• Isolate all sizes of exosomal and extracellular vesicle RNA, including microRNA
  • ​Versatile sample input ranging from 2 mL to 10 mL
  • Isolate all sizes of free-circulating RNA, including microRNA​
  • Bind and elute all RNA irrespective of size or GC content, without bias
  • The purified exosomal RNA is free from any circulating RNA-binding proteins
  • No phenol extractions, Proteinase K treatment nor carrier RNA required
  • No time-consuming ultracentrifugation, filtration nor special syringes are required
  • No precipitation reagents nor overnight incubation required
  • Concentrate isolated exosomal RNA and free-circulating RNA into a flexible elution volume ranging from 50 µL to 100 µL
  • Purify high-quality RNA in 15-20 minutes

ItemsCat. #Size
Urine Exosome and Free-Circulating RNA Isolation Midi Kit 59300 25 preps

Urine Exosome and Free-Circulating RNA Isolation Midi Kit

This kit provides a fast, reliable and convenient method to sequentially isolate and concentrate exosomal RNA as well as Free-Circulating RNA from different urine sample volumes ranging from 2 mL to 10 mL. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The kit is designed to isolate all sizes of extracellular vesicle RNA, including microRNA as well as all sizes of the free-circulating protein-bound RNA, including microRNA.

The kit provides a clear advantage over other available kits since they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. Moreover, the free-circulating, protein-bound, RNA is free from any exosomal RNA. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Kit Specifications
Minimum Urine Input
2 mL
Maximum Urine Input
10 mL
Size of RNA Purified All sizes, including miRNA
and small RNA (< 200 nt)
Elution Volume 50 - 100 µL
Time to Complete 10 Purifications
35 - 40 minutes
Average Yields* Variable depending on specimen

*Please check page 5 of the product insert for the average yields and the common RNA quantification methods.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 years without showing any reduction in performance. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Important Note
Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.


Kit Components
Component Cat. 59300 (25 preps)
Slurry E 12.5 mL
ExoC Buffer 8 mL
ExoR Buffer 12 mL
Lysis Buffer A 2 x 20 mL
Lysis Additive B 2 mL
Wash Solution A 18 mL
Elution Solution A 6 mL
Mini Filter Spin Columns 25
Mini Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1