Stool Nucleic Acid Collection and Preservation Devices Dx

Cat. Dx45660

Stool nucleic acid collection and preservation tubes for diagnostic use

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What Scientists are Saying About this Product

We have been using Norgen's using the Stool Nucleic Acid Collection and Preservation Tubes and Stool Total RNA Purification Kit for many years for our projects focused on searching fecal biomarkers (small non-coding RNAs and bacterial species) to diagnose colorectal cancer non-invasively. The challenge was to collect stool samples and preserve nucleic acids in a way that patients could collect samples at home and bring to the receiving hospital without rush and preserving the samples. We successfully used the collection tubes for over 1000 samples in the frame of an European project and we were able to extract either RNA and microbial DNA for metagenome analysis.

Barbara Pardini, Italian Institute for Genomic Medicine

Stool Nucleic Acid Collection and Preservation Devices Dx

Cat. Dx45660

For the collection and preservation of stool samples at ambient temperatures

CE-IVDR marked in accordance with the European Commission Regulation (EU) No. 2017/746.
Ideal for use in in vitro workflows
No need to immediately process samples
Nucleic acid preservation at room temperature over 2 years for DNA and 7 days for RNA
Ship stool samples at room temperature safely
Compatible with most DNA/RNA isolation methods

Intended for in vitro diagnostics use.

Research version available here

Note: This product is not available for sale in the United States

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Stool Nucleic Acid Collection and Preservation Devices Dx
(Cat. Dx45660) 50 Devices

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Product Size

Kit Specifications

You have selected: Cat. Dx45660

Kit Specifications
Stool Input	2 g
Stability of Stool Nucleic Acids at Room Temperature	2 years for DNA 7 days for RNA*

* The RNA stability will be vary depending on the samples

Supporting Data

Figure 1. Norgen's Best-In-Class Preservation. Summary of community shifts in response to stabilizing solutions over a 14-day storage period. Bray-Curtis distance towards the reference for each patient grouped by stabilizing solution. Median and 5th-to-95th percentile ranges are shown for both interaliquot and interpatient variability (adapted from Plauzolles et al, 2022). Norgen is the most similar to frozen stool samples, over 11 other sample collection and storage methods..

Figure 2. Integrity of Total Stool DNA and RNA Isolated from Preserved Stool Samples. DNA and RNA were isolated from 200 µL of preserved stool from 4 different donors (lanes A to D) stored at room temperature at the indicated time points (DNA at 27 months and RNA at 7 days) using Norgen's Stool Nucleic Acid Isolation Kit (Cat# 45600). Next, 10 µL from the 75 µL eluted nucleic acid was loaded onto a gel for visual analysis. High integrity stool DNA was isolated from preserved stool samples that were stored at room temperature for 27 months, and high integrity RNA was isolated from stool samples stored for 7 days at ambient temperature. Lane M is Norgen's HighRanger 1 kb DNA Ladder (Cat. 11900).

Figure 3. Monitoring the DNA stability of Adenovirus (Ad5subE1) Spiked into Preserved Stool Samples using TaqMan Real-Time PCR Assay.Preserved stool samples werespiked with 6 x 10⁹ pfu/mL of Adenovirus, and 200 µL samples were removed at the indicated time points. The stool DNA was then isolated using Norgen's Stool DNA Isolation Kit (Cat# 27600). Next, 2 µL of DNA template from each 50 µL of elution was used in a 20 µL PCR reaction to detect the Adenovirus DNA. The stool preservative was shown to stabilize Adenovirus DNA up to 21 weeks at ambient temperature, indicating that Norgen's Stool Nucleic Acid Collection and Preservation Tubes provide a safe and convenient sample transportation system.

Figure 4. Stool DNA is Stable over a Wide Range of Temperatures. Stool was collected from a healthy donor using Norgen's Stool Nucleic AcidCollection andPreservation Tubes. Samples were then incubated at ambient temperature (Fig. 3A) and 50°C (Fig. 3B) respectively. Next, 200 µL of the preserved and non-preserved stool was taken at day 1, 2, 4 and 7, and DNA was isolated using Norgen's Stool DNA Isolation Kit (Cat.# 27600). Lastly, 2 µL of elution was used in 20 µL of the real-time PCR reaction (SYBR Green I) using CFX96 Touch Real-Time PCR Detection System (Bio-Rad). The preserved stool generated lower Ct values when compared to the non-preserved stool at the various time points tested, indicating the high quality of the preserved DNA.

Figure 5. Stability of Human and Bacterial Genes over a 7 Day Period. Human (GAPDH), Gram-negative (16S rDNA) and two gastrointestinal Gram-positive bacterial genes were monitored from stool samples stored at ambient temperature with and without Norgen's stool preservative. DNA was isolated from 200 µL of preserved, non-preserved, and fresh stool samples on day 1, day 2, day 4 and day 7 using Norgen's Stool DNA Isolation Kit (Cat.# 27600). Next, 2 µL of elution was used in 20 µL of the real-time PCR reaction (SYBR Green I) using CFX96 Touch Real-Time PCR Detection System (Bio-Rad). All of the genes were shown to be preserved and stable over 7 days.

Figure 6. Hierarchical Clustering of Stool Samples at Phylum Level Under Different Storage Conditions. The microbial composition from the stool samples preserved in Norgens Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45630, 45660, Dx45660) did not differ significantly to that of controls (fresh stool from Day 0 at RT) over a 2 day period while non-preserved stool samples showed a distance in the dendrogram from the original stool profile.

Figure 7. Difference In Top 5 Abundant Microorganisms at Phylum Level Compared to Fresh Stool (Day 0). Abundance of Firmicuties, Bacteroidetes and proteobacteria from the preserved stool sample was higher than non-preserved (NP) to the fresh stool after 2 days storage at RT, indicating that the originality of microorganism profile from the preserved are much closer to the fresh stool sample (Day 0).

Figure 8. Relative Abundance of Bacterial Genera Between Preserved And Non-preserved Stool Sample Stored at RT For 2 Days. Preserved stool sample using Norgens Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45630, 45660,Dx45660) showed closed relativeness to the fresh stool sample (Day 0).

Figure 9. Correlation between duplicate bacterial metagenomic reads using (A) Fresh stool (day 0) and preserved stool at RT for 2 days and (B) Fresh stool (day 0) and non-preserved stool at RT for 2 days. A greater R2 was observed for Fresh stool (day 0) and preserved stool, suggestive of higher consistency between fresh stool sample and preserved stool sample

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Protocols:

(Dx45660) Stool Nucleic Acid Collection and Preservation Devices Dx - Protocol (50 devices)

Application Notes:

Detection of Viral Bacterial and Human Genomic DNA From Preserved Stool Samples - Application Note

Purification and Detection of Bacterial RNA From Preserved Stool Samples - Application Note

Immediate Viral Inactivation Using Norgen’s Stool Nucleic Acid Preservative

Gut Microbiome Diversity: Comparison of Stool DNA Preservation Methods

Posters:

Effect of Stool Transportation Conditions On Microbiota Diversity

Viability Of Microorganisms in Saliva & Stool Collection and Preservation Preservatives

Product Flyers:

Stool Nucleic Acid Collection and Preservation System

Product Overview
Documentation

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Stool Nucleic Acid Collection and Preservation Devices Dx

Norgen’s Stool Nucleic Acid Collection and Preservation Devices Dx are designed for the collection and preservation of nucleic acids from fresh stool specimens. The Stool Nucleic Acid Collection and Preservation Devices Dx - 50 contains 50 individual Stool Nucleic Acid Collection and Preservation Devices Dx. Each Stool Nucleic Acid Collection and Preservation Device Dx consists of Norgen’s Stool Nucleic Acid Collection and Preservation Tube Dx containing Norgen’s Stool Preservative in a liquid format. The user simply collects stool into the tubes (fill up to the line indicated on the tube) and mixes gently until the stool is well submerged under the preservative. The Stool Nucleic Acid Collection and Preservation Tube Dx is subsequently sent to the laboratory for DNA and/or RNA isolation and analysis. The stool DNA in preserved samples is stable for more than 2 years at room temperature, and the stool RNA in preserved samples is stable for 7 days at room temperature. DNA can be isolated from the preserved stool samples using Norgen’s Stool DNA Isolation Kit Dx (Cat. Dx27600) and RNA can be isolated from the preserved stool samples using Norgen’s Stool Total RNA Purification Kit Dx (Cat. Dx49500). These tubes are ideal for collecting and preserving DNA and RNA samples for in vitro diagnostic use for medical purposes.

NOTE: This product is not available for sale in the United States.

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