Urine DNA Isolation Kits

Cat. 18100, 48800, 50100
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Urine DNA Isolation Kits

Cat. 18100, 48800, 50100

Fast and reliable purification of genomic and apoptotic DNA from urine

  • Rapid isolation of both small and large species of DNA from urine
  • Convenient spin column format
  • Effective removal of PCR inhibitors
  • Purified DNA is highly suited to sensitive downstream applications
  • Allows for the purification of viral DNA from urine

#TogetherWithNorgen

  • For every purchase of Urine kits during November, 10% of the proceeds will be donated to charity by Norgen.
  • Take a selfie wearing a Norgen moustache and share it using the hashtag #TogetherWithNorgen, and we will donate $10 in your name to charity.
  • Learn More by clicking here.

For research use only and NOT intended for in vitro diagnostics.

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Urine DNA Isolation Kit
(Cat. 18100)
50 preps
Urine DNA Isolation Kit (Slurry Format)
(Cat. 48800)
50 Preps
Urine DNA Isolation Maxi Kit (Slurry Format)
(Cat. 50100)
50 preps

Kit Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Kit Size

  • Rapid isolation of both small and large species of DNA from urine
  • Convenient spin column format
  • Effective removal of PCR inhibitors
  • Purified DNA is highly suited to sensitive downstream applications
  • Allows for the purification of viral DNA from urine

#TogetherWithNorgen

  • For every purchase of Urine kits during November, 10% of the proceeds will be donated to charity by Norgen.
  • Take a selfie wearing a Norgen moustache and share it using the hashtag #TogetherWithNorgen, and we will donate $10 in your name to charity.
  • Learn More by clicking here.

Urine DNA Isolation Kits

Both high molecular weight DNA (greater than 1 kb in size; mostly cell associated) and the smaller DNA (150 - 250 bp; derived from the circulation) is effectively isolated and purified using a rapid and convenient spin column protocol. This kit can be used to isolate DNA from a broad range of viruses in urine as well. Salts, metabolic wastes, proteins and other contaminants are removed to yield inhibitor-free DNA for use in sensitive applications. The DNA is of excellent quality for various downstream applications such as PCR, qPCR and DNA fingerprinting, methylation studies and more.

This kit is fully compatible with Norgen's Urine Collection and Preservation Tubes.

Urine DNA Isolation Kit

This kit provides a fast, reliable and simple procedure for isolating DNA from urine volumes ranging from 50 μL to 1.75 mL of urine. Multiple samples can be processed in 30 minutes.

Urine DNA Isolation Kit (Slurry Format)

This kit provides a fast, reliable and simple procedure for isolating DNA from urine volumes ranging from 3 mL to 25 mL. Multiple samples can be processed in 30 minutes. Multiple samples can be processed in 45 minutes.

Urine DNA Isolation Maxi Kit (Slurry Format)

This kit provides a fast, reliable and simple procedure for isolating DNA from urine volumes ranging from 25 mL of urine up to 80 mL. Multiple samples can be processed in 45 minutes.

Background

DNA found in urine can be divided into 2 basic categories. The larger species, genomic-DNA (gDNA), is generally greater than 1 kb in size, and appears to be derived mainly from exfoliated cells. The second species is smaller, generally between 150 and 250 bp (apoptotic-DNA), and derives, at least in part, from the circulation. The second species is also considered as an RNA/DNA hybrid as reported by Halicka et al. (2000). Both types of DNA can be isolated reliably using this kit.

Kit Specifications

You have selected: Cat. 18100
Kit Specifications
Volume of Urine Processed
1.75 mL
Average Yield*
Up to 50 ng
Size of DNA Purified
Large (> 1 kb)
and small (150-250 bp)
Time to Complete 10 Purifications
30 minutes hands-on time
(plus a 1 hour incubation)

* Yield will vary depending on the type of sample processed


Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.

You have selected: Cat. 48800
Kit Specifications - Spin Column
Minimum Urine Input 3 mL
Maximum Urine Input 25 mL
Time to Complete 10 Purifications < 30 minutes
Size of Urine DNA Purified Large (> 1 kb) and
small (150-250 bp)

 

Kit Specifications - 96-well
Urine Input Volume
3 - 25 mL
Elution Volume
1st Elution 100 μL
2nd Elution 100 μL
Size of DNA Purified
Large (> 1 kb) and
small (150-250 bp)
Time to Complete 96 Purifications
< 1 hour

 

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm up Slurry B1 and Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed. Slurry B1 contains a grey resin that will not dissolve when warmed.

You have selected: Cat. 50100
Kit Specifications
Minimum Urine Input 25 mL
Maximum Urine Input 80 mL
Time to Complete Purification < 45 minutes
Size of Urine DNA Purified Large (> 1 kb) and small (150-250 bp)

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm up Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Supporting Data

Click for expanded view

Component Cat. 18100 (50 preps) Cat. 48800 (50 preps) Cat. 50100 (50 preps)
Binding Solution K 15 mL - -
Slurry B1 - 18 mL 18 mL
Proteinase K in Storage Buffer 2 mL - -
Pronase K in Storage Buffer 2 mL - -
Soluton WN 9 mL - -
Binding Buffer A - - 50 mL
Lysis Buffer A - 30 mL 30 mL
Wash Solution A - 38 mL 38 mL
Wash Solution B 30 mL - -
Wash Solution D 9 mL - -
Binding Solution K 15 mL - -
Elution Buffer B 15 mL 15 mL 15 mL
Micro Spin Columns 50 - -
Mini Filter Spin Columns - 50 50
Collection Tubes 50 50 50
Elution Tubes (1.7 mL) 100 100 100
Product Insert 1 1 1

Regular (18100)

Title Depletion of ARGs in antibiotic-resistance Klebsiella, Pseudomonas and Staphylococcus in hospital urines by electro and photo-electro disinfection
Journal Journal of Water Process Engineering. 2022.
Authors Miguel Herraiz-Carboné, Salvador Cotillas, Engracia Lacasa, Pablo Cañizares, Manuel A. Rodrigo, Cristina Sáez
Title Stool is a sensitive and noninvasive source of DNA for monitoring expansion in repeat expansion disease mouse models
Journal Dis Model Mech. 2022.
Authors Xiaonan Zhao, Cassandra McHugh, Sydney R. Coffey, Diego Antonio Jimenez, Elizabeth Adams, Jeffrey B. Carroll, Karen Usdin
Title Clinical features of chronic kidney disease in dogs with the serological presence of Leptospira spp, Ehrlichia canis and Anaplasma phagocytophilum
Journal Research Square. 2022.
Authors Claire A. Vázquez-Manzanilla, María F. Cárdenas-Marrufo, Eduardo Gutiérrez-Blanco, Matilde Jiménez-Coello, Nelly R. Pech-Sosa, Antonio Ortega-Pacheco
Title IgA nephropathy is associated with elevated urinary mitochondrial DNA copy numbers.
Journal Scientific Reports, 9(1), 1–9. 2019.
Authors Yu, B. C., Cho, N.-J., Park, S., Kim, H., Choi, S. J., Kim, J. K., Hwang, S. D., Gil, H.-W., Lee, E. Y., Jeon, J. S., Noh, H., Han, D. C., Kim, Y. H., Jin, S.-Y., Park, M. Y., & Kwon, S. H.
Title Urine cell-free DNA is a biomarker for nephroblastomatosis or Wilms tumor in PIK3CA-related overgrowth spectrum (PROS).
Journal Genetics in Medicine. 2018.
Authors Waberski, M. B., Lindhurst, M., Keppler-Noreuil, K. M., Sapp, J. C., Baker, L., Gripp, K. W., ... & Biesecker, L. G.
Title Glomerular Hyperfiltration in Obese African American Hypertensive Patients Is Associated With Elevated Urinary Mitochondrial-DNA Copy Number.
Journal American Journal of Hypertension. 2017.
Authors Eirin, A., Saad, A., Woollard, J. R., Juncos, L. A., Calhoun, D. A., Tang, H., ... & Lerman, L. O.
Title Emerging Tuberculosis Pathogen Hijacks Social Communication Behavior in the Group-Living Banded Mongoose (Mungos mungo)
Journal ASM Journals. 2016.
Authors Kathleen A. Alexander, Claire E. Sanderson, Michelle H. Larsen, Suelee Robbe-Austerman, Mark C. Williams, Mitchell V.
Title The potential use of urine cell free DNA as a marker for cancer
Journal Expert Review of Molecular Diagnostics. 2016.
Authors Salvi S, Martignano F, Molinari C, Gurioli G, Calistri D, De Giorgi U, Conteduca V, Casadio V.
Title Urinary Mitochondrial DNA Copy Number Identifies Chronic Renal Injury in Hypertensive Patients
Journal Hypertension. 2016.
Authors Eirin A, Saad A, Tang H, Herrmann SM, Woollard JR, Lerman A, Textor SC, Lerman LO
Title Multigene Methylation Analysis And The Noninvasive Diagnosis Of Prostate Cancer From Body Fluids
Journal European Scientific Journal. 2016.
Authors Raluca, D., Anca, T., Ionescu, D., Florin, R. A., & Razvan, B
Title Epigenetic silencing of the MUPCDH gene as a possible prognostic biomarker for cyst growth in ADPKD
Journal Scientific Reports. 2015.
Authors Yu Mi Woo, Yubin Shin, Jung-Ah Hwang, Young-Hwan Hwang, Sunyoung Lee, Eun Young Park, Hyun Kyung Kong, Hayne Cho Park, Yeon-Su Lee & Jong Hoon Park
Title Long-Term Frozen Storage of Urine Samples: A Trouble to Get PCR Results in Schistosoma spp. DNA Detection?
Journal PLOS ONE. 2013.
Authors Pedro Fernández-Soto, Virginia Velasco Tirado, Cristina Carranza Rodríguez, José Luis Pérez-Arellano, Antonio Muro
Title RIG-I detects infection with live Listeria by sensing secreted bacterial nucleic acids
Journal The EMBO Journal. 2012.
Authors Abdullah Z, Schlee M, Roth S, Mraheil MA, Barchet W, B?ttcher J, Hain T, Geiger S, Hayakawa Y, Fritz JH, Civril F, Hopfner KP, Kurts C, Ruland J, Hartmann G, Chakraborty T, Knolle PA.
Title Methods for Separate Isolation of Cell-Free DNA and Cellular DNA from Urine-Application of Methylation-Specific PCR on both DNA Fractions
Journal The Open Biomarkers Journal. 2011.
Authors Beermann A, Ghanjati F, Hermanns T, Poyet C, Pereira J, Fischer J, Wernet P and Santourlidis S.
Title Renal kallikrein excretion and epigenetics in human acute kidney injury: Expression, mechanisms and consequences
Journal BMC Nephrology.. 2011
Authors Kang SW, Shih PA, Mathew RO, Mahata M, Biswas N, Rao F, Yan L, Bouchard J, Malhotra R, Tolwani A, Khandrika S, Mehta RL, O'Connor DT.

Regular (48800)

Title Characterization of the urogenital microbiome in Miniature Schnauzers with and without calcium oxalate urolithiasis
Journal Journal of Veterinary Internal Medicine. 2022.
Authors Emily L. Coffey,Andres M. Gomez,Erin N. Burton,Jennifer L. Granick,Jody P. Lulich,Eva Furrow

Urine DNA Isolation Kits

Both high molecular weight DNA (greater than 1 kb in size; mostly cell associated) and the smaller DNA (150 - 250 bp; derived from the circulation) is effectively isolated and purified using a rapid and convenient spin column protocol. This kit can be used to isolate DNA from a broad range of viruses in urine as well. Salts, metabolic wastes, proteins and other contaminants are removed to yield inhibitor-free DNA for use in sensitive applications. The DNA is of excellent quality for various downstream applications such as PCR, qPCR and DNA fingerprinting, methylation studies and more.

This kit is fully compatible with Norgen's Urine Collection and Preservation Tubes.

Urine DNA Isolation Kit

This kit provides a fast, reliable and simple procedure for isolating DNA from urine volumes ranging from 50 μL to 1.75 mL of urine. Multiple samples can be processed in 30 minutes.

Urine DNA Isolation Kit (Slurry Format)

This kit provides a fast, reliable and simple procedure for isolating DNA from urine volumes ranging from 3 mL to 25 mL. Multiple samples can be processed in 30 minutes. Multiple samples can be processed in 45 minutes.

Urine DNA Isolation Maxi Kit (Slurry Format)

This kit provides a fast, reliable and simple procedure for isolating DNA from urine volumes ranging from 25 mL of urine up to 80 mL. Multiple samples can be processed in 45 minutes.

Background

DNA found in urine can be divided into 2 basic categories. The larger species, genomic-DNA (gDNA), is generally greater than 1 kb in size, and appears to be derived mainly from exfoliated cells. The second species is smaller, generally between 150 and 250 bp (apoptotic-DNA), and derives, at least in part, from the circulation. The second species is also considered as an RNA/DNA hybrid as reported by Halicka et al. (2000). Both types of DNA can be isolated reliably using this kit.

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 18100
Kit Specifications
Volume of Urine Processed
1.75 mL
Average Yield*
Up to 50 ng
Size of DNA Purified
Large (> 1 kb)
and small (150-250 bp)
Time to Complete 10 Purifications
30 minutes hands-on time
(plus a 1 hour incubation)

* Yield will vary depending on the type of sample processed


Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.

You have selected: Cat. 48800
Kit Specifications - Spin Column
Minimum Urine Input 3 mL
Maximum Urine Input 25 mL
Time to Complete 10 Purifications < 30 minutes
Size of Urine DNA Purified Large (> 1 kb) and
small (150-250 bp)

 

Kit Specifications - 96-well
Urine Input Volume
3 - 25 mL
Elution Volume
1st Elution 100 μL
2nd Elution 100 μL
Size of DNA Purified
Large (> 1 kb) and
small (150-250 bp)
Time to Complete 96 Purifications
< 1 hour

 

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm up Slurry B1 and Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed. Slurry B1 contains a grey resin that will not dissolve when warmed.

You have selected: Cat. 50100
Kit Specifications
Minimum Urine Input 25 mL
Maximum Urine Input 80 mL
Time to Complete Purification < 45 minutes
Size of Urine DNA Purified Large (> 1 kb) and small (150-250 bp)

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm up Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Component Cat. 18100 (50 preps) Cat. 48800 (50 preps) Cat. 50100 (50 preps)
Binding Solution K 15 mL - -
Slurry B1 - 18 mL 18 mL
Proteinase K in Storage Buffer 2 mL - -
Pronase K in Storage Buffer 2 mL - -
Soluton WN 9 mL - -
Binding Buffer A - - 50 mL
Lysis Buffer A - 30 mL 30 mL
Wash Solution A - 38 mL 38 mL
Wash Solution B 30 mL - -
Wash Solution D 9 mL - -
Binding Solution K 15 mL - -
Elution Buffer B 15 mL 15 mL 15 mL
Micro Spin Columns 50 - -
Mini Filter Spin Columns - 50 50
Collection Tubes 50 50 50
Elution Tubes (1.7 mL) 100 100 100
Product Insert 1 1 1

Regular (18100)

Title Depletion of ARGs in antibiotic-resistance Klebsiella, Pseudomonas and Staphylococcus in hospital urines by electro and photo-electro disinfection
Journal Journal of Water Process Engineering. 2022.
Authors Miguel Herraiz-Carboné, Salvador Cotillas, Engracia Lacasa, Pablo Cañizares, Manuel A. Rodrigo, Cristina Sáez
Title Stool is a sensitive and noninvasive source of DNA for monitoring expansion in repeat expansion disease mouse models
Journal Dis Model Mech. 2022.
Authors Xiaonan Zhao, Cassandra McHugh, Sydney R. Coffey, Diego Antonio Jimenez, Elizabeth Adams, Jeffrey B. Carroll, Karen Usdin
Title Clinical features of chronic kidney disease in dogs with the serological presence of Leptospira spp, Ehrlichia canis and Anaplasma phagocytophilum
Journal Research Square. 2022.
Authors Claire A. Vázquez-Manzanilla, María F. Cárdenas-Marrufo, Eduardo Gutiérrez-Blanco, Matilde Jiménez-Coello, Nelly R. Pech-Sosa, Antonio Ortega-Pacheco
Title IgA nephropathy is associated with elevated urinary mitochondrial DNA copy numbers.
Journal Scientific Reports, 9(1), 1–9. 2019.
Authors Yu, B. C., Cho, N.-J., Park, S., Kim, H., Choi, S. J., Kim, J. K., Hwang, S. D., Gil, H.-W., Lee, E. Y., Jeon, J. S., Noh, H., Han, D. C., Kim, Y. H., Jin, S.-Y., Park, M. Y., & Kwon, S. H.
Title Urine cell-free DNA is a biomarker for nephroblastomatosis or Wilms tumor in PIK3CA-related overgrowth spectrum (PROS).
Journal Genetics in Medicine. 2018.
Authors Waberski, M. B., Lindhurst, M., Keppler-Noreuil, K. M., Sapp, J. C., Baker, L., Gripp, K. W., ... & Biesecker, L. G.
Title Glomerular Hyperfiltration in Obese African American Hypertensive Patients Is Associated With Elevated Urinary Mitochondrial-DNA Copy Number.
Journal American Journal of Hypertension. 2017.
Authors Eirin, A., Saad, A., Woollard, J. R., Juncos, L. A., Calhoun, D. A., Tang, H., ... & Lerman, L. O.
Title Emerging Tuberculosis Pathogen Hijacks Social Communication Behavior in the Group-Living Banded Mongoose (Mungos mungo)
Journal ASM Journals. 2016.
Authors Kathleen A. Alexander, Claire E. Sanderson, Michelle H. Larsen, Suelee Robbe-Austerman, Mark C. Williams, Mitchell V.
Title The potential use of urine cell free DNA as a marker for cancer
Journal Expert Review of Molecular Diagnostics. 2016.
Authors Salvi S, Martignano F, Molinari C, Gurioli G, Calistri D, De Giorgi U, Conteduca V, Casadio V.
Title Urinary Mitochondrial DNA Copy Number Identifies Chronic Renal Injury in Hypertensive Patients
Journal Hypertension. 2016.
Authors Eirin A, Saad A, Tang H, Herrmann SM, Woollard JR, Lerman A, Textor SC, Lerman LO
Title Multigene Methylation Analysis And The Noninvasive Diagnosis Of Prostate Cancer From Body Fluids
Journal European Scientific Journal. 2016.
Authors Raluca, D., Anca, T., Ionescu, D., Florin, R. A., & Razvan, B
Title Epigenetic silencing of the MUPCDH gene as a possible prognostic biomarker for cyst growth in ADPKD
Journal Scientific Reports. 2015.
Authors Yu Mi Woo, Yubin Shin, Jung-Ah Hwang, Young-Hwan Hwang, Sunyoung Lee, Eun Young Park, Hyun Kyung Kong, Hayne Cho Park, Yeon-Su Lee & Jong Hoon Park
Title Long-Term Frozen Storage of Urine Samples: A Trouble to Get PCR Results in Schistosoma spp. DNA Detection?
Journal PLOS ONE. 2013.
Authors Pedro Fernández-Soto, Virginia Velasco Tirado, Cristina Carranza Rodríguez, José Luis Pérez-Arellano, Antonio Muro
Title RIG-I detects infection with live Listeria by sensing secreted bacterial nucleic acids
Journal The EMBO Journal. 2012.
Authors Abdullah Z, Schlee M, Roth S, Mraheil MA, Barchet W, B?ttcher J, Hain T, Geiger S, Hayakawa Y, Fritz JH, Civril F, Hopfner KP, Kurts C, Ruland J, Hartmann G, Chakraborty T, Knolle PA.
Title Methods for Separate Isolation of Cell-Free DNA and Cellular DNA from Urine-Application of Methylation-Specific PCR on both DNA Fractions
Journal The Open Biomarkers Journal. 2011.
Authors Beermann A, Ghanjati F, Hermanns T, Poyet C, Pereira J, Fischer J, Wernet P and Santourlidis S.
Title Renal kallikrein excretion and epigenetics in human acute kidney injury: Expression, mechanisms and consequences
Journal BMC Nephrology.. 2011
Authors Kang SW, Shih PA, Mathew RO, Mahata M, Biswas N, Rao F, Yan L, Bouchard J, Malhotra R, Tolwani A, Khandrika S, Mehta RL, O'Connor DT.

Regular (48800)

Title Characterization of the urogenital microbiome in Miniature Schnauzers with and without calcium oxalate urolithiasis
Journal Journal of Veterinary Internal Medicine. 2022.
Authors Emily L. Coffey,Andres M. Gomez,Erin N. Burton,Jennifer L. Granick,Jody P. Lulich,Eva Furrow

 


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