Fast, reliable isolation of urine DNA from 25 mL to 80 mL samples
|Urine DNA Isolation Maxi Kit (Slurry Format)||50100||50 preps|
Urine DNA Isolation Maxi Kit (Slurry Format)
This kit provides a fast, reliable and simple procedure for isolating DNA from urine volumes ranging from 25 mL of urine up to 80 mL. Both high molecular weight DNA (greater than 1 kb in size; mostly cell associated) and the smaller DNA (150 - 250 bp; derived from the circulation) is effectively isolated and purified using a rapid and convenient spin column protocol. Multiple samples can be processed in 45 minutes. Salts, metabolic wastes, proteins and other contaminants are removed to yield inhibitor-free DNA for use in sensitive applications such as PCR, qPCR, DNA fingerprinting, methylation studies and more. This kit can also be used to isolate DNA from a broad range of viruses urine.
This kit is fully compatble with Norgen's Urine Collection and Preservation Tubes.
Many advantages favour the use of urinary DNA for cancer biomarker discovery and diagnosis of different pathogens over the use of blood and tissue samples. DNA found in urine can be divided into 2 basic categories. The larger genomic DNA (gDNA) species is generally greater than 1 kb in size, and appears to be derived mainly from exfoliated cells. The second species is smaller, generally between 150 and 250 bp (apoptotic-DNA), and derives, at least in part, from the circulation. The second species is also considered as an RNA/DNA hybrid. Both types of DNA can be isolated reliably using this kit.
|Minimum Urine Input||25 mL|
|Maximum Urine Input||80 mL|
|Time to Complete Purification||< 45 minutes|
|Size of Urine DNA Purified||Large (> 1 kb) and small (150-250 bp)|
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 1 year without showing any reduction in performance. It is recommended to warm up Solution A, Solution A1 and Solution B for 20 minutes at 60°C if any salt precipitation is observed.
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