Urine Exfoliated Cell and Bacteria RNA Purification Kit (Cat. 22550)

Urine Exfoliated Cell and Bacteria RNA Purification Kit

Cat. 22550

Urine Exfoliated Cell RNA Purification Kit

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Urine Exfoliated Cell and Bacteria RNA Purification Kit

Cat. 22550

For the rapid purification of total RNA (including microRNA) from exfoliated cells in urine

Purify all sizes of RNA (including microRNA) without the need for phenol
Isolate and detect total RNA from 1 mL and up to 50 mL urine
Provides high-quality RNA for sensitive applications - isolate RNA from as little as 100 cells
Rapid processing time from sampling to downstream testing
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

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Urine Exfoliated Cell RNA Purification Kit
(Cat. 22550) 25 preps

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Kit Size

Urine Exfoliated Cell and Bacteria RNA Purification Kit

This kit provides a rapid spin column method for the isolation and purification of total RNA (including microRNA) from exfoliated cells that have been shed into the urine from the urinary tract. The total RNA is preferentially purified from other cellular components such as proteins without the use of phenol or chloroform. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, as well as from the contaminating species found in urine such as glucose and salts, without the use of phenol or chloroform. The purified RNA is of the highest integrity and can be used in a number of downstream applications including real-time RT-PCR, qRT-PCR, Northern blotting, NGS, and expression array assays. Multiple samples can be processed in 20 minutes.

Background

RNA biomarkers from exfoliated cells can be used as non-invasive tools for a number of diagnostic and research applications including the diagnosis and monitoring of bladder, kidney, or other urinary-tract cancers.

Kit Specifications

You have selected: Cat. 22550

Kit Specifications
Maximum Column Binding Capacity	50 μg
Volume of Urine Processed	1-50 mL
Maximum Input of Exfoliated Cells	1 x 10⁶
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Time to Complete 10 Purifications	20 minutes (for Exfoliated Cells) 30 minutes (for Bacteria)
Average Yield	~1μg RNA per 1 x 10⁵ cells (For Exfoliated Cells - Varies due to cell density sample) ~0.5μg RNA per 1 x 10⁷ cells (For Bacteria - Varies due to cell density sample)

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Supporting Data

Figure 1. Isolation and Detection of RNA from the Exfoliated Cells in 50 mL of Urine. RNA was isolated from the exfoliated cells in a 50 mL urine sample obtained from a healthy male without any spiking using Norgen's Urine (Exfoliated Cell) RNA Purification Kit. The bind, wash, and elute procedure was followed, and the purified RNA was recovered in 20 µL of the Elution Buffer. A dilution series of HeLa cells with known concentration (from 1 cell per mL to 1,000 cells per mL) was isolated in parallel as standards. Seven microliters of the 20 µL eluted RNA were then subjected to a 20 µL reverse transcription. One microliter of the reverse transcription was used in a 20 µL real-time PCR reaction with primers to detect the ribosomal S14 transcript. The red lines in the PCR baseline graph above correspond to the HeLa RNA standards, while the blue lines correspond to the successful PCR results when RNA isolated from the exfoliated cells in 50 mL of non-spiked urine was used as the template.

Click for expanded view

Component	Cat. 22550 (25 preps)
Buffer SK	15 mL
Wash Solution A	18 mL
Elution Buffer E	6 mL
Micro Spin Columns	25
Collection Tubes	25
Elution Tubes (1.7 mL)	25
Product Insert	1

Protocols:

(22550) Urine Exfoliated Cell and Bacteria RNA Purification Kit - Protocol (25 prep)

Safety Data Sheets:

(22550) Urine Exfoliated Cell and Bacteria RNA Purification Kit - Safety Data Sheet

Posters:

Explore the World of RNA - Silicon Carbide vs Silica

Title	Integrated RNA and metabolite profiling of urine liquid biopsies for prostate cancer biomarker discovery.
Journal	Scientific Reports. Volume 10, Article: 3716. 2020.
Authors	Lee, B., Mahmud, I., Marchica, J. et al.

Title	Next Generation Sequencing of urine exfoliated cells: an approach of prostate cancer microRNAs research.
Journal	Scientific Reports. Volume 8, Article: 7111. 2018.
Authors	Guelfi, G., Cochetti, G., Stefanetti, V. et al.

Title	Development of a Voided Urine Assay for Detecting Prostate Cancer Noninvasively: A Pilot Study
Journal	BJU International. 2017.
Authors	Trabulsi EJ, Tripathi SK, Gomella L, Solomides C, Wickstrom E, Thakur ML

Title	Evaluation of urinary XIAP as a diagnostic biomarker of carcinoma of urinary bladder.
Journal	Tumor Biology. 2014.
Authors	Vaccaro M, Malafronte N, Alfieri M, Tommasi N, Leone A.

Title	Long Noncoding RNAs as Putative Biomarkers for Prostate Cancer Detection.
Journal	The Journal of Molecular Diagnostics. 2014.
Authors	Bongyong Lee, Joseph Mazar, Muhammad N. Aftab, Feng Qi, John Shelley, Jian-Liang Li, Subramaniam Govindarajan, Felipe Valerio, Inoel Rivera, Tadzia Thurn, Tien Anh Tran, Darian Kameh, Vipul Patel‡, Ranjan J. Perera.

Title	A pilot study of urinary microRNA as a biomarker for urothelial cancer.
Journal	Canadian Urolological Association Journal. 2013.
Authors	Snowdon J, Boag S, Feilotter H, Izard J, Siemens DR.

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Urine Exfoliated Cell and Bacteria RNA Purification Kit

This kit provides a rapid spin column method for the isolation and purification of total RNA (including microRNA) from exfoliated cells that have been shed into the urine from the urinary tract. The total RNA is preferentially purified from other cellular components such as proteins without the use of phenol or chloroform. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, as well as from the contaminating species found in urine such as glucose and salts, without the use of phenol or chloroform. The purified RNA is of the highest integrity and can be used in a number of downstream applications including real-time RT-PCR, qRT-PCR, Northern blotting, NGS, and expression array assays. Multiple samples can be processed in 20 minutes.

Background

RNA biomarkers from exfoliated cells can be used as non-invasive tools for a number of diagnostic and research applications including the diagnosis and monitoring of bladder, kidney, or other urinary-tract cancers.

Supporting Data

Figure 1. Isolation and Detection of RNA from the Exfoliated Cells in 50 mL of Urine. RNA was isolated from the exfoliated cells in a 50 mL urine sample obtained from a healthy male without any spiking using Norgen's Urine (Exfoliated Cell) RNA Purification Kit. The bind, wash, and elute procedure was followed, and the purified RNA was recovered in 20 µL of the Elution Buffer. A dilution series of HeLa cells with known concentration (from 1 cell per mL to 1,000 cells per mL) was isolated in parallel as standards. Seven microliters of the 20 µL eluted RNA were then subjected to a 20 µL reverse transcription. One microliter of the reverse transcription was used in a 20 µL real-time PCR reaction with primers to detect the ribosomal S14 transcript. The red lines in the PCR baseline graph above correspond to the HeLa RNA standards, while the blue lines correspond to the successful PCR results when RNA isolated from the exfoliated cells in 50 mL of non-spiked urine was used as the template.

Click for expanded view

Kit Specifications

You have selected: Cat. 22550

Kit Specifications
Maximum Column Binding Capacity	50 μg
Volume of Urine Processed	1-50 mL
Maximum Input of Exfoliated Cells	1 x 10⁶
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Time to Complete 10 Purifications	20 minutes (for Exfoliated Cells) 30 minutes (for Bacteria)
Average Yield	~1μg RNA per 1 x 10⁵ cells (For Exfoliated Cells - Varies due to cell density sample) ~0.5μg RNA per 1 x 10⁷ cells (For Bacteria - Varies due to cell density sample)

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 22550 (25 preps)
Buffer SK	15 mL
Wash Solution A	18 mL
Elution Buffer E	6 mL
Micro Spin Columns	25
Collection Tubes	25
Elution Tubes (1.7 mL)	25
Product Insert	1

Protocols:

(22550) Urine Exfoliated Cell and Bacteria RNA Purification Kit - Protocol (25 prep)

Safety Data Sheets:

(22550) Urine Exfoliated Cell and Bacteria RNA Purification Kit - Safety Data Sheet

Posters:

Explore the World of RNA - Silicon Carbide vs Silica

Title	Integrated RNA and metabolite profiling of urine liquid biopsies for prostate cancer biomarker discovery.
Journal	Scientific Reports. Volume 10, Article: 3716. 2020.
Authors	Lee, B., Mahmud, I., Marchica, J. et al.

Title	Next Generation Sequencing of urine exfoliated cells: an approach of prostate cancer microRNAs research.
Journal	Scientific Reports. Volume 8, Article: 7111. 2018.
Authors	Guelfi, G., Cochetti, G., Stefanetti, V. et al.

Title	Development of a Voided Urine Assay for Detecting Prostate Cancer Noninvasively: A Pilot Study
Journal	BJU International. 2017.
Authors	Trabulsi EJ, Tripathi SK, Gomella L, Solomides C, Wickstrom E, Thakur ML

Title	Evaluation of urinary XIAP as a diagnostic biomarker of carcinoma of urinary bladder.
Journal	Tumor Biology. 2014.
Authors	Vaccaro M, Malafronte N, Alfieri M, Tommasi N, Leone A.

Title	Long Noncoding RNAs as Putative Biomarkers for Prostate Cancer Detection.
Journal	The Journal of Molecular Diagnostics. 2014.
Authors	Bongyong Lee, Joseph Mazar, Muhammad N. Aftab, Feng Qi, John Shelley, Jian-Liang Li, Subramaniam Govindarajan, Felipe Valerio, Inoel Rivera, Tadzia Thurn, Tien Anh Tran, Darian Kameh, Vipul Patel‡, Ranjan J. Perera.

Title	A pilot study of urinary microRNA as a biomarker for urothelial cancer.
Journal	Canadian Urolological Association Journal. 2013.
Authors	Snowdon J, Boag S, Feilotter H, Izard J, Siemens DR.

At Norgen Biotek Corp. we are doing our part in response to the global coronavirus SARS-CoV-2 outbreak. | Find out how

Urine Exfoliated Cell and Bacteria RNA Purification Kit (Cat. 22550)

Urine Exfoliated Cell and Bacteria RNA Purification Kit

Urine Exfoliated Cell and Bacteria RNA Purification Kit

Kit Size

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At Norgen Biotek Corp. we are doing our part in response to the global coronavirus SARS-CoV-2 outbreak. | Find out how

Urine Exfoliated Cell and Bacteria RNA Purification Kit (Cat. 22550)

Urine Exfoliated Cell and Bacteria RNA Purification Kit

Urine Exfoliated Cell and Bacteria RNA Purification Kit

Kit Size

Product Overview

Documentation

Citations

Related Products

microRNA (cel-miR-39) Spike-In Kit

Cytoplasmic and Nuclear RNA Purification Kit

Leukocyte RNA Purification Kits

Total RNA Purification Kits

RNase-Free DNase I Kit