Streptococcus dysgalactiae TaqMan PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Streptococcus dysgalactiae TaqMan PCR Detection Kits Price USD$699.00 SKU TM30750 Format PCR Probe/Primer and Control Set Size 100 Reactions Quantity Overview Details Documentation Citations Overview Detection kits for Streptococcus dysgalactiae Available in TaqMan format for analysis Mastitis is the single most costly disease of dairy cattle resulting in the reduction of milk yield and quality. The inflammation of the utter is mainly caused by infection of various bacteria. Streptococcus dysgalactiae, a Lancefield group C α-hemolytic gram-positive bacteria, is a common pathogen in subclinical and clinical mastitis causing substantial economic losses in dairy herds. Streptococcus dysgalactiae has been isolated from infected mammary glands and teat injuries and is transmitted primarily during milking. However, detection of S. dysgalactiae in other cow tissues such as tonsils, mouth and vagina, and occurrence of S. dysgalactiae mastitis during the non-lactating period in herds, suggests that the organism also behaves as an environmental pathogen. TaqMan PCR Kit Click to expand options Streptococcus dysgalactiae TaqMan PCR Kit, 100 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents Streptococcus dysgalactiae TaqMan PCR Probe/Primer Set and Controls, 100 reactions Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest Nuclease-free water Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix For research use only and NOT intended for in vitro diagnostics. Details Supporting Data Previous TaqMan PCR Kit - Figure 1. Example of TaqMan PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 2. Example of TaqMan PCR Negative result. No target DNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR. TaqMan PCR Kit - Figure 3. Example of TaqMan PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification. Next Click for expanded view Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. Component Cat. TM30750 (100 preps) Cat. TM30710 (100 preps) MDx TaqMan 2X PCR Master Mix 2 x 700 μL - S. dysgalactiae Primer & Probe Mix 180 μL 280 μL S. dysgalactiae Positive Control 150 μL 150 μL Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL Product Insert 1 1 Documentation PROTOCOLS (TM30750) Streptococcus dysgalactiae TaqMan PCR Kit - Protocol (100 rxns) Citations Powered by Bioz See more details on Bioz Related Products Streptococcus dysgalactiae TaqMan Probe/Primer and Control Set Milk Bacterial DNA Isolation Kit
Streptococcus dysgalactiae TaqMan PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Streptococcus dysgalactiae TaqMan PCR Detection Kits Price USD$699.00 SKU TM30750 Format PCR Probe/Primer and Control Set Size 100 Reactions Quantity
