Rapid purification of microRNA from urine without phenol
|Urine microRNA Purification Kit||29000||25 preps|
Urine microRNA Purification Kit
Norgen’s Urine microRNA Purification Kit provides a rapid method for the isolation and purification of small RNA molecules (< 200 nt) from urine samples. These small RNAs include regulatory RNA molecules such as microRNA (miRNA) as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. Typically miRNAs are 20-25 nucleotides long, and regulate gene expression by binding to mRNA molecules and affecting their stability or translation. Several recent studies have shown that miRNA regulates cell growth and apoptosis. Furthermore, clinical and experimental analyses suggested that miRNAs may function as a novel class of oncogenes or tumor suppressor genes. MicroRNA expression profiles of different tumor types, relative to their normal tissues, have recently been shown to provide phenotypic signatures for particular cancer types. Unique patterns of aberrant miRNA expression may serve as molecular biomarkers for tumor diagnosis, prognosis of disease specific outcomes, and prediction of therapeutic responses.
Minimum Urine Input
|Maximum Urine Input||
|Size of RNA Purified||
< 200 nt
|Time to Complete Purification||
< 30 minutes
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
|Title||Feasibility of urinary microRNA detection in breast cancer patients and its potential as an innovative non-invasive biomarker.|
|Journal||BMC Cancer. 2015.|
|Authors||Thalia Erbes, Marc Hirschfeld, Gerta Rücker, Markus Jaeger, Jasmin Boas, Severine Iborra, Sebastian Mayer, Gerald Gitsch and Elmar Stickeler.|
|Title||Urinary microRNA-29 correlates with urinary albumin in type 2 diabetes mellitus.|
|Journal||Chinese Journal of Nephrology. 2014.|
|Authors||Meirong Z, Hui P, Wenbo Z, Cheng W, Jun Z, Xun L, Yuanqing L, Paudel S, Qianqian W, Tanqi L.|
|Title||Urinary miR-29 Correlates with Albuminuria and Carotid Intima-Media Thickness in Type 2 Diabetes Patients.|
|Journal||PLoS One. 2013.|
|Authors||Peng H, Zhong M, Zhao W, Wang C, Zhang J, Liu X, Li Y, Paudel S, Wang Q, Lou T.|
Copyright © 2015 - Norgen Biotek Corp.