• Ready-to-use
  • Quantitative
  • Highly Stable
  • Precise
  • Fifteen discrete fragments ranging from 300bp to 24000bp
  • Higher intensity reference bands at 5000bp and 10000bp.

Items Cat. # Size
UltraRanger 1kb DNA Ladder 12100 100 loads

UltraRanger 1 kb DNA Ladder

The Norgen UltraRanger 1 kb DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our UltraRanger contains fifteen discrete fragments ranging from 300bp to 24000bp with higher intensity reference bands at 5000bp and 10000bp. This ladder is ideal for sizing digested DNA of high molecular weight.

Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

  • Ready-to-use
  • Quantitative
  • Highly Stable
  • Precise

UltraRanger 1kb DNA Ladder (Cat# 12100) - 100 loads

Ladder Properties:
• Fifteen discrete fragments ranging from 300bp to 24000bp
• Higher intensity reference bands at 5000bp and 10000bp.

 

Fragment
Size (bp)
Mass (ng)
1
24000
30
2
14000
27
3
10000
60
4
8000
44
5
6000
36
6
5000
55
7
4000
49
8
3000
39
9
2500
35
10
2000
30
11
1500
33
12 1000

10

13 700

21

14 500

18

15 300

11

Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
 
Storage: 
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
 

 

Title The genome and proteome of Serratia bacteriophage eta which forms unstable lysogens.
Journal Virology Journal. 2014.
Authors Denyes JM, Krell PJ, Manderville RA, Ackermann HW, She YM, Kropinski AM.
Title Red/ET recombination with chimeric oligonucleotides allows rapid generation of BAC transgenes harboring full-length or truncated huntingtin cDNA.
Journal Biotechniques. 2012.
Authors Hager S, L?sch S, Noll S, Khan-Vaughan L, Ehrlich M, Kranz H.
Title Unique Changes in Mitochondrial Genomes Associated with Reversions of S-Type Cytoplasmic Male Sterility in Maizemar
Journal PLoS ONE. 2011.
Authors Matera J, Monroe J, Smelser W, Gabay-Laughnan S, Newton K.