Urine microRNA Purification Kit Rapid purification of microRNA from urine without phenol For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Urine microRNA Purification Kit Rapid purification of microRNA from urine without phenol Price USD$279.00 SKU 29000 Format Spin Column Size 25 Preps Quantity Overview Details Documentation Citations Overview Isolate high quality total RNA (including small RNA and microRNA) and all sizes of circulating and exosomal RNA, including microRNA from urine and cerebrospinal fluid (CSF) samples Small urine and CSF input ranging from as low as 0.5 mL to 1 mL No phenol extractions Very sensitive and linear down to a few cells without the need for carrier RNA Bind and elute all RNA irrespective of size or GC content, without bias Rapid and convenient spin column protocol Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix Norgen’s Urine microRNA Purification Kit provides a rapid method for the isolation and purification of small RNA molecules (All sizes, including < 200 nt) from urine samples. These small RNAs include regulatory RNA molecules such as microRNA (miRNA) as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. Typically miRNAs are 20-25 nucleotides long and regulate gene expression by binding to mRNA molecules and affecting their stability or translation. Several recent studies have shown that miRNA regulates cell growth and apoptosis. Furthermore, clinical and experimental analyses suggested that miRNAs may function as a novel class of oncogenes or tumor suppressor genes. MicroRNA expression profiles of different tumor types, relative to their normal tissues, have recently been shown to provide phenotypic signatures for particular cancer types. Unique patterns of aberrant miRNA expression may serve as molecular biomarkers for tumor diagnosis, prognosis of disease-specific outcomes, and prediction of therapeutic responses. Details Supporting Data Previous Figure 1. Isolation and Detection of microRNA from Urine Samples. Norgen's Urine microRNA Purification Kit was used to isolate microRNA from 8 different 1.5 mL urine samples. The purified microRNA was then used as the template in an RT-qPCR reaction to detect the human miR-21 gene. Five microlitres of the isolated RNA were used as the template in the RT step, and 5 µL from the RT step was used in the qPCR reaction. As it can be seen, the qPCR was able to successfully detect and amplify the miR-21 gene in all cases, indicating the high quality of the isolated urine microRNA. The black line in the graph above corresponds to the No Template Control. Next Click for expanded view Kit Specifications Volume of Urine Processed 0.5 - 1 mL Size of RNA Purified All sizes, including < 200 nt Time to Complete 10 Purifications < 30 minutes Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. Component Cat. 29000 (25 preps) Lysis Buffer A 2 x 20 mL Wash Solution A 18 mL Elution Solution A 6 mL Mini Spin Columns 25 Collection Tubes 25 Elution Tubes (1.7 mL) 25 Product Insert 1 Documentation PROTOCOLS PI29000-8 Urine microRNA Purification Kit Insert.pdf APPLICATION NOTES urine_microrna_purification_kit_effect_of_microrna_gc_content_on_rna_purification_efficiency_application_notes_229.pdf App-Note-100-Effects-of-microRNA-GC-Content-1.0.pdf SAFETY DATA SHEETS 29000_SDS.pdf POSTERS urine_microrna_purification_kit_differential_expression_of_micrornas_assayed_from_urine_of_patients_with_prostate_cancer_or_bph_posters_229.pdf Poster 46 - Effect of microRNA Content on RNA Purification Efficiency from Biological and Clinical Specimens using Various Isolation Procedures-compressed.pdf RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class How to Reduce False Negative SARS-CoV-2 Results with Silicon Carbide Citations Powered by Bioz See more details on Bioz Related Products Urine Total RNA Purification Maxi Kit (Slurry Format) Urine Cell-Free Circulating RNA Purification Mini Kit microRNA (cel-miR-39) Spike-In Kit
Urine microRNA Purification Kit Rapid purification of microRNA from urine without phenol For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Urine microRNA Purification Kit Rapid purification of microRNA from urine without phenol Price USD$279.00 SKU 29000 Format Spin Column Size 25 Preps Quantity