Soil DNA Isolation Kit (Magnetic Bead System)

Cat. 58100, 62800

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Soil DNA Isolation Kit (Magnetic Bead System)

Cat. 58100, 62800

For isolating genomic DNA from soil samples

Fast and easy processing using a magnetic bead system
Robust lysis system (chemical lysis combined with a mechanical homogenization)
Isolate high quality genomic DNA
High yields - Consistent, high yields of inhibitor-free DNA up to 50 kb plus
Isolate sequencing quality total DNA from a variety of microorganisms including bacteria, fungi and algae
Also available in a 96-well format that can be integrated with a robotic automation system

For research use only and NOT intended for in vitro diagnostics.

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Soil DNA Isolation Kit (Magnetic Bead System)
(Cat. 58100) 50 preps

Soil DNA Isolation 96-Well Kit (Magnetic Bead System)
(Cat. 62800) 2 x 96-well plates

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Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

Kit Specifications

You have selected: Cat. 58100

Kit Specifications
Maximum Soil Input	0.25 g
Type of Soil Processed	All types of soil
Average Yield from 0.25 mL of Soil*	1 - 5 μg
Average Purity (OD260/280)	1.7 - 1.8
Time to Complete Purifications	40 minutes (Cat. 58100) 50 minutes (Cat. 62800)

* Average DNA yields will vary depending upon soil sample types

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

You have selected: Cat. 62800

Kit Specifications
Maximum Soil Input	0.25 g
Type of Soil Processed	All types of soil
Average Yield from 0.25 mL of Soil*	1 - 5 μg
Average Purity (OD260/280)	1.7 - 1.8
Time to Complete Purifications	40 minutes (Cat. 58100) 50 minutes (Cat. 62800)

* Average DNA yields will vary depending upon soil sample types

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Supporting Data

Figure 1. Resolution of DNA isolated from two different types of soil samples DNA was isolated from high humic acid soil (top soil) and regular soil (clay) using Norgens column-based Soil DNA Isolation Kit (Red C) and Norgen's Soil DNA Isolation Kit (Magnetic Bead System) (Mag Bead). For evaluation, 10 µL from the 75 µL elution was run on 1X TAE 1.2% agarose gel. Excellent DNA integrity and yield were observed from the Soil DNA Isolation Kit (Magnetic Bead System), indicating the robust performance comparable to the column based method. Marker = Norgens HighRanger DNA Ladder.

Figure 2. Two of Norgens soil DNA isolation methods (Column vs Magnetic Bead System) were compared for DNA concentration. All DNA elutions isolated using Norgens Soil DNA Isolation Kit (Magnetic Bead System) [A to E] showed a comparable DNA concentration to Norgens Soil DNA Isolation Kit (Column Method; Cat. 26500), indicating the consistent and robust performance of the Soil DNA Isolation Kit (Magnetic Bead System).

Figure 3. High soil DNA quality was confirmed by real-time PCR using 8 µL of soil DNA (total PCR reaction volume was 20 µL) isolated using Norgens Soil DNA Isolation Kit (Magnetic Bead System) to detect 16s rDNA. PCR results from Norgen's Soil DNA Isolation Kit (Magnetic Bead System) (circle) were comparable to Norgens column based Soil DNA Isolation Kit (cross) and showed the successful 16s rDNA detection without PCR inhibition, indicating the excellent soil DNA quality using Norgens Soil DNA Isolation Kit (Magnetic Bead System).

Figure 4. Hierarchical Clustering Dendrogram. High quality of soil DNA was successfully isolated from the 5 challenging soil types using Norgen's Soil DNA Isolation Kits (A: Soil DNA Isolation Kit (Magnetic Bead System) and B: Soil DNA Isolation Kit Spin Column) respectively. The Hierarchical Clustering Dendrogram is based on genus-level classifications and shows the relative abundance of its genus-level classifications among soil types.

Figure 5. Principal Coordinate Analysis (PCoA) generated by Illumina MiSeq. Principal Coordinate Analysis of 20 samples (25-28: Mulch, 29-32: Clay, 33-36: Potting soil, 76-78: Top soil, 79-82: Compost) showing differences in the distribution of taxonomic classifications between samples up to species level. Each soil type is clearly clustered together and away from other types.

Figure 6. Resolution of DNA Isolated from Top Soil. DNA was isolated from high humic acid soil (top soil) using Norgen's Soil DNA Isolation 96-Well Kit (Magnetic Bead System). For evaluation, 10 µL from the 75 µL elution was run on 1X TAE 1.2% agarose gel. Excellent DNA integrity, consistency and yield were observed from the Soil DNA Isolation 96-Well Kit (Magnetic Bead System). Marker = Norgens HighRanger DNA Ladder (Cat. 11900).

Figure 7. High Soil DNA Quality Was Confirmed By Real-time PCR Using 2 µL of Soil DNA (Total PCR Reaction Volume Was 20 µL) Isolated Using Norgens Soil DNA Isolation 96-Well Kit (Magnetic Bead System) to Detect 16s rRNA. PCR results from Norgen's Soil DNA Isolation 96-Well Kit (Magnetic Bead System) showed the successful 16s rRNA detection without PCR inhibition, indicating the excellent soil DNA quality using Norgens Soil DNA Isolation 96-Well Kit (Magnetic Bead System).

Click for expanded view

Protocols:

(58100) Soil DNA Isolation Kit (Magnetic Bead System) - Protocol (50 prep)

(62800) Soil DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System) - Protocol (2 x 96-well)

Safety Data Sheets:

58100, 62800 - Safety Data Sheet

Product Flyers:

Soil DNA Isolation Kit (Magnetic Bead System)

Product Overview
Documentation

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Soil DNA Isolation Kit (Magnetic Bead System)

Norgen’s Soil DNA Isolation Kit (Magnetic Bead System) provides a fast and reproducible method for isolating genomic DNA from soil samples. All types of soil samples can be processed with this kit, including common soil samples and difficult soil samples with high humic acid content such as compost and manure. The kit removes all traces of humic acid using the provided the OSR (Organic Substance Removal) Solution. Total genomic DNA can be isolated and purified from all the various microorganisms found in soil, such as bacteria, fungi and algae. The purified DNA is of the highest quality and is fully compatible with downstream PCR applications, as all humic acid substances and PCR inhibitors are removed during the isolation.

Norgen's Soil DNA Isolation Kit (Magnetic Bead System) is also available in a 96-well format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 58100

Kit Specifications
Maximum Soil Input	0.25 g
Type of Soil Processed	All types of soil
Average Yield from 0.25 mL of Soil*	1 - 5 μg
Average Purity (OD260/280)	1.7 - 1.8
Time to Complete Purifications	40 minutes (Cat. 58100) 50 minutes (Cat. 62800)

* Average DNA yields will vary depending upon soil sample types

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

You have selected: Cat. 62800

Kit Specifications
Maximum Soil Input	0.25 g
Type of Soil Processed	All types of soil
Average Yield from 0.25 mL of Soil*	1 - 5 μg
Average Purity (OD260/280)	1.7 - 1.8
Time to Complete Purifications	40 minutes (Cat. 58100) 50 minutes (Cat. 62800)

* Average DNA yields will vary depending upon soil sample types

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Protocols:

(58100) Soil DNA Isolation Kit (Magnetic Bead System) - Protocol (50 prep)

(62800) Soil DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System) - Protocol (2 x 96-well)

Safety Data Sheets:

58100, 62800 - Safety Data Sheet

Product Flyers:

Soil DNA Isolation Kit (Magnetic Bead System)