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Urine Exfoliated Cell and Bacteria RNA Purification Kit
Purify all sizes of RNA (including microRNA) without the need for phenol
Isolate and detect total RNA from 1 mL and up to 50 mL urine
Provides high-quality RNA for sensitive applications - isolate RNA from as little as 100 cells
Rapid processing time from sampling to downstream testing
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
This kit provides a rapid spin column method for the isolation and purification of total RNA (including microRNA) from exfoliated cells that have been shed into the urine from the urinary tract. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, as well as from the contaminating species found in urine such as glucose and salts, without the use of phenol or chloroform. The purified RNA is of the highest integrity and can be used in a number of downstream applications including real-time RT-PCR, qRT-PCR, Northern blotting, NGS, and expression array assays. Multiple samples can be processed in 20 minutes.
RNA biomarkers from exfoliated cells can be used as non-invasive tools for a number of diagnostic and research applications including the diagnosis and monitoring of bladder, kidney, or other urinary-tract cancers.
Figure 1. Isolation and Detection of RNA from the Exfoliated Cells in 50 mL of Urine. RNA was isolated from the exfoliated cells in a 50 mL urine sample obtained from a healthy male without any spiking using Norgen's Urine (Exfoliated Cell) RNA Purification Kit. The bind, wash, and elute procedure was followed, and the purified RNA was recovered in 20 µL of the Elution Buffer. A dilution series of HeLa cells with known concentration (from 1 cell per mL to 1,000 cells per mL) was isolated in parallel as standards. Seven microliters of the 20 µL eluted RNA were then subjected to a 20 µL reverse transcription. One microliter of the reverse transcription was used in a 20 µL real-time PCR reaction with primers to detect the ribosomal S14 transcript. The red lines in the PCR baseline graph above correspond to the HeLa RNA standards, while the blue lines correspond to the successful PCR results when RNA isolated from the exfoliated cells in 50 mL of non-spiked urine was used as the template.