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Rapid purification of microRNA from urine without phenol
Urine microRNA Purification Kit
Norgen’s Urine microRNA Purification Kit provides a rapid method for the isolation and purification of small RNA molecules (All sizes, including < 200 nt) from urine samples. These small RNAs include regulatory RNA molecules such as microRNA (miRNA) as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. Typically miRNAs are 20-25 nucleotides long, and regulate gene expression by binding to mRNA molecules and affecting their stability or translation. Several recent studies have shown that miRNA regulates cell growth and apoptosis. Furthermore, clinical and experimental analyses suggested that miRNAs may function as a novel class of oncogenes or tumor suppressor genes. MicroRNA expression profiles of different tumor types, relative to their normal tissues, have recently been shown to provide phenotypic signatures for particular cancer types. Unique patterns of aberrant miRNA expression may serve as molecular biomarkers for tumor diagnosis, prognosis of disease specific outcomes, and prediction of therapeutic responses.
Kit Specifications
|
|
Volume of Urine Processed
|
0.5 - 1 mL
|
Size of RNA Purified | All sizes, including < 200 nt |
Time to Complete 10 Purifications |
< 30 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
Title | MicroRNA isolation and quantification in cerebrospinal fluid: A comparative methodical study |
Journal | PLOS | One. 2018. |
Authors | Alena Kopkova, Jiri Sana, Pavel Fadrus, Tana Machackova, Marek Vecera, Vaclav Vybihal, Jaroslav Juracek, Petra Vychytilova-Faltejskova, Martin Smrcka, Ondrej Slaby |
Title | Expression Levels of PIWI-interacting RNA, piR-823, Are Deregulated in Tumor Tissue, Blood Serum and Urine of Patients with Renal Cell Carcinoma |
Journal | Anticancer Research. 2016. |
Authors | Iliev R, Fedorko M, Machackova T, Mlcochova H, Svoboda M,, Pacik D, Dolezel J, Stanik M, Slaby O, |
Title | Comparison of MicroRNA Content in Plasma and Urine Indicates the Existence of a Transrenal Passage of Selected MicroRNAs |
Journal | Advances in Experimental Medicine and Biology. 2016. |
Authors | Pazourkova E, Pospisilova S, Svobodova I, Horinek A, Brisuda A, Soukup V, Hrbacek J, Capoun O, Mares J, Hanus T, Babjuk M, Korabecna M. |
Title | MicroRNAs in urine supernatant as potential non-invasive markers for bladder cancer detection |
Journal | Neoplasma. 2016. |
Authors | Pospisilova S, Pazourkova E, Horinek A, Brisuda A, Svobodova I, Soukup V, Hrbacek J, Capoun O, Hanus T, Mares J, Korabecna M, Babjuk M. |
Title | Biomarker Potential of Urine miR-451 at Different Stages of Diabetic Nephropathy |
Journal | Journal of Diabetes & Metabolism. 2016. |
Authors | Emel Isiktas Sayilar, Mustafa Gullulu, Ercan Tuncel, Hande Peynirci, Adem Alemdar, Berrin Tunca, Unal Egeli, Gulsah Cecener, Murat Bayindir and Gokhan Cosgun |
Title | Live Cell MicroRNA Imaging Using Exonuclease III-Aided Recycling Amplification Based on Aggregation-Induced Emission Luminogens |
Journal | ACS Appl. Mater. Interfaces. 2016. |
Authors | Min, X., Zhang, M., Huang, F., Lou, X., & Xia, F |
Title | Noninvasive urinary miRNA biomarkers for early detection of pancreatic adenocarcinoma |
Journal | American Journal of Cancer Research. 2016. |
Authors | Debernardi S, Massat NJ, Radon T1, Sangaralingam A, Banissi A, Ennis DP, Dowe T, Chelala C, Pereira SP, Kocher HM, Young BD, Bond-Smith G, Hutchins R, Crnogorac-Jurcevic T |
Title | MicroRNA molecular profiling from matched tumor and bio-fluids in bladder cancer |
Journal | Molecular Cancer. 2015. |
Authors | David A. Armstrong, Benjamin B. Green, John D. Seigne, Alan R. Schned and Carmen J. Marsit |
Title | MicroRNAs in urine are not biomarkers of multiple myeloma |
Journal | Journal of Negative Results in Biomedicine. 2015. |
Authors | Lenka Sedlaříková, Lenka Bešše, Soňa Novosadová, Veronika Kubaczková, Lenka Radová, Michal Staník, Marta Krejčí, Roman Hájek1 and Sabina Ševčíková |
Title | Feasibility of urinary microRNA detection in breast cancer patients and its potential as an innovative non-invasive biomarker. |
Journal | BMC Cancer. 2015. |
Authors | Thalia Erbes, Marc Hirschfeld, Gerta Rücker, Markus Jaeger, Jasmin Boas, Severine Iborra, Sebastian Mayer, Gerald Gitsch and Elmar Stickeler. |
Title | Urinary microRNA-29 correlates with urinary albumin in type 2 diabetes mellitus. |
Journal | Chinese Journal of Nephrology. 2014. |
Authors | Meirong Z, Hui P, Wenbo Z, Cheng W, Jun Z, Xun L, Yuanqing L, Paudel S, Qianqian W, Tanqi L. |
Title | Urinary miR-29 Correlates with Albuminuria and Carotid Intima-Media Thickness in Type 2 Diabetes Patients. |
Journal | PLoS One. 2013. |
Authors | Peng H, Zhong M, Zhao W, Wang C, Zhang J, Liu X, Li Y, Paudel S, Wang Q, Lou T. |
Urine microRNA Purification Kit
Norgen’s Urine microRNA Purification Kit provides a rapid method for the isolation and purification of small RNA molecules (All sizes, including < 200 nt) from urine samples. These small RNAs include regulatory RNA molecules such as microRNA (miRNA) as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. Typically miRNAs are 20-25 nucleotides long, and regulate gene expression by binding to mRNA molecules and affecting their stability or translation. Several recent studies have shown that miRNA regulates cell growth and apoptosis. Furthermore, clinical and experimental analyses suggested that miRNAs may function as a novel class of oncogenes or tumor suppressor genes. MicroRNA expression profiles of different tumor types, relative to their normal tissues, have recently been shown to provide phenotypic signatures for particular cancer types. Unique patterns of aberrant miRNA expression may serve as molecular biomarkers for tumor diagnosis, prognosis of disease specific outcomes, and prediction of therapeutic responses.
Kit Specifications
|
|
Volume of Urine Processed
|
0.5 - 1 mL
|
Size of RNA Purified | All sizes, including < 200 nt |
Time to Complete 10 Purifications |
< 30 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
Title | MicroRNA isolation and quantification in cerebrospinal fluid: A comparative methodical study |
Journal | PLOS | One. 2018. |
Authors | Alena Kopkova, Jiri Sana, Pavel Fadrus, Tana Machackova, Marek Vecera, Vaclav Vybihal, Jaroslav Juracek, Petra Vychytilova-Faltejskova, Martin Smrcka, Ondrej Slaby |
Title | Expression Levels of PIWI-interacting RNA, piR-823, Are Deregulated in Tumor Tissue, Blood Serum and Urine of Patients with Renal Cell Carcinoma |
Journal | Anticancer Research. 2016. |
Authors | Iliev R, Fedorko M, Machackova T, Mlcochova H, Svoboda M,, Pacik D, Dolezel J, Stanik M, Slaby O, |
Title | Comparison of MicroRNA Content in Plasma and Urine Indicates the Existence of a Transrenal Passage of Selected MicroRNAs |
Journal | Advances in Experimental Medicine and Biology. 2016. |
Authors | Pazourkova E, Pospisilova S, Svobodova I, Horinek A, Brisuda A, Soukup V, Hrbacek J, Capoun O, Mares J, Hanus T, Babjuk M, Korabecna M. |
Title | MicroRNAs in urine supernatant as potential non-invasive markers for bladder cancer detection |
Journal | Neoplasma. 2016. |
Authors | Pospisilova S, Pazourkova E, Horinek A, Brisuda A, Svobodova I, Soukup V, Hrbacek J, Capoun O, Hanus T, Mares J, Korabecna M, Babjuk M. |
Title | Biomarker Potential of Urine miR-451 at Different Stages of Diabetic Nephropathy |
Journal | Journal of Diabetes & Metabolism. 2016. |
Authors | Emel Isiktas Sayilar, Mustafa Gullulu, Ercan Tuncel, Hande Peynirci, Adem Alemdar, Berrin Tunca, Unal Egeli, Gulsah Cecener, Murat Bayindir and Gokhan Cosgun |
Title | Live Cell MicroRNA Imaging Using Exonuclease III-Aided Recycling Amplification Based on Aggregation-Induced Emission Luminogens |
Journal | ACS Appl. Mater. Interfaces. 2016. |
Authors | Min, X., Zhang, M., Huang, F., Lou, X., & Xia, F |
Title | Noninvasive urinary miRNA biomarkers for early detection of pancreatic adenocarcinoma |
Journal | American Journal of Cancer Research. 2016. |
Authors | Debernardi S, Massat NJ, Radon T1, Sangaralingam A, Banissi A, Ennis DP, Dowe T, Chelala C, Pereira SP, Kocher HM, Young BD, Bond-Smith G, Hutchins R, Crnogorac-Jurcevic T |
Title | MicroRNA molecular profiling from matched tumor and bio-fluids in bladder cancer |
Journal | Molecular Cancer. 2015. |
Authors | David A. Armstrong, Benjamin B. Green, John D. Seigne, Alan R. Schned and Carmen J. Marsit |
Title | MicroRNAs in urine are not biomarkers of multiple myeloma |
Journal | Journal of Negative Results in Biomedicine. 2015. |
Authors | Lenka Sedlaříková, Lenka Bešše, Soňa Novosadová, Veronika Kubaczková, Lenka Radová, Michal Staník, Marta Krejčí, Roman Hájek1 and Sabina Ševčíková |
Title | Feasibility of urinary microRNA detection in breast cancer patients and its potential as an innovative non-invasive biomarker. |
Journal | BMC Cancer. 2015. |
Authors | Thalia Erbes, Marc Hirschfeld, Gerta Rücker, Markus Jaeger, Jasmin Boas, Severine Iborra, Sebastian Mayer, Gerald Gitsch and Elmar Stickeler. |
Title | Urinary microRNA-29 correlates with urinary albumin in type 2 diabetes mellitus. |
Journal | Chinese Journal of Nephrology. 2014. |
Authors | Meirong Z, Hui P, Wenbo Z, Cheng W, Jun Z, Xun L, Yuanqing L, Paudel S, Qianqian W, Tanqi L. |
Title | Urinary miR-29 Correlates with Albuminuria and Carotid Intima-Media Thickness in Type 2 Diabetes Patients. |
Journal | PLoS One. 2013. |
Authors | Peng H, Zhong M, Zhao W, Wang C, Zhang J, Liu X, Li Y, Paudel S, Wang Q, Lou T. |
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