Urine Exosome Purification and RNA Isolation Kits (Cat. 58400, 58700, 58800)
Urine Exosome Purification and RNA Isolation Kits
![Urine Exosome Purification and RNA Isolation Mini Kit](https://norgenbiotek.com/sites/default/files/styles/original/public/images/product_images/58400-Urine%20Exosome%20Purification%20and%20RNA%20Mini%20%20WEB.jpg?itok=reKtZy4t" "Urine Exosome Purification and RNA Isolation Mini Kit")
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Urine Exosome Purification and RNA Isolation Kits
All-in-one system for the purification of exosomes and the subsequent isolation of RNA from urine samples
- Purification and enrichment of intact urinary exosomes for functional studies
- Bind and elute all RNA irrespective of size or GC content, without bias
- Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias.
- Versatile urine input volumes
- No phenol extractions, Proteinase K treatment, nor carrier RNA required
- No time-consuming ultracentrifugation, filtration nor special syringes are required
- No precipitation reagents nor overnight incubation required
- Compatible with urine from any species
- Pure exosomes are purified and are free-from any other RNA-binding proteins
- Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
- The purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
For research use only and NOT intended for in vitro diagnostics.
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(Cat. 58400) 50 preps
(Cat. 58700) 25 preps
(Cat. 58800) 15 preps
Product Format Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.
Product Size
Kit Specifications
|
Kit Specifications
|
|
| Minimum Urine Input |
250 μL
|
|
Maximum Urine Input
|
1 mL
|
|
Size of Exosomes Purified
|
40 nm - 150 nm
|
| Size of RNA Purified | All sizes, including miRNA and small RNA (< 200 nt) |
| Elution Volume | 50-100 μL |
| Time to Complete 10 Purifications |
35 - 40 minutes
|
| Average Yields* | Variable depending on specimen |
*Please check page 4 of the product insert for the average yields and the common RNA quantification methods.
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
Important Note
Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.
|
Kit Specifications
|
|
| Minimum Urine Input |
2 mL
|
|
Maximum Urine Input
|
10 mL
|
|
Size of Exosomes Purified
|
40 nm - 150 nm
|
| Size of RNA Purified | All sizes, including miRNA and small RNA (< 200 nt) |
| Elution Volume | 50-100 μL |
| Time to Complete 10 Purifications |
35 - 40 minutes
|
| Average Yields* | Variable depending on specimen |
*Please check page 4 of the product insert for the average yields and the common RNA quantification methods.
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
Important Note
Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.
|
Kit Specifications
|
|
| Minimum Urine Input |
11 mL
|
|
Maximum Urine Input
|
30 mL
|
|
Size of Exosomes Purified
|
40 nm - 150 nm
|
| Size of RNA Purified | All sizes, including miRNA and small RNA (< 200 nt) |
| Elution Volume | 50-100 μL |
| Time to Complete 10 Purifications |
35 - 40 minutes
|
| Average Yields* | Variable depending on specimen |
*Please check page 4 of the product insert for the average yields and the common RNA quantification methods
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
Important Note
Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.
Supporting Data
Midi (58700)
| Title | Placental exosomes isolated from urine of patients with gestational diabetes exhibit a differential profile expression of microRNAs across gestation |
| Journal | International Journal of Molecular Medicine. 2020. |
| Authors | Ana Sofía Herrera-Van Oostdam Juan Carlos Toro-Ortíz, et al. |
Maxi (58800)
| Title | A Pilot Study of miRNA Expression Profile as a Liquid Biopsy for Full-Marathon Participants |
| Journal | Sports. 2021. |
| Authors | Tomoaki Kuji, Takehito Sugasawa, Shin-ichiro Fujita, Seiko Ono, Yasushi Kawakami and Kazuhiro Takekoshi |
Urine Exosome Purification and RNA Isolation Kits
Norgen’s Exosome Purification and RNA Isolation Kits constitute all-in-one systems for the purification of exosomes and the subsequent isolation of exosomal RNA from different urine sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. These kits are designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The purified RNA is free from any protein-bound circulating RNA and of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Urine Exosome Purification and RNA Isolation Mini Kit
For sample volumes ranging from 250 µL to 1 mL.
Urine Exosome Purification and RNA Isolation Midi Kit
For sample volumes ranging from 2 mL to 10 mL.
Urine Exosome Purification and RNA Isolation Maxi Kit
For sample volumes ranging from 11 mL to 30 mL.
Supporting Data
Kit Specifications
|
Kit Specifications
|
|
| Minimum Urine Input |
250 μL
|
|
Maximum Urine Input
|
1 mL
|
|
Size of Exosomes Purified
|
40 nm - 150 nm
|
| Size of RNA Purified | All sizes, including miRNA and small RNA (< 200 nt) |
| Elution Volume | 50-100 μL |
| Time to Complete 10 Purifications |
35 - 40 minutes
|
| Average Yields* | Variable depending on specimen |
*Please check page 4 of the product insert for the average yields and the common RNA quantification methods.
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
Important Note
Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.
|
Kit Specifications
|
|
| Minimum Urine Input |
2 mL
|
|
Maximum Urine Input
|
10 mL
|
|
Size of Exosomes Purified
|
40 nm - 150 nm
|
| Size of RNA Purified | All sizes, including miRNA and small RNA (< 200 nt) |
| Elution Volume | 50-100 μL |
| Time to Complete 10 Purifications |
35 - 40 minutes
|
| Average Yields* | Variable depending on specimen |
*Please check page 4 of the product insert for the average yields and the common RNA quantification methods.
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
Important Note
Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.
|
Kit Specifications
|
|
| Minimum Urine Input |
11 mL
|
|
Maximum Urine Input
|
30 mL
|
|
Size of Exosomes Purified
|
40 nm - 150 nm
|
| Size of RNA Purified | All sizes, including miRNA and small RNA (< 200 nt) |
| Elution Volume | 50-100 μL |
| Time to Complete 10 Purifications |
35 - 40 minutes
|
| Average Yields* | Variable depending on specimen |
*Please check page 4 of the product insert for the average yields and the common RNA quantification methods
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
Important Note
Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.
Midi (58700)
| Title | Placental exosomes isolated from urine of patients with gestational diabetes exhibit a differential profile expression of microRNAs across gestation |
| Journal | International Journal of Molecular Medicine. 2020. |
| Authors | Ana Sofía Herrera-Van Oostdam Juan Carlos Toro-Ortíz, et al. |
Maxi (58800)
| Title | A Pilot Study of miRNA Expression Profile as a Liquid Biopsy for Full-Marathon Participants |
| Journal | Sports. 2021. |
| Authors | Tomoaki Kuji, Takehito Sugasawa, Shin-ichiro Fujita, Seiko Ono, Yasushi Kawakami and Kazuhiro Takekoshi |
