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For the rapid and efficient purification of DNA from exfoliated cells or bacteria in urine
For research use only and NOT intended for in vitro diagnostics.
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Kit Specifications
Kit Specifications
|
|
Minimum Urine Input
|
1 mL
|
Maximum Urine Input
|
50 mL
|
Time to Complete 10 Purifications
|
10 minutes
(Plus a 30 minute incubation - Bacteria) (Plus a 15 minute incubation - Exfoliated) |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.
Supporting Data
Title | Disinfection of polymicrobial urines by electrochemical oxidation: Removal of antibiotic-resistant bacteria and genes |
Journal | Journal of Hazardous Materials, Volume 426, 2022. |
Authors | Miguel Herraiz-Carboné, Salvador Cotillas, Engracia Lacasa, Marina Vasileva, Caridad Sainz de Baranda, Eva Riquelme, Pablo Cañizares, Cristina Sáez |
Title | Investigating the association between the urinary microbiome and bladder cancer: An exploratory study. |
Journal | Urologic Oncology: Seminars and Original Investigations. Volume 39, Issue 6, 2021. |
Authors | Hussein A, Elsayed A, Durrani M, et al. |
Title | Emerging Tuberculosis Pathogen Hijacks Social Communication Behavior in the Group-Living Banded Mongoose (Mungos mungo) |
Journal | mBio. 2016. |
Authors | Alexander KA, Sanderson CE, Larsen MH, Robbe-Austerman S, Williams MC, Palmer MV |
Title | Performance of the HSV OligoGen kit for the diagnosis of herpes simplex virus type 1 and 2 |
Journal | Diagnostic Microbiology and Infectious Disease. 2016. |
Authors | Manuel Parra-Sáncheza, Ana Marcuello Lópezb, Silvia GarcÃa-Reya, Ismail Zakariya-Yousef Brevala, Samuel Bernal MartÃneza, Isabel Pueyo RodrÃguezc, Estrella MartÃn-Mazuelosa, José Carlos Palomares FolÃaa |
Title | Genomic complexity of urothelial bladder cancer revealed in urinary cfDNA |
Journal | European Journal of Human Genetics. 2016. |
Authors | Togneri FS, Ward DG, Foster JM, Devall AJ, Wojtowicz P, Alyas S, Vasques FR, Oumie A, James ND, Cheng KK, Zeegers MP, Deshmukh N, O'Sullivan B, Taniere P, Spink KG, McMullan DJ, Griffiths M1, Bryan RT |
Title | The Role of Urinary Cathelicidin (LL-37) and Human β-defensin 1 (hBD-1) in Uncomplicated Escherichia coli Urinary Tract Infections. |
Journal | Infection and Immunity. 2014. |
Authors | Nielsen KL, Dynesen P, Larsen P, Jakobsen L, Andersen PS, Frimodt-Møller N. |
Urine DNA Isolation Kit for Exfoliated Cells or Bacteria
Norgen’s Urine DNA Isolation Kit for Exfoliated Cells or Bacteria is designed for the rapid isolation of either: 1) human genomic DNA from exfoliated cells that have been shed into the urine from the urinary tract; or 2) bacterial genomic DNA from urine samples. The kit allows for the isolation of DNA from 1 to 50 mL of urine. The genomic DNA isolated from exfoliated cells can be used in a number of diagnostic and research applications including the diagnosis and monitoring of bladder, kidney, or other urinary-tract cancers. Bacterial genomic DNA from both human urine samples and urine samples from animals can be isolated with this kit in order to study the levels and types of bacteria that are present. The kit allows for the isolation of genomic DNA from both Gram negative and Gram positive bacteria, including E. coli, Proteus spp., Klebsiella spp., Enterobacter spp., Serratia spp., Pseudomonas spp, Clostridial ssp. and Leptospirosis spp.Chlamydia trachomatis and Neisseria gonorrhoeae.
Typical yields of human genomic DNA from exfoliated cells will vary depending on the cell density of the urine sample, which is affected by a number of factors including health, diet and sex of the individual donating the urine. Typical yields of bacterial genomic DNA will vary depending on the urine sample and the bacterial species, if any, present in the urine. Healthy humans generally have < 10, 000 CFU of bacteria per mL of urine, and this kit is sufficiently sensitive to isolate and detect DNA from even this minimal amount of bacteria. The genomic DNA purified with this kit is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications such as PCR, qPCR and Southern Blot analysis.
Supporting Data
Kit Specifications
Kit Specifications
|
|
Minimum Urine Input
|
1 mL
|
Maximum Urine Input
|
50 mL
|
Time to Complete 10 Purifications
|
10 minutes
(Plus a 30 minute incubation - Bacteria) (Plus a 15 minute incubation - Exfoliated) |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.
Title | Disinfection of polymicrobial urines by electrochemical oxidation: Removal of antibiotic-resistant bacteria and genes |
Journal | Journal of Hazardous Materials, Volume 426, 2022. |
Authors | Miguel Herraiz-Carboné, Salvador Cotillas, Engracia Lacasa, Marina Vasileva, Caridad Sainz de Baranda, Eva Riquelme, Pablo Cañizares, Cristina Sáez |
Title | Investigating the association between the urinary microbiome and bladder cancer: An exploratory study. |
Journal | Urologic Oncology: Seminars and Original Investigations. Volume 39, Issue 6, 2021. |
Authors | Hussein A, Elsayed A, Durrani M, et al. |
Title | Emerging Tuberculosis Pathogen Hijacks Social Communication Behavior in the Group-Living Banded Mongoose (Mungos mungo) |
Journal | mBio. 2016. |
Authors | Alexander KA, Sanderson CE, Larsen MH, Robbe-Austerman S, Williams MC, Palmer MV |
Title | Performance of the HSV OligoGen kit for the diagnosis of herpes simplex virus type 1 and 2 |
Journal | Diagnostic Microbiology and Infectious Disease. 2016. |
Authors | Manuel Parra-Sáncheza, Ana Marcuello Lópezb, Silvia GarcÃa-Reya, Ismail Zakariya-Yousef Brevala, Samuel Bernal MartÃneza, Isabel Pueyo RodrÃguezc, Estrella MartÃn-Mazuelosa, José Carlos Palomares FolÃaa |
Title | Genomic complexity of urothelial bladder cancer revealed in urinary cfDNA |
Journal | European Journal of Human Genetics. 2016. |
Authors | Togneri FS, Ward DG, Foster JM, Devall AJ, Wojtowicz P, Alyas S, Vasques FR, Oumie A, James ND, Cheng KK, Zeegers MP, Deshmukh N, O'Sullivan B, Taniere P, Spink KG, McMullan DJ, Griffiths M1, Bryan RT |
Title | The Role of Urinary Cathelicidin (LL-37) and Human β-defensin 1 (hBD-1) in Uncomplicated Escherichia coli Urinary Tract Infections. |
Journal | Infection and Immunity. 2014. |
Authors | Nielsen KL, Dynesen P, Larsen P, Jakobsen L, Andersen PS, Frimodt-Møller N. |