Saliva DNA Isolation Kit (Magnetic Bead System)

Cat. RU55400, RU62900

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Saliva DNA Isolation Kit (Magnetic Bead System)

Cat. RU55400, RU62900

For isolating genomic DNA from preserved, and fresh saliva

Sample collection is non-invasive and painless
Fast and easy processing using a magnetic bead system
Isolate high quality genomic DNA
This kit is also compatible with Norgen’s Saliva DNA Collection and Preservation Devices
Also available in a 96-well format that can be integrated with a robotic automation system

For research use only and NOT intended for in vitro diagnostics.

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Saliva DNA Isolation Kit (Magnetic Bead System)
(Cat. RU55400) 50 preps

Saliva DNA Isolation 96-Well Kit (Magnetic Bead System)
(Cat. RU62900) 2 x 96-well plates

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Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

Kit Specifications

You have selected: Cat. RU55400

Kit Specifications
Maximum Saliva Input	0.5 mL preserved saliva 0.25 mL fresh saliva
Average Yield from 0.25 mL of Saliva*	2-5 μg
Average Purity (OD260/280)	1.6 - 1.8
Time to Complete 10 Purifications	30 minutes (Cat. RU55400) 45 minutes (Cat. RU62900)

* Average DNA yield will vary depending on the donor

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer.

You have selected: Cat. RU62900

Kit Specifications
Maximum Saliva Input	0.5 mL preserved saliva 0.25 mL fresh saliva
Average Yield from 0.25 mL of Saliva*	2-5 μg
Average Purity (OD260/280)	1.6 - 1.8
Time to Complete 10 Purifications	30 minutes (Cat. RU55400) 45 minutes (Cat. RU62900)

* Average DNA yield will vary depending on the donor

Storage Conditions and Product Stability
The kit contains ready-to-use Proteinase K which is dissolved in a specially prepared storage buffer. The Proteinase K should be stored at room temperature or 4°C. All other solutions should be kept tightly sealed and stored at room temperature (15 – 25°C). These reagents should remain stable for at least 2 years in their unopened containers.

Supporting Data

Figure 1. Resolution of DNA Isolated from Preserved Saliva Samples. DNA was isolated from 0.5 mL of preserved saliva samples collected from 6 different donors (A to F) using Norgen's Saliva DNA Isolation Kit (Magnetic Bead System). As a control, DNA was isolated from one preserved saliva sample using Norgen's Saliva DNA Isolation Kit (column format, Cat. RU45400). For evaluation, 10 µL of each 75 µL elution was run on a 1X TAE 1.2% agarose gel. Similar DNA integrity and yield were observed between the two Norgen methods, indicating the robust performance of the Saliva DNA Isolation Kit (Magnetic Bead System). Marker = Norgen's HighRanger DNA Ladder.

Figure 2. Comparison of Saliva DNA Isolation Methods (Column vs. Magnetic Bead System). DNA was isolated from 0.5 mL of preserved saliva samples collected from 6 different donors (A to F) using Norgen's Saliva DNA Isolation Kit (Magnetic Bead System). As a control, DNA was isolated from one preserved saliva sample using Norgen's Saliva DNA Isolation Kit (column format, Cat. RU45400) . All DNA purified using Norgens Saliva DNA Isolation Kit (Magnetic Bead System) [A to F] showed a comparable concentration to DNA isolated using Norgens Saliva DNA Isolation Kit (column format, Cat. RU45400), indicating the consistent and robust performance of the Saliva DNA Isolation Kit (Magnetic Bead System).

Figure 3. High Quality DNA Confirmed by Real-time PCR. DNA was isolated from 0.5 mL of preserved saliva samples collected from 6 different donors (A to F) using Norgen's Saliva DNA Isolation Kit (Magnetic Bead System). As a control, DNA was isolated from one preserved saliva sample using Norgen's Saliva DNA Isolation Kit (column format, Cat. RU45400). Saliva DNA quality was confirmed by Real-time PCR using 8 µL of saliva DNA (total PCR reaction volume was 20 µL) to detect GAPDH. GAPDH was detected from all the saliva DNA isolated using the Magnetic Bead System (blue lines), similar to Norgens column based method (Saliva DNA Isolation Kit; Cat. RU45400) (red line). The successful GAPDH detection without PCR inhibition indicates the excellent saliva DNA quality from Norgens Saliva DNA Isolation Kit (Magnetic Bead System).

Figure 4. Compatibility Test with a Competitors Saliva Collection Device. Identical saliva samples were equally divided into Norgens Saliva DNA Collection and Preservation Devices (Cat. RU49000) and Competitor Ds saliva collection device. DNA was then isolated from 0.5 mL of preserved saliva using Norgens Saliva DNA Isolation Kit (Magnetic Bead System). For evaluation, 10 µL from each 75 µL of elution were run on 1X TAE 1.2% agarose gel. For the real-time PCR, 8 µL of DNA elution was add to total 20 µL of PCR reaction to detect GAPDH (Blue: Norgen, Red: Competitor D). Marker = Norgens HighRanger DNA Ladder. The results show that Norgen's kit was able to isolate high yields of pure DNA from both collection devices, indicating the robust performance of the kit.

Figure 5. Resolution of DNA Isolated from Preserved Saliva Samples from Different Donors. DNA was isolated from 0.5 mL of preserved saliva samples collected from 8 different healthy donors using Norgen's Saliva DNA Isolation 96-Well Kit (Magnetic Bead System). For evaluation, 10 µL of each 50 µL elution was run on a 1X TAE 1.2% agarose gel. Marker = Norgen's HighRanger DNA Ladder (Cat. 11900).

Figure 6. High Quality DNA Confirmed by Real-time PCR. DNA was isolated from 0.5 mL of preserved saliva samples collected using Norgen's Saliva DNA Isolation 96-Well Kit (Magnetic Bead System). Saliva DNA quality was confirmed by Real-time PCR using 2 µL of saliva DNA (total PCR reaction volume was 20 µL) to detect GAPDH. GAPDH was detected from all the saliva DNA isolated using the Saliva DNA Isolation 96-Well Kit (Magnetic Bead System). The successful GAPDH detection without PCR inhibition indicates the excellent saliva DNA quality from Norgens Saliva DNA Isolation 96-Well Kit (Magnetic Bead System).

Click for expanded view

Component	Cat. RU55400 (50 preps)	Cat. RU62900 (192 preps)
Lysis Buffer F	30 mL	2 x 30 mL
Proteinase K in Storage Buffer	1.2 mL	4 mL
Magnetic Bead Suspension	2 x 1.1 mL	-
Elution Buffer B	8 mL	30 mL
Elution Tubes (1.7 mL)	50	-
96-Well Plate	-	2
96-Well Elution Plate	-	2
Adhesive Tape	-	2
Product Insert	1	1

Protocols:

(RU55400) Saliva DNA Isolation Kit (Magnetic Bead System) - Protocol (50 prep)

(RU62900) Saliva DNA Isolation 96-Well Kit (Magnetic Bead System) - Protocol (2 x 96-well)

Short Protocols:

(RU55400) Saliva DNA Isolation Kit (Magnetic Bead System) - Short Protocol (50 prep)

(RU62900) Saliva DNA Isolation 96-Well Kit (Magnetic Bead System) - Short Protocol (2 x 96-well)

Application Notes:

Comparison of Manual Saliva DNA Isolation to Automated Saliva DNA Isolation Using Norgen’s Magnetic Bead Saliva DNA Isolation 96-Well Kit

Product Flyers:

Saliva DNA Preservation

High Throughput Saliva DNA Isolation Kit

Product Overview
Documentation

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Saliva DNA Isolation Kit (Magnetic Bead System)

Norgen’s Saliva DNA Isolation Kit (Magnetic Bead System) provides a fast and reproducible method for isolating genomic DNA from saliva samples collected and preserved using Norgen’s Saliva DNA Collection and Preservation Devices, as well as fresh saliva. Saliva DNA purified using Norgen’s kit is of the highest quality, and is compatible with a number of downstream research applications including PCR, Southern Blot analysis, sequencing and microarray analysis.

Norgen's Saliva DNA Isolation Kit (Magnetic Bead System) is also available in a 96-well format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.

Saliva DNA Isolation Tutorial - Automated Magnetic Bead System (Cat. RU62900)

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. RU55400

Kit Specifications
Maximum Saliva Input	0.5 mL preserved saliva 0.25 mL fresh saliva
Average Yield from 0.25 mL of Saliva*	2-5 μg
Average Purity (OD260/280)	1.6 - 1.8
Time to Complete 10 Purifications	30 minutes (Cat. RU55400) 45 minutes (Cat. RU62900)

* Average DNA yield will vary depending on the donor

You have selected: Cat. RU62900

Kit Specifications
Maximum Saliva Input	0.5 mL preserved saliva 0.25 mL fresh saliva
Average Yield from 0.25 mL of Saliva*	2-5 μg
Average Purity (OD260/280)	1.6 - 1.8
Time to Complete 10 Purifications	30 minutes (Cat. RU55400) 45 minutes (Cat. RU62900)

* Average DNA yield will vary depending on the donor

Component	Cat. RU55400 (50 preps)	Cat. RU62900 (192 preps)
Lysis Buffer F	30 mL	2 x 30 mL
Proteinase K in Storage Buffer	1.2 mL	4 mL
Magnetic Bead Suspension	2 x 1.1 mL	-
Elution Buffer B	8 mL	30 mL
Elution Tubes (1.7 mL)	50	-
96-Well Plate	-	2
96-Well Elution Plate	-	2
Adhesive Tape	-	2
Product Insert	1	1