Water RNA/DNA Purification Kit - 0.45 µm and 0.22 µm (Cat. 26450, 26400)

Products
Multiple-Analyte Kits
 

Purchasing Information

  • This kit includes filters. For the isolation kit that does not include filters, see Cat. 26480.
  • Isolate total DNA and RNA from all microorganisms found in water, including bacteria, fungi and algae
  • RNA and DNA are both column purified simultaneously using the same column 
  • Elution contains concentrated DNA and RNA without the need for further precipitation 
  • Complete RNA (including microRNA) without phenol 
  • Isolated RNA and DNA are of high quality and integrity for all downstream applications
  • Available in 0.45 µm and 0.22 µm filter formats
  • Purification is based on spin column chromatography that uses Norgen’s proprietary Silicon Carbide resin separation matrix
 

For research use only and NOT intended for in vitro diagnostics.

For convenient purification of RNA and DNA from microorganisms in water samples

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Water RNA/DNA Purification Kit - 0.45 µm (Cat. 26450) 25 preps
Water RNA/DNA Purification Kit - 0.22 µm (Cat. 26400) 25 preps

  • This kit includes filters. For the isolation kit that does not include filters, see Cat. 26480.
  • Isolate total DNA and RNA from all microorganisms found in water, including bacteria, fungi and algae
  • RNA and DNA are both column purified simultaneously using the same column 
  • Elution contains concentrated DNA and RNA without the need for further precipitation 
  • Complete RNA (including microRNA) without phenol 
  • Isolated RNA and DNA are of high quality and integrity for all downstream applications
  • Available in 0.45 µm and 0.22 µm filter formats
  • Purification is based on spin column chromatography that uses Norgen’s proprietary Silicon Carbide resin separation matrix
 

Click for expanded view


Water RNA/DNA Purification Kit - 0.45 µm and 0.22 µm

These kits provide a convenient and rapid method for the isolation of microorganisms from water samples. The kits allows for the rapid isolation and purification of total RNA and DNA simultaneously from the microorganisms found in different types of water samples. The total RNA and DNA (including genomic DNA) are isolated from all the microorganisms found in the water, including bacteria, fungi and algae without the use of any inhibitory organic substances.  The kit purifies genomic DNA, and all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA).  The purified RNA and DNA are highly concentrated, and can be used directly in a number of downstream applications including PCR, qPCR, RT-PCR, qRT-PCR, Northern blotting, Southern blotting and sequencing reactions.

Kit Specifications
Minimum Water Input
10 mL
Maximum Water Input
100 mL
Maximum Filter Column Loading Volume
20 mL
Maximum Spin Column Loading Volume
650 µL
Elution Volume
100 µL
Time to Complete 10 Purifications
45 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature.  These reagents should remain stable for at least 1 year in their unopened containers.
 

 


Title The Source of the River as a Nursery for Microbial Diversity
Journal PLOS ONE. 2015.
Authors Luiz Felipe Valter de Oliveira, Rogério Margis
Title Assessment of the biological invasion risks associated with a massive outdoor cultivation of the green alga, Pseudochoricystis ellipsoidea
Journal Algal Research. 2015.
Authors Izumi Matsuwaki, Shigeaki Harayama, Misako Kato
Title Characteristics of biofilm development in an operating rainwater storage tank.
Journal Environmental Earth Sciences. 2014.
Authors Kim M, Han M.
Title Detection of diverse aquatic microbes in blood and organs of drowning victims: first metagenomic approach using high-throughput 454-pyrosequencing.
Journal Forensic Science International. 2012.
Authors Kakizaki E, Ogura Y, Kozawa S, Nishida S, Uchiyama T, Hayashi T, Yukawa N.

Want a discount?

Create your account to receive 15% off on your first purchase!

Full Kits

Water RNA/DNA Purification Kit - 0.45 µm (Cat. 26450) 25 preps
Water RNA/DNA Purification Kit - 0.22 µm (Cat. 26400) 25 preps

  • This kit includes filters. For the isolation kit that does not include filters, see Cat. 26480.
  • Isolate total DNA and RNA from all microorganisms found in water, including bacteria, fungi and algae
  • RNA and DNA are both column purified simultaneously using the same column 
  • Elution contains concentrated DNA and RNA without the need for further precipitation 
  • Complete RNA (including microRNA) without phenol 
  • Isolated RNA and DNA are of high quality and integrity for all downstream applications
  • Available in 0.45 µm and 0.22 µm filter formats
  • Purification is based on spin column chromatography that uses Norgen’s proprietary Silicon Carbide resin separation matrix
 

Click for expanded view


Water RNA/DNA Purification Kit - 0.45 µm and 0.22 µm

These kits provide a convenient and rapid method for the isolation of microorganisms from water samples. The kits allows for the rapid isolation and purification of total RNA and DNA simultaneously from the microorganisms found in different types of water samples. The total RNA and DNA (including genomic DNA) are isolated from all the microorganisms found in the water, including bacteria, fungi and algae without the use of any inhibitory organic substances.  The kit purifies genomic DNA, and all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA).  The purified RNA and DNA are highly concentrated, and can be used directly in a number of downstream applications including PCR, qPCR, RT-PCR, qRT-PCR, Northern blotting, Southern blotting and sequencing reactions.

Kit Specifications
Minimum Water Input
10 mL
Maximum Water Input
100 mL
Maximum Filter Column Loading Volume
20 mL
Maximum Spin Column Loading Volume
650 µL
Elution Volume
100 µL
Time to Complete 10 Purifications
45 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature.  These reagents should remain stable for at least 1 year in their unopened containers.
 

 

Title The Source of the River as a Nursery for Microbial Diversity
Journal PLOS ONE. 2015.
Authors Luiz Felipe Valter de Oliveira, Rogério Margis
Title Assessment of the biological invasion risks associated with a massive outdoor cultivation of the green alga, Pseudochoricystis ellipsoidea
Journal Algal Research. 2015.
Authors Izumi Matsuwaki, Shigeaki Harayama, Misako Kato
Title Characteristics of biofilm development in an operating rainwater storage tank.
Journal Environmental Earth Sciences. 2014.
Authors Kim M, Han M.
Title Detection of diverse aquatic microbes in blood and organs of drowning victims: first metagenomic approach using high-throughput 454-pyrosequencing.
Journal Forensic Science International. 2012.
Authors Kakizaki E, Ogura Y, Kozawa S, Nishida S, Uchiyama T, Hayashi T, Yukawa N.

 

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