A rapid procedure for the isolation of exosomal RNA from urine sample

  • Isolation of exosomal RNA molecules from urine samples
  • Rapid and convenient spin-column protocol
  • Isolate inhibitor-free urinary microRNA for any application
  • Purification of exosomal proteins for western blot analysis
  • Purified exosomes can be analyzed using NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration
  • Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services

Related Services

NGS Service RNA Isolation Services

ItemsCat. #Size
Urine Exosome RNA Isolation Kit 47200 50 Preps

Urine Exosome RNA Isolation Kit

This kit provides a rapid spin column procedure for the isolation of exosomal RNA from urine samples.   Users can simultaneously concentrate and isolate high quality exosomal RNA, including microRNA, for use in sensitive downstream assays such as RT-PCR, qRT-PCR, NGS, microarrays and more.  The protocol can be completed in under 30 minutes.   Urine volumes of 1 to 10 mL can be processed easily and rapidly.  All sizes of RNA are recovered at an equal rate without the need for any phenol steps.  


Exosomes are 40 - 150 nm membrane vesicles, which are secreted by most cell types. Exosomes can be found in saliva, blood, urine, amniotic fluid and malignant ascite fluids, among other biological fluids. Evidence has been accumulating recently that these vesicles act as cellular messengers, conveying information to distant cells and tissues within the body. The exosomes contain cell-specific proteins, lipids and RNAs, which are transported to other cells, where they can alter function and/or physiology. These exosomes may play a functional role in mediating adaptive immune responses to infectious agents and tumours, tissue repair, neural communication and transfer of pathogenic proteins. Recent work has demonstrated the presence of distinct subsets of microRNAs within exosomes which depend upon the tumour cell type from which they are secreted. For this reason exosomal RNAs may serve as biomarkers for various diseases including cancer. As the RNA molecules encapsulated within exosomes are protected from degradation by RNAses they can be efficiently recovered from biological fluids, such as urine. 



Kit Specifications
Minimum Urine Input
1 mL
Maximum Urine Input
10 mL
Size of RNA Purified
Small exosomal RNA species
Time to Complete Purification
~ 50 minutes

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 1 year without showing any reduction in performance.

Title Urinary exosome-derived microRNAs reflecting the changes of renal function and histopathology in dogs
Journal Scientific Reports. 2017.
Authors Ichii O, Ohta H, Horino T, Nakamura T, Hosotani M, Mizoguchi T, Morishita K, Nakamura K, Hoshino Y, Takagi S, Sasaki N, Takiguchi M, Sato R, Oyamada K, Kon Y.
Title Comparative miRNA Analysis of Urine Extracellular Vesicles Isolated through Five Different Methods
Journal Cancers. 2016.
Authors Felix Royo, Izzuddin Diwan, Michael R. Tackett, Patricia Zuñiga, Pilar Sanchez-Mosquera, Ana Loizaga-Iriarte, Aitziber Ugalde-Olano, Isabel Lacasa, Amparo Perez, Miguel Unda, Arkaitz Carracedo, and Juan M. Falcon-Perez
Title High-throughput miRNA sequencing and identification of biomarkers for forensically relevant biological fluids
Journal Electrophoresis. 2016.
Authors Seashols-Williams S, Lewis C, Calloway C, Peace N, Harrison A, Hayes-Nash C, Fleming S, Wu Q, Zehner ZE
Title Urinary exosomal microRNA panel unravels novel biomarkers for diagnosis of type 2 Diabetic Kidney Disease
Journal Journal of Diabetes and its Complications. 2016.
Authors Eissa, S., Matboli, M., Aboushahba, R., Bekhet, M. M., & Soliman, Y
Title Vesicle-MaNiA: extracellular vesicles in liquid biopsy and cancer
Journal Current Opinion in Pharmacology. 2016.
Authors Torrano V, Royo F, Peinado H, Loizaga-Iriarte A, Unda M, Falcón-Perez JM, Carracedo A
Title Edible ginger-derived nanoparticles: A novel therapeutic approach for the prevention and treatment of inflammatory bowel disease and colitis-associated cancer
Journal Biomaterials. 2016.
Authors Zhang M, Viennois E, Prasad M, Zhang Y, Wang L, Zhang Z, Han MK, Xiao B, Xu C, Srinivasan S, Merlin D
Title Urinary extracellular vesicles for RNA extraction: optimization of a protocol devoid of prokaryote contamination
Journal Journal of Extracellular Vesicles. 2016.
Authors Tataruch-Weinert D, Musante L, Kretz O, Holthofer H
Title Investigation on torquetenovirus (TTV) microRNA transcriptome in vivo
Journal Virus Research. 2016.
Authors Vignolini, T., Macera, L., Antonelli, G., Pistello, M., Maggi, F., & Giannecchini, S
Title Polyomavirus JC microRNA expression after infection in vitro
Journal Virus Research. 2016.
Authors Irene Giovannellia, Valeria Clausia, Souichi Nukuzumab, Nunzia Della Malvaa, Daniele Nosia, Simone Giannecchini
Title Protein Complexes in Urine Interfere with Extracellular Vesicle Biomarker Studies
Journal Journal of Circulating Biomarkers. 2016.
Authors Magda Wachalska, Danijela Koppers-Lalic, Monique van Eijndhoven, Michiel Pegtel, Albert A. Geldof, Andrea D. Lipinska, R. Jeroen van Moorselaar and Irene V. Bijnsdorp
Title Different EV enrichment methods suitable for clinical settings yield different subpopulations of urinary extracellular vesicles from human samples
Journal Journal of Extracellular Vesicles. 2016.
Authors Royo F, Zuñiga-Garcia P, Sanchez-Mosquera P, Egia A, Perez A, Loizaga A, Arceo R, Lacasa I, Rabade A, Arrieta E, Bilbao R, Unda M4, Carracedo A, Falcon-Perez JM
Title Protocol for miRNA isolation from biofluids
Journal Analytical Biochemistry. 2016.
Authors Lekchnov EA, Zaporozhchenko IA, Morozkin ES, Bryzgunova OE, Vlassov VV, Laktionov PP
Title Exosomal MicroRNAs Are Diagnostic Biomarkers and Can Mediate Cell–Cell Communication in Renal Cell Carcinoma
Journal European Urology Focus. 2015.
Authors Henriett Butz, Roy Nofech-Mozes, Qiang Ding, Heba W.Z. Khella, Peter M. Szabó, Michael Jewett, Antonio Finelli, Jason Lee, Michael Ordon, Robert Stewart, Sergey Kreylov, George M. Yousef
Title Evaluation of optimal extracellular vesicle small RNA isolation and qRT-PCR normalisation for serum and urine samples.
Journal Journal of Immunological Methods. 2015.
Authors Crossland RE, Norden J, Bibby LA, Davis J, Dickinson AM
Title Supernatant versus exosomal urinary microRNAs. Two fractions with different outcomes in gynaecological cancers.
Journal Neoplasma. 2015.
Authors Zavesky L, Jandakova E, Turyna R, Langmeierova L, Weinberger V, Minar L
Title Exosomal lncRNA-p21 levels may help to distinguish prostate cancer from benign disease
Journal Frontiers in Genetics. 2015.
Authors Mustafa Isin, Ege Uysaler, Emre Ozgur, Hikmet Koseoglu, Oner Sanli, Omer Baris Yucel, Ugur Gezer and Nejat Dalay
Title Evaluation of Cell-Free Urine microRNAs Expression for the Use in Diagnosis of Ovarian and Endometrial Cancers. A Pilot Study
Journal Pathology & Oncology Research. 2015.
Authors Luděk Záveský, Eva Jandáková, Radovan Turyna, Lucie Langmeierová, Vít Weinberger, Lenka Záveská Drábková, Martina Hůlková, Aleš Hořínek, Daniela Dušková, Jaroslav Feyereisl, Luboš Minář, Milada Kohoutová
Title Detection of JCPyV microRNA in blood and urine samples of multiple sclerosis patients under natalizumab therapy
Journal Journal of NeuroVirology. 2015.
Authors Giovannelli I, Martelli F, Repice A, Massacesi L, Azzi A, Giannecchini S.
Title Circulating and urinary microRNA profile in focal segmental glomerulosclerosis: a pilot study
Journal European Journal of Clinical Investigation. 2015.
Authors Ramezani A, Devaney JM, Cohen S, Wing MR, Scott R, Knoblach S, Singhal R, Howard L, Kopp JB, Raj DS
Title Validation of Isolation Methodology and Endogenous Control Selection for qRT-PCR Assessment of Microrna Expression in Serum and Urine Exosomes
Journal Blood. 2014.
Authors Rachel E Crossland, Jean Norden, Louis Bibby, Joanna Davis, Anne M Dickinson
Title A Simplified Method to Recover Urinary Vesicles for Clinical Applications, and Sample Banking
Journal Scientific Reports. 2014.
Authors Luca Musante, Dorota Tataruch, Dongfeng Gu, Alberto Benito-Martin, Giulio Calzaferri, Sinead Aherne & Harry Holthofer
Title Emerging technologies in extracellular vesicle-based molecular diagnostics
Journal Expert Review of Molecular Diagnostics. 2014.
Authors Shidong Jia, Davide Zocco, Michael L Samuels, Michael F Chou, Roger Chammas, Johan Skog, Natasa Zarovni, Fatemeh Momen-Heravi, and Winston Patrick Kuo
Title Overexpression of a DENND1A isoform produces a polycystic ovary syndrome theca phenotype.
Journal PNAS Journal. 2014.
Authors McAllister J,Modi B, Miller B, Biegler J, Bruggeman R, Legro R, and Strauss III J.
Title miR-23a is decreased during muscle atrophy by a mechanism that includes calcineurin signaling and exosome-mediated export.
Journal American Journal of Physiology. Cell Physiology. 2014.
Authors Hudson MB, Woodworth-Hobbs ME, Zheng B, Rahnert JA, Blount MA, Gooch JL, Searles CD, Price SR.
Title Characterization and deep sequencing analysis of exosomal and non-exosomal miRNA in human urine.
Journal Kidney International. 2014.
Authors Cheng L, Sun X, Scicluna BJ, Coleman BM, Hill AF.