Urine microRNA Purification Kit

Cat. 29000
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Urine microRNA Purification Kit

Cat. 29000

Rapid purification of microRNA from urine without phenol

  • Isolate high quality total RNA (including small RNA and microRNA) and all sizes of circulating and exosomal RNA, including microRNA from urine and cerebrospinal fluid (CSF) samples
  • Small urine and CSF input ranging from as low as 0.5 mL to 1 mL
  • No phenol extractions
  • Very sensitive and linear down to a few cells without the need for carrier RNA
  • Bind and elute all RNA irrespective of size or GC content, without bias
  • Rapid and convenient spin column protocol
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

#TogetherWithNorgen

  • For every purchase of Urine kits during November, 10% of the proceeds will be donated to charity by Norgen.
  • Take a selfie wearing a Norgen moustache and share it using the hashtag #TogetherWithNorgen, and we will donate $10 in your name to charity.
  • Learn More by clicking here.

For research use only and NOT intended for in vitro diagnostics.

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Urine microRNA Purification Kit
(Cat. 29000)
25 preps

Kit Size

  • Isolate high quality total RNA (including small RNA and microRNA) and all sizes of circulating and exosomal RNA, including microRNA from urine and cerebrospinal fluid (CSF) samples
  • Small urine and CSF input ranging from as low as 0.5 mL to 1 mL
  • No phenol extractions
  • Very sensitive and linear down to a few cells without the need for carrier RNA
  • Bind and elute all RNA irrespective of size or GC content, without bias
  • Rapid and convenient spin column protocol
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

#TogetherWithNorgen

  • For every purchase of Urine kits during November, 10% of the proceeds will be donated to charity by Norgen.
  • Take a selfie wearing a Norgen moustache and share it using the hashtag #TogetherWithNorgen, and we will donate $10 in your name to charity.
  • Learn More by clicking here.

Urine microRNA Purification Kit

Norgen’s Urine microRNA Purification Kit provides a rapid method for the isolation and purification of small RNA molecules (All sizes, including < 200 nt) from urine samples. These small RNAs include regulatory RNA molecules such as microRNA (miRNA) as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. Typically miRNAs are 20-25 nucleotides long and regulate gene expression by binding to mRNA molecules and affecting their stability or translation. Several recent studies have shown that miRNA regulates cell growth and apoptosis. Furthermore, clinical and experimental analyses suggested that miRNAs may function as a novel class of oncogenes or tumor suppressor genes. MicroRNA expression profiles of different tumor types, relative to their normal tissues, have recently been shown to provide phenotypic signatures for particular cancer types. Unique patterns of aberrant miRNA expression may serve as molecular biomarkers for tumor diagnosis, prognosis of disease-specific outcomes, and prediction of therapeutic responses.

Kit Specifications

You have selected: Cat. 29000
Kit Specifications
Volume of Urine Processed
0.5 - 1 mL
Size of RNA Purified All sizes, including < 200 nt
Time to Complete 10 Purifications
< 30 minutes


Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Supporting Data

Click for expanded view

Component Cat. 29000 (25 preps)
Lysis Buffer A 2 x 20 mL
Wash Solution A 18 mL
Elution Solution A 6 mL
Mini Spin Columns 25
Collection Tubes 25
Elution Tubes (1.7 mL) 25
Product Insert 1

Title Urinary Levels of miR-491-5p and miR-592 as Potential Diagnostic Biomarkers in Female Aging Patients with OAB: A Pilot Study
Journal Metabolites. 2022.
Authors Philippe G. Cammisotto, Abubakr H. Mossa, Samer Shamout and Lysanne Campeau
Title Combined miRNA and SERS urine liquid biopsy for the point-of-care diagnosis and molecular stratification of bladder cancer
Journal Molecular Medicine. 2022.
Authors Tudor Moisoiu, Mihnea P. Dragomir, Stefania D. Iancu, Simon Schallenberg, Giovanni Birolo, Giulio Ferrero, Dan Burghelea, Andrei Stefancu, Ramona G. Cozan, Emilia Licarete, Alessandra Allione, Giuseppe Matullo, Gheorghita Iacob, Zoltán Bálint, Radu I. Badea, Alessio Naccarati, David Horst, Barbara Pardini, Nicolae Leopold & Florin Elec
Title The 8q24 region hosts miRNAs altered in biospecimens of colorectal and bladder cancer patients
Journal Cancer Medicine. 2022.
Authors Amedeo Gagliardi,Giulia Francescato,Giulio Ferrero,Giovanni Birolo,Sonia Tarallo,Antonio Francavilla,Giulia Piaggeschi,Carla Di Battista,Gaetano Gallo,Alberto Realis Luc,Carlotta Sacerdote,Giuseppe Matullo,Paolo Vineis,Alessio Naccarati,Barbara Pardini
Title Noninvasive Prognosis of Postmyocardial Infarction Using Urinary miRNA Ultratrace Detection Based on Single-Target DNA-Functionalized AuNPs
Journal ACS Appl. Mater. Interfaces. 2018.
Authors Qianlong Wang, Lancong Liu, Xiaoyi Chen, Tiantian Wang, Hua Zhou, Hui Huang, Linsen Qing, and Pei Luo
Title MicroRNA isolation and quantification in cerebrospinal fluid: A comparative methodical study
Journal PLOS | One. 2018.
Authors Alena Kopkova, Jiri Sana, Pavel Fadrus, Tana Machackova, Marek Vecera, Vaclav Vybihal, Jaroslav Juracek, Petra Vychytilova-Faltejskova, Martin Smrcka, Ondrej Slaby
Title Expression Levels of PIWI-interacting RNA, piR-823, Are Deregulated in Tumor Tissue, Blood Serum and Urine of Patients with Renal Cell Carcinoma
Journal Anticancer Research. 2016.
Authors Iliev R, Fedorko M, Machackova T, Mlcochova H, Svoboda M,, Pacik D, Dolezel J, Stanik M, Slaby O,
Title Comparison of MicroRNA Content in Plasma and Urine Indicates the Existence of a Transrenal Passage of Selected MicroRNAs
Journal Advances in Experimental Medicine and Biology. 2016.
Authors Pazourkova E, Pospisilova S, Svobodova I, Horinek A, Brisuda A, Soukup V, Hrbacek J, Capoun O, Mares J, Hanus T, Babjuk M, Korabecna M.
Title MicroRNAs in urine supernatant as potential non-invasive markers for bladder cancer detection
Journal Neoplasma. 2016.
Authors Pospisilova S, Pazourkova E, Horinek A, Brisuda A, Svobodova I, Soukup V, Hrbacek J, Capoun O, Hanus T, Mares J, Korabecna M, Babjuk M.
Title Biomarker Potential of Urine miR-451 at Different Stages of Diabetic Nephropathy
Journal Journal of Diabetes & Metabolism. 2016.
Authors Emel Isiktas Sayilar, Mustafa Gullulu, Ercan Tuncel, Hande Peynirci, Adem Alemdar, Berrin Tunca, Unal Egeli, Gulsah Cecener, Murat Bayindir and Gokhan Cosgun
Title Live Cell MicroRNA Imaging Using Exonuclease III-Aided Recycling Amplification Based on Aggregation-Induced Emission Luminogens
Journal ACS Appl. Mater. Interfaces. 2016.
Authors Min, X., Zhang, M., Huang, F., Lou, X., & Xia, F
Title Noninvasive urinary miRNA biomarkers for early detection of pancreatic adenocarcinoma
Journal American Journal of Cancer Research. 2016.
Authors Debernardi S, Massat NJ, Radon T1, Sangaralingam A, Banissi A, Ennis DP, Dowe T, Chelala C, Pereira SP, Kocher HM, Young BD, Bond-Smith G, Hutchins R, Crnogorac-Jurcevic T
Title MicroRNA molecular profiling from matched tumor and bio-fluids in bladder cancer
Journal Molecular Cancer. 2015.
Authors David A. Armstrong, Benjamin B. Green, John D. Seigne, Alan R. Schned and Carmen J. Marsit
Title MicroRNAs in urine are not biomarkers of multiple myeloma
Journal Journal of Negative Results in Biomedicine. 2015.
Authors Lenka Sedlaříková, Lenka Bešše, Soňa Novosadová, Veronika Kubaczková, Lenka Radová, Michal Staník, Marta Krejčí, Roman Hájek1 and Sabina Ševčíková
Title Feasibility of urinary microRNA detection in breast cancer patients and its potential as an innovative non-invasive biomarker.
Journal BMC Cancer. 2015.
Authors Thalia Erbes, Marc Hirschfeld, Gerta Rücker, Markus Jaeger, Jasmin Boas, Severine Iborra, Sebastian Mayer, Gerald Gitsch and Elmar Stickeler.
Title MicroRNA-21-5p upregulation in urine samples serves as novel biomarkers for early stage renal cell carcinoma patients diagnosis
Journal Cancer Res. 2014.
Authors Wei Meng, Hansjuerg Alder, Kirsteen Maclean, Simon Kirste, Petra Stegmaier, Anca Grosu, Arnab Chakravarti, Tim Lautenschlaeger
Title Urinary microRNA-29 correlates with urinary albumin in type 2 diabetes mellitus.
Journal Chinese Journal of Nephrology. 2014.
Authors Meirong Z, Hui P, Wenbo Z, Cheng W, Jun Z, Xun L, Yuanqing L, Paudel S, Qianqian W, Tanqi L.
Title Urinary miR-29 Correlates with Albuminuria and Carotid Intima-Media Thickness in Type 2 Diabetes Patients.
Journal PLoS One. 2013.
Authors Peng H, Zhong M, Zhao W, Wang C, Zhang J, Liu X, Li Y, Paudel S, Wang Q, Lou T.

Urine microRNA Purification Kit

Norgen’s Urine microRNA Purification Kit provides a rapid method for the isolation and purification of small RNA molecules (All sizes, including < 200 nt) from urine samples. These small RNAs include regulatory RNA molecules such as microRNA (miRNA) as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. Typically miRNAs are 20-25 nucleotides long and regulate gene expression by binding to mRNA molecules and affecting their stability or translation. Several recent studies have shown that miRNA regulates cell growth and apoptosis. Furthermore, clinical and experimental analyses suggested that miRNAs may function as a novel class of oncogenes or tumor suppressor genes. MicroRNA expression profiles of different tumor types, relative to their normal tissues, have recently been shown to provide phenotypic signatures for particular cancer types. Unique patterns of aberrant miRNA expression may serve as molecular biomarkers for tumor diagnosis, prognosis of disease-specific outcomes, and prediction of therapeutic responses.

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 29000
Kit Specifications
Volume of Urine Processed
0.5 - 1 mL
Size of RNA Purified All sizes, including < 200 nt
Time to Complete 10 Purifications
< 30 minutes


Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component Cat. 29000 (25 preps)
Lysis Buffer A 2 x 20 mL
Wash Solution A 18 mL
Elution Solution A 6 mL
Mini Spin Columns 25
Collection Tubes 25
Elution Tubes (1.7 mL) 25
Product Insert 1

Title Urinary Levels of miR-491-5p and miR-592 as Potential Diagnostic Biomarkers in Female Aging Patients with OAB: A Pilot Study
Journal Metabolites. 2022.
Authors Philippe G. Cammisotto, Abubakr H. Mossa, Samer Shamout and Lysanne Campeau
Title Combined miRNA and SERS urine liquid biopsy for the point-of-care diagnosis and molecular stratification of bladder cancer
Journal Molecular Medicine. 2022.
Authors Tudor Moisoiu, Mihnea P. Dragomir, Stefania D. Iancu, Simon Schallenberg, Giovanni Birolo, Giulio Ferrero, Dan Burghelea, Andrei Stefancu, Ramona G. Cozan, Emilia Licarete, Alessandra Allione, Giuseppe Matullo, Gheorghita Iacob, Zoltán Bálint, Radu I. Badea, Alessio Naccarati, David Horst, Barbara Pardini, Nicolae Leopold & Florin Elec
Title The 8q24 region hosts miRNAs altered in biospecimens of colorectal and bladder cancer patients
Journal Cancer Medicine. 2022.
Authors Amedeo Gagliardi,Giulia Francescato,Giulio Ferrero,Giovanni Birolo,Sonia Tarallo,Antonio Francavilla,Giulia Piaggeschi,Carla Di Battista,Gaetano Gallo,Alberto Realis Luc,Carlotta Sacerdote,Giuseppe Matullo,Paolo Vineis,Alessio Naccarati,Barbara Pardini
Title Noninvasive Prognosis of Postmyocardial Infarction Using Urinary miRNA Ultratrace Detection Based on Single-Target DNA-Functionalized AuNPs
Journal ACS Appl. Mater. Interfaces. 2018.
Authors Qianlong Wang, Lancong Liu, Xiaoyi Chen, Tiantian Wang, Hua Zhou, Hui Huang, Linsen Qing, and Pei Luo
Title MicroRNA isolation and quantification in cerebrospinal fluid: A comparative methodical study
Journal PLOS | One. 2018.
Authors Alena Kopkova, Jiri Sana, Pavel Fadrus, Tana Machackova, Marek Vecera, Vaclav Vybihal, Jaroslav Juracek, Petra Vychytilova-Faltejskova, Martin Smrcka, Ondrej Slaby
Title Expression Levels of PIWI-interacting RNA, piR-823, Are Deregulated in Tumor Tissue, Blood Serum and Urine of Patients with Renal Cell Carcinoma
Journal Anticancer Research. 2016.
Authors Iliev R, Fedorko M, Machackova T, Mlcochova H, Svoboda M,, Pacik D, Dolezel J, Stanik M, Slaby O,
Title Comparison of MicroRNA Content in Plasma and Urine Indicates the Existence of a Transrenal Passage of Selected MicroRNAs
Journal Advances in Experimental Medicine and Biology. 2016.
Authors Pazourkova E, Pospisilova S, Svobodova I, Horinek A, Brisuda A, Soukup V, Hrbacek J, Capoun O, Mares J, Hanus T, Babjuk M, Korabecna M.
Title MicroRNAs in urine supernatant as potential non-invasive markers for bladder cancer detection
Journal Neoplasma. 2016.
Authors Pospisilova S, Pazourkova E, Horinek A, Brisuda A, Svobodova I, Soukup V, Hrbacek J, Capoun O, Hanus T, Mares J, Korabecna M, Babjuk M.
Title Biomarker Potential of Urine miR-451 at Different Stages of Diabetic Nephropathy
Journal Journal of Diabetes & Metabolism. 2016.
Authors Emel Isiktas Sayilar, Mustafa Gullulu, Ercan Tuncel, Hande Peynirci, Adem Alemdar, Berrin Tunca, Unal Egeli, Gulsah Cecener, Murat Bayindir and Gokhan Cosgun
Title Live Cell MicroRNA Imaging Using Exonuclease III-Aided Recycling Amplification Based on Aggregation-Induced Emission Luminogens
Journal ACS Appl. Mater. Interfaces. 2016.
Authors Min, X., Zhang, M., Huang, F., Lou, X., & Xia, F
Title Noninvasive urinary miRNA biomarkers for early detection of pancreatic adenocarcinoma
Journal American Journal of Cancer Research. 2016.
Authors Debernardi S, Massat NJ, Radon T1, Sangaralingam A, Banissi A, Ennis DP, Dowe T, Chelala C, Pereira SP, Kocher HM, Young BD, Bond-Smith G, Hutchins R, Crnogorac-Jurcevic T
Title MicroRNA molecular profiling from matched tumor and bio-fluids in bladder cancer
Journal Molecular Cancer. 2015.
Authors David A. Armstrong, Benjamin B. Green, John D. Seigne, Alan R. Schned and Carmen J. Marsit
Title MicroRNAs in urine are not biomarkers of multiple myeloma
Journal Journal of Negative Results in Biomedicine. 2015.
Authors Lenka Sedlaříková, Lenka Bešše, Soňa Novosadová, Veronika Kubaczková, Lenka Radová, Michal Staník, Marta Krejčí, Roman Hájek1 and Sabina Ševčíková
Title Feasibility of urinary microRNA detection in breast cancer patients and its potential as an innovative non-invasive biomarker.
Journal BMC Cancer. 2015.
Authors Thalia Erbes, Marc Hirschfeld, Gerta Rücker, Markus Jaeger, Jasmin Boas, Severine Iborra, Sebastian Mayer, Gerald Gitsch and Elmar Stickeler.
Title MicroRNA-21-5p upregulation in urine samples serves as novel biomarkers for early stage renal cell carcinoma patients diagnosis
Journal Cancer Res. 2014.
Authors Wei Meng, Hansjuerg Alder, Kirsteen Maclean, Simon Kirste, Petra Stegmaier, Anca Grosu, Arnab Chakravarti, Tim Lautenschlaeger
Title Urinary microRNA-29 correlates with urinary albumin in type 2 diabetes mellitus.
Journal Chinese Journal of Nephrology. 2014.
Authors Meirong Z, Hui P, Wenbo Z, Cheng W, Jun Z, Xun L, Yuanqing L, Paudel S, Qianqian W, Tanqi L.
Title Urinary miR-29 Correlates with Albuminuria and Carotid Intima-Media Thickness in Type 2 Diabetes Patients.
Journal PLoS One. 2013.
Authors Peng H, Zhong M, Zhao W, Wang C, Zhang J, Liu X, Li Y, Paudel S, Wang Q, Lou T.

 


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