Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45630, 45660)
Stool Nucleic Acid Collection and Preservation Tubes
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Stool Nucleic Acid Collection and Preservation Tubes
A rapid and simple method for the collection, preservation and room temperature shipping of stool samples.
- The preservative provides sample homogeneity eliminating sample variability
- Preserve and transport DNA & RNA safely at ambient temperature
- No cold shipping/storage needed - hassle-free and cost effective
- Isolate nucleic acids for any application including 16s NGS
- Robust preservation over a range of temperatures
- Customizable with various accessories for easy and safe collection
- Eliminates odor and renders samples safe and non-infectious
For research use only and NOT intended for in vitro diagnostics.
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(Cat. 45630) 1 tube
(Cat. 45660) 50 Tubes
Kit Size
- The preservative provides sample homogeneity eliminating sample variability
- Preserve and transport DNA & RNA safely at ambient temperature
- No cold shipping/storage needed - hassle-free and cost effective
- Isolate nucleic acids for any application including 16s NGS
- Robust preservation over a range of temperatures
- Customizable with various accessories for easy and safe collection
- Eliminates odor and renders samples safe and non-infectious
Stool Nucleic Acid Collection and Preservation Tubes
Norgen’s Stool Nucleic Acid Collection and Preservation Tubes are designed for the rapid preservation of nucleic acids from fresh stool specimens. The Stool Nucleic Acid Collection and Preservation Tubes contain Norgen’s Stool Preservative in a liquid format. The user simply collects stool into the tubes (fill up to the line indicated on the tube) and mixes gently until the stool is well submerged under the preservative. The Stool Preservative prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the Stool Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the Stool Preservative allow samples to be stored at room temperature for over 2 years for DNA and 7 days for RNA. To extend the stool RNA stability in the preservative, storage at -20°C or -70°C is recommended at the arrival to testing facilities until the RNA purification.
Infographic
Kit Specifications
|
Kit Specifications
|
|
|
Stool Input
|
2 g
|
| Stability of Stool Nucleic Acids at Room Temperature |
2 years for DNA
7 days for RNA* |
* The RNA stability will vary depending on the samples
Storage Conditions and Product Stability
All tubes should be kept tightly sealed and stored at room temperature (15 – 25 °C) for up to 2 years without any reduction in performance.
|
Kit Specifications
|
|
|
Stool Input
|
2 g
|
| Stability of Stool Nucleic Acids at Room Temperature |
2 years for DNA
7 days for RNA* |
* The RNA stability will vary depending on the samples
Storage Conditions and Product Stability
All tubes should be kept tightly sealed and stored at room temperature (15 – 25 °C) for up to 2 years without any reduction in performance.
Supporting Data
Figure 1. Norgen's Best-In-Class Preservation. Summary of community shifts in response to stabilizing solutions over a 14-day storage period. Bray-Curtis distance towards the reference for each patient grouped by stabilizing solution. Median and 5th-to-95th percentile ranges are shown for both interaliquot and interpatient variability (adapted from Plauzolles et al, 2022). Norgen is the most similar to frozen stool samples, over 11 other sample collection and storage methods.
Figure 2. Integrity of Total Stool DNA and RNA Isolated from Preserved Stool Samples. DNA and RNA were isolated from 200 µL of preserved stool from 4 different donors (lanes A to D) stored at room temperature at the indicated time points (DNA at 27 months and RNA at 7 days) using Norgen's Stool Nucleic Acid Isolation Kit (Cat# 45600). Next, 10 µL from the 75 µL eluted nucleic acid was loaded onto a gel for visual analysis. High integrity stool DNA was isolated from preserved stool samples that were stored at room temperature for 27 months, and high integrity RNA was isolated from stool samples stored for 7 days at ambient temperature. Lane M is Norgen's HighRanger 1 kb DNA Ladder (Cat. 11900).
Figure 3. Monitoring the DNA stability of Adenovirus (Ad5subE1) Spiked into Preserved Stool Samples using TaqMan Real-Time PCR Assay.Preserved stool samples werespiked with 6 x 109 pfu/mL of Adenovirus, and 200 µL samples were removed at the indicated time points. The stool DNA was then isolated using Norgen's Stool DNA Isolation Kit (Cat# 27600). Next, 2 µL of DNA template from each 50 µL of elution was used in a 20 µL PCR reaction to detect the Adenovirus DNA. The stool preservative was shown to stabilize Adenovirus DNA up to 21 weeks at ambient temperature, indicating that Norgen's Stool Nucleic Acid Collection and Preservation Tubes provide a safe and convenient sample transportation system.
Figure 4. Hierarchical Clustering of Stool Samples at Phylum Level Under Different Storage Conditions. The microbial composition from the stool samples preserved in Norgens Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45630, 45660) did not differ significantly to that of controls (fresh stool from Day 0 at RT) over a 2 day period while non-preserved stool samples showed a distance in the dendrogram from the original stool profile.
Figure 5. Difference In Top 5 Abundant Microorganisms at Phylum Level Compared to Fresh Stool (Day 0). Abundance of Firmicuties, Bacteroidetes and proteobacteria from the preserved stool sample was higher than non-preserved (NP) to the fresh stool after 2 days storage at RT, indicating that the originality of microorganism profile from the preserved are much closer to the fresh stool sample (Day 0).
Figure 6. Relative Abundance of Bacterial Genera Between Preserved And Non-preserved Stool Sample Stored at RT For 2 Days. Preserved stool sample using Norgens Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45630, 45660) showed closed relativeness to the fresh stool sample (Day 0).
Figure 7. Correlation between duplicate bacterial metagenomic reads using (A) Fresh stool (day 0) and preserved stool at RT for 2 days and (B) Fresh stool (day 0) and non-preserved stool at RT for 2 days. A greater R2 was observed for Fresh stool (day 0) and preserved stool, suggestive of higher consistency between fresh stool sample and preserved stool sample
| Component | Cat. 45660 |
|---|---|
| Stool Nucleic Acid Collection and Preservation Tubes | 50 |
| Product Insert | 1 |
| Title | Impact of probiotic supplementation on exercise endurance among non-elite athletes: study protocol for a randomized, placebo-controlled, double-blind, clinical trial |
| Journal | Trials. 2022. |
| Authors | Caitlin E. McDermott, Heather K. Vincent, Anne E. Mathews, Brunella Gonzalez Cautela, Mariana Sandoval, Annie Tremblay & Bobbi Langkamp-Henken |
| Title | Effects of Inulin-Based Prebiotics Alone or in Combination with Probiotics on Human Gut Microbiota and Markers of Immune System: A Randomized, Double-Blind, Placebo-Controlled Study in Healthy Subjects |
| Journal | Microorganisms. 2022. |
| Authors | Alessandra De Giani, Anna Sandionigi, Jessica Zampolli, Angela Michelotti, Francesco Tursi, Massimo Labra and Patrizia Di Gennaro |
| Title | Effectiveness of Multistrain Probiotic Formulation on Common Infectious Disease Symptoms and Gut Microbiota Modulation in Flu-Vaccinated Healthy Elderly Subjects |
| Journal | BioMed Research International. 2022. |
| Authors | Anna Sandionigi, Alessandra De Giani, Francesco Tursi, Angela Michelotti, Enza Cestone, Silvana Giardina, Jessica Zampolli, and Patrizia Di Gennaro |
| Title | Development of a rational framework for the therapeutic efficacy of fecal microbiota transplantation for calf diarrhea treatment |
| Journal | Microbiome. 2022. |
| Authors | Islam J, Tanimizu M, Shimizu Y, Goto Y, Ohtani N, Sugiyama K, Tatezaki E, Sato M, Makino E, Shimada T, Ueda C, Matsuo A, Suyama Y, Sakai Y, Furukawa M, Usami K, Yoneyama H, Aso H, Tanaka H, Nochi T |
| Title | Human Stool Preservation Impacts Taxonomic Profiles in 16S Metagenomics Studies |
| Journal | Frontiers in Cellular and Infection Microbiology. 2020. |
| Authors | A. Plauzolles, E. Toumi, M. Bonnet, G. Pénaranda, G. Bidaut, L. Chiche, J. Allardet-Servent, F. Retornaz, B. Goutorbe, & P. Halfon |
| Title | Air pollution exposure is associated with the gut microbiome as revealed by shotgun metagenomic sequencing |
| Journal | Journal of Environmental Management. 2020. |
| Authors | Farnaz Fouladi, Maximilian J. Bailey, William B. Patterson, Michael Sioda, Ivory C. Blakley, Anthony A. Fodor, Roshonda B. Jones, Zhanghua Chen, Jeniffer S. Kim, Frederick Lurmann, Cameron Martino, Rob Knight, Frank D. Gilliland, Tanya L. Alderete |
| Title | Altered Fecal Small RNA Profiles in Colorectal Cancer Reflect Gut Microbiome Composition in Stool Samples |
| Journal | ASM Journals. 2019. |
| Authors | Sonia Tarallo, Giulio Ferrero, Gaetano Gallo, Antonio Francavilla, Giuseppe Clerico, Alberto Realis Luc, Paolo Manghi, Francesca Cordero |
| Title | Bacteroidales markers for microbial source tracking in Southeast Asia |
| Journal | Water Research. 2017. |
| Authors | Jean Pierre Nshimyimana, Mercedes C. Cruz, R. Janelle Thompson, Stefan Wuertz |
Stool Nucleic Acid Collection and Preservation Tubes
Norgen’s Stool Nucleic Acid Collection and Preservation Tubes are designed for the rapid preservation of nucleic acids from fresh stool specimens. The Stool Nucleic Acid Collection and Preservation Tubes contain Norgen’s Stool Preservative in a liquid format. The user simply collects stool into the tubes (fill up to the line indicated on the tube) and mixes gently until the stool is well submerged under the preservative. The Stool Preservative prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the Stool Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the Stool Preservative allow samples to be stored at room temperature for over 2 years for DNA and 7 days for RNA. To extend the stool RNA stability in the preservative, storage at -20°C or -70°C is recommended at the arrival to testing facilities until the RNA purification.
Infographic
Supporting Data
Figure 1. Norgen's Best-In-Class Preservation. Summary of community shifts in response to stabilizing solutions over a 14-day storage period. Bray-Curtis distance towards the reference for each patient grouped by stabilizing solution. Median and 5th-to-95th percentile ranges are shown for both interaliquot and interpatient variability (adapted from Plauzolles et al, 2022). Norgen is the most similar to frozen stool samples, over 11 other sample collection and storage methods.
Figure 2. Integrity of Total Stool DNA and RNA Isolated from Preserved Stool Samples. DNA and RNA were isolated from 200 µL of preserved stool from 4 different donors (lanes A to D) stored at room temperature at the indicated time points (DNA at 27 months and RNA at 7 days) using Norgen's Stool Nucleic Acid Isolation Kit (Cat# 45600). Next, 10 µL from the 75 µL eluted nucleic acid was loaded onto a gel for visual analysis. High integrity stool DNA was isolated from preserved stool samples that were stored at room temperature for 27 months, and high integrity RNA was isolated from stool samples stored for 7 days at ambient temperature. Lane M is Norgen's HighRanger 1 kb DNA Ladder (Cat. 11900).
Figure 3. Monitoring the DNA stability of Adenovirus (Ad5subE1) Spiked into Preserved Stool Samples using TaqMan Real-Time PCR Assay.Preserved stool samples werespiked with 6 x 109 pfu/mL of Adenovirus, and 200 µL samples were removed at the indicated time points. The stool DNA was then isolated using Norgen's Stool DNA Isolation Kit (Cat# 27600). Next, 2 µL of DNA template from each 50 µL of elution was used in a 20 µL PCR reaction to detect the Adenovirus DNA. The stool preservative was shown to stabilize Adenovirus DNA up to 21 weeks at ambient temperature, indicating that Norgen's Stool Nucleic Acid Collection and Preservation Tubes provide a safe and convenient sample transportation system.
Figure 4. Hierarchical Clustering of Stool Samples at Phylum Level Under Different Storage Conditions. The microbial composition from the stool samples preserved in Norgens Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45630, 45660) did not differ significantly to that of controls (fresh stool from Day 0 at RT) over a 2 day period while non-preserved stool samples showed a distance in the dendrogram from the original stool profile.
Figure 5. Difference In Top 5 Abundant Microorganisms at Phylum Level Compared to Fresh Stool (Day 0). Abundance of Firmicuties, Bacteroidetes and proteobacteria from the preserved stool sample was higher than non-preserved (NP) to the fresh stool after 2 days storage at RT, indicating that the originality of microorganism profile from the preserved are much closer to the fresh stool sample (Day 0).
Figure 6. Relative Abundance of Bacterial Genera Between Preserved And Non-preserved Stool Sample Stored at RT For 2 Days. Preserved stool sample using Norgens Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45630, 45660) showed closed relativeness to the fresh stool sample (Day 0).
Figure 7. Correlation between duplicate bacterial metagenomic reads using (A) Fresh stool (day 0) and preserved stool at RT for 2 days and (B) Fresh stool (day 0) and non-preserved stool at RT for 2 days. A greater R2 was observed for Fresh stool (day 0) and preserved stool, suggestive of higher consistency between fresh stool sample and preserved stool sample
Kit Specifications
|
Kit Specifications
|
|
|
Stool Input
|
2 g
|
| Stability of Stool Nucleic Acids at Room Temperature |
2 years for DNA
7 days for RNA* |
* The RNA stability will vary depending on the samples
Storage Conditions and Product Stability
All tubes should be kept tightly sealed and stored at room temperature (15 – 25 °C) for up to 2 years without any reduction in performance.
|
Kit Specifications
|
|
|
Stool Input
|
2 g
|
| Stability of Stool Nucleic Acids at Room Temperature |
2 years for DNA
7 days for RNA* |
* The RNA stability will vary depending on the samples
Storage Conditions and Product Stability
All tubes should be kept tightly sealed and stored at room temperature (15 – 25 °C) for up to 2 years without any reduction in performance.
| Component | Cat. 45660 |
|---|---|
| Stool Nucleic Acid Collection and Preservation Tubes | 50 |
| Product Insert | 1 |
| Title | Impact of probiotic supplementation on exercise endurance among non-elite athletes: study protocol for a randomized, placebo-controlled, double-blind, clinical trial |
| Journal | Trials. 2022. |
| Authors | Caitlin E. McDermott, Heather K. Vincent, Anne E. Mathews, Brunella Gonzalez Cautela, Mariana Sandoval, Annie Tremblay & Bobbi Langkamp-Henken |
| Title | Effects of Inulin-Based Prebiotics Alone or in Combination with Probiotics on Human Gut Microbiota and Markers of Immune System: A Randomized, Double-Blind, Placebo-Controlled Study in Healthy Subjects |
| Journal | Microorganisms. 2022. |
| Authors | Alessandra De Giani, Anna Sandionigi, Jessica Zampolli, Angela Michelotti, Francesco Tursi, Massimo Labra and Patrizia Di Gennaro |
| Title | Effectiveness of Multistrain Probiotic Formulation on Common Infectious Disease Symptoms and Gut Microbiota Modulation in Flu-Vaccinated Healthy Elderly Subjects |
| Journal | BioMed Research International. 2022. |
| Authors | Anna Sandionigi, Alessandra De Giani, Francesco Tursi, Angela Michelotti, Enza Cestone, Silvana Giardina, Jessica Zampolli, and Patrizia Di Gennaro |
| Title | Development of a rational framework for the therapeutic efficacy of fecal microbiota transplantation for calf diarrhea treatment |
| Journal | Microbiome. 2022. |
| Authors | Islam J, Tanimizu M, Shimizu Y, Goto Y, Ohtani N, Sugiyama K, Tatezaki E, Sato M, Makino E, Shimada T, Ueda C, Matsuo A, Suyama Y, Sakai Y, Furukawa M, Usami K, Yoneyama H, Aso H, Tanaka H, Nochi T |
| Title | Human Stool Preservation Impacts Taxonomic Profiles in 16S Metagenomics Studies |
| Journal | Frontiers in Cellular and Infection Microbiology. 2020. |
| Authors | A. Plauzolles, E. Toumi, M. Bonnet, G. Pénaranda, G. Bidaut, L. Chiche, J. Allardet-Servent, F. Retornaz, B. Goutorbe, & P. Halfon |
| Title | Air pollution exposure is associated with the gut microbiome as revealed by shotgun metagenomic sequencing |
| Journal | Journal of Environmental Management. 2020. |
| Authors | Farnaz Fouladi, Maximilian J. Bailey, William B. Patterson, Michael Sioda, Ivory C. Blakley, Anthony A. Fodor, Roshonda B. Jones, Zhanghua Chen, Jeniffer S. Kim, Frederick Lurmann, Cameron Martino, Rob Knight, Frank D. Gilliland, Tanya L. Alderete |
| Title | Altered Fecal Small RNA Profiles in Colorectal Cancer Reflect Gut Microbiome Composition in Stool Samples |
| Journal | ASM Journals. 2019. |
| Authors | Sonia Tarallo, Giulio Ferrero, Gaetano Gallo, Antonio Francavilla, Giuseppe Clerico, Alberto Realis Luc, Paolo Manghi, Francesca Cordero |
| Title | Bacteroidales markers for microbial source tracking in Southeast Asia |
| Journal | Water Research. 2017. |
| Authors | Jean Pierre Nshimyimana, Mercedes C. Cruz, R. Janelle Thompson, Stefan Wuertz |
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