For rapid and convenient plasmid DNA preparations

  • High yield of plasmid DNA
  • Purify plasmids up to 13 kb
  • Plasmid DNA is ready for restriction digestion, bacterial transformation, sequencing and more
  • Rapid spin-column format and 96-well format available

ItemsCat. #Size
Plasmid MiniPrep Kit 13300 50 preps
Plasmid MiniPrep Kit 46400 250 preps

Plasmid MiniPrep Kit

This kit is designed for the rapid preparation of plasmid DNA from small cultures of Escherichia coli using convenient spin columns. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. This kit is able to purify plasmids up to 13,000 bp in size, and the typical purification yield is up to 20 μg from 1.5 mL of bacterial culture. Purified DNA is of excellent quality for transformation, restriction enzyme digestion, sequencing and more. Also available in a 96-well format.

Kit Specifications
Column Binding Capacity
25 µg
Size of Plasmids Purified
Up to 13,000 bp
Average Yield from 1.5 mL of Culture
Up to 20 µg
Time to Complete 10 Purifications
30 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers. The RNAse should be stored at -20°C upon arrival. The Resuspension Buffer should be stored at 4°C upon addition of RNAse enzyme.

Title Evaluation of aromatic and polycyclic hydrocarbon degrading abilities of selected bacterial isolates
Journal Journal of Microbiology and Biotechnology Research. 2017
Authors Thenmozhi. R, D.Praveenkumar, E.Priya, A. Nagasathy, N.Thajuddin
Title Antimicrobial Resistance Profiles of Listeria monocytogenes and Listeria innocua Isolated from Ready-to-Eat Products of Animal Origin in Spain
Journal Foodborne Pathogens and Disease. 2017
Authors Escolar C, Gómez D, Rota García MD, Conchello P, Herrera A.
Title Plasmid profile of Staphylococcus aureus from orthopaedic patients in Ahmadu Bello University Teaching Hospital Zaria, Nigeria
Journal African Journal of Microbiology Research. 2015.
Authors Obajuluwa, A. F., Olayinka, B. O., Adeshina, G. O., Onaolapo, J. A. and Afolabi-Balogun, N. B.