Soil DNA Isolation Kit (Magnetic Bead System)
For isolating genomic DNA from soil samples
For research use only and NOT intended for in vitro diagnostics.
Soil DNA Isolation Kit (Magnetic Bead System)
For isolating genomic DNA from soil samples
Overview
- Fast and easy processing using a magnetic bead system
- Robust lysis system (chemical lysis combined with a mechanical homogenization)
- Isolate high quality genomic DNA
- High yields - Consistent, high yields of inhibitor-free DNA up to 50 kb plus
- Isolate sequencing quality total DNA from a variety of microorganisms including bacteria, fungi and algae
- Also available in a 96-well format that can be integrated with a robotic automation system
Norgen’s Soil DNA Isolation Kit (Magnetic Bead System) provides a fast and reproducible method for isolating genomic DNA from soil samples. All types of soil samples can be processed with this kit, including common soil samples and difficult soil samples with high humic acid content such as compost and manure. The kit removes all traces of humic acid using the provided the OSR (Organic Substance Removal) Solution. Total genomic DNA can be isolated and purified from all the various microorganisms found in soil, such as bacteria, fungi and algae. The purified DNA is of the highest quality and is fully compatible with downstream PCR applications, as all humic acid substances and PCR inhibitors are removed during the isolation.
Norgen's Soil DNA Isolation Kit (Magnetic Bead System) is also available in a 96-well format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.
Details
Supporting Data
Kit Specifications
|
|
Maximum Soil Input
|
0.25 g
|
Type of Soil Processed
|
All types of soil
|
Average Yield from 0.25 mL of Soil*
|
1 - 5 μg
|
Average Purity (OD260/280) |
1.7 - 1.8
|
Time to Complete Purifications |
40 minutes (Cat. 58100)
50 minutes (Cat. 62800) |
* Average DNA yields will vary depending upon soil sample types
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
Component | Cat. 58100 (50 preps) | Cat. 62800 (2 x 96-well) |
---|---|---|
Lysis Buffer G | 100 mL | 1 x 100 mL 1 x 45 mL |
Lysis Additive A | 6 mL | 25 mL |
Binding Buffer I | 7 mL | 25 mL |
OSR Solution | 3 mL | 12 mL |
Binding Buffer B | 2 x 12 mL | 85 mL |
Magnetic Bead Suspension | 2 x 1.1 mL | 8.5 mL |
Buffer SK | 30 mL |
1 x 40 mL 1 x 60 mL |
Elution Buffer B | 8 mL | 15 mL |
Elution Tubes (1.7 mL) | 50 | - |
Bead Tubes | 50 | 196 |
96-Well Plate | - | 2 |
96-Well Elution Plate | - | 2 |
Adhesive Tape | - | 2 |
Product Insert | 1 | 1 |