Water RNA/DNA Purification Kits (Cat. 26400, 26450, 26480)
Water RNA/DNA Purification Kits
![Water RNA/DNA Purification Kit (with 0.45 μm fiters)](https://norgenbiotek.com/sites/default/files/styles/original/public/images/product_images/26450-Reflection-web.jpg?itok=BZxfMHN9" "Water RNA/DNA Purification Kit (with 0.45 μm fiters)")
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Water RNA/DNA Purification Kits
For convenient purification of RNA and DNA from microorganisms in water samples
- Isolate total DNA and RNA from all microorganisms found in water, including bacteria, fungi and algae
- RNA and DNA are both column purified simultaneously using the same column
- Elution contains concentrated DNA and RNA without the need for further precipitation
- Complete RNA (including microRNA) without phenol
- Isolated RNA and DNA are of high quality and integrity for all downstream applications
- Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
For research use only and NOT intended for in vitro diagnostics.
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(Cat. 26400) 25 preps
(Cat. 26450) 25 preps
(Cat. 26480) 50 preps
Product Format Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.
Product Size
Kit Specifications
|
Kit Specifications
|
|
| Minimum Water Input |
10 mL
|
| Maximum Water Input |
100 mL
|
| Maximum Filter Column Loading Volume |
20 mL
|
| Maximum Spin Column Loading Volume |
650 µL
|
| Elution Volume |
100 µL
|
| Time to Complete 10 Purifications |
45 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment.
|
Kit Specifications
|
|
| Minimum Water Input |
10 mL
|
| Maximum Water Input |
100 mL
|
| Maximum Filter Column Loading Volume |
20 mL
|
| Maximum Spin Column Loading Volume |
650 µL
|
| Elution Volume |
100 µL
|
| Time to Complete 10 Purifications |
45 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment.
|
Kit Specifications
|
|
| Maximum Spin Column Loading Volume |
650 µL
|
| Elution Volume |
100 µL
|
| Time to Complete 10 Purifications |
45 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Supporting Data
| Component | Cat. 26400 (25 preps) | Cat. 26450 (25 preps) | Cat. 26480 (50 preps) |
|---|---|---|---|
| Lysis Buffer E | 15 mL | 15 mL | 2 x 15 mL |
| Wash Solution A | 18 mL | 18 mL | 38 mL |
| Enzyme Incubation Buffer B | 6 mL | 6 mL | 6 mL |
| Elution Buffer H | 6 mL | 6 mL | 6 mL |
| Mini Spin Columns | 25 | 25 | 50 |
| Filter Columns (0.22 μL) | 25 | - | - |
| Filter Columns (0.45 μL) | - | 25 | - |
| Bead Tubes | 25 | 25 | 50 |
| Collection Tubes | 25 | 25 | 50 |
| Elution Tubes (1.7 mL) | 25 | 25 | 50 |
| Product Insert | 1 | 1 | 1 |
Spin Column with Filters (26400, 26450)
| Title | The Source of the River as a Nursery for Microbial Diversity |
| Journal | PLOS ONE. 2015. |
| Authors | Luiz Felipe Valter de Oliveira, Rogério Margis |
| Title | Assessment of the biological invasion risks associated with a massive outdoor cultivation of the green alga, Pseudochoricystis ellipsoidea |
| Journal | Algal Research. 2015. |
| Authors | Izumi Matsuwaki, Shigeaki Harayama, Misako Kato |
| Title | Characteristics of biofilm development in an operating rainwater storage tank. |
| Journal | Environmental Earth Sciences. 2014. |
| Authors | Kim M, Han M. |
| Title | Amplicon pyrosequencing and ion torrent sequencing of wild duck eubacterial microbiome from fecal samples reveals numerous species linked to human and animal diseases. |
| Journal | F1000 Research. 2013. |
| Authors | Strong T, Dowd S, Gutierrez A, Molnar D, Coffman J. |
| Title | Detection of diverse aquatic microbes in blood and organs of drowning victims: first metagenomic approach using high-throughput 454-pyrosequencing. |
| Journal | Forensic Science International. 2012. |
| Authors | Kakizaki E, Ogura Y, Kozawa S, Nishida S, Uchiyama T, Hayashi T, Yukawa N. |
Spin Column (26480)
| Title | The occurrence patterns of gut bacteria in a post-mined peatland, northern Japan |
| Journal | Mires and Pea. 2022. |
| Authors | Shiro Tsuyuzaki, Tamao Saito, Risa S. Arakawa |
| Title | The Source of the River as a Nursery for Microbial Diversity |
| Journal | PLOS ONE. 2015. |
| Authors | Luiz Felipe Valter de Oliveira, Rogério Margis |
| Title | Assessment of the biological invasion risks associated with a massive outdoor cultivation of the green alga, Pseudochoricystis ellipsoidea |
| Journal | Algal Research. 2015. |
| Authors | Izumi Matsuwaki, Shigeaki Harayama, Misako Kato |
| Title | Characteristics of biofilm development in an operating rainwater storage tank. |
| Journal | Environmental Earth Sciences. 2014. |
| Authors | Kim M, Han M. |
| Title | Amplicon pyrosequencing and ion torrent sequencing of wild duck eubacterial microbiome from fecal samples reveals numerous species linked to human and animal diseases. |
| Journal | F1000 Research. 2013. |
| Authors | Strong T, Dowd S, Gutierrez A, Molnar D, Coffman J. |
| Title | Detection of diverse aquatic microbes in blood and organs of drowning victims: first metagenomic approach using high-throughput 454-pyrosequencing. |
| Journal | Forensic Science International. 2012. |
| Authors | Kakizaki E, Ogura Y, Kozawa S, Nishida S, Uchiyama T, Hayashi T, Yukawa N. |
Water RNA/DNA Purification Kits
These kits provide a convenient and rapid method for the isolation of microorganisms from water samples. The kits allows for the rapid isolation and purification of total RNA and DNA simultaneously from the microorganisms found in different types of water samples. The total RNA and DNA (including genomic DNA) are isolated from all the microorganisms found in the water, including bacteria, fungi and algae without the use of any inhibitory organic substances The kit purifies genomic DNA, and all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The purified RNA and DNA are highly concentrated, and can be used directly in a number of downstream applications including PCR, qPCR, RT-PCR, qRT-PCR, Northern blotting, Southern blotting and sequencing reactions.
Water RNA/DNA Purification Regular + Filters Kits
These kits are available with 0.45 μm or 0.22 μm filter formats for capturing microorganisms present in the water.
Water RNA/DNA Purification Regular
This kit is sold without filters columns.
Supporting Data
Kit Specifications
|
Kit Specifications
|
|
| Minimum Water Input |
10 mL
|
| Maximum Water Input |
100 mL
|
| Maximum Filter Column Loading Volume |
20 mL
|
| Maximum Spin Column Loading Volume |
650 µL
|
| Elution Volume |
100 µL
|
| Time to Complete 10 Purifications |
45 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment.
|
Kit Specifications
|
|
| Minimum Water Input |
10 mL
|
| Maximum Water Input |
100 mL
|
| Maximum Filter Column Loading Volume |
20 mL
|
| Maximum Spin Column Loading Volume |
650 µL
|
| Elution Volume |
100 µL
|
| Time to Complete 10 Purifications |
45 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment.
|
Kit Specifications
|
|
| Maximum Spin Column Loading Volume |
650 µL
|
| Elution Volume |
100 µL
|
| Time to Complete 10 Purifications |
45 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 26400 (25 preps) | Cat. 26450 (25 preps) | Cat. 26480 (50 preps) |
|---|---|---|---|
| Lysis Buffer E | 15 mL | 15 mL | 2 x 15 mL |
| Wash Solution A | 18 mL | 18 mL | 38 mL |
| Enzyme Incubation Buffer B | 6 mL | 6 mL | 6 mL |
| Elution Buffer H | 6 mL | 6 mL | 6 mL |
| Mini Spin Columns | 25 | 25 | 50 |
| Filter Columns (0.22 μL) | 25 | - | - |
| Filter Columns (0.45 μL) | - | 25 | - |
| Bead Tubes | 25 | 25 | 50 |
| Collection Tubes | 25 | 25 | 50 |
| Elution Tubes (1.7 mL) | 25 | 25 | 50 |
| Product Insert | 1 | 1 | 1 |
Spin Column with Filters (26400, 26450)
| Title | The Source of the River as a Nursery for Microbial Diversity |
| Journal | PLOS ONE. 2015. |
| Authors | Luiz Felipe Valter de Oliveira, Rogério Margis |
| Title | Assessment of the biological invasion risks associated with a massive outdoor cultivation of the green alga, Pseudochoricystis ellipsoidea |
| Journal | Algal Research. 2015. |
| Authors | Izumi Matsuwaki, Shigeaki Harayama, Misako Kato |
| Title | Characteristics of biofilm development in an operating rainwater storage tank. |
| Journal | Environmental Earth Sciences. 2014. |
| Authors | Kim M, Han M. |
| Title | Amplicon pyrosequencing and ion torrent sequencing of wild duck eubacterial microbiome from fecal samples reveals numerous species linked to human and animal diseases. |
| Journal | F1000 Research. 2013. |
| Authors | Strong T, Dowd S, Gutierrez A, Molnar D, Coffman J. |
| Title | Detection of diverse aquatic microbes in blood and organs of drowning victims: first metagenomic approach using high-throughput 454-pyrosequencing. |
| Journal | Forensic Science International. 2012. |
| Authors | Kakizaki E, Ogura Y, Kozawa S, Nishida S, Uchiyama T, Hayashi T, Yukawa N. |
Spin Column (26480)
| Title | The occurrence patterns of gut bacteria in a post-mined peatland, northern Japan |
| Journal | Mires and Pea. 2022. |
| Authors | Shiro Tsuyuzaki, Tamao Saito, Risa S. Arakawa |
| Title | The Source of the River as a Nursery for Microbial Diversity |
| Journal | PLOS ONE. 2015. |
| Authors | Luiz Felipe Valter de Oliveira, Rogério Margis |
| Title | Assessment of the biological invasion risks associated with a massive outdoor cultivation of the green alga, Pseudochoricystis ellipsoidea |
| Journal | Algal Research. 2015. |
| Authors | Izumi Matsuwaki, Shigeaki Harayama, Misako Kato |
| Title | Characteristics of biofilm development in an operating rainwater storage tank. |
| Journal | Environmental Earth Sciences. 2014. |
| Authors | Kim M, Han M. |
| Title | Amplicon pyrosequencing and ion torrent sequencing of wild duck eubacterial microbiome from fecal samples reveals numerous species linked to human and animal diseases. |
| Journal | F1000 Research. 2013. |
| Authors | Strong T, Dowd S, Gutierrez A, Molnar D, Coffman J. |
| Title | Detection of diverse aquatic microbes in blood and organs of drowning victims: first metagenomic approach using high-throughput 454-pyrosequencing. |
| Journal | Forensic Science International. 2012. |
| Authors | Kakizaki E, Ogura Y, Kozawa S, Nishida S, Uchiyama T, Hayashi T, Yukawa N. |
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