Staphylococcus aureus TaqMan PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Staphylococcus aureus TaqMan PCR Detection Kits Price USD$699.00 SKU TM29350 Format PCR Probe/Primer and Control Set Size 100 Reactions Quantity Overview Details Documentation Citations Overview Detection kits for Staphylococcus aureus Available in TaqMan format for analysis Mastitis is the single most costly disease of dairy cattle resulting in the reduction of milk yield and quality. The inflammation of the utter is mainly caused by bacteria, and Staphylococcus aureus is often considered the most common cause of contagious mastitis in dairy herds. S. aureus infection is estimated to be present in up to 90% of dairy farms and is responsible for 35% of the economic loss in the dairy industry (Lee et al., 2005). S. aureus is a facultatively anaerobic, Gram positive bacterium. The majority of S. aureus strains are catalase-positive and coagulasepositive, which forms the basis of traditional identification methodology. TaqMan PCR Kit Click to expand options Staphylococcus aureus TaqMan PCR Kit, 100 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents Staphylococcus aureus TaqMan PCR Probe/Primer Set and Controls, 100 reactions Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest Nuclease-free water Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix For research use only and NOT intended for in vitro diagnostics. Details Supporting Data Previous TaqMan PCR Kit - Figure 1. Example of TaqMan PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 2. Example of TaqMan PCR Negative result. No target DNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR. TaqMan PCR Kit - Figure 3. Example of TaqMan PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification. Next Click for expanded view Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. Component Cat. TM29350 (100 preps) Cat. TM29310 (100 preps) MDx TaqMan 2X PCR Master Mix 2 x 700 μL - S. aureus Primer & Probe Mix 280 μL 280 μL S. aureus Positive Control 150 μL 150 μL Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL Product Insert 1 1 Documentation PROTOCOLS (TM29350) Staphylococcus aureus TaqMan PCR Kit - Protocol (100 rxns) (TM29310) Staphylococcus aureus TaqMan Probe/Primer and Control Set - Protocol (100 rxns) SAFETY DATA SHEETS (TM29350) Staphylococcus aureus TaqMan PCR Kit - Safety Data Sheet Related Products Staphylococcus aureus TaqMan Probe/Primer and Control Set Milk Bacterial DNA Isolation Kit Milk Bacterial DNA Isolation Kit
Staphylococcus aureus TaqMan PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Staphylococcus aureus TaqMan PCR Detection Kits Price USD$699.00 SKU TM29350 Format PCR Probe/Primer and Control Set Size 100 Reactions Quantity
