Rapid purification of microRNA from urine without phenol

  • Isolate high quality total RNA and all sizes of circulating and exosomal RNA, including microRNA from urine and csf samples
  • Small urine and csf input ranging from as low as 0.5 mL to 1 mL
  • No phenol extractions
  • Very sensitive and linear down to a few cells without the need for carrier RNA
  • Bind and elute all RNA irrespective of size or GC content, without bias
  • Rapid and convenient spin-column protocol

ItemsCat. #Size
Urine microRNA Purification Kit 29000 25 preps

Urine microRNA Purification Kit

Norgen’s Urine microRNA Purification Kit provides a rapid method for the isolation and purification of small RNA molecules (< 200 nt) from urine samples. These small RNAs include regulatory RNA molecules such as microRNA (miRNA) as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. Typically miRNAs are 20-25 nucleotides long, and regulate gene expression by binding to mRNA molecules and affecting their stability or translation. Several recent studies have shown that miRNA regulates cell growth and apoptosis. Furthermore, clinical and experimental analyses suggested that miRNAs may function as a novel class of oncogenes or tumor suppressor genes. MicroRNA expression profiles of different tumor types, relative to their normal tissues, have recently been shown to provide phenotypic signatures for particular cancer types. Unique patterns of aberrant miRNA expression may serve as molecular biomarkers for tumor diagnosis, prognosis of disease specific outcomes, and prediction of therapeutic responses.

Kit Specifications
Volume of Urine Processed
0.5 - 1 mL
Size of RNA Purified
< 200 nt
Time to Complete 10 Purifications
< 30 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.

Title MicroRNA isolation and quantification in cerebrospinal fluid: A comparative methodical study
Journal PLOS | One. 2018.
Authors Alena Kopkova, Jiri Sana, Pavel Fadrus, Tana Machackova, Marek Vecera, Vaclav Vybihal, Jaroslav Juracek, Petra Vychytilova-Faltejskova, Martin Smrcka, Ondrej Slaby
Title Expression Levels of PIWI-interacting RNA, piR-823, Are Deregulated in Tumor Tissue, Blood Serum and Urine of Patients with Renal Cell Carcinoma
Journal Anticancer Research. 2016.
Authors Iliev R, Fedorko M, Machackova T, Mlcochova H, Svoboda M,, Pacik D, Dolezel J, Stanik M, Slaby O,
Title Comparison of MicroRNA Content in Plasma and Urine Indicates the Existence of a Transrenal Passage of Selected MicroRNAs
Journal Advances in Experimental Medicine and Biology. 2016.
Authors Pazourkova E, Pospisilova S, Svobodova I, Horinek A, Brisuda A, Soukup V, Hrbacek J, Capoun O, Mares J, Hanus T, Babjuk M, Korabecna M.
Title MicroRNAs in urine supernatant as potential non-invasive markers for bladder cancer detection
Journal Neoplasma. 2016.
Authors Pospisilova S, Pazourkova E, Horinek A, Brisuda A, Svobodova I, Soukup V, Hrbacek J, Capoun O, Hanus T, Mares J, Korabecna M, Babjuk M.
Title Biomarker Potential of Urine miR-451 at Different Stages of Diabetic Nephropathy
Journal Journal of Diabetes & Metabolism. 2016.
Authors Emel Isiktas Sayilar, Mustafa Gullulu, Ercan Tuncel, Hande Peynirci, Adem Alemdar, Berrin Tunca, Unal Egeli, Gulsah Cecener, Murat Bayindir and Gokhan Cosgun
Title Live Cell MicroRNA Imaging Using Exonuclease III-Aided Recycling Amplification Based on Aggregation-Induced Emission Luminogens
Journal ACS Appl. Mater. Interfaces. 2016.
Authors Min, X., Zhang, M., Huang, F., Lou, X., & Xia, F
Title Noninvasive urinary miRNA biomarkers for early detection of pancreatic adenocarcinoma
Journal American Journal of Cancer Research. 2016.
Authors Debernardi S, Massat NJ, Radon T1, Sangaralingam A, Banissi A, Ennis DP, Dowe T, Chelala C, Pereira SP, Kocher HM, Young BD, Bond-Smith G, Hutchins R, Crnogorac-Jurcevic T
Title MicroRNA molecular profiling from matched tumor and bio-fluids in bladder cancer
Journal Molecular Cancer. 2015.
Authors David A. Armstrong, Benjamin B. Green, John D. Seigne, Alan R. Schned and Carmen J. Marsit
Title MicroRNAs in urine are not biomarkers of multiple myeloma
Journal Journal of Negative Results in Biomedicine. 2015.
Authors Lenka Sedlaříková, Lenka Bešše, Soňa Novosadová, Veronika Kubaczková, Lenka Radová, Michal Staník, Marta Krejčí, Roman Hájek1 and Sabina Ševčíková
Title Feasibility of urinary microRNA detection in breast cancer patients and its potential as an innovative non-invasive biomarker.
Journal BMC Cancer. 2015.
Authors Thalia Erbes, Marc Hirschfeld, Gerta Rücker, Markus Jaeger, Jasmin Boas, Severine Iborra, Sebastian Mayer, Gerald Gitsch and Elmar Stickeler.
Title Urinary microRNA-29 correlates with urinary albumin in type 2 diabetes mellitus.
Journal Chinese Journal of Nephrology. 2014.
Authors Meirong Z, Hui P, Wenbo Z, Cheng W, Jun Z, Xun L, Yuanqing L, Paudel S, Qianqian W, Tanqi L.
Title Urinary miR-29 Correlates with Albuminuria and Carotid Intima-Media Thickness in Type 2 Diabetes Patients.
Journal PLoS One. 2013.
Authors Peng H, Zhong M, Zhao W, Wang C, Zhang J, Liu X, Li Y, Paudel S, Wang Q, Lou T.