For convenient purification of RNA and DNA from microorganisms in water samples

  • This kit includes filters. For the isolation kit that does not include filters, see Cat. 26480.
  • Isolate total DNA and RNA from all microorganisms found in water, including bacteria, fungi and algae
  • RNA and DNA are both column purified simultaneously using the same column
  • Elution contains concentrated DNA and RNA without the need for further precipitation
  • Complete RNA (including microRNA) without phenol
  • Isolated RNA and DNA are of high quality and integrity for all downstream applications
  • Available in 0.45 µm and 0.22 µm filter formats

ItemsCat. #Size
Water RNA/DNA Purification Kit - 0.45 µm 26450 25 preps
Water RNA/DNA Purification Kit - 0.22 µm 26400 25 preps

Water RNA/DNA Purification Kit - 0.45 µm and 0.22 µm

These kits provide a convenient and rapid method for the isolation of microorganisms from water samples. The kits allows for the rapid isolation and purification of total RNA and DNA simultaneously from the microorganisms found in different types of water samples. The total RNA and DNA (including genomic DNA) are isolated from all the microorganisms found in the water, including bacteria, fungi and algae without the use of any inhibitory organic substances. The kit purifies genomic DNA, and all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The purified RNA and DNA are highly concentrated, and can be used directly in a number of downstream applications including PCR, qPCR, RT-PCR, qRT-PCR, Northern blotting, Southern blotting and sequencing reactions.

Kit Specifications
Minimum Water Input
10 mL
Maximum Water Input
100 mL
Maximum Filter Column Loading Volume
20 mL
Maximum Spin Column Loading Volume
650 µL
Elution Volume
100 µL
Time to Complete 10 Purifications
45 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.


Kit Components

Component

Cat. 26400 (25 preps)
Lysis Solution 15 mL
Nucleic Acid Wash Solution 11 mL
Enzyme Incubation Buffer 4 mL
Nucleic Acid Elution Buffer 4 mL
Mini Spin Columns 25
Filter Columns 25
Bead Tubes 25
Collection Tubes 25
Elution Tubes (1.7 mL) 25
Product Insert 1

Component

Cat. 26450 (25 preps)
Lysis Buffer E 15 mL
Wash Solution A 18 mL
Enzyme Incubation Buffer B 6 mL
Elution Buffer H 6 mL
Mini Spin Columns 25
Filter Columns 25
Bead Tubes 25
Collection Tubes 25
Elution Tubes (1.7 mL) 25
Product Insert 1

Title The Source of the River as a Nursery for Microbial Diversity
Journal PLOS ONE. 2015.
Authors Luiz Felipe Valter de Oliveira, Rogério Margis
Title Assessment of the biological invasion risks associated with a massive outdoor cultivation of the green alga, Pseudochoricystis ellipsoidea
Journal Algal Research. 2015.
Authors Izumi Matsuwaki, Shigeaki Harayama, Misako Kato
Title Characteristics of biofilm development in an operating rainwater storage tank.
Journal Environmental Earth Sciences. 2014.
Authors Kim M, Han M.
Title Detection of diverse aquatic microbes in blood and organs of drowning victims: first metagenomic approach using high-throughput 454-pyrosequencing.
Journal Forensic Science International. 2012.
Authors Kakizaki E, Ogura Y, Kozawa S, Nishida S, Uchiyama T, Hayashi T, Yukawa N.