Urine Exosome Purification and RNA Isolation Kits

Cat. 58400, 58700, 58800
  • Professional Tech Support with Senior R&D Scientist
  • Fast and Flexible Ordering Options (online, email, or phone)
  • Volume Discounts Available on Large Quantity Orders*

*Inquire by phone or email for more information.


Urine Exosome Purification and RNA Isolation Kits

Cat. 58400, 58700, 58800

All-in-one system for the purification of exosomes and the subsequent isolation of RNA from urine samples

  • Purification and enrichment of intact urinary exosomes for functional studies
  • Bind and elute all RNA irrespective of size or GC content, without bias
  • Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias.
  • Versatile urine input volumes
  • No phenol extractions, Proteinase K treatment, nor carrier RNA required
  • No time-consuming ultracentrifugation, filtration nor special syringes are required
  • No precipitation reagents nor overnight incubation required
  • Compatible with urine from any species
  • Pure exosomes are purified and are free-from any other RNA-binding proteins
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

Want a discount?

Create your account to receive 15% off on your first purchase!

Urine Exosome Purification and RNA Isolation Mini Kit
(Cat. 58400)
50 preps
Urine Exosome Purification and RNA Isolation Midi Kit
(Cat. 58700)
25 preps
Urine Exosome Purification and RNA Isolation Maxi Kit
(Cat. 58800)
15 preps

Kit Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Kit Size

  • Purification and enrichment of intact urinary exosomes for functional studies
  • Bind and elute all RNA irrespective of size or GC content, without bias
  • Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias.
  • Versatile urine input volumes
  • No phenol extractions, Proteinase K treatment, nor carrier RNA required
  • No time-consuming ultracentrifugation, filtration nor special syringes are required
  • No precipitation reagents nor overnight incubation required
  • Compatible with urine from any species
  • Pure exosomes are purified and are free-from any other RNA-binding proteins
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Urine Exosome Purification and RNA Isolation Kits

Norgen’s Exosome Purification and RNA Isolation Kits constitute all-in-one systems for the purification of exosomes and the subsequent isolation of exosomal RNA from different urine sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. These kits are designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The purified RNA is free from any protein-bound circulating RNA and of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Urine Exosome Purification and RNA Isolation Mini Kit

For sample volumes ranging from 250 µL to 1 mL.

Urine Exosome Purification and RNA Isolation Midi Kit

For sample volumes ranging from 2 mL to 10 mL.

Urine Exosome Purification and RNA Isolation Maxi Kit

For sample volumes ranging from 11 mL to 30 mL.

Kit Specifications

You have selected: Cat. 58400
Kit Specifications
Minimum Urine Input
250 μL
Maximum Urine Input
1 mL
Size of Exosomes Purified
40 nm - 150 nm
Size of RNA Purified All sizes, including miRNA
and small RNA (< 200 nt)
Elution Volume 50-100 μL
Time to Complete 10 Purifications
35 - 40 minutes
Average Yields* Variable depending on specimen

*Please check page 4 of the product insert for the average yields and the common RNA quantification methods.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Important Note
Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.

You have selected: Cat. 58700
Kit Specifications
Minimum Urine Input
2 mL
Maximum Urine Input
10 mL
Size of Exosomes Purified
40 nm - 150 nm
Size of RNA Purified All sizes, including miRNA
and small RNA (< 200 nt)
Elution Volume 50-100 μL
Time to Complete 10 Purifications
35 - 40 minutes
Average Yields* Variable depending on specimen

*Please check page 4 of the product insert for the average yields and the common RNA quantification methods.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Important Note
Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.

You have selected: Cat. 58800
Kit Specifications
Minimum Urine Input
11 mL
Maximum Urine Input
30 mL
Size of Exosomes Purified
40 nm - 150 nm
Size of RNA Purified All sizes, including miRNA
and small RNA (< 200 nt)
Elution Volume 50-100 μL
Time to Complete 10 Purifications
35 - 40 minutes
Average Yields* Variable depending on specimen

*Please check page 4 of the product insert for the average yields and the common RNA quantification methods

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Important Note
Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.

Supporting Data

Click for expanded view

Component Cat. 58400 (50 preps) Cat. 58700 (25 preps) Cat. 58800 (15 preps)
Slurry E 12.5 mL 12.5 mL 12.5 mL
ExoC Buffer 8 mL 30 mL 50 mL
ExoR Buffer 12 mL 12 mL 12 mL
Lysis Buffer A 20 mL 20 mL 20 mL
Lysis Additive B 2 mL 2 mL 2 mL
Wash Solution A 18 mL 18 mL 18 mL
Elution Solution A 6 mL 6 mL 6 mL
Mini Filter Spin Columns 50 25 15
Mini Spin Columns 50 25 15
Collection Tubes 50 25 15
Elution Tubes (1.7 mL) 100 50 30
Product Insert 1 1 1

Midi (58700)

Title Placental exosomes isolated from urine of patients with gestational diabetes exhibit a differential profile expression of microRNAs across gestation
Journal International Journal of Molecular Medicine. 2020.
Authors Ana Sofía Herrera-Van Oostdam Juan Carlos Toro-Ortíz, et al.

Maxi (58800)

Title A Pilot Study of miRNA Expression Profile as a Liquid Biopsy for Full-Marathon Participants
Journal Sports. 2021.
Authors Tomoaki Kuji, Takehito Sugasawa, Shin-ichiro Fujita, Seiko Ono, Yasushi Kawakami and Kazuhiro Takekoshi

Urine Exosome Purification and RNA Isolation Kits

Norgen’s Exosome Purification and RNA Isolation Kits constitute all-in-one systems for the purification of exosomes and the subsequent isolation of exosomal RNA from different urine sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. These kits are designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The purified RNA is free from any protein-bound circulating RNA and of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Urine Exosome Purification and RNA Isolation Mini Kit

For sample volumes ranging from 250 µL to 1 mL.

Urine Exosome Purification and RNA Isolation Midi Kit

For sample volumes ranging from 2 mL to 10 mL.

Urine Exosome Purification and RNA Isolation Maxi Kit

For sample volumes ranging from 11 mL to 30 mL.

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 58400
Kit Specifications
Minimum Urine Input
250 μL
Maximum Urine Input
1 mL
Size of Exosomes Purified
40 nm - 150 nm
Size of RNA Purified All sizes, including miRNA
and small RNA (< 200 nt)
Elution Volume 50-100 μL
Time to Complete 10 Purifications
35 - 40 minutes
Average Yields* Variable depending on specimen

*Please check page 4 of the product insert for the average yields and the common RNA quantification methods.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Important Note
Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.

You have selected: Cat. 58700
Kit Specifications
Minimum Urine Input
2 mL
Maximum Urine Input
10 mL
Size of Exosomes Purified
40 nm - 150 nm
Size of RNA Purified All sizes, including miRNA
and small RNA (< 200 nt)
Elution Volume 50-100 μL
Time to Complete 10 Purifications
35 - 40 minutes
Average Yields* Variable depending on specimen

*Please check page 4 of the product insert for the average yields and the common RNA quantification methods.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Important Note
Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.

You have selected: Cat. 58800
Kit Specifications
Minimum Urine Input
11 mL
Maximum Urine Input
30 mL
Size of Exosomes Purified
40 nm - 150 nm
Size of RNA Purified All sizes, including miRNA
and small RNA (< 200 nt)
Elution Volume 50-100 μL
Time to Complete 10 Purifications
35 - 40 minutes
Average Yields* Variable depending on specimen

*Please check page 4 of the product insert for the average yields and the common RNA quantification methods

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Important Note
Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.

Component Cat. 58400 (50 preps) Cat. 58700 (25 preps) Cat. 58800 (15 preps)
Slurry E 12.5 mL 12.5 mL 12.5 mL
ExoC Buffer 8 mL 30 mL 50 mL
ExoR Buffer 12 mL 12 mL 12 mL
Lysis Buffer A 20 mL 20 mL 20 mL
Lysis Additive B 2 mL 2 mL 2 mL
Wash Solution A 18 mL 18 mL 18 mL
Elution Solution A 6 mL 6 mL 6 mL
Mini Filter Spin Columns 50 25 15
Mini Spin Columns 50 25 15
Collection Tubes 50 25 15
Elution Tubes (1.7 mL) 100 50 30
Product Insert 1 1 1

Midi (58700)

Title Placental exosomes isolated from urine of patients with gestational diabetes exhibit a differential profile expression of microRNAs across gestation
Journal International Journal of Molecular Medicine. 2020.
Authors Ana Sofía Herrera-Van Oostdam Juan Carlos Toro-Ortíz, et al.

Maxi (58800)

Title A Pilot Study of miRNA Expression Profile as a Liquid Biopsy for Full-Marathon Participants
Journal Sports. 2021.
Authors Tomoaki Kuji, Takehito Sugasawa, Shin-ichiro Fujita, Seiko Ono, Yasushi Kawakami and Kazuhiro Takekoshi

 


Related Products