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Urine DNA Isolation Kit for Exfoliated Cells or Bacteria

Cat. 47050
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Urine DNA Isolation Kit for Exfoliated Cells or Bacteria

Cat. 47050

For the rapid and efficient purification of DNA from exfoliated cells or bacteria in urine

  • Isolate genomic DNA from either exfoliated cells or bacteria found in urine
  • Isolate and detect genomic DNA from as little as 1 mL of urine and up to 50 mL urine
  • High quality DNA for sensitive applications
  • Rapid processing time

For research use only and NOT intended for in vitro diagnostics.

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Urine DNA Isolation Kit for Exfoliated Cells or Bacteria
(Cat. 47050)
50 preps

Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

Kit Specifications

You have selected: Cat. 47050
Kit Specifications
Minimum Urine Input
1 mL
Maximum Urine Input
50 mL
Time to Complete 10 Purifications
10 minutes
(Plus a 30 minute incubation - Bacteria)
(Plus a 15 minute incubation - Exfoliated)

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.

Supporting Data

Click for expanded view

Title Disinfection of polymicrobial urines by electrochemical oxidation: Removal of antibiotic-resistant bacteria and genes
Journal Journal of Hazardous Materials, Volume 426, 2022.
Authors Miguel Herraiz-Carboné, Salvador Cotillas, Engracia Lacasa, Marina Vasileva, Caridad Sainz de Baranda, Eva Riquelme, Pablo Cañizares, Cristina Sáez
Title Investigating the association between the urinary microbiome and bladder cancer: An exploratory study.
Journal Urologic Oncology: Seminars and Original Investigations. Volume 39, Issue 6, 2021.
Authors Hussein A, Elsayed A, Durrani M, et al.
Title Emerging Tuberculosis Pathogen Hijacks Social Communication Behavior in the Group-Living Banded Mongoose (Mungos mungo)
Journal mBio. 2016.
Authors Alexander KA, Sanderson CE, Larsen MH, Robbe-Austerman S, Williams MC, Palmer MV
Title Performance of the HSV OligoGen kit for the diagnosis of herpes simplex virus type 1 and 2
Journal Diagnostic Microbiology and Infectious Disease. 2016.
Authors Manuel Parra-Sáncheza, Ana Marcuello Lópezb, Silvia García-Reya, Ismail Zakariya-Yousef Brevala, Samuel Bernal Martíneza, Isabel Pueyo Rodríguezc, Estrella Martín-Mazuelosa, José Carlos Palomares Folíaa
Title Genomic complexity of urothelial bladder cancer revealed in urinary cfDNA
Journal European Journal of Human Genetics. 2016.
Authors Togneri FS, Ward DG, Foster JM, Devall AJ, Wojtowicz P, Alyas S, Vasques FR, Oumie A, James ND, Cheng KK, Zeegers MP, Deshmukh N, O'Sullivan B, Taniere P, Spink KG, McMullan DJ, Griffiths M1, Bryan RT
Title The Role of Urinary Cathelicidin (LL-37) and Human β-defensin 1 (hBD-1) in Uncomplicated Escherichia coli Urinary Tract Infections.
Journal Infection and Immunity. 2014.
Authors Nielsen KL, Dynesen P, Larsen P, Jakobsen L, Andersen PS, Frimodt-Møller N.

Urine DNA Isolation Kit for Exfoliated Cells or Bacteria

Norgen’s Urine DNA Isolation Kit for Exfoliated Cells or Bacteria is designed for the rapid isolation of either: 1) human genomic DNA from exfoliated cells that have been shed into the urine from the urinary tract; or 2) bacterial genomic DNA from urine samples. The kit allows for the isolation of DNA from 1 to 50 mL of urine. The genomic DNA isolated from exfoliated cells can be used in a number of diagnostic and research applications including the diagnosis and monitoring of bladder, kidney, or other urinary-tract cancers. Bacterial genomic DNA from both human urine samples and urine samples from animals can be isolated with this kit in order to study the levels and types of bacteria that are present. The kit allows for the isolation of genomic DNA from both Gram negative and Gram positive bacteria, including E. coli, Proteus spp., Klebsiella spp., Enterobacter spp., Serratia spp., Pseudomonas spp, Clostridial ssp. and Leptospirosis spp.Chlamydia trachomatis and Neisseria gonorrhoeae.

Typical yields of human genomic DNA from exfoliated cells will vary depending on the cell density of the urine sample, which is affected by a number of factors including health, diet and sex of the individual donating the urine. Typical yields of bacterial genomic DNA will vary depending on the urine sample and the bacterial species, if any, present in the urine. Healthy humans generally have < 10, 000 CFU of bacteria per mL of urine, and this kit is sufficiently sensitive to isolate and detect DNA from even this minimal amount of bacteria. The genomic DNA purified with this kit is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications such as PCR, qPCR and Southern Blot analysis.

 

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 47050
Kit Specifications
Minimum Urine Input
1 mL
Maximum Urine Input
50 mL
Time to Complete 10 Purifications
10 minutes
(Plus a 30 minute incubation - Bacteria)
(Plus a 15 minute incubation - Exfoliated)

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.

Title Disinfection of polymicrobial urines by electrochemical oxidation: Removal of antibiotic-resistant bacteria and genes
Journal Journal of Hazardous Materials, Volume 426, 2022.
Authors Miguel Herraiz-Carboné, Salvador Cotillas, Engracia Lacasa, Marina Vasileva, Caridad Sainz de Baranda, Eva Riquelme, Pablo Cañizares, Cristina Sáez
Title Investigating the association between the urinary microbiome and bladder cancer: An exploratory study.
Journal Urologic Oncology: Seminars and Original Investigations. Volume 39, Issue 6, 2021.
Authors Hussein A, Elsayed A, Durrani M, et al.
Title Emerging Tuberculosis Pathogen Hijacks Social Communication Behavior in the Group-Living Banded Mongoose (Mungos mungo)
Journal mBio. 2016.
Authors Alexander KA, Sanderson CE, Larsen MH, Robbe-Austerman S, Williams MC, Palmer MV
Title Performance of the HSV OligoGen kit for the diagnosis of herpes simplex virus type 1 and 2
Journal Diagnostic Microbiology and Infectious Disease. 2016.
Authors Manuel Parra-Sáncheza, Ana Marcuello Lópezb, Silvia García-Reya, Ismail Zakariya-Yousef Brevala, Samuel Bernal Martíneza, Isabel Pueyo Rodríguezc, Estrella Martín-Mazuelosa, José Carlos Palomares Folíaa
Title Genomic complexity of urothelial bladder cancer revealed in urinary cfDNA
Journal European Journal of Human Genetics. 2016.
Authors Togneri FS, Ward DG, Foster JM, Devall AJ, Wojtowicz P, Alyas S, Vasques FR, Oumie A, James ND, Cheng KK, Zeegers MP, Deshmukh N, O'Sullivan B, Taniere P, Spink KG, McMullan DJ, Griffiths M1, Bryan RT
Title The Role of Urinary Cathelicidin (LL-37) and Human β-defensin 1 (hBD-1) in Uncomplicated Escherichia coli Urinary Tract Infections.
Journal Infection and Immunity. 2014.
Authors Nielsen KL, Dynesen P, Larsen P, Jakobsen L, Andersen PS, Frimodt-Møller N.

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