Saliva DNA Isolation Reagent Kit (up to 4 mL)
For the isolation of high quality DNA from up to 4 mL of preserved saliva samples within a laboratory setting
For research use only and NOT intended for in vitro diagnostics.
Saliva DNA Isolation Reagent Kit (up to 4 mL)
For the isolation of high quality DNA from up to 4 mL of preserved saliva samples within a laboratory setting
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Overview
- Cost effective method to isolate DNA from preserved saliva samples collected using Norgen's saliva DNA devices or other preservation methods
- Isolate DNA from a range of input volumes - up to 4 mL
- Isolate DNA of high recovery and quality suitable for sensitive downstream applications including PCR, qPCR, sequencing, SNP analysis, microarrays, RFLP and Southern Blot Analysis
Norgen's Saliva DNA Isolation Reagent Kit (up to 4 mL) is designed for isolation of high quality DNA from up to 4 mL of preserved saliva samples within a laboratory setting. The Saliva DNA Isolation Reagent Kit (up to 4 mL) contains enough reagents for 50 preps of 4 mL saliva samples preserved via Norgen Biotek's Saliva DNA Collection and Preservation Devices (RU49000), or other preservation methods.
Details
SHELF LIFE AND HANDLING
- The kit contains ready-to-use Proteinase K which is dissolved in a specially prepared storage buffer. The Proteinase K should be stored at -20°C. The Proteinase K is a white turbid solution. A white precipitate is formed upon storage. Make sure to re-suspend the white precipitate by vortexing before use.
- This kit is stable for 2 years after the date of shipment.
Component | Cat. RU35720 (50 preps) |
---|---|
Proteinase K in Storage Buffer | 8 mL |
Binding Buffer B | 85 mL |
Product Insert | 1 |
Documentation
FAQs
The procedure can be scaled down for smaller volumes. The kit manual includes a table to help with calculations.
The final DNA pellet should be resuspended in DNase-free TE buffer, which is not supplied with the kit.
To obtain a higher concentration of DNA, please use half the volume of TE buffer mentioned in calculation table (refer to product manual). It is not recommended to use lower than half the volume of TE buffer mentioned in the calculation table.
Large amounts of high molecular DNA can be very slow to rehydrate (dissolve) completely. Incubate at 55 °C for 30 minutes with occasional vortexing to aid in the process. Incomplete rehydration of the DNA will affect estimating the DNA concentration and can result in failure of downstream applications such as SNP, digestion and PCR.
Saliva DNA Isolation Reagent kit is optimized to work with saliva preserved with chemical preservatives only, for example, saliva preserved with Norgen's Saliva DNA collection and preservation devices. To isolate DNA from fresh saliva samples, please use our Saliva DNA isolation kit which can process both fresh and preserved saliva samples.
Citations
Title | Inflammatory linear verrucous epidermal nevus (ILVEN) encompasses a spectrum of inflammatory mosaic disorders |
Journal | Pediatr Dermatol. 2022. |
Authors | Atzmony L, Ugwu N, Hamilton C, Paller AS, Zech L, Antaya RJ, Choate KA |
Title | CYP3A genetic variation and taxane-induced peripheral neuropathy: a systematic review, meta-analysis, and candidate gene study |
Journal | Front Pharmacol. 2023. |
Authors | McEvoy L, Cliff J, Carr DF, Jorgensen A, Lord R, Pirmohamed M |
Title | Second-Hit, Postzygotic PMVK and MVD Mutations in Linear Porokeratosis |
Journal | JAMA Dermatol. 2019. |
Authors | Atzmony L, Khan HM, Lim YH, Paller AS, Levinsohn JL, Holland KE, Mirza FN, Yin E, Ko CJ, Leventhal JS, Choate KA |
Title | Alterations to the gut microbiome after sport-related concussion in a collegiate football players cohort: A pilot study |
Journal | Brain Behav Immun Health. 2022. |
Authors | Soriano S, Curry K, Sadrameli SS, Wang Q, Nute M, Reeves E, Kabir R, Wiese J, Criswell A, Schodrof S, Britz GW, Gadhia R, Podell K, Treangen T, Villapol S |