PCR Sizer 100 bp DNA Ladder
Ideal for PCR product size confirmation
For research use only and NOT intended for in vitro diagnostics.
Powered by Bioz PCR Sizer 100 bp DNA Ladder
Ideal for PCR product size confirmation
$75.00
SKU
11400
Overview
- Ready-to-use
- Quantitative
- Highly Stable
- Precise
- Ten discrete fragments from 100 bp to 1000 bp
- Higher intensity reference band at 500 bp
The Norgen PCRSizer 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our PCRSizer contains ten discrete fragments ranging from 100 bp to 1000 bp in 100 bp increments with a higher intensity reference band at 500 bp. This Ladder is ideal for PCR product size confirmation.
Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
Details
Supporting Data
PCR Sizer 100 bp DNA Ladder (Cat# 11400) - 100 loads
Ladder Properties:
• Ten discrete bands, ranging from 100 bp to 1,000 bp
• Higher intensity band at 500 bp for easy reference
• Ten discrete bands, ranging from 100 bp to 1,000 bp
• Higher intensity band at 500 bp for easy reference
|
Fragment
|
Size (bp)
|
Mass (ng)
|
|
1
|
1000
|
78
|
|
2
|
900
|
70
|
|
3
|
800
|
62
|
|
4
|
700
|
55
|
|
5
|
600
|
47
|
|
6
|
500
|
78
|
|
7
|
400
|
31
|
|
8
|
300
|
24
|
|
9
|
200
|
31
|
|
10
|
100
|
24
|
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
Documentation
Citations
| Title | Virulence Genes of Pathogenic Escherichia coli in Wild Red Foxes (Vulpes vulpes) |
| Journal | Animals . 2017. |
| Authors | Fabrizio Bertelloni, Giulia Cagnoli, Fabrizio Biagini, Alessandro Poli, Carlo Bibbiani and Valentina Virginia Ebani |
| Title | Distribution Profile of Extended Spectrum Beta Lactamase (ESBL) Producing Escherichia coli Isolates from Asa River (Nigeria) |
| Journal | Journal of Applied Sciences and Environmental Management. 2017. |
| Authors | Matthew Kolawole Olatunji, Albert Olayemi, Fausat Jimoh |
| Title | Standardization of a molecular method for epidemiologic identification of Leishmania strains |
| Journal | Genet. Mol. Res. 2016. |
| Authors | D.A. Oliveira, E.V. Menezes, V.A. Royo, A.R.E.O. Xavier, R.F. Rocha, A.F.M. Júnior, E.S. Dias, A.C.V.M.R. Lima, E.M. Michalsky |
| Title | Occurrence and molecular analysis of quarantine virus in lily cultivation areas in Brazil |
| Journal | Pesq. agropec. bras., Brasília. 2016. |
| Authors | Rivas, E. B., de Almeida Bôdi, E. C., Harakava, R., Gregori, F., & Gonçalves, M. C |
| Title | Food control and a citizen science approach for improving teaching of Genetics in universities |
| Journal | IUBMB Journals. 2016. |
| Authors | Borrell YJ, Muñoz-Colmenero AM, Dopico E, Miralles L, Garcia-Vazquez E |
| Title | Establishment of a bank of positive controls for diagnosis of quarantine viruses and viroids in Brazil through PCR and RT-PCR |
| Journal | Crop Protection. 2016. |
| Authors | Priscilla De Sousa Geraldino Duarte, Antonia Dos Reis Figueira, Suellen Barbara Ferreira Galvino-Costa, Anderson De Jesus Sotero, Daniele Costa Pompeu, João Ronaldo Clemente Fernandes, André Luiz De Andrade Carvalho |
| Title | Is Malassezia nana the main species in horses’ ear canal microbiome? |
| Journal | Brazilian Journal of Microbiology. 2016. |
| Authors | Aldrovandi, A. L., Osugui, L., & Coutinho, S. D. A |
| Title | Evidence of helminth infection in guanche mummies: Integrating paleoparasitological and paleogenetic investigations |
| Journal | Journal of Parasitology. 2015. |
| Authors | Iniguez AM, Gijà N-Botella H, Jaeger L, Del Arco-Aguilar MA, Del Arco-Aguilar MA, Araujo A, Rodrà Guez-Maffiotte C, Martà N-Oval M. |
| Title | Analysis of Immunological, Viral, Genetic, and Environmental Factors That Might Be Associated with Decreased Susceptibility to HIV Infection in Serodiscordant Couples in Florianópolis, Southern Brazil |
| Journal | AIDS Research and Human Retroviruses. 2015. |
| Authors | Santos ÍM, da Rosa EA, Gräf T, Ferreira LG, Petry A, Cavalheiro F, Reiche EM, Zanetti CR, Pinto AR |
| Title | Escherichia coli from Crohn’s disease patient displays virulence features of enteroinvasive (EIEC), enterohemorragic (EHEC), and enteroaggregative (EAEC) pathotypes. |
| Journal | Gut Pathogens. 2015. |
| Authors | Ana Carolina da Silva Santos, Fernando Gomes Romeiro, Ligia Yukie Sassaki and Josias Rodrigues |
| Title | Pathogenesis of acute and chronic diseases caused by cyprinid herpesvirus-3. |
| Journal | Journal of Fish Diseases. 2014. |
| Authors | S Miwa, I Kiryu, K Yuasa, T Ito and T Kaneko. |
| Title | Geography has more influence than language on maternal genetic structure of various northeastern Thai ethnicities. |
| Journal | Journal of Human Genetics. 2014. |
| Authors | Wibhu Kutanan1, Silvia Ghirotto, Giorgio Bertorelle, Suparat Srithawong, Kanokpohn Srithongdaeng,Nattapon Pontham and Daoroong Kangwanpong |
| Title | Genetic diversity in the germplasm of tropical maize landraces determined using molecular markers. |
| Journal | Genetic and Molecular Research. 2013. |
| Authors | Molin D, Coelho CJ, Mˆximo DS, Ferreira FS, Gardingo JR, Matiello RR. |
| Title | Characterization of denitrifying granular sludge with and without the addition of external carbon source. |
| Journal | Bioresource Technology. 2012. |
| Authors | Lew B, Stief P, Beliavski M, Ashkenazi A, Svitlica O, Khan A, Tarre S, de Beer D, Green M. |
| Title | Sequencing and expression analysis of hepcidin mRNA in donkey (Equus asinus) liver. |
| Journal | Pesq. Vet. Bras. 2012. |
| Authors | Oliveira-Filho J, Marques J, Cunha P, Medeiros G, Riet-Correa F, Machado V, Borges A. |
| Title | Unraveling a mechanism of honey antibacterial action: Polyphenol/H2O2-induced oxidative effect on bacterial cell growth and on DNA degradation. |
| Journal | Food Chemistry. 2012. |
| Authors | Brudzynski K, Abubaker K, Miotto D. |
| Title | Re-examining the role of hydrogen peroxide in bacteriostatic and bactericidal activities of honey. |
| Journal | Frontiers in Microbiology. 2011. |
| Authors | Brudzynski K, Abubaker K, Laurent M, Castle A. |
| Title | The influence of initial feeding on muscle development and growth in pacu Piaractus mesopotamicus larvae. |
| Journal | Aquaculture. 2011. |
| Authors | Natalia de Jesus Leitão, Maeli Dal Pai-Silva, Fernanda Losi Alves de Almeida, Maria Célia Portella. |
| Title | The beta4-beta8 groove is an ATP-interactive site in the alpha crystallin core domain of the small heat shock protein, human alphaB crystallin. |
| Journal | Journal of Molecular Biology. 2006. |
| Authors | Ghosh JG, Houck SA, Doneanu CE, Clark JI. |