Urine Exosome Purification and RNA Isolation Kits All-in-one system for the purification of exosomes and the subsequent isolation of RNA from urine samples For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Urine Exosome Purification and RNA Isolation Kits All-in-one system for the purification of exosomes and the subsequent isolation of RNA from urine samples Price USD$1,405.00 SKU 58400 Format Mini Midi Maxi Size 50 Preps Quantity Overview Details Documentation Citations Overview Purification and enrichment of intact urinary exosomes for functional studies Bind and elute all RNA irrespective of size or GC content, without bias Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. Versatile urine input volumes No phenol extractions, Proteinase K treatment, nor carrier RNA required No time-consuming ultracentrifugation, filtration nor special syringes are required No precipitation reagents nor overnight incubation required Compatible with urine from any species Pure exosomes are purified and are free-from any other RNA-binding proteins Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix The purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services Norgen’s Exosome Purification and RNA Isolation Kits constitute all-in-one systems for the purification of exosomes and the subsequent isolation of exosomal RNA from different urine sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. These kits are designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The purified RNA is free from any protein-bound circulating RNA and of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays. Urine Exosome Purification and RNA Isolation Mini Kit For sample volumes ranging from 250 µL to 1 mL. Urine Exosome Purification and RNA Isolation Midi Kit For sample volumes ranging from 2 mL to 10 mL. Urine Exosome Purification and RNA Isolation Maxi Kit For sample volumes ranging from 11 mL to 30 mL. Details Supporting Data Previous Figure 1. Isolation of RNA from exosomes purified from different urine volumes. Norgen's Urine Exosome Purification and RNA Isolation Mini Kit (Cat# 58400) was used to isolate RNA from exosomes purified from different urine volumes using the same kit. Two microlitres of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated urinary exosomal miR-30a. (A) The urinary exosomal miR-30a is linearly decreasing with increasing the sample input volume. B) The relative amount of the urinary exosomal miR-30a shows excellent linearity with a percentage of recovery of more than 90%. Figure 2. Intact exosomes purified from 5 mL of urine using different purification methods. Intact exosomes were purified from 5 mL urine using Norgen's Urine Exosome Purification and RNA Isolation Midi Kit (Cat# 58700), Competitor S's kit, and Competitor L's kit. Exosomes purified using Norgen's kit were resuspended in 400 µL Norgen's ExoR buffer, diluted 1:1,000 and visualized on the NanoSight LM10 instrument. The analysis shows that Norgens kit isolated 115 nm exosomes with a recovery of 8.36 x 109 particles/mL urine samples. No impurities were found to be contaminating the exosomes purified using Norgen's Urine Exosome Purification and RNA Isolation Midi kit. Additionally, exosomes with a broader size range covering from 75nm to 250nm were purified from 5 mL urine with a higher concentration as compared to the other two methods. Figure 3. Intact Exosomes were purified from 10 mL urine using Norgen's Urine Exosome Purification and RNA Isolation Midi Kit (Cat# 58700) and ultracentrifugation. Exosomes purified using Norgens kit and ultracentrifugation was resuspended in 400 µL of Norgens ExoR buffer, diluted 1:1,000 and visualized on the NanoSight LM10 instrument. The analysis shows that Norgens kit purified exosomes with sizes ranging from 65 nm - 195 nm, with a total recovery of 7.63 x 108 particles/mL. No impurities were found to be contaminating the exosomes purified using Norgen's Urine Exosome Purification and RNA Isolation Midi Kit as opposed to the exosomes purified using ultracentrifugation, which purified exosomes with larger particle sizes ranging from 125 nm to 235 nm with a total recovery of 1.56 x 108 particles/mL. Figure 4. Isolation of RNA from exosomes purified from different urine volumes. Norgen's Urine Exosome Purification and RNA Isolation Midi Kit (Cat# 58700) was used to isolate RNA from exosomes purified from different urine volumes using the same kit. Two microlitres of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated urinary exosomal miR-30a. (A) The urinary exosomal miR-30a is linearly decreasing with increasing the sample input volume. B) The relative amount of the urinary exosomal miR-30a shows excellent linearity with a percentage of recovery of more than 90%. Figure 5. Intact exosomes purified from 5 mL using different purification methods. Intact exosomes were purified from 5 mL urine using Norgen's Urine Exosome Purification and RNA Isolation Midi Kit (Cat# 58700), Competitor S's kit, and Competitor L's kit. Exosomes purified using Norgen's kit were resuspended in 400 µL of Norgen's ExoR buffer, diluted 1:1,000 and visualized on the NanoSight LM10 instrument. The analysis shows that Norgen's kit isolated 115 nm exosomes with a recovery of 8.36 x 109 particles/mL urine samples. No impurities were found to be contaminating the exosomes purified using Norgen's Urine Exosome Purification and RNA Isolation Midi Kit. Additionally, exosomes with a broader size range covering from 75nm to 250nm were purified from 5 mL urine with a higher concentration as compared to the other two methods. Figure 6. Intact Exosomes were purified from 10 mL urine using Norgen's Urine Exosome Purification and RNA Isolation Midi Kit (Cat# 58700) and ultracentrifugation. Exosomes purified using Norgen's kit and ultracentrifugation were resuspended in 400 µL of Norgen's ExoR buffer, diluted 1:1,000 and visualized on the NanoSight LM10 instrument. The analysis shows that Norgen's kit purified exosomes with sizes ranging from 65 nm to 195 nm, with a total recovery of 7.63 x 108 particles/mL. No impurities were found to be contaminating the exosomes purified using Norgen's Urine Exosome Purification and RNA Isolation Midi Kit, as opposed to the exosomes purified using ultracentrifugation which purified exosomes with larger particle sizes ranging from 125 nm to 235 nm with a total recovery of 1.56 x 108 particles/mL. Figure 7. Isolation of RNA from exosomes purified from different urine volumes. Norgen's Urine Exosome Purification and RNA Isolation Maxi Kit (Cat# 58800) was used to isolate RNA from exosomes purified from different urine volumes using the same kit. Two microlitres of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated urinary exosomal miR-30a. (A) The urinary exosomal miR-30a is linearly decreasing with increasing the sample input volume. B) The relative amount of the urinary exosomal miR-30a shows excellent linearity with a percentage of recovery of more than 90%. Next Click for expanded view Kit Specifications Minimum Urine Input 250 μL Maximum Urine Input 1 mL Size of Exosomes Purified 40 nm - 150 nm Size of RNA Purified All sizes, including miRNA and small RNA (< 200 nt) Elution Volume 50-100 μL Time to Complete 10 Purifications 35 - 40 minutes Average Yields* Variable depending on specimen *Please check page 4 of the product insert for the average yields and the common RNA quantification methods. Storage Conditions and Product Stability All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed. Important Note Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes. Component Cat. 58400 (50 preps) Cat. 58700 (25 preps) Cat. 58800 (15 preps) Slurry E 12.5 mL 12.5 mL 12.5 mL ExoC Buffer 8 mL 30 mL 50 mL ExoR Buffer 12 mL 12 mL 12 mL Lysis Buffer A 20 mL 20 mL 20 mL Lysis Additive B 2 mL 2 mL 2 mL Wash Solution A 18 mL 18 mL 18 mL Elution Solution A 6 mL 6 mL 6 mL Mini Filter Spin Columns 50 25 15 Mini Spin Columns 50 25 15 Collection Tubes 50 25 15 Elution Tubes (1.7 mL) 100 50 30 Product Insert 1 1 1 Documentation PROTOCOLS PI58400-6 Urine Exosome and RNA Isolation Mini Kit Insert.pdf PI58700-6 Urine Exosome and RNA Isolation Midi Kit Insert.pdf PI58800-6 Urine Exosome and RNA Isolation Maxi Kit Insert.pdf SAFETY DATA SHEETS 58400_58700_58800_SDS.pdf POSTERS FLYERS Exosome Purification Kit v3.0.pdf RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class Citations Powered by Bioz See more details on Bioz Related Products Urine Exosome Purification Mini Kit Exosomal RNA Isolation Kit
Urine Exosome Purification and RNA Isolation Kits All-in-one system for the purification of exosomes and the subsequent isolation of RNA from urine samples For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Urine Exosome Purification and RNA Isolation Kits All-in-one system for the purification of exosomes and the subsequent isolation of RNA from urine samples Price USD$1,405.00 SKU 58400 Format Mini Midi Maxi Size 50 Preps Quantity