ProteoSpin™ Urine Protein Concentration Kits

Cat. 17400, 52300, 21600
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ProteoSpin™ Urine Protein Concentration Kits

Cat. 17400, 52300, 21600

For the rapid and efficient concentration of total proteins from urine samples

  • Simultaneous concentration, desalting and buffer exchange of total urinary proteins
  • No molecular weight cutoff allows for isolation of all sizes of proteins and peptides
  • Purification is based on spin column chromatography that uses Norgen’s resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

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ProteoSpin™ Urine Protein Concentration Micro Kit
(Cat. 17400)
25 preps
ProteoSpin™ Urine Protein Concentration Midi Kit
(Cat. 52300)
10 preps
ProteoSpin™ Urine Protein Concentration Maxi Kit
(Cat. 21600)
4 preps

Kit Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Kit Size

  • Simultaneous concentration, desalting and buffer exchange of total urinary proteins
  • No molecular weight cutoff allows for isolation of all sizes of proteins and peptides
  • Purification is based on spin column chromatography that uses Norgen’s resin separation matrix

ProteoSpin™ Urine Protein Concentration Kits

These kits concentrate urine proteins while simultaneously removing salts, urea, and other urine contaminants. There is no molecular weight cut-off and therefore the columns capture total urinary proteins and peptides of all sizes making them ideal for biomarker discovery work, differential expression of proteins in various diseases, or other diagnostic research. The columns are convenient, rapid and easy to use and thus offer significant time savings over classic dialysis protocols. The resulting high-quality protein sample is concentrated and free from the original sample salts, thus preparing the sample conveniently for downstream proteomic applications including SDS-PAGE, 2D Gels, MALDI-TOF, LC/MS, LC/MS/MS, whole protein mass spectrometry, western blotting, protein microarrays, and more.

ProteoSpin™ Urine Protein Concentration Micro Kit

Each spin column is able to concentrate and desalt up to 200 μg of urine proteins. Twelve samples can be processed in 20 minutes.

ProteoSpin™ Urine Protein Concentration Midi Kit

The ProteoSpin™ Urine Protein Concentration Midi Kit provides a fast and simple procedure for concentrating dilute solutions of urine proteins from 1 to 5 mL inputs of urine. Each mini spin column is able to concentrate and desalt up to 3 mg of urine proteins in 30 minutes.

ProteoSpin™ Urine Protein Concentration Maxi Kit

The ProteoSpin™ Urine Protein Concentration Maxi Kit provides a fast and simple procedure for concentrating dilute solutions of urine proteins from 2 to 20 mL inputs of urine. Each maxi spin column is able to concentrate and desalt up to 4 mg of urine proteins in 45 minutes.

Kit Specifications

You have selected: Cat. 17400
Kit Specifications
Maximum Urine Input Volume
1 mL
Maximum Recovered Protein
200 μg
Elution Volume
100 μL
Time to Process 12 Samples
20 minutes


Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. This kit is stable for 2 years after the date of shipment.

You have selected: Cat. 52300
Kit Specifications
Minimum Urine Volume Input
1 mL
Maximum Urine Volume Input
5 mL
Maximum Recovered Proteins (5 mL Urine)
3 mg*
Elution Volume
200 - 1000 μL
Time to Complete 10 Purifications
30 minutes

 *Yield will depend on the health status of the donor.

 
Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solution should be stored at 4°C. This kit is stable for 2 years after the date of shipment.
You have selected: Cat. 21600
Kit Specifications
Maximum Urine Input Volume
20 mL
Maximum Recovered Protein
4 mg
Minimum Elution Volume
2 mL
Time to Process 4 Samples
45 minutes

Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solution should be stored at 4°C. This kit is stable for 2 years after the date of shipment.

Supporting Data

Click for expanded view

Component Cat. 17400 (25 preps) Cat. 52300 (10 preps) Cat. 21600 (4 preps)
Wash Solution C 60 mL 60 mL 130 mL
Binding Buffer A 4 mL 4 mL 8 mL
Elution Buffer C 8 mL 30 mL 30 mL
Protein Neutralizer 4 mL 4 mL 4 mL
Micro Spin Columns 25 - -
Midi Spin Columns (assembled with collection tubes) - 10 -
Maxi Spin Columns (assembled with collection tubes) - - 4
Collection Tubes 25 - -
Elution Tubes (1.7 mL) 25 - -
Midi Elution Tubes - 10 -
Elution Tubes (50 mL) - - 4
Product Insert 1 1 1

Micro (17400)

Title Peptidomics analysis reveals changes in small urinary peptides in patients with interstitial cystitis/bladder pain syndrome
Journal Scientific Reports. 2022.
Authors Md Shadman Ridwan Abid, Haowen Qiu, Bridget A. Tripp, Aline de Lima Leite, Heidi E. Roth, Jiri Adamec, Robert Powers & James W. Checco
Title KeepEX, a simple dilution protocol for improving extracellular vesicle yields from urine
Journal European Journal of Pharmaceutical Sciences. 2016.
Authors Puhka M, Nordberg ME, Valkonen S, Rannikko A, Kallioniemi O, Siljander P, Hällström TA
Title Mass Spectrometric Quantification of Urinary Netrin-1 Protein in Renal Transplant Recipients
Journal Current Proteomics. 2016.
Authors Ozcan, Filiz; Akbas, Halide; Suleymanlar, Gultekin; Aslan, Mutay; Yucel, Gultekin
Title Cisplatin nephrotoxicity in male beagle dogs: next-generation protein kidney safety biomarker tissue expression and related changes in urine
Journal Toxicology Research. 2016.
Authors J. E. McDuffie, Y. Chen, J. Y. Ma, S. Lee, K. M. Lynch, D. M. Hamlin, L. Nguyen, M. Rizzolio, M. Sonee and S. Snook
Title Mass spectrometric quantification of urinary human liver fatty acid binding protein in renal transplant recipients
Journal Rapid Communications in Mass Spectrometry. 2016.
Authors Filiz Ozcan, Halide Akbas, Ebru Kırac, Gultekin Suleymanlar, Mutay Aslan, andGultekin Yucel
Title Comparison of Depletion Strategies for the Enrichment of Low-Abundance Proteins in Urine
Journal PLOS ONE. 2015.
Authors Szymon Filip, Konstantinos Vougas, Jerome Zoidakis, Agnieszka Latosinska, William Mullen, Goce Spasovski, Harald Mischak, Antonia Vlahou, Joachim Jankowski
Title Potential Urinary Protein Biomarker Candidates for the Accurate Detection of Prostate Cancer among Benign Prostatic Hyperplasia Patients
Journal Journal of Cancer. 2014.
Authors Taha A Haj-Ahmad, Moemen AK Abdalla, Yousef Haj-Ahmad
Title Promising Candidate Urinary MicroRNA Biomarkers for the Early Detection of Hepatocellular Carcinoma among High-Risk Hepatitis C Virus Egyptian Patients.
Journal Journal of Cancer. 2012.
Authors Abdalla M, Haj-Ahmad Y.
Title Human Alpha Defensin 5 Expression in the Human Kidney and Urinary Tract.
Journal PLOS One. 2012.
Authors Spencer J, Hains D, Porter E, Bevins C, DiRosario J, Becknell B, Wang H, Schwaderer A.
Title Ribonuclease 7 is a potent antimicrobial peptide within the human urinary tract.
Journal Kidney International. 2011.
Authors Spencer JD, Schwaderer AL, Dirosario JD, McHugh KM, McGillivary G, Justice SS, Carpenter AR, Baker PB, Harder J, Hains DS.
Title Plasmin activates epithelial Na+ channels by cleaving the gamma subunit.
Journal Journal of Biological Chemistry. 2008.
Authors Lu CY, Chang YM, Tseng WL, Feng CH, Lu CY.
Title Large-scale protein identification of human urine proteome by multi-dimensional LC and MS/MS.
Journal Proteomics Clinical Applications. 2007.
Authors Chen YT, Tsao CY, Li JM, Tsai CY, Chiu SF, Tseng TL.

Maxi (21600)

Title Treatment of Collagen Defects Using Protein Solutions
Journal United States Patent Application 20160166645. 2016.
Authors Matuska A, O'shaughnessey K, Woodell-may JE
Title Methods of Treating Pain Using Protein Solutions
Journal United States Patent Application 20160136245. 2016.
Authors Toler K, King W, Woodell-may JE
Title Profiling of urinary proteins in Karan Fries cows reveals more than 1550 proteins.
Journal Journal of Proteomics. 2015.
Authors Bathla S, Rawat P, Baithalu R, Yadav ML, Naru J, Tiwari A, Kumar S, Balhara AK, Singh S, Chaudhary S, Kumar R, Lotfan M, Behare P, Phulia SK, Mohanty TK, Kaushik JK, Nallapeta S, Singh I, Ambatipudi SK, Mohanty AK
Title Plasmin activates epithelial Na+ channels by cleaving the gamma subunit.
Journal Journal of Biological Chemistry. 2008.
Authors Lu CY, Chang YM, Tseng WL, Feng CH, Lu CY.
Title Large-scale protein identification of human urine proteome by multi-dimensional LC and MS/MS.
Journal Proteomics Clinical Applications. 2007.
Authors Chen YT, Tsao CY, Li JM, Tsai CY, Chiu SF, Tseng TL.

ProteoSpin™ Urine Protein Concentration Kits

These kits concentrate urine proteins while simultaneously removing salts, urea, and other urine contaminants. There is no molecular weight cut-off and therefore the columns capture total urinary proteins and peptides of all sizes making them ideal for biomarker discovery work, differential expression of proteins in various diseases, or other diagnostic research. The columns are convenient, rapid and easy to use and thus offer significant time savings over classic dialysis protocols. The resulting high-quality protein sample is concentrated and free from the original sample salts, thus preparing the sample conveniently for downstream proteomic applications including SDS-PAGE, 2D Gels, MALDI-TOF, LC/MS, LC/MS/MS, whole protein mass spectrometry, western blotting, protein microarrays, and more.

ProteoSpin™ Urine Protein Concentration Micro Kit

Each spin column is able to concentrate and desalt up to 200 μg of urine proteins. Twelve samples can be processed in 20 minutes.

ProteoSpin™ Urine Protein Concentration Midi Kit

The ProteoSpin™ Urine Protein Concentration Midi Kit provides a fast and simple procedure for concentrating dilute solutions of urine proteins from 1 to 5 mL inputs of urine. Each mini spin column is able to concentrate and desalt up to 3 mg of urine proteins in 30 minutes.

ProteoSpin™ Urine Protein Concentration Maxi Kit

The ProteoSpin™ Urine Protein Concentration Maxi Kit provides a fast and simple procedure for concentrating dilute solutions of urine proteins from 2 to 20 mL inputs of urine. Each maxi spin column is able to concentrate and desalt up to 4 mg of urine proteins in 45 minutes.

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 17400
Kit Specifications
Maximum Urine Input Volume
1 mL
Maximum Recovered Protein
200 μg
Elution Volume
100 μL
Time to Process 12 Samples
20 minutes


Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. This kit is stable for 2 years after the date of shipment.

You have selected: Cat. 52300
Kit Specifications
Minimum Urine Volume Input
1 mL
Maximum Urine Volume Input
5 mL
Maximum Recovered Proteins (5 mL Urine)
3 mg*
Elution Volume
200 - 1000 μL
Time to Complete 10 Purifications
30 minutes

 *Yield will depend on the health status of the donor.

 
Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solution should be stored at 4°C. This kit is stable for 2 years after the date of shipment.
You have selected: Cat. 21600
Kit Specifications
Maximum Urine Input Volume
20 mL
Maximum Recovered Protein
4 mg
Minimum Elution Volume
2 mL
Time to Process 4 Samples
45 minutes

Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solution should be stored at 4°C. This kit is stable for 2 years after the date of shipment.

Component Cat. 17400 (25 preps) Cat. 52300 (10 preps) Cat. 21600 (4 preps)
Wash Solution C 60 mL 60 mL 130 mL
Binding Buffer A 4 mL 4 mL 8 mL
Elution Buffer C 8 mL 30 mL 30 mL
Protein Neutralizer 4 mL 4 mL 4 mL
Micro Spin Columns 25 - -
Midi Spin Columns (assembled with collection tubes) - 10 -
Maxi Spin Columns (assembled with collection tubes) - - 4
Collection Tubes 25 - -
Elution Tubes (1.7 mL) 25 - -
Midi Elution Tubes - 10 -
Elution Tubes (50 mL) - - 4
Product Insert 1 1 1

Micro (17400)

Title Peptidomics analysis reveals changes in small urinary peptides in patients with interstitial cystitis/bladder pain syndrome
Journal Scientific Reports. 2022.
Authors Md Shadman Ridwan Abid, Haowen Qiu, Bridget A. Tripp, Aline de Lima Leite, Heidi E. Roth, Jiri Adamec, Robert Powers & James W. Checco
Title KeepEX, a simple dilution protocol for improving extracellular vesicle yields from urine
Journal European Journal of Pharmaceutical Sciences. 2016.
Authors Puhka M, Nordberg ME, Valkonen S, Rannikko A, Kallioniemi O, Siljander P, Hällström TA
Title Mass Spectrometric Quantification of Urinary Netrin-1 Protein in Renal Transplant Recipients
Journal Current Proteomics. 2016.
Authors Ozcan, Filiz; Akbas, Halide; Suleymanlar, Gultekin; Aslan, Mutay; Yucel, Gultekin
Title Cisplatin nephrotoxicity in male beagle dogs: next-generation protein kidney safety biomarker tissue expression and related changes in urine
Journal Toxicology Research. 2016.
Authors J. E. McDuffie, Y. Chen, J. Y. Ma, S. Lee, K. M. Lynch, D. M. Hamlin, L. Nguyen, M. Rizzolio, M. Sonee and S. Snook
Title Mass spectrometric quantification of urinary human liver fatty acid binding protein in renal transplant recipients
Journal Rapid Communications in Mass Spectrometry. 2016.
Authors Filiz Ozcan, Halide Akbas, Ebru Kırac, Gultekin Suleymanlar, Mutay Aslan, andGultekin Yucel
Title Comparison of Depletion Strategies for the Enrichment of Low-Abundance Proteins in Urine
Journal PLOS ONE. 2015.
Authors Szymon Filip, Konstantinos Vougas, Jerome Zoidakis, Agnieszka Latosinska, William Mullen, Goce Spasovski, Harald Mischak, Antonia Vlahou, Joachim Jankowski
Title Potential Urinary Protein Biomarker Candidates for the Accurate Detection of Prostate Cancer among Benign Prostatic Hyperplasia Patients
Journal Journal of Cancer. 2014.
Authors Taha A Haj-Ahmad, Moemen AK Abdalla, Yousef Haj-Ahmad
Title Promising Candidate Urinary MicroRNA Biomarkers for the Early Detection of Hepatocellular Carcinoma among High-Risk Hepatitis C Virus Egyptian Patients.
Journal Journal of Cancer. 2012.
Authors Abdalla M, Haj-Ahmad Y.
Title Human Alpha Defensin 5 Expression in the Human Kidney and Urinary Tract.
Journal PLOS One. 2012.
Authors Spencer J, Hains D, Porter E, Bevins C, DiRosario J, Becknell B, Wang H, Schwaderer A.
Title Ribonuclease 7 is a potent antimicrobial peptide within the human urinary tract.
Journal Kidney International. 2011.
Authors Spencer JD, Schwaderer AL, Dirosario JD, McHugh KM, McGillivary G, Justice SS, Carpenter AR, Baker PB, Harder J, Hains DS.
Title Plasmin activates epithelial Na+ channels by cleaving the gamma subunit.
Journal Journal of Biological Chemistry. 2008.
Authors Lu CY, Chang YM, Tseng WL, Feng CH, Lu CY.
Title Large-scale protein identification of human urine proteome by multi-dimensional LC and MS/MS.
Journal Proteomics Clinical Applications. 2007.
Authors Chen YT, Tsao CY, Li JM, Tsai CY, Chiu SF, Tseng TL.

Maxi (21600)

Title Treatment of Collagen Defects Using Protein Solutions
Journal United States Patent Application 20160166645. 2016.
Authors Matuska A, O'shaughnessey K, Woodell-may JE
Title Methods of Treating Pain Using Protein Solutions
Journal United States Patent Application 20160136245. 2016.
Authors Toler K, King W, Woodell-may JE
Title Profiling of urinary proteins in Karan Fries cows reveals more than 1550 proteins.
Journal Journal of Proteomics. 2015.
Authors Bathla S, Rawat P, Baithalu R, Yadav ML, Naru J, Tiwari A, Kumar S, Balhara AK, Singh S, Chaudhary S, Kumar R, Lotfan M, Behare P, Phulia SK, Mohanty TK, Kaushik JK, Nallapeta S, Singh I, Ambatipudi SK, Mohanty AK
Title Plasmin activates epithelial Na+ channels by cleaving the gamma subunit.
Journal Journal of Biological Chemistry. 2008.
Authors Lu CY, Chang YM, Tseng WL, Feng CH, Lu CY.
Title Large-scale protein identification of human urine proteome by multi-dimensional LC and MS/MS.
Journal Proteomics Clinical Applications. 2007.
Authors Chen YT, Tsao CY, Li JM, Tsai CY, Chiu SF, Tseng TL.

 


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