RNA/DNA Purification Kits For sequential isolation of total RNA and genomic DNA from the same sample For research use only and NOT intended for in vitro diagnostics. Powered by Bioz RNA/DNA Purification Kits For sequential isolation of total RNA and genomic DNA from the same sample Price USD$675.00 SKU 48700 Format Spin Column Micro Size 50 Preps Quantity Request sample Overview Details Documentation Citations Overview Sequentially isolate and purify total RNA and DNA from a single sample Two column system: one for DNA and one for RNA The RNA column is for the purification of total RNA including microRNA No need to split the lysate, or to use phenol or precipitation methods Rapid and efficient spin column procedure - it takes only 30 minutes Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix These kits provide a rapid method for the isolation and purification of total RNA and DNA sequentially from a single sample of cultured animal cells and tissues, blood, bacteria, yeast, or fungi. The lysate is passed over two columns: 1) a DNA column and 2) an RNA column. Total RNA of all sizes is purified, including microRNA. Both DNA and RNA are of the highest purity and yield. These kits are ideal for researchers who are interested in studying the genome and transcriptome of a single sample, such as for studies of microRNA profiling, gene expression including gene silencing experiments or mRNA knockdowns, studies involving biomarker discovery, and for characterization of cultured cell lines. Norgen’s RNA/DNA Purification Kits are especially useful for researchers who are isolating macromolecules from precious, difficult to obtain or small samples such as biopsy materials or single foci from cell cultures, as they eliminate the need to fractionate the sample. Furthermore, analysis will be more reliable since the RNA and DNA are derived from the same sample, thereby eliminating inconsistent results. The purified macromolecules are of the highest purity and can be used in a number of different downstream applications RNA/DNA Purification Kit (Spin Column) Maximum column binding capacity of 50 μg for RNA and 20 μg for DNA. RNA/DNA Purification Micro Kit (Micro) The purified RNA and DNA fractions can be eluted in as little as 20 μL. Ideal for cell number inputs of 500,000 and as little as 5 cells. Maximum column binding capacity of 35 μg for RNA and 10 μg for DNA. Details Supporting Data Previous Figure 1. Recovery of True Total RNA including microRNA from Hamster Heart. Panel A is a 1X MOPS 1% agarose gel showing the RNA that was isolated from 2 different samples of 10 mg hamster heart using Norgen's RNA/DNA Purification Kit. Norgen's RNA/DNA Purification Kit isolated large RNA (represented by 28S and 18S rRNA) with high integrity. Moreover, it provided the added benefit of recovering small RNA without any additional protocol. Panel B is a result from a bioanalyzer resolution of the eluted RNA. Similar to the agarose gel, Norgen's RNA/DNA Purification Kit showed the added benefit of recovering small RNA. The effectiveness of small RNA recovery was also demonstrated by gene-specific RT-qPCR. One microgram of RNA was used in RT-qPCR reactions for human S15 (for Large RNA) and miR-21 (for microRNA) genes. The RNA isolated by Norgen’s RNA/DNA Purification Kit showed detection of both small RNA (Panel C) and the large RNA (Panel D). Figure 2. Recovery of Intact, High Quality Genomic DNA from Hamster Heart. Panel A is a 1% agarose gel showing the gDNA isolated from the same hamster heart samples above using Norgen's RNA/DNA Purification Kit. Lane M is Norgen's HighRanger 1 kb DNA Ladder and the sample lanes contain 10 µL of each of the 100 µL elutions. The gel showed high quality, and intact genomic DNA. Panel B is the result of qPCR amplification of 25 ng of eluted genomic DNA using 5S rRNA-specific primers. Genomic DNA isolated using Norgen's RNA/DNA Purification Kit is of high quality with effective qPCR amplification. Figure 3. Recovery of True Total RNA including microRNA from Hamster Liver. Panel A is a 1X MOPS 1% agarose gel showing 3 µL of 20 µL eluted RNA that was isolated from 2 different samples of 5 mg hamster liver using Norgen's RNA/DNA Purification Micro Kit. Norgen’s RNA/DNA Purification Micro Kit isolated large RNA (represented by 28S and 18S rRNA) with high integrity. Moreover, it provided the added benefit of recovering small RNA without any additional protocol. Panel B is a result from a bioanalyzer resolution of the eluted RNA. Similar to the agarose gel, Norgen's RNA/DNA Purification Micro Kit showed the added benefit of recovering small RNA while isolating very high quality RNA. The effectiveness in small RNA recovery was also demonstrated by gene-specific RT-qPCR. One microgram of RNA was used in RT-qPCR reactions for beta-Actin (for Large RNA) and miR-21 (for microRNA) genes. The RNA isolated by Norgen’s RNA/DNA Purification Micro Kit showed detection of both small RNA (Panel C) and the large RNA (Panel D). Figure 4. Recovery of Intact, High Quality Genomic DNA from Hamster Liver. Panel A is a 1% agarose gel showing the gDNA isolated from the same hamster liver samples using Norgen’s RNA/DNA Purification Micro Kit. Lane M is Norgen's HighRanger 1 kb DNA Ladder and the sample lanes contain 3 µL of each of the 20 µL elutions. The gel showed high quality, and intact genomic DNA. Panel B is the result of qPCR amplification of 25 ng of eluted genomic DNA using 5S rRNA-specific primers. Genomic DNA isolated using Norgen’s RNA/DNA Purification Micro Kit is of high quality with effective qPCR amplification. Next Click for expanded view Kit Specifications Maximum Column Binding Capacity 50 μg for RNA 20 μg for DNA Maximum Column Loading Volume 650 μL Size of RNA Purified All sizes, including small RNA (< 200 nt) Maximum Amount of Starting Material: Animal Cells Animal Tissues Blood Bacteria Yeast Fungi Plant Tissues 5 x 106 cells 25 mg (for most tissues)** 200 μL 1 x 109 cells 1 x 108 cells 50 mg 50 mg Time to Complete 10 Purifications 30 minutes Average Yield*: HEK 293 Cells (1 x 106 cells) HEK 293 Cells (1 x 106 cells) Liver (15 mg) Liver (15 mg) 10-15 μg RNA 2-4 μg DNA 30-35 μg RNA 4-6 μg DNA *Average Yield will vary depending upon a number of factors including species, growth conditions used, and development stage. **Tissue inputs of up to 40 mg may be used, however for inputs greater than the recommended 25 mg, cross-contamination of the RNA and DNA fractions is possible. Storage Conditions and Product Stability Store Proteinase K at -20°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. Component Cat. 48700 (50 preps) Cat. 50300 (50 preps) Buffer SKP 40 mL 40 mL Wash Solution A 2 x 38 mL1 x 18 mL 2 x 38 mL1 x 18 mL Elution Solution A 6 mL 6 mL Elution Buffer F 15 mL 6 mL RNase-Free Water 40 mL 40 mL Proteinase K 2 x 12 mg 2 x 12 mg gDNA Purification Columns 50 - gDNA Purification Micro Columns - 50 RNA Purification Columns 50 - RNA Purification Micro Columns - 50 Collection Tubes 100 100 Elution Tubes (1.7 mL) 100 100 Product Insert 1 1 Documentation PROTOCOLS (48700) RNA/DNA Purification Kit (Spin Column) - Protocol (50 prep) (50300) RNA/DNA Purification Micro Kit (Micro) - Protocol (50 prep) SUPPLEMENTARY PROTOCOLS Optional Oncolumn Digestion of DNA - Supplementary Protocol SAFETY DATA SHEETS (50300) RNA/DNA Purification Micro Kit - Safety Data Sheet POSTERS FLYERS Flyer RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class Citations Powered by Bioz See more details on Bioz Related Products RNA/DNA/Protein Purification Plus Kit Proteinase K in storage buffer (20mg/mL) RNase-Free DNase I Kit
RNA/DNA Purification Kits For sequential isolation of total RNA and genomic DNA from the same sample For research use only and NOT intended for in vitro diagnostics. Powered by Bioz RNA/DNA Purification Kits For sequential isolation of total RNA and genomic DNA from the same sample Price USD$675.00 SKU 48700 Format Spin Column Micro Size 50 Preps Quantity Request sample