UltraRanger 1 kb DNA Ladder (Cat. 12100)
UltraRanger 1 kb DNA Ladder
Cat. 12100
![Norgen Biotek DNA Ladders](https://norgenbiotek.com/sites/default/files/styles/original/public/ladders_9.jpg?itok=KXH9cRxU" "MidRanger 1 kb DNA Ladder")
- Professional Tech Support with Senior R&D Scientist
- Fast and Flexible Ordering Options (online, email, or phone)
- Volume Discounts Available on Large Quantity Orders*
*Inquire by phone or email for more information.
UltraRanger 1 kb DNA Ladder
Cat. 12100
Ideal for sizing digested DNA of high molecular weight
- Ready-to-use
- Quantitative
- Highly Stable
- Precise
- Fifteen discrete fragments ranging from 300Â bp to 24000Â bp
- Higher intensity reference bands at 5000Â bp and 10000Â bp
For research use only and NOT intended for in vitro diagnostics.
Want a discount?
Create your account to receive 15% off on your first purchase!
UltraRanger 1 kb DNA Ladder
(Cat. 12100) 100 loads
(Cat. 12100) 100 loads
Product Size
Sign in to see your price
Price is dependent on a variety of factors including location and account type. Please sign in to your account to view more accurate pricing.
Kit Specifications
You have selected: Cat. 12100
UltraRanger 1kb DNA Ladder (Cat# 12100) - 100 loads
Ladder Properties:
• Fifteen discrete fragments ranging from 300 bp to 24000 bp
• Fifteen discrete fragments ranging from 300 bp to 24000 bp
• Higher intensity reference bands at 5000 bp and 10000 bp
|
Fragment
|
Size (bp)
|
Mass (ng)
|
|
1
|
24000
|
30
|
|
2
|
14000
|
27
|
|
3
|
10000
|
60
|
|
4
|
8000
|
44
|
|
5
|
6000
|
36
|
|
6
|
5000
|
55
|
|
7
|
4000
|
49
|
|
8
|
3000
|
39
|
|
9
|
2500
|
35
|
|
10
|
2000
|
30
|
|
11
|
1500
|
33
|
| 12 | 1000 |
10 |
| 13 | 700 |
21 |
| 14 | 500 |
18 |
| 15 | 300 |
11 |
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
Supporting Data
Click for expanded view
Protocols:
Application Notes:
Safety Data Sheets:
| Title | The genome and proteome of Serratia bacteriophage eta which forms unstable lysogens. |
| Journal | Virology Journal. 2014. |
| Authors | Denyes JM, Krell PJ, Manderville RA, Ackermann HW, She YM, Kropinski AM. |
| Title | Red/ET recombination with chimeric oligonucleotides allows rapid generation of BAC transgenes harboring full-length or truncated huntingtin cDNA. |
| Journal | Biotechniques. 2012. |
| Authors | Hager S, L?sch S, Noll S, Khan-Vaughan L, Ehrlich M, Kranz H. |
| Title | Differences in the quantity of DNA found in the urine and saliva of smokers versus nonsmokers: implications for the timing of epigenetic events. |
| Journal | Epigenomics. 2012. |
| Authors | Simkin M, Abdalla M, El-Mogy M, Haj-Ahmad Y. |
| Title | Variation of Serine-Aspartate Repeats in Membrane Proteins Possibly Contributes to Staphylococcal Microevolution. |
| Journal | PLOS One. 2011. |
| Authors | Cheng J, Xue H, Zhao X. |
| Title | Unique Changes in Mitochondrial Genomes Associated with Reversions of S-Type Cytoplasmic Male Sterility in Maizemar |
| Journal | PLoS ONE. 2011. |
| Authors | Matera J, Monroe J, Smelser W, Gabay-Laughnan S, Newton K. |
UltraRanger 1 kb DNA Ladder
The Norgen UltraRanger 1 kb DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our UltraRanger contains fifteen discrete fragments ranging from 300Â bp to 24000Â bp with higher intensity reference bands at 5000Â bp and 10000Â bp. This ladder is ideal for sizing digested DNA of high molecular weight.
Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
Supporting Data
Click for expanded view
Kit Specifications
You have selected: Cat. 12100
UltraRanger 1kb DNA Ladder (Cat# 12100) - 100 loads
Ladder Properties:
• Fifteen discrete fragments ranging from 300 bp to 24000 bp
• Fifteen discrete fragments ranging from 300 bp to 24000 bp
• Higher intensity reference bands at 5000 bp and 10000 bp
|
Fragment
|
Size (bp)
|
Mass (ng)
|
|
1
|
24000
|
30
|
|
2
|
14000
|
27
|
|
3
|
10000
|
60
|
|
4
|
8000
|
44
|
|
5
|
6000
|
36
|
|
6
|
5000
|
55
|
|
7
|
4000
|
49
|
|
8
|
3000
|
39
|
|
9
|
2500
|
35
|
|
10
|
2000
|
30
|
|
11
|
1500
|
33
|
| 12 | 1000 |
10 |
| 13 | 700 |
21 |
| 14 | 500 |
18 |
| 15 | 300 |
11 |
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
Protocols:
Application Notes:
Safety Data Sheets:
| Title | The genome and proteome of Serratia bacteriophage eta which forms unstable lysogens. |
| Journal | Virology Journal. 2014. |
| Authors | Denyes JM, Krell PJ, Manderville RA, Ackermann HW, She YM, Kropinski AM. |
| Title | Red/ET recombination with chimeric oligonucleotides allows rapid generation of BAC transgenes harboring full-length or truncated huntingtin cDNA. |
| Journal | Biotechniques. 2012. |
| Authors | Hager S, L?sch S, Noll S, Khan-Vaughan L, Ehrlich M, Kranz H. |
| Title | Differences in the quantity of DNA found in the urine and saliva of smokers versus nonsmokers: implications for the timing of epigenetic events. |
| Journal | Epigenomics. 2012. |
| Authors | Simkin M, Abdalla M, El-Mogy M, Haj-Ahmad Y. |
| Title | Variation of Serine-Aspartate Repeats in Membrane Proteins Possibly Contributes to Staphylococcal Microevolution. |
| Journal | PLOS One. 2011. |
| Authors | Cheng J, Xue H, Zhao X. |
| Title | Unique Changes in Mitochondrial Genomes Associated with Reversions of S-Type Cytoplasmic Male Sterility in Maizemar |
| Journal | PLoS ONE. 2011. |
| Authors | Matera J, Monroe J, Smelser W, Gabay-Laughnan S, Newton K. |