Saliva DNA Isolation 96-Well Kit (Cat. RU35200)2 x 96-well plates
Format: High Throughput
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This kit features a rapid, high-throughput 96-well format for isolating high yield and high quality DNA from saliva. The purified saliva DNA is compatible with any number of downstream applications including PCR, qPCR, Southern Blot analysis, sequencing, microarray analysis and more. This kit is also compatible with Norgen’s Saliva DNA Collection and Preservation Devices (Cat. RU49000). It is fully compatible to both vacuum and centrifugation protocols.
Human genomic DNA found in saliva can be used in various applications in diagnostics including the detection of biomarkers to diagnose a disease, follow the disease’s progress or monitor the effects of a particular treatment. Isolation of DNA from saliva for diagnostics has become an attractive alternative to isolation from blood or tissue due to the fact that sample collection is non-invasive.
Figure 1. Consistent DNA Isolation. Saliva samples were collected from 10 donors using Norgen's Saliva DNA Collection and Preservation Devices (Cat# RU49000). Norgen's Saliva Preservative was added to the saliva samples, and the 10 samples were then pooled together. Total DNA was then isolated from aliquots of the pooled and preserved saliva using columns from Norgen's Saliva DNA Isolation Kit (Cat# RU45400) or Norgen's Saliva DNA Isolation 96-Well Kit. Ten µL of each 100 µL elution from the 96-well plates were loaded on 1.2 % TAE agarose gel (Lanes 1 to 20), with the results indicating that the DNA isolation was consistent between the samples. S: DNA isolated using a single column (eluted in 200 µL). Lane M: Norgen's HighRanger 1kb DNA Ladder.
Figure 2. Real-time PCR Consistency from Saliva Samples. Saliva DNA was isolated from pooled saliva samples using Norgen's Saliva DNA Isolation 96-Well Kit and Norgen's Saliva DNA Isolation Kit (Cat# RU45400). From the 100 µL elution, 2 µL of saliva DNA was directly mixed to real-time PCR master mix (total reaction volume 20 µL), and the real-time PCR reaction was performed. The GAPDH gene was successfully amplified from the different samples without any inhibition, indicating the isolation consistency and the excellent quality of saliva DNA for downstream applications. Red: DNA samples isolated from the 96 well plate. Blue: DNA samples isolated using the single column. Black: Negative control.
Storage Conditions and Product Stability
The kit contains ready-to-use Proteinase K which is dissolved in a specially prepared storage buffer. The Proteinase K should be stored at room temperature or 4°C. All other solutions should be kept tightly sealed and stored at room temperature (15 - 25°C). This kit is stable for 1 year after the date of shipment.