Water RNA/DNA Purification Kits For convenient purification of RNA and DNA from microorganisms in water samples For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Water RNA/DNA Purification Kits For convenient purification of RNA and DNA from microorganisms in water samples SKU 26400 Format Spin Column + 0.22 µm Filter Spin Column + 0.45 µm Filter Spin Column Size 25 Preps Contact Distributor Overview Details Documentation Citations Overview Isolate total DNA and RNA from all microorganisms found in water, including bacteria, fungi and algae RNA and DNA are both column purified simultaneously using the same column Elution contains concentrated DNA and RNA without the need for further precipitation Complete RNA (including microRNA) without phenol Isolated RNA and DNA are of high quality and integrity for all downstream applications Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix These kits provide a convenient and rapid method for the isolation of microorganisms from water samples. The kits allows for the rapid isolation and purification of total RNA and DNA simultaneously from the microorganisms found in different types of water samples. The total RNA and DNA (including genomic DNA) are isolated from all the microorganisms found in the water, including bacteria, fungi and algae without the use of any inhibitory organic substances The kit purifies genomic DNA, and all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The purified RNA and DNA are highly concentrated, and can be used directly in a number of downstream applications including PCR, qPCR, RT-PCR, qRT-PCR, Northern blotting, Southern blotting and sequencing reactions. Water RNA/DNA Purification Kit (Spin Column + Filters Kits) These kits are available with 0.45 μm or 0.22 μm filter formats for capturing microorganisms present in the water. Water RNA/DNA Purification Kit (Spin Column) This kit is sold without filters columns. Details Supporting Data Previous Figure 1. High Yield and Purity of RNA and DNA. Total RNA and DNA were simultaneously isolated from 50 mL of water sample containing 107 cfu/mL E.coli using Norgen's Water RNA/DNA Purification Kit and subsequently run on gels for visual analysis. Panel A shows 10 µL aliquots (no RNase treatment) of the 50 µL elutions run on a 1% TAE agarose gel. Genomic DNA and 16S and 23S rRNA bands were visible. Panel B shows 5 µL aliquots (on-column DNase was applied) of the elution run on a 1.5% formaldehyde agarose gel. 16S and 23S rRNA was seen without DNA contamination. From observing the gels it can be seen that the kit allows for the isolation and purification of high yields of concentrated and high quality RNA and DNA. Figure 2. Detection of E. coli 16S rDNA by Real-Time PCR (SYBR Green) Fifty mL water samples spiked with increasing amounts of E.coli were filtrated through the provided 0.45 µm water filter columns, and RNase-treated DNA was isolated from the filter by following Norgen's Water RNA/DNA Purification Kit manual. Next, 2 µL of DNA from the 50 µL elutions was mixed in 18 µL of total real-time PCR reaction master mix and real-time PCR was performed (95°C for 3 minutes and 40 cycles at 95°C for 15 second and 60°C for 30 seconds.). All tested water samples containing different E.coli amounts were found to correspond to the real-time PCR results. E.coli was specifically detected by E. coli 16S rDNA primers down to 102 cfu/ml, indicating the quality of DNA and the efficiency of filtering system. Next Click for expanded view Kit Specifications Minimum Water Input 10 mL Maximum Water Input 100 mL Maximum Filter Column Loading Volume 20 mL Maximum Spin Column Loading Volume 650 µL Elution Volume 100 µL Time to Complete 10 Purifications 45 minutes Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment. Component Cat. 26400 (25 preps) Cat. 26450 (25 preps) Cat. 26480 (50 preps) Lysis Buffer E 15 mL 15 mL 2 x 15 mL Wash Solution A 18 mL 18 mL 38 mL Enzyme Incubation Buffer B 6 mL 6 mL 6 mL Elution Buffer H 6 mL 6 mL 6 mL Mini Spin Columns 25 25 50 Filter Columns (0.22 μL) 25 - - Filter Columns (0.45 μL) - 25 - Bead Tubes 25 25 50 Collection Tubes 25 25 50 Elution Tubes (1.7 mL) 25 25 50 Product Insert 1 1 1 Documentation PROTOCOLS PI26400-12 Water RNA DNA Purification Kit.pdf PI26450-10 Water RNA DNA Purification Kit.pdf PI26480-3 Water RNA DNA Purification Kit.pdf SAFETY DATA SHEETS 26400_26450_SDS.pdf POSTERS water_rna_dna_purification_kit_045_m_a_novel_technology_to_extract_nucleic_acids_from_different_environmental_samples_including_plants_water_and_soil_posters_70.pdf FLYERS Water_RNA_DNA_Purification_Kit_1.0_Jul2018.pdf RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class Citations Powered by Bioz See more details on Bioz Related Products RNase-Free DNase I Kit Soil DNA Isolation Kit Soil DNA Isolation 96-Well Kit
Water RNA/DNA Purification Kits For convenient purification of RNA and DNA from microorganisms in water samples For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Water RNA/DNA Purification Kits For convenient purification of RNA and DNA from microorganisms in water samples SKU 26400 Format Spin Column + 0.22 µm Filter Spin Column + 0.45 µm Filter Spin Column Size 25 Preps Contact Distributor