Pneumocystis jirovecii TaqMan PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Pneumocystis jirovecii TaqMan PCR Detection Kits Price USD$699.00 SKU TM42850 Format PCR Probe/Primer and Control Set Size 100 Preps Quantity Overview Details Documentation Citations Overview Detection kits for Pneumocystis jirovecii Available in TaqMan format for analysis Pneumocystis jirovecii is a yeast-like fungus that causes Pneumocystis jirovecii Pneumonia (originally known as Pneumocystis carinii Pneumonia or PCP). PCP is the most common opportunistic infection in patients with HIV/AIDS. Species of Pneumocystis are commonly found in the lungs of healthy individuals. In fact, most children are believed to be exposed by age 3 or 4 years. Studies have suggested that P. jirovecii is communicable, possibly via airborne transmission. Disease usually develops in patients whose cellular immunity and humoral immunity are defective. Even with the widespread use of highly active antiretroviral therapy (HAART), there is still a high prevalence of PCP in HIV patients. TaqMan PCR Kit Click to expand options Pneumocystis jirovecii TaqMan PCR Kit, 100 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents Pneumocystis jirovecii TaqMan PCR Probe/Primer Set and Controls, 100 reactions Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest Nuclease-free water Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix For research use only and NOT intended for in vitro diagnostics. Details Supporting Data Previous TaqMan PCR Kit - Figure 1. Example of TaqMan One-step RT-PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 2. Example of TaqMan One-step RT-PCR Negative result. No target RNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR. TaqMan PCR Kit - Figure 3. Example of TaqMan one-step RT-PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for RNA purification. Next Click for expanded view Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance. The Pneumocystis jirovecii TaqMan PCR Kit is shipped on dry ice. The components of the kit should be frozen upon arrival. If one or more of the components is not frozen when the kit is received, or if any of the components have been compromised during shipment, please contact Norgen Biotek for assistance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. Component Cat. TM42850 (100 preps) Cat. TM42810 (100 preps) MDx TaqMan 2X PCR Master Mix 2 x 700 μL - P. jiroveci Primer & Probe Mix 280 μL 280 μL P. jiroveci Positive Control 150 μL 150 μL Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL Product Insert 1 1 Documentation PROTOCOLS (TM42850) Pneumocystis jirovecii TaqMan PCR Kit - Protocol (100 rxns) (TM42810) Pneumocystis jirovecii TaqMan Probe/Primer and Control Set - Protocol (100 rxns) Citations Powered by Bioz See more details on Bioz Related Products Blood DNA Isolation Mini Kit Plasma/Serum Cell-Free Circulating DNA Purification Mini Kit
Pneumocystis jirovecii TaqMan PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Pneumocystis jirovecii TaqMan PCR Detection Kits Price USD$699.00 SKU TM42850 Format PCR Probe/Primer and Control Set Size 100 Preps Quantity