Urine Exosome and Free-Circulating RNA Isolation Kits

Cat. 59200, 59300, 59400
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Urine Exosome and Free-Circulating RNA Isolation Kits

Cat. 59200, 59300, 59400

All-in-one system for the purification of exosomes and the sequential isolation of RNA and free-circulating RNA from urine samples

  • Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias
  • Versatile sample input ranges
  • Isolate all sizes of free-circulating RNA, including microRNA​
  • Bind and elute all RNA irrespective of size or GC content, without bias
  • The purified exosomal RNA is free from any circulating RNA-binding proteins
  • No phenol extractions, Proteinase K treatment nor carrier RNA required
  • No time-consuming ultracentrifugation, filtration nor special syringes are required
  • No precipitation reagents nor overnight incubation required
  • Concentrate isolated exosomal RNA and free-circulating RNA into a flexible elution volume ranging from 50 µL to 100 µL
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

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Urine Exosome and Free-Circulating RNA Isolation Mini Kit
(Cat. 59200)
50 preps
Urine Exosome and Free-Circulating RNA Isolation Midi Kit
(Cat. 59300)
25 preps
Urine Exosome and Free-Circulating RNA Isolation Maxi Kit
(Cat. 59400)
15 preps

Kit Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Kit Size

  • Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias
  • Versatile sample input ranges
  • Isolate all sizes of free-circulating RNA, including microRNA​
  • Bind and elute all RNA irrespective of size or GC content, without bias
  • The purified exosomal RNA is free from any circulating RNA-binding proteins
  • No phenol extractions, Proteinase K treatment nor carrier RNA required
  • No time-consuming ultracentrifugation, filtration nor special syringes are required
  • No precipitation reagents nor overnight incubation required
  • Concentrate isolated exosomal RNA and free-circulating RNA into a flexible elution volume ranging from 50 µL to 100 µL
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Related Services

Urine Exosome and Free-Circulating RNA Isolation Kits

These kits provide a fast, reliable and convenient method to sequentially isolate and concentrate exosomal RNA as well as Free-Circulating RNA from different urine sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. These kits are designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits since they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kits allow the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. Moreover, the free-circulating, protein-bound, RNA is free from any exosomal RNA. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Urine Exosome and Free-Circulating RNA Isolation Mini Kit

For sample volumes ranging from 250 µL to 1 mL.

Urine Exosome and Free-Circulating RNA Isolation Midi Kit

For sample volumes ranging from 2 mL to 10 mL.

Urine Exosome and Free-Circulating RNA Isolation Maxi Kit

For sample volumes ranging from 11 mL to 30 mL.

Kit Specifications

You have selected: Cat. 59200
Kit Specifications
Minimum Urine Input
250 µL
Maximum Urine Input
1 mL
Size of RNA Purified All sizes, including miRNA
and small RNA (< 200 nt)
Elution Volume 50 - 100 µL
Time to Complete 10 Purifications
35 - 40 minutes
Average Yields* Variable depending on specimen

*Please check page 4 of the product insert for the average yields and the common RNA quantification methods.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Important Note
Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.

You have selected: Cat. 59300
Kit Specifications
Minimum Urine Input
2 mL
Maximum Urine Input
10 mL
Size of RNA Purified All sizes, including miRNA
and small RNA (< 200 nt)
Elution Volume 50 - 100 µL
Time to Complete 10 Purifications
35 - 40 minutes
Average Yields* Variable depending on the specimen

*Please check page 5 of the product insert for the average yields and the common RNA quantification methods.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Important Note
Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.

You have selected: Cat. 59400
Kit Specifications
Minimum Urine Input
11 mL
Maximum Urine Input
30 mL
Size of RNA Purified All sizes, including miRNA
and small RNA (< 200 nt)
Elution Volume 50 - 100 µL
Time to Complete 10 Purifications
40 - 45 minutes
Average Yields* Variable depending on the specimen

*Please check page 5 of the product insert for the average yields and the common RNA quantification methods.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipping. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Important Note
Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.

Supporting Data

Click for expanded view

Component Cat. 59200 (50 preps) Cat. 59300 (25 preps) Cat. 59400 (15 preps)
Slurry E 12.5 mL 12.5 mL 12.5 mL
ExoC Buffer 8 mL 30 mL 50 mL
ExoR Buffer 12 mL 12 mL 12 mL
Lysis Buffer A 2 x 20 mL 2 x 20 mL 2 x 20 mL
Lysis Additive B 2 mL 2 mL 2 mL
Wash Solution A 2 x 18 mL 18 mL 18 mL
Elution Solution A 2 x 6 mL 6 mL 6 mL
Mini Filter Spin Columns 50 25 15
Mini Spin Columns 100 50 30
Collection Tubes 100 50 30
Elution Tubes (1.7 mL) 100 50 30
Product Insert 1 1 1

Urine Exosome and Free-Circulating RNA Isolation Kits

These kits provide a fast, reliable and convenient method to sequentially isolate and concentrate exosomal RNA as well as Free-Circulating RNA from different urine sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. These kits are designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits since they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kits allow the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. Moreover, the free-circulating, protein-bound, RNA is free from any exosomal RNA. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Urine Exosome and Free-Circulating RNA Isolation Mini Kit

For sample volumes ranging from 250 µL to 1 mL.

Urine Exosome and Free-Circulating RNA Isolation Midi Kit

For sample volumes ranging from 2 mL to 10 mL.

Urine Exosome and Free-Circulating RNA Isolation Maxi Kit

For sample volumes ranging from 11 mL to 30 mL.

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 59200
Kit Specifications
Minimum Urine Input
250 µL
Maximum Urine Input
1 mL
Size of RNA Purified All sizes, including miRNA
and small RNA (< 200 nt)
Elution Volume 50 - 100 µL
Time to Complete 10 Purifications
35 - 40 minutes
Average Yields* Variable depending on specimen

*Please check page 4 of the product insert for the average yields and the common RNA quantification methods.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Important Note
Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.

You have selected: Cat. 59300
Kit Specifications
Minimum Urine Input
2 mL
Maximum Urine Input
10 mL
Size of RNA Purified All sizes, including miRNA
and small RNA (< 200 nt)
Elution Volume 50 - 100 µL
Time to Complete 10 Purifications
35 - 40 minutes
Average Yields* Variable depending on the specimen

*Please check page 5 of the product insert for the average yields and the common RNA quantification methods.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Important Note
Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.

You have selected: Cat. 59400
Kit Specifications
Minimum Urine Input
11 mL
Maximum Urine Input
30 mL
Size of RNA Purified All sizes, including miRNA
and small RNA (< 200 nt)
Elution Volume 50 - 100 µL
Time to Complete 10 Purifications
40 - 45 minutes
Average Yields* Variable depending on the specimen

*Please check page 5 of the product insert for the average yields and the common RNA quantification methods.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipping. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Important Note
Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.

Component Cat. 59200 (50 preps) Cat. 59300 (25 preps) Cat. 59400 (15 preps)
Slurry E 12.5 mL 12.5 mL 12.5 mL
ExoC Buffer 8 mL 30 mL 50 mL
ExoR Buffer 12 mL 12 mL 12 mL
Lysis Buffer A 2 x 20 mL 2 x 20 mL 2 x 20 mL
Lysis Additive B 2 mL 2 mL 2 mL
Wash Solution A 2 x 18 mL 18 mL 18 mL
Elution Solution A 2 x 6 mL 6 mL 6 mL
Mini Filter Spin Columns 50 25 15
Mini Spin Columns 100 50 30
Collection Tubes 100 50 30
Elution Tubes (1.7 mL) 100 50 30
Product Insert 1 1 1
 


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