Streptococcus agalactiae TaqMan PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Streptococcus agalactiae TaqMan PCR Detection Kits Price USD$699.00 SKU TM30650 Format PCR Probe/Primer and Control Set Size 100 Reactions Quantity Overview Details Documentation Citations Overview Detection kits for Streptococcus agalactiae Available in TaqMan format for analysis Mastitis is the single most costly disease of dairy cattle resulting in the reduction of milk yield and quality. The inflammation of the udder is mainly caused by infection of various bacteria. One of such mastitis bacteria, Streptococcus agalactiae, is highly infectious and causes mainly subclinical infections, which are not identified by the herdsman. As a result, S. agalactiae can spread widely within a herd, causing immediate loss due to reduced milk. S. agalactiae is a gram-positive bacteria belonging to the Group B streptococci. Traditional cultural identification of S. agalactiae is based on S. agalactiae being beta-hemolytic as well as presence of group B Lancefield antigen and by its ability to hydrolyze sodium hippurate. TaqMan PCR Kit Click to expand options Streptococcus agalactiae TaqMan PCR Kit, 100 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents Streptococcus agalactiae TaqMan PCR Probe/Primer Set and Controls, 100 reactions Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest Nuclease-free water Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix For research use only and NOT intended for in vitro diagnostics. Details Supporting Data Previous TaqMan PCR Kit - Figure 1. Example of TaqMan PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 2. Example of TaqMan PCR Negative result. No target DNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR. TaqMan PCR Kit - Figure 3. Example of TaqMan PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification. Next Click for expanded view Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. Component Cat. TM30650 (100 preps) Cat. TM30610 (100 preps) MDx TaqMan 2X PCR Master Mix 2 x 700 μL - S. agalactiae Primer & Probe Mix 280 μL 280 μL S. agalactiae Positive Control 150 μL 150 μL Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL Product Insert 1 1 Documentation PROTOCOLS (TM30650) Streptococcus agalactiae TaqMan PCR Kit - Protocol (100 rxns) (TM30610) Streptococcus agalactiae TaqMan Probe/Primer and Control Set - Protocol (100 rxns) Related Products Streptococcus agalactiae TaqMan Probe/Primer and Control Set Milk Bacterial DNA Isolation Kit
Streptococcus agalactiae TaqMan PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Streptococcus agalactiae TaqMan PCR Detection Kits Price USD$699.00 SKU TM30650 Format PCR Probe/Primer and Control Set Size 100 Reactions Quantity