RSV-A TaqMan Probe/Primer and Control Set (Cat. TM34210)100 rxns
Format: RT-PCR
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Overview
Respiratory Syncytial Virus A (RSV-A) TaqMan RT-PCR Detection Kits
Detection kits for RSV-A
Available in TaqMan format for analysis
Norgen’s Respiratory Syncytial Virus A (RSV-A) TaqMan RT-PCR Detection Kit is designed for the detection of RSV-A specific RNA in a real-time PCR based on the use of TaqMan technology. This kit is designed for research use only and not for use in diagnostic procedures. The detection of RSV-A specific RNA is based on TaqMan one-step RT-PCR providing a simple, reliable and rapid result for the detection of RSV-A infection. Norgen’s TaqMan RT-PCR Kit includes a PCR control to monitor for PCR inhibition, and to validate the quality of the sample and the detection result. The RSV-A TaqMan RT-PCR Kit comprises Master Mix for the target and PCR control detection, Primer & Probe Mix, as well as a positive control and a negative control (nuclease-free water) to confirm the integrity of the kit reagents.
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RSV-A TaqMan RT-PCR Kit, 100 reactions
Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents
RSV-A TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions
Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix
For research use only and NOT intended for in vitro diagnostics.
TaqMan PCR Kit - Figure 1. Example of TaqMan One-step RT-PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR.
TaqMan PCR Kit -Figure 2. Example of TaqMan One-step RT-PCR Negative result. No target RNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR.
TaqManPCR Kit-Figure 3. Example of TaqMan one-step RT-PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for RNA purification.
Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival.