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For research use only and NOT intended for in vitro diagnostics.
For convenient purification of RNA and DNA from microorganisms in water samples
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Water RNA/DNA Purification Kit - 0.45 µm and 0.22 µm
These kits provide a convenient and rapid method for the isolation of microorganisms from water samples. The kits allows for the rapid isolation and purification of total RNA and DNA simultaneously from the microorganisms found in different types of water samples. The total RNA and DNA (including genomic DNA) are isolated from all the microorganisms found in the water, including bacteria, fungi and algae without the use of any inhibitory organic substances. The kit purifies genomic DNA, and all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The purified RNA and DNA are highly concentrated, and can be used directly in a number of downstream applications including PCR, qPCR, RT-PCR, qRT-PCR, Northern blotting, Southern blotting and sequencing reactions.
Kit Specifications
|
|
Minimum Water Input |
10 mL
|
Maximum Water Input |
100 mL
|
Maximum Filter Column Loading Volume |
20 mL
|
Maximum Spin Column Loading Volume |
650 µL
|
Elution Volume |
100 µL
|
Time to Complete 10 Purifications |
45 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
Title | The Source of the River as a Nursery for Microbial Diversity |
Journal | PLOS ONE. 2015. |
Authors | Luiz Felipe Valter de Oliveira, Rogério Margis |
Title | Assessment of the biological invasion risks associated with a massive outdoor cultivation of the green alga, Pseudochoricystis ellipsoidea |
Journal | Algal Research. 2015. |
Authors | Izumi Matsuwaki, Shigeaki Harayama, Misako Kato |
Title | Characteristics of biofilm development in an operating rainwater storage tank. |
Journal | Environmental Earth Sciences. 2014. |
Authors | Kim M, Han M. |
Title | Amplicon pyrosequencing and ion torrent sequencing of wild duck eubacterial microbiome from fecal samples reveals numerous species linked to human and animal diseases. |
Journal | F1000 Research. 2013. |
Authors | Strong T, Dowd S, Gutierrez A, Molnar D, Coffman J. |
Title | Detection of diverse aquatic microbes in blood and organs of drowning victims: first metagenomic approach using high-throughput 454-pyrosequencing. |
Journal | Forensic Science International. 2012. |
Authors | Kakizaki E, Ogura Y, Kozawa S, Nishida S, Uchiyama T, Hayashi T, Yukawa N. |
Want a discount?
Create your account to receive 15% off on your first purchase!
Water RNA/DNA Purification Kit - 0.45 µm and 0.22 µm
These kits provide a convenient and rapid method for the isolation of microorganisms from water samples. The kits allows for the rapid isolation and purification of total RNA and DNA simultaneously from the microorganisms found in different types of water samples. The total RNA and DNA (including genomic DNA) are isolated from all the microorganisms found in the water, including bacteria, fungi and algae without the use of any inhibitory organic substances. The kit purifies genomic DNA, and all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The purified RNA and DNA are highly concentrated, and can be used directly in a number of downstream applications including PCR, qPCR, RT-PCR, qRT-PCR, Northern blotting, Southern blotting and sequencing reactions.
Kit Specifications
|
|
Minimum Water Input |
10 mL
|
Maximum Water Input |
100 mL
|
Maximum Filter Column Loading Volume |
20 mL
|
Maximum Spin Column Loading Volume |
650 µL
|
Elution Volume |
100 µL
|
Time to Complete 10 Purifications |
45 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
Title | The Source of the River as a Nursery for Microbial Diversity |
Journal | PLOS ONE. 2015. |
Authors | Luiz Felipe Valter de Oliveira, Rogério Margis |
Title | Assessment of the biological invasion risks associated with a massive outdoor cultivation of the green alga, Pseudochoricystis ellipsoidea |
Journal | Algal Research. 2015. |
Authors | Izumi Matsuwaki, Shigeaki Harayama, Misako Kato |
Title | Characteristics of biofilm development in an operating rainwater storage tank. |
Journal | Environmental Earth Sciences. 2014. |
Authors | Kim M, Han M. |
Title | Amplicon pyrosequencing and ion torrent sequencing of wild duck eubacterial microbiome from fecal samples reveals numerous species linked to human and animal diseases. |
Journal | F1000 Research. 2013. |
Authors | Strong T, Dowd S, Gutierrez A, Molnar D, Coffman J. |
Title | Detection of diverse aquatic microbes in blood and organs of drowning victims: first metagenomic approach using high-throughput 454-pyrosequencing. |
Journal | Forensic Science International. 2012. |
Authors | Kakizaki E, Ogura Y, Kozawa S, Nishida S, Uchiyama T, Hayashi T, Yukawa N. |