RNA/DNA Purification Kits

Cat. 48700, 50300

RNA/DNA Purification Kits

Cat.

For sequential isolation of total RNA and genomic DNA from the same sample

For research use only and NOT intended for in vitro diagnostics.

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RNA/DNA Purification Kit
(Cat. 48700) 50 preps

RNA/DNA Purification Micro Kit
(Cat. 50300) 50 preps

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Format: Spin Column
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Size:50 preps

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Overview

RNA/DNA Purification Kits

Sequentially isolate and purify total RNA and DNA from a single sample
Two column system: one for DNA and one for RNA
The RNA column is for the purification of total RNA including microRNA
No need to split the lysate, or to use phenol or precipitation methods
Rapid and efficient spin column procedure - it takes only 30 minutes
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

These kits provide a rapid method for the isolation and purification of total RNA and DNA sequentially from a single sample of cultured animal cells and tissues, blood, bacteria, yeast, or fungi. The lysate is passed over two columns: 1) a DNA column and 2) an RNA column. Total RNA of all sizes is purified, including microRNA. Both DNA and RNA are of the highest purity and yield.

These kits are ideal for researchers who are interested in studying the genome and transcriptome of a single sample, such as for studies of microRNA profiling, gene expression including gene silencing experiments or mRNA knockdowns, studies involving biomarker discovery, and for characterization of cultured cell lines. Norgen’s RNA/DNA Purification Kits are especially useful for researchers who are isolating macromolecules from precious, difficult to obtain or small samples such as biopsy materials or single foci from cell cultures, as they eliminate the need to fractionate the sample. Furthermore, analysis will be more reliable since the RNA and DNA are derived from the same sample, thereby eliminating inconsistent results. The purified macromolecules are of the highest purity and can be used in a number of different downstream applications

RNA/DNA Purification Kit (Spin Column)

Maximum column binding capacity of 50 μg for RNA and 20 μg for DNA.

RNA/DNA Purification Micro Kit (Micro)

The purified RNA and DNA fractions can be eluted in as little as 20 μL. Ideal for cell number inputs of 500,000 and as little as 5 cells. Maximum column binding capacity of 35 μg for RNA and 10 μg for DNA.

Details

Supporting Data

Figure 1. Recovery of True Total RNA including microRNA from Hamster Heart. Panel A is a 1X MOPS 1% agarose gel showing the RNA that was isolated from 2 different samples of 10 mg hamster heart using Norgen's RNA/DNA Purification Kit. Norgen's RNA/DNA Purification Kit isolated large RNA (represented by 28S and 18S rRNA) with high integrity. Moreover, it provided the added benefit of recovering small RNA without any additional protocol. Panel B is a result from a bioanalyzer resolution of the eluted RNA. Similar to the agarose gel, Norgen's RNA/DNA Purification Kit showed the added benefit of recovering small RNA. The effectivenessofsmall RNA recovery was also demonstrated by gene-specific RT-qPCR. One microgram of RNA was used in RT-qPCR reactions for human S15 (for Large RNA) and miR-21 (for microRNA) genes. The RNA isolated by Norgens RNA/DNA Purification Kit showed detection of both small RNA (Panel C) and the large RNA (Panel D).

Figure 2. Recovery of Intact, High Quality Genomic DNA from Hamster Heart. Panel A is a 1% agarose gel showing the gDNA isolated from the same hamster heart samples above using Norgen's RNA/DNA Purification Kit. Lane M is Norgen's HighRanger 1 kb DNA Ladder and the sample lanes contain 10 µL of each of the 100 µL elutions. The gel showed high quality, and intact genomic DNA. Panel B is the result of qPCR amplification of 25 ng of eluted genomic DNA using 5S rRNA-specific primers. Genomic DNA isolated using Norgen's RNA/DNA Purification Kit is of high quality with effective qPCR amplification.

Figure 3. Recovery of True Total RNA including microRNA from Hamster Liver. Panel A is a 1X MOPS 1% agarose gel showing 3 µL of 20 µL eluted RNA that was isolated from 2 different samples of 5 mg hamster liver using Norgen's RNA/DNA Purification Micro Kit. Norgens RNA/DNA Purification Micro Kit isolated large RNA (represented by 28S and 18S rRNA) with high integrity. Moreover, it provided the added benefit of recovering small RNA without any additional protocol. Panel B is a result from a bioanalyzer resolution of the eluted RNA. Similar to the agarose gel, Norgen's RNA/DNA Purification Micro Kit showed the added benefit of recovering small RNA while isolating very high quality RNA. The effectiveness in small RNA recovery was also demonstrated by gene-specific RT-qPCR. One microgram of RNA was used in RT-qPCR reactions for beta-Actin (for Large RNA) and miR-21 (for microRNA) genes. The RNA isolated by Norgens RNA/DNA Purification Micro Kit showed detection of both small RNA (Panel C) and the large RNA (Panel D).

Figure 4. Recovery of Intact, High Quality Genomic DNA from Hamster Liver. Panel A is a 1% agarose gel showing the gDNA isolated from the same hamster liver samples using Norgens RNA/DNA Purification Micro Kit. Lane M is Norgen's HighRanger 1 kb DNA Ladder and the sample lanes contain 3 µL of each of the 20 µL elutions. The gel showed high quality, and intact genomic DNA. Panel B is the result of qPCR amplification of 25 ng of eluted genomic DNA using 5S rRNA-specific primers. Genomic DNA isolated using Norgens RNA/DNA Purification Micro Kit is of high quality with effective qPCR amplification.

Kit Specifications

Cat.

Kit Specifications
Maximum Column Binding Capacity	50 μg for RNA 20 μg for DNA
Maximum Column Loading Volume	650 μL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Maximum Amount of Starting Material: Animal Cells Animal Tissues Blood Bacteria Yeast Fungi Plant Tissues	5 x 10⁶ cells 25 mg (for most tissues)** 200 μL 1 x 10⁹ cells 1 x 10⁸ cells 50 mg 50 mg
Time to Complete 10 Purifications	30 minutes
Average Yield*: HEK 293 Cells (1 x 10⁶ cells) HEK 293 Cells (1 x 10⁶ cells) Liver (15 mg) Liver (15 mg)	10-15 μg RNA 2-4 μg DNA 30-35 μg RNA 4-6 μg DNA

*Average Yield will vary depending upon a number of factors including species, growth conditions used, and development stage.

**Tissue inputs of up to 40 mg may be used, however for inputs greater than the recommended 25 mg, cross-contamination of the RNA and DNA fractions is possible.

Storage Conditions and Product Stability
Store Proteinase K at -20°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Kit Specifications
Maximum Column Binding Capacity	35 μg for RNA 10 μg for DNA
Maximum Column Loading Volume	650 μL
Elution Volume	20-50 μL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Time to Complete 10 Purifications	30 minutes
Maximum Amount of Starting Material: Animal Cells Animal Tissues Blood	5 x 10⁵ cells 5 mg (for selected tissues) 50 µL
Average Yield* Liver (5 mg) Liver (5 mg)	10-12 μg RNA 1-2 μg DNA

*Average yield will vary depending upon a number of factors including species, growth conditions used, and developmental stage.

Improve Your Workflow with Best-in-Class Total RNA Purification Technology | Learn More

RNA/DNA Purification Kits (Cat. 48700, 50300)

RNA/DNA Purification Kits

RNA/DNA Purification Kits

Format: Spin Column
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Size:50 preps

Request a Product Sample