Urine Exfoliated Cell and Bacteria RNA Purification Kit For the rapid purification of total RNA (including microRNA) from exfoliated cells in urine For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Urine Exfoliated Cell and Bacteria RNA Purification Kit For the rapid purification of total RNA (including microRNA) from exfoliated cells in urine Price USD$239.00 SKU 22550 Format Spin Column Size 25 Preps Quantity Overview Details Documentation Citations Overview Purify all sizes of RNA (including microRNA) without the need for phenol Isolate and detect total RNA from 1 mL and up to 50 mL urine Provides high-quality RNA for sensitive applications - isolate RNA from as little as 100 cells Rapid processing time from sampling to downstream testing Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix This kit provides a rapid spin column method for the isolation and purification of total RNA (including microRNA) from exfoliated cells that have been shed into the urine from the urinary tract. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, as well as from the contaminating species found in urine such as glucose and salts, without the use of phenol or chloroform. The purified RNA is of the highest integrity and can be used in a number of downstream applications including real-time RT-PCR, qRT-PCR, Northern blotting, NGS, and expression array assays. Multiple samples can be processed in 20 minutes. Background RNA biomarkers from exfoliated cells can be used as non-invasive tools for a number of diagnostic and research applications including the diagnosis and monitoring of bladder, kidney, or other urinary-tract cancers. Details Supporting Data Previous Figure 1. Isolation and Detection of RNA from the Exfoliated Cells in 50 mL of Urine. RNA was isolated from the exfoliated cells in a 50 mL urine sample obtained from a healthy male without any spiking using Norgen's Urine (Exfoliated Cell) RNA Purification Kit. The bind, wash, and elute procedure was followed, and the purified RNA was recovered in 20 µL of the Elution Buffer. A dilution series of HeLa cells with known concentration (from 1 cell per mL to 1,000 cells per mL) was isolated in parallel as standards. Seven microliters of the 20 µL eluted RNA were then subjected to a 20 µL reverse transcription. One microliter of the reverse transcription was used in a 20 µL real-time PCR reaction with primers to detect the ribosomal S14 transcript. The red lines in the PCR baseline graph above correspond to the HeLa RNA standards, while the blue lines correspond to the successful PCR results when RNA isolated from the exfoliated cells in 50 mL of non-spiked urine was used as the template. Next Click for expanded view Kit Specifications Maximum Column Binding Capacity 50 μg Volume of Urine Processed 1-50 mL Maximum Input of Exfoliated Cells 1 x 106 Size of RNA Purified All sizes, including small RNA (< 200 nt) Time to Complete 10 Purifications 20 minutes (for Exfoliated Cells) 30 minutes (for Bacteria) Average Yield ~1μg RNA per 1 x 105 cells (For Exfoliated Cells - Varies due to cell density sample) ~0.5μg RNA per 1 x 107 cells (For Bacteria - Varies due to cell density sample) Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. Component Cat. 22550 (25 preps) Buffer SK 15 mL Wash Solution A 18 mL Elution Buffer E 6 mL Micro Spin Columns 25 Collection Tubes 25 Elution Tubes (1.7 mL) 25 Product Insert 1 Documentation PROTOCOLS (22550) Urine Exfoliated Cell and Bacteria RNA Purification Kit - Protocol (25 prep) SAFETY DATA SHEETS (22550) Urine Exfoliated Cell and Bacteria RNA Purification Kit - Safety Data Sheet RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class How to Reduce False Negative SARS-CoV-2 Results with Silicon Carbide Citations Title Integrated RNA and metabolite profiling of urine liquid biopsies for prostate cancer biomarker discovery. Journal Scientific Reports. Volume 10, Article: 3716. 2020. Authors Lee, B., Mahmud, I., Marchica, J. et al. Title Next Generation Sequencing of urine exfoliated cells: an approach of prostate cancer microRNAs research. Journal Scientific Reports. Volume 8, Article: 7111. 2018. Authors Guelfi, G., Cochetti, G., Stefanetti, V. et al. Title Development of a Voided Urine Assay for Detecting Prostate Cancer Noninvasively: A Pilot Study Journal BJU International. 2017. Authors Trabulsi EJ, Tripathi SK, Gomella L, Solomides C, Wickstrom E, Thakur ML Title Evaluation of urinary XIAP as a diagnostic biomarker of carcinoma of urinary bladder. Journal Tumor Biology. 2014. Authors Vaccaro M, Malafronte N, Alfieri M, Tommasi N, Leone A. Title Long Noncoding RNAs as Putative Biomarkers for Prostate Cancer Detection. Journal The Journal of Molecular Diagnostics. 2014. Authors Bongyong Lee, Joseph Mazar, Muhammad N. Aftab, Feng Qi, John Shelley, Jian-Liang Li, Subramaniam Govindarajan, Felipe Valerio, Inoel Rivera, Tadzia Thurn, Tien Anh Tran, Darian Kameh, Vipul Patel‡, Ranjan J. Perera. Title A pilot study of urinary microRNA as a biomarker for urothelial cancer. Journal Canadian Urolological Association Journal. 2013. Authors Snowdon J, Boag S, Feilotter H, Izard J, Siemens DR. Related Products RNase-Free DNase I Kit Total RNA Purification Kit Cytoplasmic & Nuclear RNA Purification Kit Leukocyte RNA Purification Kit microRNA (cel-miR-39) Spike-In Kit
Urine Exfoliated Cell and Bacteria RNA Purification Kit For the rapid purification of total RNA (including microRNA) from exfoliated cells in urine For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Urine Exfoliated Cell and Bacteria RNA Purification Kit For the rapid purification of total RNA (including microRNA) from exfoliated cells in urine Price USD$239.00 SKU 22550 Format Spin Column Size 25 Preps Quantity