Urine microRNA Purification Kit (Cat. 29000)
Urine microRNA Purification Kit
![Urine microRNA Purification Kit](https://norgenbiotek.com/sites/default/files/styles/original/public/29000-Reflection-web.jpg?itok=t_JNEkMN" "Urine microRNA Purification Kit")
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Urine microRNA Purification Kit
Rapid purification of microRNA from urine without phenol
- Isolate high quality total RNA (including small RNA and microRNA) and all sizes of circulating and exosomal RNA, including microRNA from urine and cerebrospinal fluid (CSF) samples
- Small urine and CSF input ranging from as low as 0.5 mL to 1 mL
- No phenol extractions
- Very sensitive and linear down to a few cells without the need for carrier RNA
- Bind and elute all RNA irrespective of size or GC content, without bias
- Rapid and convenient spin column protocol
- Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
#TogetherWithNorgen
- For every purchase of Urine kits during November, 10% of the proceeds will be donated to charity by Norgen.
- Take a selfie wearing a Norgen moustache and share it using the hashtag #TogetherWithNorgen, and we will donate $10 in your name to charity.
- Learn More by clicking here.
For research use only and NOT intended for in vitro diagnostics.
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(Cat. 29000) 25 preps
Kit Size
- Isolate high quality total RNA (including small RNA and microRNA) and all sizes of circulating and exosomal RNA, including microRNA from urine and cerebrospinal fluid (CSF) samples
- Small urine and CSF input ranging from as low as 0.5 mL to 1 mL
- No phenol extractions
- Very sensitive and linear down to a few cells without the need for carrier RNA
- Bind and elute all RNA irrespective of size or GC content, without bias
- Rapid and convenient spin column protocol
- Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
#TogetherWithNorgen
- For every purchase of Urine kits during November, 10% of the proceeds will be donated to charity by Norgen.
- Take a selfie wearing a Norgen moustache and share it using the hashtag #TogetherWithNorgen, and we will donate $10 in your name to charity.
- Learn More by clicking here.
Urine microRNA Purification Kit
Norgen’s Urine microRNA Purification Kit provides a rapid method for the isolation and purification of small RNA molecules (All sizes, including < 200 nt) from urine samples. These small RNAs include regulatory RNA molecules such as microRNA (miRNA) as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. Typically miRNAs are 20-25 nucleotides long and regulate gene expression by binding to mRNA molecules and affecting their stability or translation. Several recent studies have shown that miRNA regulates cell growth and apoptosis. Furthermore, clinical and experimental analyses suggested that miRNAs may function as a novel class of oncogenes or tumor suppressor genes. MicroRNA expression profiles of different tumor types, relative to their normal tissues, have recently been shown to provide phenotypic signatures for particular cancer types. Unique patterns of aberrant miRNA expression may serve as molecular biomarkers for tumor diagnosis, prognosis of disease-specific outcomes, and prediction of therapeutic responses.
Kit Specifications
|
Kit Specifications
|
|
|
Volume of Urine Processed
|
0.5 - 1 mL
|
| Size of RNA Purified | All sizes, including < 200 nt |
| Time to Complete 10 Purifications |
< 30 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Supporting Data
Figure 1. Isolation and Detection of microRNA from Urine Samples. Norgen's Urine microRNA Purification Kit was used to isolate microRNA from 8 different 1.5 mL urine samples. The purified microRNA was then used as the template in an RT-qPCR reaction to detect the human miR-21 gene. Five microlitres of the isolated RNA were used as the template in the RT step, and 5 µL from the RT step was used in the qPCR reaction. As it can be seen, the qPCR was able to successfully detect and amplify the miR-21 gene in all cases, indicating the high quality of the isolated urine microRNA. The black line in the graph above corresponds to the No Template Control.
| Component | Cat. 29000 (25 preps) |
|---|---|
| Lysis Buffer A | 2 x 20 mL |
| Wash Solution A | 18 mL |
| Elution Solution A | 6 mL |
| Mini Spin Columns | 25 |
| Collection Tubes | 25 |
| Elution Tubes (1.7 mL) | 25 |
| Product Insert | 1 |
| Title | Urinary Levels of miR-491-5p and miR-592 as Potential Diagnostic Biomarkers in Female Aging Patients with OAB: A Pilot Study |
| Journal | Metabolites. 2022. |
| Authors | Philippe G. Cammisotto, Abubakr H. Mossa, Samer Shamout and Lysanne Campeau |
| Title | Combined miRNA and SERS urine liquid biopsy for the point-of-care diagnosis and molecular stratification of bladder cancer |
| Journal | Molecular Medicine. 2022. |
| Authors | Tudor Moisoiu, Mihnea P. Dragomir, Stefania D. Iancu, Simon Schallenberg, Giovanni Birolo, Giulio Ferrero, Dan Burghelea, Andrei Stefancu, Ramona G. Cozan, Emilia Licarete, Alessandra Allione, Giuseppe Matullo, Gheorghita Iacob, Zoltán Bálint, Radu I. Badea, Alessio Naccarati, David Horst, Barbara Pardini, Nicolae Leopold & Florin Elec |
| Title | The 8q24 region hosts miRNAs altered in biospecimens of colorectal and bladder cancer patients |
| Journal | Cancer Medicine. 2022. |
| Authors | Amedeo Gagliardi,Giulia Francescato,Giulio Ferrero,Giovanni Birolo,Sonia Tarallo,Antonio Francavilla,Giulia Piaggeschi,Carla Di Battista,Gaetano Gallo,Alberto Realis Luc,Carlotta Sacerdote,Giuseppe Matullo,Paolo Vineis,Alessio Naccarati,Barbara Pardini |
| Title | Noninvasive Prognosis of Postmyocardial Infarction Using Urinary miRNA Ultratrace Detection Based on Single-Target DNA-Functionalized AuNPs |
| Journal | ACS Appl. Mater. Interfaces. 2018. |
| Authors | Qianlong Wang, Lancong Liu, Xiaoyi Chen, Tiantian Wang, Hua Zhou, Hui Huang, Linsen Qing, and Pei Luo |
| Title | MicroRNA isolation and quantification in cerebrospinal fluid: A comparative methodical study |
| Journal | PLOS | One. 2018. |
| Authors | Alena Kopkova, Jiri Sana, Pavel Fadrus, Tana Machackova, Marek Vecera, Vaclav Vybihal, Jaroslav Juracek, Petra Vychytilova-Faltejskova, Martin Smrcka, Ondrej Slaby |
| Title | Expression Levels of PIWI-interacting RNA, piR-823, Are Deregulated in Tumor Tissue, Blood Serum and Urine of Patients with Renal Cell Carcinoma |
| Journal | Anticancer Research. 2016. |
| Authors | Iliev R, Fedorko M, Machackova T, Mlcochova H, Svoboda M,, Pacik D, Dolezel J, Stanik M, Slaby O, |
| Title | Comparison of MicroRNA Content in Plasma and Urine Indicates the Existence of a Transrenal Passage of Selected MicroRNAs |
| Journal | Advances in Experimental Medicine and Biology. 2016. |
| Authors | Pazourkova E, Pospisilova S, Svobodova I, Horinek A, Brisuda A, Soukup V, Hrbacek J, Capoun O, Mares J, Hanus T, Babjuk M, Korabecna M. |
| Title | MicroRNAs in urine supernatant as potential non-invasive markers for bladder cancer detection |
| Journal | Neoplasma. 2016. |
| Authors | Pospisilova S, Pazourkova E, Horinek A, Brisuda A, Svobodova I, Soukup V, Hrbacek J, Capoun O, Hanus T, Mares J, Korabecna M, Babjuk M. |
| Title | Biomarker Potential of Urine miR-451 at Different Stages of Diabetic Nephropathy |
| Journal | Journal of Diabetes & Metabolism. 2016. |
| Authors | Emel Isiktas Sayilar, Mustafa Gullulu, Ercan Tuncel, Hande Peynirci, Adem Alemdar, Berrin Tunca, Unal Egeli, Gulsah Cecener, Murat Bayindir and Gokhan Cosgun |
| Title | Live Cell MicroRNA Imaging Using Exonuclease III-Aided Recycling Amplification Based on Aggregation-Induced Emission Luminogens |
| Journal | ACS Appl. Mater. Interfaces. 2016. |
| Authors | Min, X., Zhang, M., Huang, F., Lou, X., & Xia, F |
| Title | Noninvasive urinary miRNA biomarkers for early detection of pancreatic adenocarcinoma |
| Journal | American Journal of Cancer Research. 2016. |
| Authors | Debernardi S, Massat NJ, Radon T1, Sangaralingam A, Banissi A, Ennis DP, Dowe T, Chelala C, Pereira SP, Kocher HM, Young BD, Bond-Smith G, Hutchins R, Crnogorac-Jurcevic T |
| Title | MicroRNA molecular profiling from matched tumor and bio-fluids in bladder cancer |
| Journal | Molecular Cancer. 2015. |
| Authors | David A. Armstrong, Benjamin B. Green, John D. Seigne, Alan R. Schned and Carmen J. Marsit |
| Title | MicroRNAs in urine are not biomarkers of multiple myeloma |
| Journal | Journal of Negative Results in Biomedicine. 2015. |
| Authors | Lenka Sedlaříková, Lenka Bešše, Soňa Novosadová, Veronika Kubaczková, Lenka Radová, Michal Staník, Marta Krejčí, Roman Hájek1 and Sabina Ševčíková |
| Title | Feasibility of urinary microRNA detection in breast cancer patients and its potential as an innovative non-invasive biomarker. |
| Journal | BMC Cancer. 2015. |
| Authors | Thalia Erbes, Marc Hirschfeld, Gerta Rücker, Markus Jaeger, Jasmin Boas, Severine Iborra, Sebastian Mayer, Gerald Gitsch and Elmar Stickeler. |
| Title | MicroRNA-21-5p upregulation in urine samples serves as novel biomarkers for early stage renal cell carcinoma patients diagnosis |
| Journal | Cancer Res. 2014. |
| Authors | Wei Meng, Hansjuerg Alder, Kirsteen Maclean, Simon Kirste, Petra Stegmaier, Anca Grosu, Arnab Chakravarti, Tim Lautenschlaeger |
| Title | Urinary microRNA-29 correlates with urinary albumin in type 2 diabetes mellitus. |
| Journal | Chinese Journal of Nephrology. 2014. |
| Authors | Meirong Z, Hui P, Wenbo Z, Cheng W, Jun Z, Xun L, Yuanqing L, Paudel S, Qianqian W, Tanqi L. |
| Title | Urinary miR-29 Correlates with Albuminuria and Carotid Intima-Media Thickness in Type 2 Diabetes Patients. |
| Journal | PLoS One. 2013. |
| Authors | Peng H, Zhong M, Zhao W, Wang C, Zhang J, Liu X, Li Y, Paudel S, Wang Q, Lou T. |
Urine microRNA Purification Kit
Norgen’s Urine microRNA Purification Kit provides a rapid method for the isolation and purification of small RNA molecules (All sizes, including < 200 nt) from urine samples. These small RNAs include regulatory RNA molecules such as microRNA (miRNA) as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. Typically miRNAs are 20-25 nucleotides long and regulate gene expression by binding to mRNA molecules and affecting their stability or translation. Several recent studies have shown that miRNA regulates cell growth and apoptosis. Furthermore, clinical and experimental analyses suggested that miRNAs may function as a novel class of oncogenes or tumor suppressor genes. MicroRNA expression profiles of different tumor types, relative to their normal tissues, have recently been shown to provide phenotypic signatures for particular cancer types. Unique patterns of aberrant miRNA expression may serve as molecular biomarkers for tumor diagnosis, prognosis of disease-specific outcomes, and prediction of therapeutic responses.
Supporting Data
Figure 1. Isolation and Detection of microRNA from Urine Samples. Norgen's Urine microRNA Purification Kit was used to isolate microRNA from 8 different 1.5 mL urine samples. The purified microRNA was then used as the template in an RT-qPCR reaction to detect the human miR-21 gene. Five microlitres of the isolated RNA were used as the template in the RT step, and 5 µL from the RT step was used in the qPCR reaction. As it can be seen, the qPCR was able to successfully detect and amplify the miR-21 gene in all cases, indicating the high quality of the isolated urine microRNA. The black line in the graph above corresponds to the No Template Control.
Kit Specifications
|
Kit Specifications
|
|
|
Volume of Urine Processed
|
0.5 - 1 mL
|
| Size of RNA Purified | All sizes, including < 200 nt |
| Time to Complete 10 Purifications |
< 30 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 29000 (25 preps) |
|---|---|
| Lysis Buffer A | 2 x 20 mL |
| Wash Solution A | 18 mL |
| Elution Solution A | 6 mL |
| Mini Spin Columns | 25 |
| Collection Tubes | 25 |
| Elution Tubes (1.7 mL) | 25 |
| Product Insert | 1 |
| Title | Urinary Levels of miR-491-5p and miR-592 as Potential Diagnostic Biomarkers in Female Aging Patients with OAB: A Pilot Study |
| Journal | Metabolites. 2022. |
| Authors | Philippe G. Cammisotto, Abubakr H. Mossa, Samer Shamout and Lysanne Campeau |
| Title | Combined miRNA and SERS urine liquid biopsy for the point-of-care diagnosis and molecular stratification of bladder cancer |
| Journal | Molecular Medicine. 2022. |
| Authors | Tudor Moisoiu, Mihnea P. Dragomir, Stefania D. Iancu, Simon Schallenberg, Giovanni Birolo, Giulio Ferrero, Dan Burghelea, Andrei Stefancu, Ramona G. Cozan, Emilia Licarete, Alessandra Allione, Giuseppe Matullo, Gheorghita Iacob, Zoltán Bálint, Radu I. Badea, Alessio Naccarati, David Horst, Barbara Pardini, Nicolae Leopold & Florin Elec |
| Title | The 8q24 region hosts miRNAs altered in biospecimens of colorectal and bladder cancer patients |
| Journal | Cancer Medicine. 2022. |
| Authors | Amedeo Gagliardi,Giulia Francescato,Giulio Ferrero,Giovanni Birolo,Sonia Tarallo,Antonio Francavilla,Giulia Piaggeschi,Carla Di Battista,Gaetano Gallo,Alberto Realis Luc,Carlotta Sacerdote,Giuseppe Matullo,Paolo Vineis,Alessio Naccarati,Barbara Pardini |
| Title | Noninvasive Prognosis of Postmyocardial Infarction Using Urinary miRNA Ultratrace Detection Based on Single-Target DNA-Functionalized AuNPs |
| Journal | ACS Appl. Mater. Interfaces. 2018. |
| Authors | Qianlong Wang, Lancong Liu, Xiaoyi Chen, Tiantian Wang, Hua Zhou, Hui Huang, Linsen Qing, and Pei Luo |
| Title | MicroRNA isolation and quantification in cerebrospinal fluid: A comparative methodical study |
| Journal | PLOS | One. 2018. |
| Authors | Alena Kopkova, Jiri Sana, Pavel Fadrus, Tana Machackova, Marek Vecera, Vaclav Vybihal, Jaroslav Juracek, Petra Vychytilova-Faltejskova, Martin Smrcka, Ondrej Slaby |
| Title | Expression Levels of PIWI-interacting RNA, piR-823, Are Deregulated in Tumor Tissue, Blood Serum and Urine of Patients with Renal Cell Carcinoma |
| Journal | Anticancer Research. 2016. |
| Authors | Iliev R, Fedorko M, Machackova T, Mlcochova H, Svoboda M,, Pacik D, Dolezel J, Stanik M, Slaby O, |
| Title | Comparison of MicroRNA Content in Plasma and Urine Indicates the Existence of a Transrenal Passage of Selected MicroRNAs |
| Journal | Advances in Experimental Medicine and Biology. 2016. |
| Authors | Pazourkova E, Pospisilova S, Svobodova I, Horinek A, Brisuda A, Soukup V, Hrbacek J, Capoun O, Mares J, Hanus T, Babjuk M, Korabecna M. |
| Title | MicroRNAs in urine supernatant as potential non-invasive markers for bladder cancer detection |
| Journal | Neoplasma. 2016. |
| Authors | Pospisilova S, Pazourkova E, Horinek A, Brisuda A, Svobodova I, Soukup V, Hrbacek J, Capoun O, Hanus T, Mares J, Korabecna M, Babjuk M. |
| Title | Biomarker Potential of Urine miR-451 at Different Stages of Diabetic Nephropathy |
| Journal | Journal of Diabetes & Metabolism. 2016. |
| Authors | Emel Isiktas Sayilar, Mustafa Gullulu, Ercan Tuncel, Hande Peynirci, Adem Alemdar, Berrin Tunca, Unal Egeli, Gulsah Cecener, Murat Bayindir and Gokhan Cosgun |
| Title | Live Cell MicroRNA Imaging Using Exonuclease III-Aided Recycling Amplification Based on Aggregation-Induced Emission Luminogens |
| Journal | ACS Appl. Mater. Interfaces. 2016. |
| Authors | Min, X., Zhang, M., Huang, F., Lou, X., & Xia, F |
| Title | Noninvasive urinary miRNA biomarkers for early detection of pancreatic adenocarcinoma |
| Journal | American Journal of Cancer Research. 2016. |
| Authors | Debernardi S, Massat NJ, Radon T1, Sangaralingam A, Banissi A, Ennis DP, Dowe T, Chelala C, Pereira SP, Kocher HM, Young BD, Bond-Smith G, Hutchins R, Crnogorac-Jurcevic T |
| Title | MicroRNA molecular profiling from matched tumor and bio-fluids in bladder cancer |
| Journal | Molecular Cancer. 2015. |
| Authors | David A. Armstrong, Benjamin B. Green, John D. Seigne, Alan R. Schned and Carmen J. Marsit |
| Title | MicroRNAs in urine are not biomarkers of multiple myeloma |
| Journal | Journal of Negative Results in Biomedicine. 2015. |
| Authors | Lenka Sedlaříková, Lenka Bešše, Soňa Novosadová, Veronika Kubaczková, Lenka Radová, Michal Staník, Marta Krejčí, Roman Hájek1 and Sabina Ševčíková |
| Title | Feasibility of urinary microRNA detection in breast cancer patients and its potential as an innovative non-invasive biomarker. |
| Journal | BMC Cancer. 2015. |
| Authors | Thalia Erbes, Marc Hirschfeld, Gerta Rücker, Markus Jaeger, Jasmin Boas, Severine Iborra, Sebastian Mayer, Gerald Gitsch and Elmar Stickeler. |
| Title | MicroRNA-21-5p upregulation in urine samples serves as novel biomarkers for early stage renal cell carcinoma patients diagnosis |
| Journal | Cancer Res. 2014. |
| Authors | Wei Meng, Hansjuerg Alder, Kirsteen Maclean, Simon Kirste, Petra Stegmaier, Anca Grosu, Arnab Chakravarti, Tim Lautenschlaeger |
| Title | Urinary microRNA-29 correlates with urinary albumin in type 2 diabetes mellitus. |
| Journal | Chinese Journal of Nephrology. 2014. |
| Authors | Meirong Z, Hui P, Wenbo Z, Cheng W, Jun Z, Xun L, Yuanqing L, Paudel S, Qianqian W, Tanqi L. |
| Title | Urinary miR-29 Correlates with Albuminuria and Carotid Intima-Media Thickness in Type 2 Diabetes Patients. |
| Journal | PLoS One. 2013. |
| Authors | Peng H, Zhong M, Zhao W, Wang C, Zhang J, Liu X, Li Y, Paudel S, Wang Q, Lou T. |
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