Saliva DNA Collection and Preservation Devices (50) (Cat. RU49000)

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Sample Collection and Preservation Kits
Saliva Sample Preparation Kits

Saliva DNA Collection and Preservation Devices (50)

Cat. RU49000
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What Scientists are Saying About this Product

Murdoch

We have been using Norgen's Saliva DNA Collection and Preservation Devices Cat. RU49000 and the Norgen's Saliva DNA Isolation Kit (Cat. RU45400) for many years for our world wide projects that include populations from different countries with very challenging collection and storage conditions. The kits have helped us even when the samples had to stay at tropical room temperature for long period of time. The kits worked perfectly for us. We also use the kits in our mail-based DNA collections to outreach the distant communities in Western Australia to offer people the opportunity to participate in genomic research. This way the Norgen kits help us to engage underrepresented populations and communities in our genomic studies.


  • Murdoch university

Saliva DNA Collection and Preservation Devices (50)

Cat. RU49000

For simple and non-invasive saliva collection and preservation of DNA in saliva samples at ambient temperature

  • Reliable and cost-effective
  • Non-invasive, user-friendly sample collection
  • Sample collection and preservation in one convenient kit
  • Samples are non-infectious and can be handled and shipped safely
  • Preserved DNA is stable for 2 years at ambient temperature
  • Compatible with most DNA isolation methods
  • High quality DNA is suitable for sensitive downstream applications including PCR, qPCR, sequencing, SNP analysis, microarrays, RFLP and Southern Blot Analysis
  • Shipping accessories can be purchased separately

For research use only and NOT intended for in vitro diagnostics.

CE-IVDR marked diagnostic version available here

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Saliva DNA Collection and Preservation Devices
(Cat. RU49000) 50 Devices

Product Size

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Kit Specifications

You have selected: Cat. RU49000
Kit Specifications
Volume of Saliva Collected
2 mL
Volume of Saliva-Preservative Mix
4 mL
Preservation Temperature
Room Temperature
Preservation time
Over 2 years at room temperature

Shelf Life and Handling

  • When stored at room temperature, unused Norgen Saliva Preservative Tubes are stable through to the collect-before date without any reduction in performance. Please see the device insert or tube label for collect-before date.
  • Once collected, saliva is stable for more than 2 years when kept tightly sealed and stored at room temperature.
  • The Collection Tube, the Collection Funnel and the Device Container of each Individual Saliva DNA Collection and Preservation Device are recyclable.

Supporting Data

Click for expanded view

Component Cat. RU49000 (50 Devices)
Individual Saliva DNA Collection and Preservation Devices 50
Donor Procedure Flowchart 1
Product Insert 1
   
Individual Saliva DNA Collection and Preservation Device Contents  
Saliva Collection Funnel and Collection Tube 1
Collection Tube Cap 1
Preservative Ampoule 1
Donor Instructions 1

Protocols: 
(RU49000) Saliva DNA Collection and Preservation Devices - Protocol (50 devices)
Application Notes: 
High Efficiency Real-Time PCR from Saliva DNA
Inhibitory Effect of Norgen’s Saliva DNA Preservative on the Growth of Bacteria and Yeast
Isolation of High Quality Saliva DNA from Variable Donor Saliva Samples
Long Term Stability of DNA Stored in Norgen’s Saliva DNA Preservative
Sequencing Analysis of Saliva DNA Isolated using Norgen’s Saliva DNA Collection, Preservation and Isolation Kit
A Comparative Study Between Two Saliva DNA Preservation Systems, and Two Column-Based Saliva DNA Purification Methods
Comparison of Norgen’s Saliva DNA Collection, Preservation and Isolation Kit to a Leading Competitor’s Kit
DNA Isolation from Saliva Preserved using Norgen’s Saliva DNA Collection and Preservation Device using Beckman Coulter Agencourt
DNA Isolation from Saliva Preserved with Norgen’s Saliva DNA Collection and Preservation Device using Qiagen’s QIAamp DNA Blood
Efficient Restriction Enzyme Digestion of Saliva DNA isolated using Norgen’s Saliva DNA Collection, Preservation & Isolation Kit
Stability of DNA Stored in Norgen’s Saliva DNA Preservative for 52 Months at Room Temperature
The Use of Saliva Swabs in Norgen’s Saliva DNA Collection, Preservation and Isolation Kit
Linearity of DNA Isolated from Increasing Volumes of Preserved Saliva Using Norgen’s Saliva DNA Isolation Kit
The Effect of Elution Volume on DNA Quantity and Quality Using Norgen’s Saliva DNA Isolation Kit
Comparison of DNA Isolated from Saliva using Norgen’s Preservative and Saliva DNA Isolation Kit Vs Competitor's
Stability of DNA Stored in Norgen’s Saliva DNA Preservative at 55°C
Safety Data Sheets: 
(RU49000) Saliva DNA Collection and Preservation Devices - Safety Data Sheet
Posters: 
In‐Depth Analysis of the Microbial Diversity in Saliva Samples Preserved at Ambient Temperature for over 6 Years - PAG 2016
Rapid and qPCR ready DNA isolation from environmental and human bodily fluid samples using Magnetic bead system
Viability Of Microorganisms in Saliva & Stool Collection and Transportation Preservatives
Product Flyers: 
Saliva DNA Preservation

Title At age 9, the methylome of assisted reproductive technology children that underwent embryo culture in different media is not significantly different on a genome-wide scale
Journal Human Reproduction. 2022.
Authors Rebekka M Koeck, Florence Busato, Jorg Tost, Heleen Zandstra, Sylvie Remy, Sabine Langie, Marij Gielen, Ron van Golde, John C M Dumoulin, Han Brunner, Masoud Zamani Esteki, Aafke P A van Montfoort
Title Exposure to traffic-related air pollution and bacterial diversity in the lower respiratory tract of children
Journal PLOS ONE. 2021
Authors Niemeier-Walsh, C., Ryan, P. H., Meller, J., Ollberding, N. J., Adhikari, A., & Reponen, T.
Title Exploring influences on food choice in a large population sample: the Italian Taste Project
Journal Food Quality and Preference. 2017
Authors E. Monteleone, S. Spinelli, C. Dinnella, I. Endrizzi, M. Laureati, E. Pagliarini, F. Sinesio, F. Gasperi, L. Torri, E. Aprea, L.I. Bailetti, A. Bendini, A. Braghieri, C. Cattaneo, D. Cliceri, N. Condelli, M.C. Cravero, A. Del Caro, R. Di Monaco, S. Drago, S. Favotto, R. Fusi, L. Galassi, T. Gallina Toschi, A. Garavaldi, P. Gasparini, E. Gatti, C. Masi, A. Mazzaglia, E. Moneta, E. Piasentier, M. Piochi, N. Pirastu, S. Predieri, A. Robino, F. Russo, F. Tesini
Title Sugar-Sweetened Beverage Consumption Is Adversely Associated with Childhood Attention Deficit/Hyperactivity Disorder
Journal International Journal of Environmental Research and Public Health. 2016.
Authors Yu CJ, Du JC, Chiou HC, Feng CC, Chung MY, Yang W, Chen YS, Chien LC, Hwang B, Chen ML
Title Detection of Novel Integrons in the Metagenome of Human Saliva
Journal PLOS ONE. 2016.
Authors Tansirichaiya S, Rahman MA, Antepowicz A, Mullany P, Roberts AP
Title Increased risk of attention-deficit/hyperactivity disorder associated with exposure to organophosphate pesticide in Taiwanese children
Journal Andrology. 2016.
Authors Yu CJ, Du JC, Chiou HC, Chung MY, Yang W, Chen YS, Fuh MR, Chien LC, Hwang B, Chen ML
Title Attention Deficit/Hyperactivity Disorder and Urinary Nonylphenol Levels: A Case-Control Study in Taiwanese Children
Journal PLOS ONE. 2016.
Authors Yu CJ, Du JC, Chiou HC, Yang SH, Liao KW, Yang W, Chung MY, Chien LC, Hwang B, Chen ML
Title Functional polymorphisms in the P2X7 receptor gene are associated with stress fracture injury
Journal Purinergic Signalling. 2016.
Authors Varley I, Greeves JP, Sale C, Friedman E, Moran DS, Yanovich R, Wilson PJ, Gartland A, Hughes DC, Stellingwerff T, Ranson C, Fraser WD, Gallagher JA
Title Selective familiarity deficits in otherwise cognitively intact aging individuals with genetic risk for Alzheimer's disease
Journal Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring. 2015.
Authors Dorothee Schoemaker, Judes Poirier, Sophia Escobar, Serge Gauthier, Jens Pruessner
Title The dentin phosphoprotein repeat region and inherited defects of dentin
Journal Molecular Genetics & Genomic Medicine. 2015.
Authors Jie Yang, Kazuhiko Kawasaki, Moses Lee, Bryan M. Reid, Stephanie M. Nunez, Murim Choi, Figen Seymen, Mine Koruyucu, Yelda Kasimoglu, Ninna Estrella-Yuson, Brent P. J. Lin, James P. Simmer and Jan C.-C. Hu
Title Taurodontism, variations in tooth number, and misshapened crowns in Wnt10a null mice and human kindreds.
Journal Molecular Genetics & Genomic Medicine. 2014.
Authors Jie Yang, Shih-Kai Wang, Murim Choi, Bryan M. Reid, Yuanyuan Hu, Yuan-Ling Lee, Curtis R. Herzog, Hera Kim-Berman, Moses Lee, Paul J. Benke, K. C. Kent Lloyd, James P. Simmer and Jan C.-C. Hu.
Title Patterns of Admixture and Population Structure in Native Populations of Northwest North America.
Journal PLOS Genetics. 2014.
Authors Paul Verdu, Trevor J. Pemberton, Romain Laurent, Brian M. Kemp, Angelica Gonzalez-Oliver, Clara Gorodezky, Cris E. Hughes, Milena R. Shattuck,Barbara Petzelt, Joycelynn Mitchell, Harold Harry, Theresa William, Rosita Worl, Jerome S. Cybulski, Noah A. Rosenberg, Ripan S. Malhi.
Title Association of premenstrual/menstrual symptoms with perinatal depression and a polymorphic repeat in the polyglutamine tract of the retinoic acid induced 1 gene
Journal Journal of Affective Disorders. 2014.
Authors Ene-Choo Tan, Hui-San Tan, Tze-Ern Chua, Theresa Lee, Jasmine Ng, Ying-Chia Ch’ng, Chih-Huei Choo, Helen Y Chen
Title RANK/RANKL/OPG pathway: Genetic associations with stress fracture period prevalence in elite athletes.
Journal The Bone Journal. 2014.
Authors Ian Varley, David C. Hughes, Julie P. Greeves, Trent Stellingwerff, Craig Ranson, William D. Fraser, Craig Sale.

Saliva DNA Collection and Preservation Devices (50)

Norgen's Saliva DNA Collection and Preservation Devices are designed for 1) simple and non-invasive saliva collection and 2) preservation of DNA in saliva samples at ambient temperature. Each of the 50 Saliva DNA Collection and Preservation Devices consists of 3 components: (1) Saliva Collection Funnel and Collection Tube, (2) Collection Tube Cap, and (3) Norgen's Saliva DNA Preservative contained within a sealed squeezable ampoule. Saliva samples are collected by spitting inside the Collection Funnel which has been assembled with the Collection Tube. After collecting the required volume of saliva the Collection Funnel is removed and the contents of the Preservative Ampoule are then added and mixed with the collected saliva. The Saliva Collection Tube is subsequently sent to the laboratory for DNA isolation and analysis. DNA can be isolated from the preserved saliva samples using Norgen's Saliva DNA Isolation Kit (Cat# RU45400). Each of Norgen's Collection Tubes is labeled with a unique serial number that can be used for secure and anonymous tracking of the sample. The saliva DNA in preserved samples is stable for more than 2 years at room temperature. This kit is ideal for collecting and preserving DNA samples for epidemiological and population studies.

Saliva DNA Preservative

Norgen's Saliva DNA Preservative is an aqueous storage buffer designed for rapid cellular lysis and subsequent preservation of DNA from fresh specimens. The buffer prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the buffer eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperature. The components of the buffer allow samples to be stored for more than 2 years without any detectable DNA degradation.

DNA Isolation from Preservative

Prior to saliva DNA isolation, vortex the Collection Tube containing preserved saliva for 10 seconds and incubate at 55°C for one hour.  Saliva DNA can now be isolated from the preserved saliva samples using any commercially available method, including Norgen’s Saliva DNA Isolation Kit (Cat# RU45400), Norgen’s Saliva DNA Isolation 96-Well Kit (Cat. RU35200) and Norgen’s Saliva DNA Isolation Kit (Magnetic Bead System) (Cat. RU55400, RU62900).  Also, Norgen’s Saliva DNA Isolation Reagent Kit (up to 4 mL) (Cat. RU35720) allows for DNA isolation from the preserved saliva samples using an alcohol precipitation method. The purified DNA is of the highest quality and can be used in a number of downstream applications including PCR, NGS and microarray analysis.

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. RU49000
Kit Specifications
Volume of Saliva Collected
2 mL
Volume of Saliva-Preservative Mix
4 mL
Preservation Temperature
Room Temperature
Preservation time
Over 2 years at room temperature

Shelf Life and Handling

  • When stored at room temperature, unused Norgen Saliva Preservative Tubes are stable through to the collect-before date without any reduction in performance. Please see the device insert or tube label for collect-before date.
  • Once collected, saliva is stable for more than 2 years when kept tightly sealed and stored at room temperature.
  • The Collection Tube, the Collection Funnel and the Device Container of each Individual Saliva DNA Collection and Preservation Device are recyclable.
Component Cat. RU49000 (50 Devices)
Individual Saliva DNA Collection and Preservation Devices 50
Donor Procedure Flowchart 1
Product Insert 1
   
Individual Saliva DNA Collection and Preservation Device Contents  
Saliva Collection Funnel and Collection Tube 1
Collection Tube Cap 1
Preservative Ampoule 1
Donor Instructions 1
Protocols: 
(RU49000) Saliva DNA Collection and Preservation Devices - Protocol (50 devices)
Application Notes: 
High Efficiency Real-Time PCR from Saliva DNA
Inhibitory Effect of Norgen’s Saliva DNA Preservative on the Growth of Bacteria and Yeast
Isolation of High Quality Saliva DNA from Variable Donor Saliva Samples
Long Term Stability of DNA Stored in Norgen’s Saliva DNA Preservative
Sequencing Analysis of Saliva DNA Isolated using Norgen’s Saliva DNA Collection, Preservation and Isolation Kit
A Comparative Study Between Two Saliva DNA Preservation Systems, and Two Column-Based Saliva DNA Purification Methods
Comparison of Norgen’s Saliva DNA Collection, Preservation and Isolation Kit to a Leading Competitor’s Kit
DNA Isolation from Saliva Preserved using Norgen’s Saliva DNA Collection and Preservation Device using Beckman Coulter Agencourt
DNA Isolation from Saliva Preserved with Norgen’s Saliva DNA Collection and Preservation Device using Qiagen’s QIAamp DNA Blood
Efficient Restriction Enzyme Digestion of Saliva DNA isolated using Norgen’s Saliva DNA Collection, Preservation & Isolation Kit
Stability of DNA Stored in Norgen’s Saliva DNA Preservative for 52 Months at Room Temperature
The Use of Saliva Swabs in Norgen’s Saliva DNA Collection, Preservation and Isolation Kit
Linearity of DNA Isolated from Increasing Volumes of Preserved Saliva Using Norgen’s Saliva DNA Isolation Kit
The Effect of Elution Volume on DNA Quantity and Quality Using Norgen’s Saliva DNA Isolation Kit
Comparison of DNA Isolated from Saliva using Norgen’s Preservative and Saliva DNA Isolation Kit Vs Competitor's
Stability of DNA Stored in Norgen’s Saliva DNA Preservative at 55°C
Safety Data Sheets: 
(RU49000) Saliva DNA Collection and Preservation Devices - Safety Data Sheet
Posters: 
In‐Depth Analysis of the Microbial Diversity in Saliva Samples Preserved at Ambient Temperature for over 6 Years - PAG 2016
Rapid and qPCR ready DNA isolation from environmental and human bodily fluid samples using Magnetic bead system
Viability Of Microorganisms in Saliva & Stool Collection and Transportation Preservatives
Product Flyers: 
Saliva DNA Preservation

Title At age 9, the methylome of assisted reproductive technology children that underwent embryo culture in different media is not significantly different on a genome-wide scale
Journal Human Reproduction. 2022.
Authors Rebekka M Koeck, Florence Busato, Jorg Tost, Heleen Zandstra, Sylvie Remy, Sabine Langie, Marij Gielen, Ron van Golde, John C M Dumoulin, Han Brunner, Masoud Zamani Esteki, Aafke P A van Montfoort
Title Exposure to traffic-related air pollution and bacterial diversity in the lower respiratory tract of children
Journal PLOS ONE. 2021
Authors Niemeier-Walsh, C., Ryan, P. H., Meller, J., Ollberding, N. J., Adhikari, A., & Reponen, T.
Title Exploring influences on food choice in a large population sample: the Italian Taste Project
Journal Food Quality and Preference. 2017
Authors E. Monteleone, S. Spinelli, C. Dinnella, I. Endrizzi, M. Laureati, E. Pagliarini, F. Sinesio, F. Gasperi, L. Torri, E. Aprea, L.I. Bailetti, A. Bendini, A. Braghieri, C. Cattaneo, D. Cliceri, N. Condelli, M.C. Cravero, A. Del Caro, R. Di Monaco, S. Drago, S. Favotto, R. Fusi, L. Galassi, T. Gallina Toschi, A. Garavaldi, P. Gasparini, E. Gatti, C. Masi, A. Mazzaglia, E. Moneta, E. Piasentier, M. Piochi, N. Pirastu, S. Predieri, A. Robino, F. Russo, F. Tesini
Title Sugar-Sweetened Beverage Consumption Is Adversely Associated with Childhood Attention Deficit/Hyperactivity Disorder
Journal International Journal of Environmental Research and Public Health. 2016.
Authors Yu CJ, Du JC, Chiou HC, Feng CC, Chung MY, Yang W, Chen YS, Chien LC, Hwang B, Chen ML
Title Detection of Novel Integrons in the Metagenome of Human Saliva
Journal PLOS ONE. 2016.
Authors Tansirichaiya S, Rahman MA, Antepowicz A, Mullany P, Roberts AP
Title Increased risk of attention-deficit/hyperactivity disorder associated with exposure to organophosphate pesticide in Taiwanese children
Journal Andrology. 2016.
Authors Yu CJ, Du JC, Chiou HC, Chung MY, Yang W, Chen YS, Fuh MR, Chien LC, Hwang B, Chen ML
Title Attention Deficit/Hyperactivity Disorder and Urinary Nonylphenol Levels: A Case-Control Study in Taiwanese Children
Journal PLOS ONE. 2016.
Authors Yu CJ, Du JC, Chiou HC, Yang SH, Liao KW, Yang W, Chung MY, Chien LC, Hwang B, Chen ML
Title Functional polymorphisms in the P2X7 receptor gene are associated with stress fracture injury
Journal Purinergic Signalling. 2016.
Authors Varley I, Greeves JP, Sale C, Friedman E, Moran DS, Yanovich R, Wilson PJ, Gartland A, Hughes DC, Stellingwerff T, Ranson C, Fraser WD, Gallagher JA
Title Selective familiarity deficits in otherwise cognitively intact aging individuals with genetic risk for Alzheimer's disease
Journal Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring. 2015.
Authors Dorothee Schoemaker, Judes Poirier, Sophia Escobar, Serge Gauthier, Jens Pruessner
Title The dentin phosphoprotein repeat region and inherited defects of dentin
Journal Molecular Genetics & Genomic Medicine. 2015.
Authors Jie Yang, Kazuhiko Kawasaki, Moses Lee, Bryan M. Reid, Stephanie M. Nunez, Murim Choi, Figen Seymen, Mine Koruyucu, Yelda Kasimoglu, Ninna Estrella-Yuson, Brent P. J. Lin, James P. Simmer and Jan C.-C. Hu
Title Taurodontism, variations in tooth number, and misshapened crowns in Wnt10a null mice and human kindreds.
Journal Molecular Genetics & Genomic Medicine. 2014.
Authors Jie Yang, Shih-Kai Wang, Murim Choi, Bryan M. Reid, Yuanyuan Hu, Yuan-Ling Lee, Curtis R. Herzog, Hera Kim-Berman, Moses Lee, Paul J. Benke, K. C. Kent Lloyd, James P. Simmer and Jan C.-C. Hu.
Title Patterns of Admixture and Population Structure in Native Populations of Northwest North America.
Journal PLOS Genetics. 2014.
Authors Paul Verdu, Trevor J. Pemberton, Romain Laurent, Brian M. Kemp, Angelica Gonzalez-Oliver, Clara Gorodezky, Cris E. Hughes, Milena R. Shattuck,Barbara Petzelt, Joycelynn Mitchell, Harold Harry, Theresa William, Rosita Worl, Jerome S. Cybulski, Noah A. Rosenberg, Ripan S. Malhi.
Title Association of premenstrual/menstrual symptoms with perinatal depression and a polymorphic repeat in the polyglutamine tract of the retinoic acid induced 1 gene
Journal Journal of Affective Disorders. 2014.
Authors Ene-Choo Tan, Hui-San Tan, Tze-Ern Chua, Theresa Lee, Jasmine Ng, Ying-Chia Ch’ng, Chih-Huei Choo, Helen Y Chen
Title RANK/RANKL/OPG pathway: Genetic associations with stress fracture period prevalence in elite athletes.
Journal The Bone Journal. 2014.
Authors Ian Varley, David C. Hughes, Julie P. Greeves, Trent Stellingwerff, Craig Ranson, William D. Fraser, Craig Sale.

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