrocarbon degrading abilities of selected bacterial isolates
Journal Journal of Microbiology and Biotechnology Research. 2017
Authors Thenmozhi. R, D.Praveenkumar, E.Priya, A. Nagasathy, N.Thajuddin
Title Antimicrobial Resistance Profiles of Listeria monocytogenes and Listeria innocua Isolated from Ready-to-Eat Products of Animal Origin in Spain
Journal Foodborne Pathogens and Disease. 2017
Authors Escolar C, Gómez D, Rota García MD, Conchello P, Herrera A.
Title Plasmid profile of Staphylococcus aureus from orthopaedic patients in Ahmadu Bello University Teaching Hospital Zaria, Nigeria
Journal African Journal of Microbiology Research. 2015.
Authors Obajuluwa, A. F., Olayinka, B. O., Adeshina, G. O., Onaolapo, J. A. and Afolabi-Balogun, N. B.

MaxiPrep (46500, 46600)

Title Role of Endonuclease G in Exogenous DNA Stability in HeLa Cells
Journal International Journal of Biotechnology and Bioengineering. 2016.
Authors Vanja Misic, Mohamed El-Mogy, Yousef Haj-Ahmad

 


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