Figure 2. Recovery of True Total RNA including microRNA from Hamster Liver. Panel A is a 1X MOPS 1% agarose gel showing the RNA that was isolated from 2 different samples of 15 mg hamster liver using either Norgen's RNA/DNA/Protein Purification Plus Kit or a competitor's multiple-analyte purification kit. Both kits isolated large RNA (represented by 28S and 18S rRNA) with high integrity but Norgen's RNA/DNA/Protein Purification Plus Kit provided the added benefit of recovering small RNA without any additional protocol. Panel B is a result from a bioanalyzer resolution of the eluted RNA. Similar to the agarose gel, Norgen's RNA/DNA/Protein Purification Plus Kit showed the added benefit of recovering small RNA. The difference in small RNA recovery was also demonstrated by gene-specific RT-qPCR. One microgram of RNA was used in RT-qPCR reactions for hamster beta-Actin (for Large RNA) and miR-21 (for microRNA) genes. The RNA isolated by Norgen's RNA/DNA/Protein Purification Plus Kit showed similar Ct value to RNA isolated by the competitor's kit for the large RNA (Panel D). However, Norgen's RNA/DNA/Protein Purification Plus Kit showed superior recovery of small RNA including microRNAs as depicted by the miR-21 RT-qPCR (Panel C).

Figure 3. Recovery of Intact, High Quality Genomic DNA from HEK-293 cells and Hamster Liver. Panel A is a 1% agarose gel showing the gDNA isolated from the same HEK-293 cell or hamster liver samples using Norgen's RNA/DNA/Protein Purification Plus Kit or competitor's multiple-analyte purification kit. Lane M is Norgen's HighRanger 1 kb DNA Ladder and the sample lanes contain 10 µL of each of the 100 µL elutions. The gel showed high quality, and intact genomic DNA, with a better yield using Norgen's RNA/DNA/Protein Purification Plus Kit. Panel B is the result of qPCR amplification of 25 ng of eluted genomic DNA using 5S rRNA-specific primers. Genomic DNA isolated using Norgen's RNA/DNA/Protein Purification Plus Kit is of high quality and performed similar to or better than competitor's product.

Figure 4. High Quality Total Proteins Eluted in Mass Spec-Compatible Buffer. Norgen's RNA/DNA/Protein Purification Plus Kit provides an additional column purification step for effective concentration and clean-up of the isolated proteins. In contrast, most competing multiple analyte isolation products require protein precipitation and the precipitated proteins are required to be resuspended in buffer with high-detergent content (such as SDS-PAGE loading dye) for full recovery. Protein fractions (from hamster liver) isolated by Norgen's RNA/DNA/Protein Purification Plus Kit and a competitor's kit were resolved on a 12% SDS-PAGE protein gel. Panel A showed that when the competitor's precipitated protein fraction was resuspended in a provided SDS-PAGE loading buffer, the protein recovery was similar among the two kits. Panel B showed that when the same precipitated protein fraction from the competitor's kit was resuspended in a Tris-based buffer containing no detergent or denaturant, the protein recovery became drastically reduced. In contrary, Norgen's RNA/DNA/Protein Purification Plus Kit purified proteins by column and the eluted proteins are already in a buffer compatible with most downstream applications including mass spectrophotometry as well as standard protein quantification methods (including Bradford assays).