Norgen DNA/RNA/Protein kit and proteomics compatibility. Thanks very much for your replies to my questions- as usual, much appreciated! We extracted some colons with the Norgen kit this week and got a RIN of 10 for the first time. Other RINs were all
for first and second elutions, and yields were far better than we have been getting with Trizol and more consistent. Needless to say, we're pretty happy!
Agriculture and Agrifood Canada
RNA/DNA/Protein Purification Plus Kits
For sequential isolation of total RNA, genomic DNA and total proteins from the same sample
For research use only and NOT intended for in vitro diagnostics.
RNA/DNA/Protein Purification Plus Kit (Cat. 47700)50 Preps
RNA/DNA/Protein Purification Plus Micro Kit (Cat. 51600)50 preps
RNA/DNA/Protein Purification 96-Well Plus Kit (Cat. 51700)1 x 96-well plate
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.
Sequentially purify total RNA (and miRNA), DNA and proteins from a single sample
No sample splitting or need to use phenol or precipitation methods
Purify RNA/DNA/Protein from cultured animal cells, tissues, blood, bacteria, yeast, fungi or plants
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
These kits are ideal for researchers who are interested in studying the genome, proteome and transcriptome of a single sample, such as for studies of microRNA profiling, gene expression including gene silencing experiments or mRNA knockdowns, studies involving biomarker discovery, and for characterization of cultured cell lines. Norgen’s RNA/DNA/Protein Purification Plus Kits are especially useful for researchers who are isolating macromolecules from precious, difficult to obtain or small samples such as biopsy materials or single foci from cell cultures, as it eliminates the need to fractionate the sample. Furthermore, analysis will be more reliable since the RNA, DNA and proteins are derived from the same sample, thereby eliminating inconsistent results. The purified macromolecules are of the highest purity and can be used in a number of different downstream applications.
RNA/DNA/Protein Purification Plus Kit
This kit provides a rapid method for the high throughput isolation and purification of total RNA, DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, or yeast. The kit employs two columns: 1) for gDNA purification and 2) for RNA purification utilizing Norgen's resin columns (superior for the binding of all RNA sizes including miRNA). The proteins are also purified on the second column after RNA elution. The proteins are eluted in buffer and are ready for downstream application without any further clean up required. The proteins can be quantified directly, used in western blots, ELISA or mass spectrometry. This kit provides a rapid spin-column method for the isolation and purification of total RNA, genomic DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi or plants.
RNA/DNA/Protein Purification Plus Micro Kit
This kit provides a rapid spin-column method for the isolation and purification of total RNA, DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, microdissected samples including LCM, stem cells, sorted cells, and CTC. The total RNA, genomic DNA and proteins are all column purified in less than 30 minutes. The RNA and DNA can be eluted in as little as 20 µL while the protein can be eluted in as little as 50 µL. This kit provides the same performance as if the samples were isolated from dedicated kits.
RNA/DNA/Protein Purification 96-Well Plus Kit
The kit employs two plates: 1) for DNA purification and 2) for RNA purification utilizing Norgen's resin (superior for the binding of all RNA sizes including miRNA). Please see the protocol schematic below.
Figure 1. Recovery of True Total RNA including microRNA from HEK-293 Cells. Panel A is a 1X MOPS 1% agarose gel showing the RNA that was isolated from 2 different samples of ~ 800,000 HEK-293 cells using either Norgen's RNA/DNA/Protein Purification Plus Kit or a competitor's multiple-analyte purification kit. Both kits isolated large RNA (represented by 28S and 18S rRNA) with high integrity but Norgen's RNA/DNA/Protein Purification Plus Kit provided the added benefit of recovering small RNA without any additional protocol. Panel B is a result from a bioanalyzer resolution of the eluted RNA. Similar to the agarose gel, Norgen's RNA/DNA/Protein Purification Plus Kit showed the added benefit of recovering small RNA. The difference in small RNA recovery was also demonstrated by gene-specific RT-qPCR. One microgram of RNA was used in RT-qPCR reactions for human S15 (for Large RNA) and miR-21 (for microRNA) genes. The RNA isolated by Norgen's RNA/DNA/Protein Purification Plus Kit showed