A rapid procedure for the isolation of exosomal RNA from urine sample

  • Isolation of exosomal RNA molecules from urine samples
  • Rapid and convenient spin-column protocol
  • Isolate inhibitor-free urinary microRNA for any application

ItemsCat. #Size
Urine Exosome RNA Isolation Kit 47200 50 Preps

Urine Exosome RNA Isolation Kit

This kit provides a rapid spin column procedure for the isolation of exosomal RNA from urine samples. Users can simultaneously concentrate and isolate high quality exosomal RNA, including microRNA, for use in sensitive downstream assays such as RT-PCR, qRT-PCR, NGS, microarrays and more. The protocol can be completed in under 30 minutes. Urine volumes of 1 to 10 mL can be processed easily and rapidly. All sizes of RNA are recovered at an equal rate without the need for any phenol steps.


Exosomes are 40 - 150 nm membrane vesicles, which are secreted by most cell types. Exosomes can be found in saliva, blood, urine, amniotic fluid and malignant ascite fluids, among other biological fluids. Evidence has been accumulating recently that these vesicles act as cellular messengers, conveying information to distant cells and tissues within the body. The exosomes contain cell-specific proteins, lipids and RNAs, which are transported to other cells, where they can alter function and/or physiology. These exosomes may play a functional role in mediating adaptive immune responses to infectious agents and tumours, tissue repair, neural communication and transfer of pathogenic proteins. Recent work has demonstrated the presence of distinct subsets of microRNAs within exosomes which depend upon the tumour cell type from which they are secreted. For this reason exosomal RNAs may serve as biomarkers for various diseases including cancer. As the RNA molecules encapsulated within exosomes are protected from degradation by RNAses they can be efficiently recovered from biological fluids, such as urine.

Kit Specifications
Minimum Urine Input
1 mL
Maximum Urine Input
10 mL
Size of RNA Purified
Small exosomal RNA species
Time to Complete Purification
~ 50 minutes

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 1 year without showing any reduction in performance.

Kit Components
Component Cat. 47200 (50 preps)
Slurry B1 22 mL
Binding Buffer A 20 mL
Lysis Buffer A 2 x 20 mL
Wash Solution A 38 mL
Elution Solution A 6 mL
Mini Filter Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1

Title Exosomal lncRNA-p21 levels may help to distinguish prostate cancer from benign disease
Journal Frontiers in Genetics. 2015.
Authors Mustafa Isin, Ege Uysaler, Emre Ozgur, Hikmet Koseoglu, Oner Sanli, Omer Baris Yucel, Ugur Gezer and Nejat Dalay
Title Evaluation of Cell-Free Urine microRNAs Expression for the Use in Diagnosis of Ovarian and Endometrial Cancers. A Pilot Study
Journal Pathology & Oncology Research. 2015.
Authors Luděk Záveský, Eva Jandáková, Radovan Turyna, Lucie Langmeierová, Vít Weinberger, Lenka Záveská Drábková, Martina Hůlková, Aleš Hořínek, Daniela Dušková, Jaroslav Feyereisl, Luboš Minář, Milada Kohoutová
Title Detection of JCPyV microRNA in blood and urine samples of multiple sclerosis patients under natalizumab therapy
Journal Journal of NeuroVirology. 2015.
Authors Giovannelli I, Martelli F, Repice A, Massacesi L, Azzi A, Giannecchini S.
Title Circulating and urinary microRNA profile in focal segmental glomerulosclerosis: a pilot study
Journal European Journal of Clinical Investigation. 2015.
Authors Ramezani A, Devaney JM, Cohen S, Wing MR, Scott R, Knoblach S, Singhal R, Howard L, Kopp JB, Raj DS
Title A Simplified Method to Recover Urinary Vesicles for Clinical Applications, and Sample Banking
Journal Scientific Reports. 2014.
Authors Luca Musante, Dorota Tataruch, Dongfeng Gu, Alberto Benito-Martin, Giulio Calzaferri, Sinead Aherne & Harry Holthofer
Title Emerging technologies in extracellular vesicle-based molecular diagnostics
Journal Expert Review of Molecular Diagnostics. 2014.
Authors Shidong Jia, Davide Zocco, Michael L Samuels, Michael F Chou, Roger Chammas, Johan Skog, Natasa Zarovni, Fatemeh Momen-Heravi, and Winston Patrick Kuo
Title Overexpression of a DENND1A isoform produces a polycystic ovary syndrome theca phenotype.
Journal PNAS Journal. 2014.
Authors McAllister J,Modi B, Miller B, Biegler J, Bruggeman R, Legro R, and Strauss III J.
Title miR-23a is decreased during muscle atrophy by a mechanism that includes calcineurin signaling and exosome-mediated export.
Journal American Journal of Physiology. Cell Physiology. 2014.
Authors Hudson MB, Woodworth-Hobbs ME, Zheng B, Rahnert JA, Blount MA, Gooch JL, Searles CD, Price SR.
Title Characterization and deep sequencing analysis of exosomal and non-exosomal miRNA in human urine.
Journal Urine Exosome RNA Isolation Kit. .
Authors Cheng L, Sun X, Scicluna BJ, Coleman BM, Hill AF.