• Purification and enrichment of intact urinary exosomes for functional studies
  • Bind and elute all RNA irrespective of size or GC content, without bias
  • Isolate all sizes of exosomal and extracellular vesicle RNA, including microRNA​
  • Versatile urine input volume (250 µL to 1 mL)
  • No phenol extractions, Proteinase K treatment, nor carrier RNA required
  • No time-consuming ultracentrifugation, filtration nor special syringes are required
  • No precipitation reagents nor overnight incubation required
  • Compatible with urine from any species
  • Pure exosomes are purified and are free-from any other RNA-binding proteins

ItemsCat. #Size
Urine Exosome Purification and RNA Isolation Mini Kit 58400 50 preps

Urine Exosome Purification and RNA Isolation Mini Kit

Norgen’s Exosome Purification and RNA Isolation Mini Kit constitutes an all-in-one system for the purification of exosomes and the subsequent isolation of exosomal RNA from different urine sample volumes ranging from 250 µL to 1 mL. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The kit is designed to isolate all sizes of extracellular vesicle RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The purified RNA is free from any protein-bound circulating RNA and of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Kit Specifications
Minimum Urine Input
250 μL
Maximum Urine Input
1 mL
Size of Exosomes Purified
40 nm - 150 nm
Size of RNA Purified All sizes, including miRNA
and small RNA (< 200 nt)
Elution Volume 50-100 μL
Time to Complete 10 Purifications
35 - 40 minutes
Average Yields* Variable depending on specimen

*Please check page 4 of the product insert for the average yields and the common RNA quantification methods.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 years without showing any reduction in performance. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Important Note
Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.


Kit Components
Component Cat. 58400 (50 preps)
Slurry E 12.5 mL
ExoC Buffer 8 mL
ExoR Buffer 12 mL
Lysis Buffer A 20 mL
Lysis Additive B 2 mL
Wash Solution A 18 mL
Elution Solution A 6 mL
Mini Filter Spin Columns 50
Mini Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 100
Product Insert 1