A rapid procedure for the isolation of exosomal RNA from urine sample.
|Urine Exosome RNA Kit||47200||50 Preps|
Urine Exosome RNA Isolation Kit
This kit provides a rapid spin column procedure for the isolation of exosomal RNA from urine samples. Users can simultaneously concentrate and isolate high quality exosomal RNA, including microRNA, for use in sensitive downstream assays such as RT-PCR, qRT-PCR, NGS, microarrays and more. The protocol can be completed in under 30 minutes. Urine volumes of 1 to 10 mL can be processed easily and rapidly. All sizes of RNA are recovered at an equal rate without the need for any phenol steps.
Exosomes are 40 - 100 nm membrane vesicles, which are secreted by most cell types. Exosomes can be found in saliva, blood, urine, amniotic fluid and malignant ascite fluids, among other biological fluids. Evidence has been accumulating recently that these vesicles act as cellular messengers, conveying information to distant cells and tissues within the body. The exosomes contain cell-specific proteins, lipids and RNAs, which are transported to other cells, where they can alter function and/or physiology. These exosomes may play a functional role in mediating adaptive immune responses to infectious agents and tumours, tissue repair, neural communication and transfer of pathogenic proteins. Recent work has demonstrated the presence of distinct subsets of microRNAs within exosomes which depend upon the tumour cell type from which they are secreted. For this reason exosomal RNAs may serve as biomarkers for various diseases including cancer. As the RNA molecules encapsulated within exosomes are protected from degradation by RNAses they can be efficiently recovered from biological fluids, such as urine.
|Minimum Urine Input||
|Maximum Urine Input||
|Size of RNA Purified||
Small exosomal RNA species
|Time to Complete Purification||
~ 50 minutes
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25oC) for up to 1 year without showing any reduction in performance.
|Title||Exosomal lncRNA-p21 levels may help to distinguish prostate cancer from benign disease|
|Journal||Frontiers in Genetics. 2015.|
|Authors||Mustafa Isin, Ege Uysaler, Emre Ozgur, Hikmet Koseoglu, Oner Sanli, Omer Baris Yucel, Ugur Gezer and Nejat Dalay|
|Title||Evaluation of Cell-Free Urine microRNAs Expression for the Use in Diagnosis of Ovarian and Endometrial Cancers. A Pilot Study|
|Journal||Pathology & Oncology Research. 2015.|
|Authors||Luděk Záveský, Eva Jandáková, Radovan Turyna, Lucie Langmeierová, Vít Weinberger, Lenka Záveská Drábková, Martina Hůlková, Aleš Hořínek, Daniela Dušková, Jaroslav Feyereisl, Luboš Minář, Milada Kohoutová|
|Title||Detection of JCPyV microRNA in blood and urine samples of multiple sclerosis patients under natalizumab therapy|
|Journal||Journal of NeuroVirology. 2015.|
|Authors||Giovannelli I, Martelli F, Repice A, Massacesi L, Azzi A, Giannecchini S.|
|Title||Circulating and urinary microRNA profile in focal segmental glomerulosclerosis: a pilot study|
|Journal||European Journal of Clinical Investigation. 2015.|
|Authors||Ramezani A, Devaney JM, Cohen S, Wing MR, Scott R, Knoblach S, Singhal R, Howard L, Kopp JB, Raj DS|
|Title||A Simplified Method to Recover Urinary Vesicles for Clinical Applications, and Sample Banking|
|Journal||Scientific Reports. 2014.|
|Authors||Luca Musante, Dorota Tataruch, Dongfeng Gu, Alberto Benito-Martin, Giulio Calzaferri, Sinead Aherne & Harry Holthofer|
|Title||Emerging technologies in extracellular vesicle-based molecular diagnostics|
|Journal||Expert Review of Molecular Diagnostics. 2014.|
|Authors||Shidong Jia, Davide Zocco, Michael L Samuels, Michael F Chou, Roger Chammas, Johan Skog, Natasa Zarovni, Fatemeh Momen-Heravi, and Winston Patrick Kuo|
|Title||Overexpression of a DENND1A isoform produces a polycystic ovary syndrome theca phenotype.|
|Journal||PNAS Journal. 2014.|
|Authors||McAllister J,Modi B, Miller B, Biegler J, Bruggeman R, Legro R, and Strauss III J.|
|Title||miR-23a is decreased during muscle atrophy by a mechanism that includes calcineurin signaling and exosome-mediated export.|
|Journal||American Journal of Physiology. Cell Physiology. 2014.|
|Authors||Hudson MB, Woodworth-Hobbs ME, Zheng B, Rahnert JA, Blount MA, Gooch JL, Searles CD, Price SR.|
|Title||Characterization and deep sequencing analysis of exosomal and non-exosomal miRNA in human urine.|
|Journal||Urine Exosome RNA Isolation Kit. .|
|Authors||Cheng L, Sun X, Scicluna BJ, Coleman BM, Hill AF.|
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